BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0353.p (456 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311416|ref|YP_003775508.1| gluconate transporter family pr... 869 0.0 gi|300310424|ref|YP_003774516.1| high-affinity gluconate permeas... 334 8e-93 gi|300311081|ref|YP_003775173.1| high-affinity gluconate transpo... 332 3e-92 >gi|300311416|ref|YP_003775508.1| gluconate transporter family protein [Herbaspirillum seropedicae SmR1] Length = 456 Score = 869 bits (2246), Expect = 0.0, Method: Compositional matrix adjust. Identities = 452/456 (99%), Positives = 454/456 (99%) Query: 1 LISTHLAAWAAQDTQLLLVTLAALVTIVVLISFLSIAPFLSILIGTFVAGIGAGLPLEDI 60 +ISTHLAAWAAQDTQLLLVTL ALVTIVVLISFLSIAPFLSILIGTFVAGIGAGLPLEDI Sbjct: 1 MISTHLAAWAAQDTQLLLVTLTALVTIVVLISFLSIAPFLSILIGTFVAGIGAGLPLEDI 60 Query: 61 AKAFSKGAGSLLGEAGIIIALGAMLGALMAESGAADRIVNTLLRYARGSIIPWMMALVAL 120 AKAFSKGAGSLLGEAGIIIALGAMLGALMAESGAADRIVNTLLRYARGS IPWMMALVAL Sbjct: 61 AKAFSKGAGSLLGEAGIIIALGAMLGALMAESGAADRIVNTLLRYARGSFIPWMMALVAL 120 Query: 121 VVGLPLFFEVGLVMMAPIIFVMARRSELPIMRVAIPALAGMTTLHALLPPHPGPLIAVSA 180 VVGLPLFFEVGLVMMAPIIFVMARRSELPIMRVAIPALAGMTTLHALLPPHPGPLIAVSA Sbjct: 121 VVGLPLFFEVGLVMMAPIIFVMARRSELPIMRVAIPALAGMTTLHALLPPHPGPLIAVSA 180 Query: 181 LHADLGTTMLLGFIVAIPAVIIAGPLYGNFIAPRLNLAEPDQIGKLFSAREGQSQPSFLV 240 LHADLGTTMLLGFIVAIPAVIIAGPLYGNFIAPRLNLAEPDQIGKLFSAREGQSQPSFLV Sbjct: 181 LHADLGTTMLLGFIVAIPAVIIAGPLYGNFIAPRLNLAEPDQIGKLFSAREGQSQPSFLV 240 Query: 241 ALVTILLPVVLMLGRTVARIWVTPKTELFEMLNFFGEPIIALTVTVLFAVVVLGWGQGSS 300 ALVTILLPVVLMLGRTVARIWVTPKTELFEMLNFFGEPIIALTVTVLFAVVVLGWGQGSS Sbjct: 241 ALVTILLPVVLMLGRTVARIWVTPKTELFEMLNFFGEPIIALTVTVLFAVVVLGWGQGSS 300 Query: 301 RFEVGATLRRALPPIAGLLLTIGAGGGLKQTLLSAGISDTITKIADGTHMPLILLAWIIA 360 RFEVGATLRRALPPIAGLLLTIGAGGGLKQTLLSAGISDTITKIADGTHMPLILLAWIIA Sbjct: 301 RFEVGATLRRALPPIAGLLLTIGAGGGLKQTLLSAGISDTITKIADGTHMPLILLAWIIA 360 Query: 361 VALRQATGSATVATTATAGIVAPLVAGLSATHNSLMALAIGAGSVFFCHVNDAGFWMVKE 420 VALRQATGSATVATTATAGIVAPLVAGLSATHNSLMALAIGAGSVFFCHVNDAGFWMVKE Sbjct: 361 VALRQATGSATVATTATAGIVAPLVAGLSATHNSLMALAIGAGSVFFCHVNDAGFWMVKE 420 Query: 421 YFGLNLKQTLATWSVIQTLVSVIGLGMTLLLWGLLV 456 YFGLNLKQTLATWSVIQTLVSVIGLGMTL+LWGLLV Sbjct: 421 YFGLNLKQTLATWSVIQTLVSVIGLGMTLVLWGLLV 456 >gi|300310424|ref|YP_003774516.1| high-affinity gluconate permease [Herbaspirillum seropedicae SmR1] Length = 450 Score = 334 bits (857), Expect = 8e-93, Method: Compositional matrix adjust. Identities = 183/442 (41%), Positives = 291/442 (65%), Gaps = 1/442 (0%) Query: 11 AQDTQLLLVTLAALVTIVVLISFLSIAPFLSILIGTFVAGIGAGLPLEDIAKAFSKGAGS 70 + D LL + A++ ++++I+ L I PF+++ I + + G+ +G+PL I K+F G G Sbjct: 5 SHDATLLFNAVIAILALILMITRLRIHPFIALTITSGLLGLISGMPLPKIVKSFQDGFGG 64 Query: 71 LLGEAGIIIALGAMLGALMAESGAADRIVNTLLRYARGSIIPWMMALVALVVGLPLFFEV 130 +LG GI++ LG MLG LMAESG AD+I TL+R + W M + A +VG+PLFFE+ Sbjct: 65 VLGFVGIVLGLGTMLGKLMAESGGADQIAQTLVRAFGRERVHWAMMMGAFLVGIPLFFEI 124 Query: 131 GLVMMAPIIFVMARRSELPIMRVAIPALAGMTTLHALLPPHPGPLIAVSALHADLGTTML 190 G V++ P++F++ARRS +P+ ++ +P LA ++ +H L+PPHPGPL+A+ AD+G T+ Sbjct: 125 GFVLLIPLVFIVARRSGVPLFKIGMPMLASLSVMHGLVPPHPGPLLAIGIFGADIGKTIF 184 Query: 191 LGFIVAIPAVIIAGPLYGNFIAPRLNLAEPDQI-GKLFSAREGQSQPSFLVALVTILLPV 249 G +V +P VIIAGP++G FI+ +++ +++ +L + P F L+T+L PV Sbjct: 185 YGLLVGLPTVIIAGPMFGAFISRYVDIKPSEELMAQLVREPQTTKLPGFATTLITVLAPV 244 Query: 250 VLMLGRTVARIWVTPKTELFEMLNFFGEPIIALTVTVLFAVVVLGWGQGSSRFEVGATLR 309 LML +T+ + + + ++++F G PI+AL +L A+ G +G +R +V + Sbjct: 245 FLMLLKTLVDVTLPEGHAMRDLMDFIGNPIVALLAALLLAIWTFGIARGFTRQQVLKFVD 304 Query: 310 RALPPIAGLLLTIGAGGGLKQTLLSAGISDTITKIADGTHMPLILLAWIIAVALRQATGS 369 ++L P A ++L IGAGGG KQ L+++G+ I ++A + +LLAW +A +R ATGS Sbjct: 305 QSLAPTAAIVLIIGAGGGFKQMLVNSGVGTAIGQLAVHAQISPLLLAWFVAGVIRVATGS 364 Query: 370 ATVATTATAGIVAPLVAGLSATHNSLMALAIGAGSVFFCHVNDAGFWMVKEYFGLNLKQT 429 ATVAT AGIVAPLVA + T+ L+ LA GAGS+ HVNDAGFW+VKEYF +++ +T Sbjct: 365 ATVATITGAGIVAPLVALIPGTNKELLVLATGAGSLILSHVNDAGFWLVKEYFNMSVGET 424 Query: 430 LATWSVIQTLVSVIGLGMTLLL 451 TW+V++TL+SV+ + +LL Sbjct: 425 FKTWTVMETLISVVAIIFIMLL 446 >gi|300311081|ref|YP_003775173.1| high-affinity gluconate transporter (GNT-III system) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 332 bits (852), Expect = 3e-92, Method: Compositional matrix adjust. Identities = 180/436 (41%), Positives = 266/436 (61%), Gaps = 4/436 (0%) Query: 10 AAQDTQLLLVTLAALVTIVVLISFLSIAPFLSILIGTFVAGIGAGLPLEDIAKAFSKGAG 69 A Q + LL+ L A++ ++VLI+ + PF+ +++ + V G+ G+P+ +I KAF G G Sbjct: 3 AVQGSALLVYALVAVIALIVLIAKFKMNPFIVLIVVSLVLGLAVGMPMGNIVKAFETGVG 62 Query: 70 SLLGEAGIIIALGAMLGALMAESGAADRIVNTLLRYARGSIIPWMMALVALVVGLPLFFE 129 + LG +++ LG MLG +MAESG A+RI NT+++ + W M VA +VGLP+FFE Sbjct: 63 NALGHIALVVGLGTMLGKMMAESGGAERIANTMIKAFGEKNVHWAMMTVAFIVGLPVFFE 122 Query: 130 VGLVMMAPIIFVMARRSELPIMRVAIPALAGMTTLHALLPPHPGPLIAVSALHADLGTTM 189 VG V++ PI F +A+R+ ++ V IP +AG++ +H L+PPHP L+AV+A AD+G T+ Sbjct: 123 VGFVLLVPIAFNVAKRTGTNMVLVGIPMVAGLSVVHGLIPPHPAALLAVTAYSADIGRTI 182 Query: 190 LLGFIVAIPAVIIAGPLYGNFIAPRLNLAEPDQ--IGKLFS-AREGQSQPSFLVALVTIL 246 L IV IP IIAGP++G I+ ++ + PD I + ++ + P F + L TIL Sbjct: 183 LYALIVGIPTAIIAGPIFGKLIS-KVVIPNPDNPLISQFVDEGKKDRELPGFGITLFTIL 241 Query: 247 LPVVLMLGRTVARIWVTPKTELFEMLNFFGEPIIALTVTVLFAVVVLGWGQGSSRFEVGA 306 LPV LML + A ++ PKT + L G +IAL + L + G +G ++ Sbjct: 242 LPVALMLIGSWADLFFAPKTFANDFLRLIGNSVIALLIATLVSFWTFGRARGFGADQILK 301 Query: 307 TLRRALPPIAGLLLTIGAGGGLKQTLLSAGISDTITKIADGTHMPLILLAWIIAVALRQA 366 L PIA + L +GAG G + L+ G+S I IA H+ ++L W +A +R A Sbjct: 302 FTNECLAPIASITLVVGAGAGFGRILMDGGVSKAIVGIATDAHLSPLILGWFVAALIRVA 361 Query: 367 TGSATVATTATAGIVAPLVAGLSATHNSLMALAIGAGSVFFCHVNDAGFWMVKEYFGLNL 426 TGSATVA T GIVAP+V+ + LM LA GAGS+ HVND GFW+VKEYF + + Sbjct: 362 TGSATVAMTTACGIVAPIVSTVGGVRPELMVLATGAGSLILSHVNDGGFWLVKEYFNMTV 421 Query: 427 KQTLATWSVIQTLVSV 442 QT TW+V++TLVSV Sbjct: 422 PQTFKTWTVMETLVSV 437 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 973,502 Number of extensions: 30839 Number of successful extensions: 232 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 229 Number of HSP's successfully gapped: 3 Length of query: 456 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 368 Effective length of database: 1,203,190 Effective search space: 442773920 Effective search space used: 442773920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)