BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0346.p (487 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspiri... 804 0.0 gi|300312564|ref|YP_003776656.1| outer membrane multidrug resist... 273 2e-74 gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspiri... 236 4e-63 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 156 4e-39 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 143 4e-35 >gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 487 Score = 804 bits (2077), Expect = 0.0, Method: Compositional matrix adjust. Identities = 460/487 (94%), Positives = 476/487 (97%) Query: 1 MTRIAFSKLPALTVIALSLTIAGCADQGGIQPQAQLRDVAVLDAGQAIRQAASEAGWPQR 60 MTRIAFSKLP LTVIALSLT+AGCADQGGIQPQAQ+RDVAVLDAGQAIRQAASEAGWPQR Sbjct: 1 MTRIAFSKLPVLTVIALSLTMAGCADQGGIQPQAQVRDVAVLDAGQAIRQAASEAGWPQR 60 Query: 61 AWWQAYGDAQLNQLVTRAVAGNPSMAVAAARVRQAQSMAVVAHAGELPSAQLDVSVGRKD 120 AWWQAYGDAQL+QLVTRAVAGNPSMAVAAARVRQAQSMAVVAHAGELPSAQLDVSVGRKD Sbjct: 61 AWWQAYGDAQLDQLVTRAVAGNPSMAVAAARVRQAQSMAVVAHAGELPSAQLDVSVGRKD 120 Query: 121 WSDNVYYGAAFRDKLTWNNTATLGLSYNVDLWDRNKNATLRAEDEMHAVAADARAAQLPP 180 WSDNVYYGAAFR+KLTWNNTATLGLSYNVDLWDRNKNATLRA+DEMHAVAADARAAQL Sbjct: 121 WSDNVYYGAAFRNKLTWNNTATLGLSYNVDLWDRNKNATLRAQDEMHAVAADARAAQLDL 180 Query: 181 PPPXLRTYVQLALQYGLLDNTEAMLKEEQKILDLAQRRFKGGLGTQLEITQAESPLPETR 240 +RTYVQLALQYGLLDNTEA+LKEEQKILDLA RRFKGGLGTQLEITQAESPLPETR Sbjct: 181 EGNVVRTYVQLALQYGLLDNTEALLKEEQKILDLAHRRFKGGLGTQLEITQAESPLPETR 240 Query: 241 RQMEALQENIALIRNQLAALLGAGPGAADGITRPTMQLQTALGLPSALPADLVGRRPDIS 300 RQ+EAL ENIALIRNQLAALLGAGPGAADG+TRP MQLQT+LGLPSALPADLVGRRPDIS Sbjct: 241 RQIEALHENIALIRNQLAALLGAGPGAADGVTRPGMQLQTSLGLPSALPADLVGRRPDIS 300 Query: 301 AARWRVEAAAKGISVAKAAFYPNINLLAGIGPAAAGGGLFSFLSAPNLQTTYGPAISLPI 360 AARWRVEAAAKGISVAKAAFYPNINLLAGIGPAAAGGGLFSFLSAPNLQTTYGPAISLPI Sbjct: 301 AARWRVEAAAKGISVAKAAFYPNINLLAGIGPAAAGGGLFSFLSAPNLQTTYGPAISLPI 360 Query: 361 FEGGRLRGQLGAASAGYDIEVEHYNALLSQALKGISDQVVTLQSVARQQKQAQESVQAAQ 420 FEGGRLRGQLGAASAGYDIEVEHYNALLSQALKGISDQVVTLQSVARQQ+QAQ+SVQAAQ Sbjct: 361 FEGGRLRGQLGAASAGYDIEVEHYNALLSQALKGISDQVVTLQSVARQQEQAQQSVQAAQ 420 Query: 421 KNVDIATRAYQRGLTDYLNILHAQTQWLRQQQIVQQLQAQRLAAFAALQTALGGGIEDEA 480 KNVDIATRAYQRGLTDYLN+LHAQTQWLRQQQIVQQLQAQRLAA+AALQTALGGGIE+ A Sbjct: 421 KNVDIATRAYQRGLTDYLNVLHAQTQWLRQQQIVQQLQAQRLAAYAALQTALGGGIEEAA 480 Query: 481 PAKVAAQ 487 PAKVAA+ Sbjct: 481 PAKVAAR 487 >gi|300312564|ref|YP_003776656.1| outer membrane multidrug resistance lipoprotein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 273 bits (699), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 183/470 (38%), Positives = 260/470 (55%), Gaps = 8/470 (1%) Query: 11 ALTVIALSLT---IAGCADQGGIQPQAQLRDVAVLDAGQAIRQAASEAGWPQRAWWQAYG 67 A++V+A L +AGCA + +AQ + + + Q++ +A +A WP WW+ YG Sbjct: 25 AVSVLAAVLAATLMAGCASFSDLGERAQPKSIDRYQSQQSLAASAVQAAWPSDQWWRVYG 84 Query: 68 DAQLNQLVTRAVAGNPSMAVAAARVRQAQSMAVVAHAGELPSAQLDVSVGRKDWSDNVYY 127 DAQLN L+ A+ PSMAVA AR+ +A+ A A P + S+ R S N Sbjct: 85 DAQLNALIDEALQSAPSMAVAKARLMKAEGAAQQQGAALYPQVSANASLDRMKQSYNNGV 144 Query: 128 GAAFRDKLTWNNT--ATLGLSYNVDLWDRNKNATLRAEDEMHAVAADARAAQLPPPPPXL 185 F K +NN ATL SY +D W +N+ A A E+ A ADA A++ Sbjct: 145 PPDFVPK-DYNNATRATLDFSYEIDFWGKNRAALAAATSELEASRADAAQARITLATSIA 203 Query: 186 RTYVQLALQYGLLDNTEAMLKEEQKILDLAQRRFKGGLGTQLEITQAESPLPETRRQMEA 245 Y +LA Y D EA LK + LDL +RF GL T+ + Q E+ + ++A Sbjct: 204 SAYAELAQLYAQRDTNEAALKVRVESLDLFNQRFTNGLETRGSVKQMEARRAIAQADLKA 263 Query: 246 LQENIALIRNQLAALLGAGPGAADGITRPTMQLQTALGLPSALPADLVGRRPDISAARWR 305 E+I L RN+LAALLGAGP +TRP + L LP+ LP +L+GRRPDI AAR R Sbjct: 264 TDESIGLQRNKLAALLGAGPDRGLQLTRPQIDLSRPFALPAQLPVELLGRRPDIVAARLR 323 Query: 306 VEAAAKGISVAKAAFYPNINLLAGIGPAAAGGGLFSFLSAPNLQTTYGPAISLPIFEGGR 365 EAA K I VA+AAFYPN+NL A G + G + + A + + GPAISLPIF GGR Sbjct: 324 AEAAGKQIKVARAAFYPNVNLTAYFGFQSLGIDMLT--RAGSDIGSIGPAISLPIFNGGR 381 Query: 366 LRGQLGAASAGYDIEVEHYNALLSQALKGISDQVVTLQSVARQQKQAQESVQAAQKNVDI 425 LRGQ +ASA YD V +Y+ ++QAL+ ++D V+ +++A + Q + AA++ I Sbjct: 382 LRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKALAGRLADVQAAADAAEEAYRI 441 Query: 426 ATRAYQRGLTDYLNILHAQTQWLRQQQIVQQLQAQRLAAFAALQTALGGG 475 + Y GL YL++L+AQ + + + L+++ AL ALGGG Sbjct: 442 VSNRYNGGLATYLDVLNAQDTLISNLRQLSDLRSRMFTLDVALVRALGGG 491 >gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 495 Score = 236 bits (601), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 167/465 (35%), Positives = 237/465 (50%), Gaps = 17/465 (3%) Query: 22 AGCADQGGIQPQ---AQLRDVAVLDAGQAIRQAASEAGWPQRAWWQAYGDAQLNQLVTRA 78 +GCA GI AQ D A + A WP W + +GDAQL LV +A Sbjct: 27 SGCASFAGIGSDRQVAQAGDFATQRSLSDPNPGAPNGQWPGSDWVRQFGDAQLVALVEQA 86 Query: 79 VAGNPSMAVAAARVRQAQSMAVVAHAGELPSAQLDVSVGRKDWSDNVYYGAAFRDKLTWN 138 + +PS+ A AR+ A ++A A LPS + SV R +S Y + Sbjct: 87 LTSSPSLQQARARIAAASALAESRGAPLLPSVNAEASVTRNQFSSTTIYPPPYGGNWYNE 146 Query: 139 NTATLGLSYNVDLWDRNKNATLRAEDEMHAVAADARAAQLPPPPPXLRTYVQLALQYGLL 198 A L + Y +DLW++N+ A +A A A + A+L + Y QLA QY L Sbjct: 147 KKAGLNVGYELDLWNKNQAALAQAISSEKAAQASEQEARLALTASIVTVYSQLAAQYALH 206 Query: 199 DNTEAMLKEEQKILDLAQRRFKGGLGTQLEITQAESPLPETRRQMEALQENIALIRNQLA 258 D ++ + + + + R + GL +Q+E Q+ + + R Q+ + I L+R QL Sbjct: 207 DILQSTVDQRTSLEKITAERLRTGLDSQIERDQSRTSSADARAQLAQSEGQIVLLRQQLG 266 Query: 259 ALLGAGPGAADGITRPTMQLQTALGLPSALPADLVGRRPDISAARWRVEAAAKGISVAKA 318 AL G GP + P +Q GLP+ LP +L+GRRPDI AARW+VEAA++G+ VAKA Sbjct: 267 ALAGKGPDYGLQLAPPALQGLATPGLPAELPLNLMGRRPDIVAARWQVEAASRGVDVAKA 326 Query: 319 AFYPNINLLAGIGPAAAGGGLFSFL------SAPNLQTTYGPAISLPIFEGGRLRGQLGA 372 FYP+INL A IG F L +A + +GPAI+LPIFEGG LR L Sbjct: 327 RFYPDINLSAMIG--------FDTLLDSNPFTAASKSIAFGPAITLPIFEGGALRAGLKG 378 Query: 373 ASAGYDIEVEHYNALLSQALKGISDQVVTLQSVARQQKQAQESVQAAQKNVDIATRAYQR 432 A Y++ V YN L+ A ++ Q+ + + RQ E++QAA++ +A Y+ Sbjct: 379 EYASYELAVATYNKTLNDAYADVARQIAAIHATERQLPIRSEALQAAERAYALARERYRL 438 Query: 433 GLTDYLNILHAQTQWLRQQQIVQQLQAQRLAAFAALQTALGGGIE 477 GL L +L AQT L Q+Q + LQAQR AL ALGGG + Sbjct: 439 GLVSQLTLLSAQTGVLAQRQAMVALQAQRRDQQVALYKALGGGFD 483 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 156 bits (394), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 139/449 (30%), Positives = 216/449 (48%), Gaps = 22/449 (4%) Query: 53 SEAGW---------PQRAWWQAYGDAQLNQLVTRAVAGNPSMAVAAARVRQAQSMAVVAH 103 SEA W P+ WW+ +GDAQLN L +A+AGN ++A+A AR+ QA++ V Sbjct: 38 SEAPWRSMQPADATPRGPWWERFGDAQLNALQQQALAGNQTLAIATARLAQARAQLNVTG 97 Query: 104 AGELPSAQLDVSVGRKDWSDNV----YYGAAFRDKLTWNNTATLG--LSYNVDLWDRNKN 157 A + P L+ R S N Y F T N LG +SY VD + R + Sbjct: 98 AAQTPQVNLNARAARSRISANRPLTNYNSPNFS---TVQNDFALGASVSYEVDFFGRVQR 154 Query: 158 ATLRAEDEMHAVAADARAAQLPPPPPXLRTYVQLALQYGLLDNTEAMLKEEQKILDLAQR 217 + A AAD +L Y L LD + +++ L+LA Sbjct: 155 SVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELDVELDVVRRAIALQRQALELATA 214 Query: 218 RFKGGLGTQLEITQAESPLPETRRQMEALQENIALIRNQLAALLGAGPGAADGITRPTMQ 277 R G + L++ Q ++ L T Q++ L A + +A L G P A IT P++ Sbjct: 215 RHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEHAIATLTGT-PAPAFSIT-PSLT 272 Query: 278 LQTALGLPSALPADLVGRRPDISAARWRVEAAAKGISVAKAAFYPNINLLAGIGPAAAGG 337 T +P +P+D++ RRPDI+AA + AA I VA AA+YP+ L G + Sbjct: 273 PITPPAIPVGVPSDVLERRPDIAAAERAMAAANAQIGVASAAYYPSFMLQPSYGVDSRNW 332 Query: 338 GLFSFLSAPNLQTTYGPAISLPIFEGGRLRGQLGAASAGYDIEVEHYNALLSQALKGISD 397 + +AP+L + G + S +F+GGRLR + + AGY+ V Y + A++ + D Sbjct: 333 A--TLFNAPSLLWSLGVSASQSLFDGGRLRAGVDFSQAGYEATVASYRRTVLTAMQEVED 390 Query: 398 QVVTLQSVARQQKQAQESVQAAQKNVDIATRAYQRGLTDYLNILHAQTQWLRQQQIVQQL 457 + L ++ R Q+Q ++ +A++ ++IA+ Y+ G T YL+++ AQ L ++ QL Sbjct: 391 GITGLAALERAYAQSQLAIASARRVLEIASSRYEGGATPYLDVITAQQSLLNSERQAAQL 450 Query: 458 QAQRLAAFAALQTALGGGIEDEAPAKVAA 486 QRL L ALGG + P AA Sbjct: 451 MGQRLLVSVFLIKALGGDWQSRTPPAEAA 479 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 143 bits (360), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 129/430 (30%), Positives = 209/430 (48%), Gaps = 31/430 (7%) Query: 62 WWQAYGDAQLNQLVTRAVAGNPSMAVAAARVRQAQSMAVVAHAGELPSAQLDVSVGRKDW 121 WW+ +GD +LN L A N + AAAR+ QA+++ A AG P Q+D VG Sbjct: 78 WWKIFGDEKLNALEDTAQQANQDLKAAAARLGQARALTRDARAGYFP--QVDAGVGATRQ 135 Query: 122 SDNVYYGAAFRDKLTWNNT---ATLGLSYNVDLWDRNKNATLRAEDEMHAVAADARAAQL 178 + D T NT A + +SY VDL+ R + + A ADA+ ++ Sbjct: 136 RPSPASQGLPADADTRPNTLYRAQVNVSYEVDLFGRVAST-------VDAATADAQRSEA 188 Query: 179 PPPPPXLRTYVQLALQYGLLDNTEA-------MLKEEQKILDLAQRRFKGGLGTQLEITQ 231 L +A QY L+ +A + Q+ L L QRRF G +++++ + Sbjct: 189 LFHSVLLALQADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVAR 248 Query: 232 AESPLPETRRQMEALQENIALIRNQLAALLGAGPGAADGITRPTMQLQTALGLPSALPAD 291 A + L + + + A + LA LLG P +P +L A+ +P LP+ Sbjct: 249 ARAELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQPLTRL--AVTVPPGLPSA 306 Query: 292 LVGRRPDISAARWRVEAAAKGISVAKAAFYPNINLLAGIGPAAAG-GGLF-----SFLSA 345 L+ RRPDI+AA + AA + VAK+AF+P++NL G+G +A G LF +FL Sbjct: 307 LLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLG 366 Query: 346 PNLQTTYGPAISLPIFEGGRLRGQLGAASAGYDIEVEHYNALLSQALKGISDQVVTLQSV 405 P + G A+S+P+F+GGR + + A A Y+ +V Y + A + + D + L+ + Sbjct: 367 PLV----GTALSMPLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRIL 422 Query: 406 ARQQKQAQESVQAAQKNVDIATRAYQRGLTDYLNILHAQTQWLRQQQIVQQLQAQRLAAF 465 +Q + E+V A+ + ++ Y+ G Y +++ A L+QQ+ L +R + Sbjct: 423 DQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARST 482 Query: 466 AALQTALGGG 475 L ALGGG Sbjct: 483 VNLIRALGGG 492 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,172,019 Number of extensions: 39279 Number of successful extensions: 278 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 231 Number of HSP's successfully gapped: 18 Length of query: 487 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 398 Effective length of database: 1,198,455 Effective search space: 476985090 Effective search space used: 476985090 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)