BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0451.p (426 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311352|ref|YP_003775444.1| flagellar basal-body rod protei... 649 0.0 gi|300312833|ref|YP_003776925.1| flagellar hook protein [Herbasp... 603 e-174 gi|300311354|ref|YP_003775446.1| flagellar basal-body rod protei... 77 2e-15 >gi|300311352|ref|YP_003775444.1| flagellar basal-body rod protein FlgE [Herbaspirillum seropedicae SmR1] Length = 432 Score = 649 bits (1673), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/435 (80%), Positives = 382/435 (87%), Gaps = 12/435 (2%) Query: 1 MSFQQGLSGLNGAAKSLDVIGNNVANASTVGFKQSQTQFADMYANSMNRSGNSPVGIGVT 60 M FQQGLSGLNGAAKSLDVIGNNVANASTVGFKQSQ QFADMYANSMNRSGNSPVGIGV+ Sbjct: 1 MGFQQGLSGLNGAAKSLDVIGNNVANASTVGFKQSQAQFADMYANSMNRSGNSPVGIGVS 60 Query: 61 VANVAQQFTQGNITSSNNPLDIAINGDGFFQLAASSSDLSPMYGRNGQFQLDKNGYIVNP 120 V VAQQFTQGNI+SSNNPLDIAINGDGFFQL+AS ++ SPMYGRNGQFQLDK+GYIVNP Sbjct: 61 VPAVAQQFTQGNISSSNNPLDIAINGDGFFQLSASLTNKSPMYGRNGQFQLDKSGYIVNP 120 Query: 121 SMNGAFLMGYAAGVTGGDPVRLQIDTSSIPATTTTTINTKVNLDSRVTVPTTTTFSATDP 180 SMNGA+LMG+AAG GGDPV LQIDTSSIPAT TTTI+TKVNLDSR++VPTTT F+A DP Sbjct: 121 SMNGAYLMGWAAGAQGGDPVPLQIDTSSIPATPTTTISTKVNLDSRLSVPTTTPFNAADP 180 Query: 181 TSYNNSTGVTIYDSLGNPYSVQTFYVKNTPTGTPPTTTWDVYATVDGKTLTTTSGGTTPL 240 TSYN+STGV +YDSLGNPYS+QT+YVK PTGTPPTTTW VYA +D TL + P+ Sbjct: 181 TSYNSSTGVDLYDSLGNPYSMQTYYVKAAPTGTPPTTTWTVYAKIDNTTLNNGATPAVPV 240 Query: 241 KLGSMVFDSTGTLT--SGGA-------MTIDLRSTGTNPGIAKAGGNFASTIALSYTGST 291 LG+M FDSTGT+T +G A +TI T +AKAGG FA+ I +SY+GST Sbjct: 241 ALGTMTFDSTGTMTQINGAAISPTNNTLTISTAQLAT---VAKAGGVFAAPINISYSGST 297 Query: 292 QTGSAFVNLAQSQNGMPPGTLSSFSIDKDGTIVGSYSNQQTKNLGSVVLVNFANPNGLQP 351 QTGSAFVNLAQ QNGMPPG+LSSFS+DKDG+I+GSYSNQQTKNLG+VVLVNFANPNGLQP Sbjct: 298 QTGSAFVNLAQVQNGMPPGSLSSFSVDKDGSIIGSYSNQQTKNLGTVVLVNFANPNGLQP 357 Query: 352 LGNNLYSATAAAGSPLVGKPTTGTFGTLQARAVEDSNVDLTAELVNMIVAQRVYQANSQT 411 LGNNLY A++AAGSPLVGKPTTGTFGTLQARA EDSNVDLTAELVNMIVAQRVYQANSQT Sbjct: 358 LGNNLYQASSAAGSPLVGKPTTGTFGTLQARAKEDSNVDLTAELVNMIVAQRVYQANSQT 417 Query: 412 IKVQDTVLQTLVSLR 426 IKVQDTVLQTLVSLR Sbjct: 418 IKVQDTVLQTLVSLR 432 >gi|300312833|ref|YP_003776925.1| flagellar hook protein [Herbaspirillum seropedicae SmR1] Length = 420 Score = 603 bits (1556), Expect = e-174, Method: Compositional matrix adjust. Identities = 308/426 (72%), Positives = 356/426 (83%), Gaps = 6/426 (1%) Query: 1 MSFQQGLSGLNGAAKSLDVIGNNVANASTVGFKQSQTQFADMYANSMNRSGNSPVGIGVT 60 MSFQQGLSGL+GA+K LDVIGNNVANASTVGFKQ Q QFAD YANSMNRSGNSPVGIGVT Sbjct: 1 MSFQQGLSGLHGASKHLDVIGNNVANASTVGFKQGQVQFADAYANSMNRSGNSPVGIGVT 60 Query: 61 VANVAQQFTQGNITSSNNPLDIAINGDGFFQLAASSSDLSPMYGRNGQFQLDKNGYIVNP 120 VANV+Q FTQGNI ++NN LD+AINGDGFF++AAS +D SP YGRNGQFQL+K+GY++NP Sbjct: 61 VANVSQAFTQGNIATTNNALDVAINGDGFFRMAASLTDQSPRYGRNGQFQLNKDGYLINP 120 Query: 121 SMNGAFLMGYAAGVTGGDPVRLQIDTSSIPATTTTTINTKVNLDSRVTVPTTTTFSATDP 180 SMNGA+L GY AG GGDPV L+IDT+++PAT TT ++TKVNLDSR +VPT + F +P Sbjct: 121 SMNGAYLTGYPAGTNGGDPVPLKIDTATLPATATTRVSTKVNLDSRKSVPTVSPFDPANP 180 Query: 181 TSYNNSTGVTIYDSLGNPYSVQTFYVKNTPTGTPPTTTWDVYATVDGKTLTTTSGGTTPL 240 SY+NSTG T+YDSLGNPYS+QTFYVK TGTPP+TTW VYAT+DGKT+ T P Sbjct: 181 NSYSNSTGATVYDSLGNPYSLQTFYVKGAATGTPPSTTWSVYATLDGKTIINAPPATGPQ 240 Query: 241 KLGSMVFDSTGTLTSGGAMTIDLRSTGTNPGIAKAGGNFASTIALSYTGSTQTGSAFVNL 300 LG++ FD+TG LT+ M+++L + +AK G F IAL Y GSTQTGSAF NL Sbjct: 241 LLGTLRFDATGALTASTGMSLNLST------LAKGGATFPGPIALGYAGSTQTGSAFANL 294 Query: 301 AQSQNGMPPGTLSSFSIDKDGTIVGSYSNQQTKNLGSVVLVNFANPNGLQPLGNNLYSAT 360 AQSQ+GMPPGTLSSFSI KDG+I+G+YSNQQT+ +G +VLVNFANP+GLQP+GNNLY A+ Sbjct: 295 AQSQDGMPPGTLSSFSIGKDGSIIGAYSNQQTRVMGQIVLVNFANPHGLQPIGNNLYQAS 354 Query: 361 AAAGSPLVGKPTTGTFGTLQARAVEDSNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQ 420 AAGS LVG PTTG FG LQ RAVE SNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQ Sbjct: 355 EAAGSALVGTPTTGAFGALQGRAVEQSNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQ 414 Query: 421 TLVSLR 426 TLVSLR Sbjct: 415 TLVSLR 420 >gi|300311354|ref|YP_003775446.1| flagellar basal-body rod protein FlgG [Herbaspirillum seropedicae SmR1] Length = 260 Score = 77.4 bits (189), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 47/125 (37%), Positives = 66/125 (52%), Gaps = 13/125 (10%) Query: 8 SGLNGAAKSLDVIGNNVANASTVGFKQSQTQFADMYANSMNRSGNS-----------PVG 56 +GL+ LDVI NN+AN ST GFK+S+ F D+ ++ + G +G Sbjct: 10 TGLDAQQTQLDVISNNLANVSTNGFKRSRAVFEDLLYQTVRQPGAQSSQQTQLPSGLQIG 69 Query: 57 IGVTVANVAQQFTQGNITSSNNPLDIAINGDGFFQLAASSSDLSPMYGRNGQFQLDKNGY 116 GV + FTQGN+ +NN D+AI G+GFFQ+ D + Y R+G FQ D G Sbjct: 70 TGVRPVATERIFTQGNVNQTNNDKDLAIQGNGFFQILL--PDGTTAYTRDGAFQTDSQGQ 127 Query: 117 IVNPS 121 +V S Sbjct: 128 LVTSS 132 Score = 74.7 bits (182), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 46/123 (37%), Positives = 66/123 (53%), Gaps = 5/123 (4%) Query: 308 PPGTL----SSFSIDKDGTI-VGSYSNQQTKNLGSVVLVNFANPNGLQPLGNNLYSATAA 362 PP T+ + ++ +DG + + + T +G++ + F NP GL+ G NLY TAA Sbjct: 138 PPITVPLNTTKLTVGRDGVVSIMQAGSTATTQIGTIQVATFINPAGLESRGENLYMETAA 197 Query: 363 AGSPLVGKPTTGTFGTLQARAVEDSNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQTL 422 +G+P P T G L VE SNV++ ELVNMI QR Y+ NS+ I D +L L Sbjct: 198 SGAPNPNNPGTNGAGALWQGYVETSNVNVVEELVNMIQTQRAYEINSKAITTSDQMLAKL 257 Query: 423 VSL 425 L Sbjct: 258 SQL 260 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.310 0.126 0.351 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,049,735 Number of extensions: 39111 Number of successful extensions: 129 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 127 Number of HSP's successfully gapped: 5 Length of query: 426 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 338 Effective length of database: 1,203,190 Effective search space: 406678220 Effective search space used: 406678220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 132 (55.5 bits)