BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0437.p (180 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311362|ref|YP_003775454.1| HlyD family secretion, possible... 210 5e-56 gi|300312743|ref|YP_003776835.1| membrane-fusion protein [Herbas... 69 2e-13 >gi|300311362|ref|YP_003775454.1| HlyD family secretion, possible efflux component [Herbaspirillum seropedicae SmR1] Length = 407 Score = 210 bits (535), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 113/140 (80%), Positives = 126/140 (90%) Query: 1 MNIAYFRRPKILLLSLAVLLAGGYLVHRALTPPAPPSYLTATASVADIQDVVLASGTVKA 60 M +A RRPK + L+L VLL +++ LTPPAPP+YL+ATA VADIQDVVLASGTVKA Sbjct: 3 MKLAQLRRPKFIFLALLVLLIAAWIIRSVLTPPAPPTYLSATARVADIQDVVLASGTVKA 62 Query: 61 YKQVSVGAQVSGQIKSLKVALGERVKKGQLVAEIDSLTQANALASAEYSLQNLQAQLRAK 120 YKQVSVGAQVSGQIKSLKVALG++VKKGQLVAEIDSLTQANALASAE+SLQNLQAQLRAK Sbjct: 63 YKQVSVGAQVSGQIKSLKVALGDQVKKGQLVAEIDSLTQANALASAEFSLQNLQAQLRAK 122 Query: 121 EATLKQAQLAYARQQMMLAA 140 EA+LKQAQLAYARQ+MMLA Sbjct: 123 EASLKQAQLAYARQKMMLAG 142 >gi|300312743|ref|YP_003776835.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 69.3 bits (168), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 46/140 (32%), Positives = 74/140 (52%), Gaps = 6/140 (4%) Query: 14 LSLAVLLAGGYLVH------RALTPPAPPSYLTATASVADIQDVVLASGTVKAYKQVSVG 67 ++L ++L GGY ++ P P T T AD++ VV A+G V A V VG Sbjct: 28 VALVIVLGGGYWLYSRQQPPPPAPKPIPVKLETVTVERADLEQVVNATGNVVAQDYVDVG 87 Query: 68 AQVSGQIKSLKVALGERVKKGQLVAEIDSLTQANALASAEYSLQNLQAQLRAKEATLKQA 127 ++V+GQI + V +GE VK G+L+A + Q++ + + +L L+A+L + A L A Sbjct: 88 SKVAGQISDVDVVVGETVKAGKLLATVAPAVQSSRIENNRATLARLKAELAGQNAQLDFA 147 Query: 128 QLAYARQQMMLAATPARART 147 QL + RQ + A R + Sbjct: 148 QLQFQRQTQLKAENATREES 167 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.127 0.349 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 281,066 Number of extensions: 8052 Number of successful extensions: 88 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 88 Number of HSP's successfully gapped: 2 Length of query: 180 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 101 Effective length of database: 1,245,805 Effective search space: 125826305 Effective search space used: 125826305 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)