BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0442.p (466 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311360|ref|YP_003775452.1| outer membrane drug efflux lipo... 628 0.0 gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [... 203 2e-53 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 174 2e-44 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 171 8e-44 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 160 1e-40 >gi|300311360|ref|YP_003775452.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 470 Score = 628 bits (1619), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/456 (71%), Positives = 390/456 (85%) Query: 5 LSFPPPSRLALLLALALLGGCSSLVRTPYTAPVTQTPAAWQQRDPAAAVNASRWWQGFGD 64 +S P P RL+LLLAL LLG CS L R+ YT TQTPA+WQQR + RWWQGFGD Sbjct: 6 MSLPVPFRLSLLLALTLLGACSPLTRSAYTPVSTQTPASWQQRGSQPEADVQRWWQGFGD 65 Query: 65 ETLSALIDEALRKNNNLALATIKVRRAQLQAGIANDAFVPALSGQVGSTASKALDGNRQT 124 TL+ALIDEALRKNN+LALA IK+RRAQ QAG+A+DAFVPALS +G+++SK LDG+R + Sbjct: 66 ATLTALIDEALRKNNDLALAAIKLRRAQAQAGLADDAFVPALSASLGASSSKQLDGDRIS 125 Query: 125 ARSSQNSLSLSYEVDLWGRLGANYDVARWEALATEQDRAATALSLVGTTASLYWTGAYLN 184 R+SQ+S+ LSYEVDLWGRL A D++RWEALATEQDR +TAL+L+GTTA+LYWTGAYLN Sbjct: 126 TRASQSSVKLSYEVDLWGRLAAASDLSRWEALATEQDRQSTALTLIGTTANLYWTGAYLN 185 Query: 185 QRLRTAEQSIAYAQKTLDLINTQYRAGAVSSVDVLTAQQNLLSQQASYTELVQQQVENDN 244 QR+ TAEQSIAYA+KTL+L+ TQY+AGAVSSV+VL+AQQNL SQQ+SYTELVQQ+VENDN Sbjct: 186 QRVATAEQSIAYAEKTLELVRTQYQAGAVSSVEVLSAQQNLYSQQSSYTELVQQRVENDN 245 Query: 245 ALAILFDSPPGQRRSLPPALPEQPLPVLEAGLPAELLARRPDLQAAELRLRESLANVDLT 304 ALAILFD PPG+ +LP LPEQ LPVL AG+PAELL RRPDL+A ELR+R +LA VD Sbjct: 246 ALAILFDQPPGKAIALPARLPEQALPVLAAGVPAELLGRRPDLRALELRIRSALAGVDQA 305 Query: 305 RASFYPKLSLTGSLGTSSDALLRLVQNPVASLAAQLSLPFLQQTQMKLQIKVSQADYESA 364 R ++YP+LSLTG+LGTSSDALLRLVQNPVA+L L+LPFLQ ++MK ++ +SQAD+++A Sbjct: 306 RLNYYPQLSLTGTLGTSSDALLRLVQNPVATLGGALTLPFLQHSEMKFKVGISQADFDTA 365 Query: 365 VTQFRQTLYAAFSDVENALSARTQYARQQALLARNVEVARAAEQLYELRYRAGSVTLQSW 424 V FR+ LY AFS+VENALSAR QYARQQALL RN++ A+AAE+L E+RYRAGS TLQ W Sbjct: 366 VLNFRKGLYTAFSEVENALSARQQYARQQALLVRNLDAAQAAERLLEVRYRAGSATLQLW 425 Query: 425 LDQQDKRRSAENTLAQVRLNQLKNQMTLYQALGGDA 460 LDQQDKRR+AE +LAQVRLNQLKNQMTLYQALGGDA Sbjct: 426 LDQQDKRRTAEISLAQVRLNQLKNQMTLYQALGGDA 461 >gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [Herbaspirillum seropedicae SmR1] Length = 492 Score = 203 bits (517), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 153/446 (34%), Positives = 228/446 (51%), Gaps = 13/446 (2%) Query: 23 GGCSSLVRTPYTAPV--TQTPAAWQQRDPAAAVNASR------WWQGFGDETLSALIDEA 74 GGC+ +R T P+ Q P W A +A + WW+ FG LS L+++ Sbjct: 33 GGCA--MREVQTDPILEMQVPDKWGGGASADVAHAEQLWPDTDWWKQFGSNELSELVEQG 90 Query: 75 LRKNNNLALATIKVRRAQLQAGIANDAFVPALSGQVGSTASKALDGNRQTARSSQNSLSL 134 R N +A A +V++A+ QA IA +P+ G+ + G T ++ SL + Sbjct: 91 QRNNYEIAAAYSRVKQAEAQARIAGAPLLPSAGINAGAAREIPISGGTPT-NTANASLQV 149 Query: 135 SYEVDLWGRLGANYDVARWEALATEQDRAATALSLVGTTASLYWTGAYLNQRLRTAEQSI 194 SYE+D WG+ A + A A DR AL++ S Y L RL A Q++ Sbjct: 150 SYEIDFWGKNRAGVEAAEATLRANRYDRETVALTVTSGIVSTYLQVLSLRDRLAIARQNV 209 Query: 195 AYAQKTLDLINTQYRAGAVSSVDVLTAQQNLLSQQASYTELVQQQVENDNALAILFDSPP 254 A++ L L+ Q RAG+ S +D+ + L +Q+ +L QQ+ + ALAIL PP Sbjct: 210 QNAERVLRLVEAQSRAGSASPLDLARQRSALANQRQLIPDLQQQEQDAQTALAILLGRPP 269 Query: 255 GQRRSLPPALPEQPLPVLEAGLPAELLARRPDLQAAELRLRESLANVDLTRASFYPKLSL 314 + L +P + AGLP+ELL RRPD++ AE L + ANV + RA+ +P ++L Sbjct: 270 QNFKVEEKGLARIQMPRISAGLPSELLTRRPDIRRAEASLAAANANVAVARAALFPSITL 329 Query: 315 TGSLGTSSDALLRLVQNP--VASLAAQLSLPFLQQTQMKLQIKVSQADYESAVTQFRQTL 372 TGS G S ALL L P A+L A L P +++ Q ++ A E V +RQ + Sbjct: 330 TGSTGAQSSALLSLFDGPNLFANLGAGLIAPIFDGGKLRGQRDLAIAQKEELVQVYRQRV 389 Query: 373 YAAFSDVENALSARTQYARQQALLARNVEVARAAEQLYELRYRAGSVTLQSWLDQQDKRR 432 + S+VE AL A + L V+ AR A +L E+RYRAG+ L LD Q Sbjct: 390 ITSLSEVEKALGAIRSLEERYRLKVGEVDQARFAFELAEIRYRAGAEDLLVVLDTQRTLS 449 Query: 433 SAENTLAQVRLNQLKNQMTLYQALGG 458 A+N L Q++L +L+ ++LY+ALGG Sbjct: 450 EAQNQLGQIKLQRLQATVSLYKALGG 475 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 174 bits (440), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 158/480 (32%), Positives = 243/480 (50%), Gaps = 39/480 (8%) Query: 5 LSFPPPSRL----ALLLALALLGGCSSLVRTPYT---APVTQT-PAAWQQRDPAAAVNAS 56 LS P RL A L+ +LGGCS + Y APV + PA R AVN Sbjct: 4 LSMPRSPRLLHMCAALVMAGVLGGCS--LAPTYERPQAPVESSYPADAAGRAELQAVNLG 61 Query: 57 RWWQGFGDETLSALIDEALRKNNNLALATIKVRRAQLQAGIANDAFVPAL--SGQVGST- 113 W Q F D+ L LI AL N +L A + + A+ Q IA +P L SG T Sbjct: 62 -WRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTR 120 Query: 114 --ASKALDGNRQTARSSQNSLSL-SYEVDLWGRLGANYDVARWEALATEQDRAATALSLV 170 AS++L G + S Q L++ +YE+D +GR+ D A L+TE+ + + +SLV Sbjct: 121 TAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLV 180 Query: 171 GTTASLYWTGAYLNQRLRTAEQSIAYAQKTLDLINTQYRAGAVSSVDVLTAQQNLLSQQA 230 A Y ++L+ A++++ Q LDL + GA +++D Q + S + Sbjct: 181 AQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARV 240 Query: 231 SYTELVQQQVENDNALAILFDSPPGQRRSLPPA---LPEQPLPVLEAGLPAELLARRPDL 287 + T+L +Q+ + +NAL +L G+ LPPA E+ + + AGLP++LL RPD+ Sbjct: 241 AKTQLERQRAQAENALTLLVGKKIGE---LPPAQLLSDEKIVTDIPAGLPSDLLTNRPDI 297 Query: 288 QAAELRLRESLANVDLTRASFYPKLSLTGSLGTSSDALLRLVQNPVA--SLAAQLSLPFL 345 ++AE L + AN+ RA+F+P++SLTG+ G++S L L ++ S LSLP Sbjct: 298 RSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIF 357 Query: 346 QQTQMKLQIKVSQADYESAVTQFRQTLYAAFSDVENALSAR----TQYARQQALLARNVE 401 + + +++ AV + + + AF +V +AL AR Q Q+A LA E Sbjct: 358 DFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQE 417 Query: 402 VARAAEQLYELRYRAGSVTLQSWLDQQDKRR---SAENTLAQVRLNQLKNQMTLYQALGG 458 + L +LR++ G + S LDQ D +R SA+ L Q R +L N + LY+ALGG Sbjct: 418 RLK----LTDLRFKNG---IASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGG 470 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 171 bits (434), Expect = 8e-44, Method: Compositional matrix adjust. Identities = 146/420 (34%), Positives = 223/420 (53%), Gaps = 22/420 (5%) Query: 58 WWQGFGDETLSALIDEALRKNNNLALATIKVRRAQLQAGIANDAFVPALSGQVGSTASKA 117 W F D L ALI +AL + +L A ++V+ AQ GI + P + Q G S+ Sbjct: 66 WRDYFTDPPLQALITQALENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRT 125 Query: 118 ---LDGNRQ--TARSSQNSLSLS-YEVDLWGRLGANYDVARWEALATEQDRAATALSLVG 171 L+ R+ + Q L LS +E+DLWGRL + D A A A++ R+A ALSL+ Sbjct: 126 PADLNLTRRPLVGSAYQAGLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMA 185 Query: 172 TTASLYWTGAYLNQRLRTAEQSIAYAQKTLDLINTQYRAGAVSSVDVLTAQQNLLSQQAS 231 A + A L++RL A Q+IA Q++ + + + GA S +++ + L QA Sbjct: 186 QVADAWLELAELDERLEIANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQAL 245 Query: 232 YTELVQQQVENDNALAILFDS----PPGQRRSLPPAL--PEQPLPVLEAGLPAELLARRP 285 +L QQ+ + NAL +L S P GQ R+ A+ P QP GLPA LL RRP Sbjct: 246 GAQLQQQRAQRLNALTLLVGSPVVLPVGQARAGLDAVFAPLQP------GLPALLLTRRP 299 Query: 286 DLQAAELRLRESLANVDLTRASFYPKLSLTGSLGTSSDALLRLVQNPVA--SLAAQLSLP 343 D+ AAE RL + AN+ RA+F+P +SLTG++GT+S L L ++ + + ++LP Sbjct: 300 DVAAAEHRLMAANANIGAARAAFFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLP 359 Query: 344 FLQQTQMKLQIKVSQADYESAVTQFRQTLYAAFSDVENALSARTQYARQQALLARN-VEV 402 +++ + +S+ + AV + +T+ AF +V +AL+AR Q+ QQ +A++ V V Sbjct: 360 IFTAGRLRNNLALSEVRRDLAVAAYEKTVQTAFREVADALAAR-QWLTQQLEIAQSAVRV 418 Query: 403 ARAAEQLYELRYRAGSVTLQSWLDQQDKRRSAENTLAQVRLNQLKNQMTLYQALGGDARA 462 R +L LRY G+ LD Q SA+ L Q+R L + + LY ALGG+ A Sbjct: 419 QRERARLSALRYDNGAAPFLDVLDAQRDLLSAQQQLVQIRRALLSSSVALYAALGGEGVA 478 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 160 bits (406), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 140/472 (29%), Positives = 230/472 (48%), Gaps = 28/472 (5%) Query: 10 PSRLALLLALALLGGCSSL--VRTPYTAPVTQTPAAWQQRDPAAAVNAS-----RWWQGF 62 P L ++LA+ LLGGC+ + P APV A ++ A A +A W F Sbjct: 13 PMMLPVMLAM-LLGGCTLMPDYHRP-DAPVAAHFAGADSKNAAGASSAPVVADIGWRDVF 70 Query: 63 GDETLSALIDEALRKNNNLALATIKVRRAQLQAGIANDAFVPALSGQVGSTASK-----A 117 D +L +I AL N +L +A + + +A+ Q G+ A P++ TAS+ + Sbjct: 71 TDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETASRTPGDLS 130 Query: 118 LDGNRQTARSSQNSLSLS-YEVDLWGRLGANYDVARWEALATEQDRAATALSLVGTTASL 176 G+ T+RS +L S YE+DL+GR+ + + A L+T + R +T +SL+ A+ Sbjct: 131 STGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATA 190 Query: 177 YWTGAYLNQRLRTAEQSIAYAQKTLDLINTQYRAGAVSSVDVLTAQQNLLSQQASYTELV 236 + T A RLR A+ ++ + L + G+ S++ + AQ ++ S + Sbjct: 191 WLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESARVDVESYT 250 Query: 237 QQQVENDNALAILF--DSPPGQRRSLPPALPE------QPLPVLEAGLPAELLARRPDLQ 288 Q ++ NAL +L D P LP LP+ PL + GLP++LL RR D+ Sbjct: 251 AQVDQDRNALVLLVGKDLPTAL---LPQGLPDTRLAAASPLATIPPGLPSDLLQRRADIV 307 Query: 289 AAELRLRESLANVDLTRASFYPKLSLTGSLGTSSDALLRLVQNPVAS--LAAQLSLPFLQ 346 AE LR + AN+ RA+FYP ++LT + GT+S L L + S Q+SLP Sbjct: 308 QAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQISLPIFD 367 Query: 347 QTQMKLQIKVSQADYESAVTQFRQTLYAAFSDVENALSARTQYARQQALLARNVEVARAA 406 + + ++ + +V Q+ + + AF +V +AL+ R RQ V+ + A Sbjct: 368 GGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQASDEA 427 Query: 407 EQLYELRYRAGSVTLQSWLDQQDKRRSAENTLAQVRLNQLKNQMTLYQALGG 458 +L + RY +G + + LD Q SA+ L +L++ N +T Y+ LGG Sbjct: 428 FRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGG 479 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.126 0.350 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,216,838 Number of extensions: 46220 Number of successful extensions: 294 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 233 Number of HSP's successfully gapped: 17 Length of query: 466 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 377 Effective length of database: 1,198,455 Effective search space: 451817535 Effective search space used: 451817535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)