BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0343.p (556 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311426|ref|YP_003775518.1| ATP-dependent Clp protease subu... 1061 0.0 gi|300310731|ref|YP_003774823.1| ATP-dependent protease ATP-bind... 309 3e-85 gi|300313318|ref|YP_003777410.1| ClpA/B-type chaperone ATPase [H... 300 2e-82 gi|300310082|ref|YP_003774174.1| ClpA/B-type chaperone protein [... 297 2e-81 >gi|300311426|ref|YP_003775518.1| ATP-dependent Clp protease subunit [Herbaspirillum seropedicae SmR1] Length = 861 Score = 1061 bits (2743), Expect = 0.0, Method: Compositional matrix adjust. Identities = 537/545 (98%), Positives = 540/545 (99%) Query: 1 MRLDKLTTKLQEALADAQSQAVGHDNQYIEPVHLILALLNQDDGGARSLLQRAGVNVNGL 60 MRLDKLTTKLQEALADAQSQAVGHDNQYIEPVHLILALLNQ+DGGARSLLQRAGVNVNGL Sbjct: 1 MRLDKLTTKLQEALADAQSQAVGHDNQYIEPVHLILALLNQEDGGARSLLQRAGVNVNGL 60 Query: 61 STALQPAVKRLPQVSGNGGEVQVGRELVALLNLADKEAQKHGDQFVASEMVLLGLADDKS 120 S ALQ AVKRLPQVSGNGGEVQVGRELVALLNLADKEAQKHGDQFVASEMVLLGL DDKS Sbjct: 61 SAALQAAVKRLPQVSGNGGEVQVGRELVALLNLADKEAQKHGDQFVASEMVLLGLVDDKS 120 Query: 121 DAGRAARENGLTRKSLEAAIKAVRGGASVSSQQDEGQREALKKYTLDLTERARAGKLDPV 180 DAGRAARENGLTRKSLEAAI AVRGGASVSSQQDEGQREALKKYTLDLTERARAGKLDPV Sbjct: 121 DAGRAARENGLTRKSLEAAITAVRGGASVSSQQDEGQREALKKYTLDLTERARAGKLDPV 180 Query: 181 IGRDDEIRRAIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPDSLKSKRVLSL 240 IGRDDEIRRAIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPDSLKSKRVLSL Sbjct: 181 IGRDDEIRRAIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPDSLKSKRVLSL 240 Query: 241 DMAALLAGAKYRGEFEERLKAVLKEIAQDEGQTIVFIDELHTMVGAGKAEGAMDAGNMLK 300 DMAALLAGAKYRGEFEERLKAVLKEIAQDEGQTIVFIDELHTMVGAGKAEGAMDAGNMLK Sbjct: 241 DMAALLAGAKYRGEFEERLKAVLKEIAQDEGQTIVFIDELHTMVGAGKAEGAMDAGNMLK 300 Query: 301 PALARGELHCVGATTLDEYRQYIEKDAALERRFQKILVDEPSVEATIAILRGLQEKYEVH 360 PALARGELHCVGATTLDEYRQYIEKDAALERRFQKILVDEPSVEATIAILRGLQEKYEVH Sbjct: 301 PALARGELHCVGATTLDEYRQYIEKDAALERRFQKILVDEPSVEATIAILRGLQEKYEVH 360 Query: 361 HGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASKIKIEIDSKPEVMDKLDRRLI 420 HGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASKIKIEIDSKPEVMDKLDRRLI Sbjct: 361 HGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASKIKIEIDSKPEVMDKLDRRLI 420 Query: 421 QLKIEREAVKREKDEASQKRLQLIEEEIEKLEREYADLEEIWKAEKSTAQGGQQLKEEIE 480 QLKIEREAVKREKDEASQKRLQLIEEEIEKLEREYADLEEIWKAEKS+AQGGQQLKEEIE Sbjct: 421 QLKIEREAVKREKDEASQKRLQLIEEEIEKLEREYADLEEIWKAEKSSAQGGQQLKEEIE 480 Query: 481 KVRLQMEEATRKSDWQKVSELKYGKLAELEAALEVQNKKDAAGVKAEKPKLVRTQVGAEE 540 KVRLQMEEATRKSDWQKVSELKYGKLAELEAALEVQNKKDAAGVK EKPKLVRTQVGAEE Sbjct: 481 KVRLQMEEATRKSDWQKVSELKYGKLAELEAALEVQNKKDAAGVKTEKPKLVRTQVGAEE 540 Query: 541 IAEIL 545 IAEI+ Sbjct: 541 IAEIV 545 >gi|300310731|ref|YP_003774823.1| ATP-dependent protease ATP-binding specificity subunit [Herbaspirillum seropedicae SmR1] Length = 767 Score = 309 bits (792), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 184/404 (45%), Positives = 252/404 (62%), Gaps = 27/404 (6%) Query: 26 NQYIEPVHLILALLNQDDGGARSLLQRAGVNVNGLSTALQPAVK-RLPQVSGNG---GEV 81 +++I HL+LALL D+ A +L+ VN++ L L + P V G + Sbjct: 22 HEFITVEHLLLALL--DNPSAAEVLRACSVNIDDLRKTLTNFITDNTPTVPGTNEVDTQP 79 Query: 82 QVGRELVALLNLADKEAQKHGDQFVASEMVLLGLADDK-SDAGRAARENGLTRKSLEAAI 140 +G + V + ++ +G + V VL+ + +K S A + G+TR + I Sbjct: 80 TLGFQRVIQRAIMHVQSASNGKKEVTGANVLVAIFGEKDSHAVYYLHQQGVTRLDVVNFI 139 Query: 141 -------------KAVRGGASVSSQQDEGQRE--ALKKYTLDLTERARAGKLDPVIGRDD 185 KA G V Q EGQ++ L ++T +L + A GK+DP+IGR+ Sbjct: 140 SHGVRKDQQADPQKAPEGAEDV---QAEGQQKESPLDQFTQNLNKAAAEGKIDPLIGRES 196 Query: 186 EIRRAIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPDSLKSKRVLSLDMAAL 245 E+ R IQ L RR KNNP+L+GE GVGKTAI EGLA R+ G+VP+ LK+ V SLDM AL Sbjct: 197 EVERVIQTLCRRRKNNPLLVGEAGVGKTAIAEGLAWRVTQGDVPEILKNAVVYSLDMGAL 256 Query: 246 LAGAKYRGEFEERLKAVLKEIAQDEGQTIVFIDELHTMVGAGKAE-GAMDAGNMLKPALA 304 LAG KYRG+FE+RLKAVLK++ +D I+FIDE+HT++GAG A G +DA N+LKPAL+ Sbjct: 257 LAGTKYRGDFEQRLKAVLKQL-KDSPNGILFIDEIHTIIGAGSASGGTLDASNLLKPALS 315 Query: 305 RGELHCVGATTLDEYRQYIEKDAALERRFQKILVDEPSVEATIAILRGLQEKYEVHHGVD 364 G+L C+GATT E+R EKD AL RRFQKI V+EP+VE T+ ILRGL+ K+E HHGV Sbjct: 316 SGQLKCIGATTYTEFRGVFEKDHALSRRFQKIDVNEPTVEQTVQILRGLKSKFEEHHGVK 375 Query: 365 ITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASKIKIEIDSK 408 + A+ +AAEL+ R+I DR LPDKAID+IDEA + +I SK Sbjct: 376 YSASALTSAAELAARFINDRHLPDKAIDVIDEAGAAQRILPKSK 419 >gi|300313318|ref|YP_003777410.1| ClpA/B-type chaperone ATPase [Herbaspirillum seropedicae SmR1] Length = 889 Score = 300 bits (768), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 206/529 (38%), Positives = 288/529 (54%), Gaps = 60/529 (11%) Query: 1 MRLDKLTTKLQE----ALADAQSQAVGHDNQYIEPVHLILALLNQDDGGARSLLQRAGVN 56 + L L KL E A A + V + ++ HL LALL Q + +R+G++ Sbjct: 3 INLRSLICKLNETARLATERAATLCVSCSHYEVDLEHLFLALLEQPQSDFSLIARRSGID 62 Query: 57 VNGLSTALQPAVK-------RLPQVSG-------NGG----------EVQVGRELVALLN 92 +GL L+ + R P +S NG V+ L+ALL Sbjct: 63 TDGLQADLEHEISTFREGNARTPVLSPHLRTLFENGWLIASLEESNPSVRSAHLLLALLT 122 Query: 93 LAD-KEAQKHGDQFVASEMVLLGLADDKSDAGRAARENGLTRKSLEAAI--KAVRGGASV 149 +D + G + A + + K + R LT S+E+A K +GG Sbjct: 123 QSDLSQLAYRGSKLFAK----FKIDEIKHNLHR------LTEGSVESAAIGKTGQGGDRE 172 Query: 150 SS--------QQDEGQREALKKYTLDLTERARAGKLDPVIGRDDEIRRAIQVLQRRSKNN 201 S Q G+ AL +YT LT+RAR GK+DPVIGRD EIR+A+ +L RR +NN Sbjct: 173 SGGDPMGELKQLRIGKMTALDQYTTQLTQRARDGKIDPVIGRDAEIRQAVDILLRRRQNN 232 Query: 202 PVLIGEPGVGKTAIVEGLAQRIVNGEVPDSLKSKRVLSLDMAALLAGAKYRGEFEERLKA 261 P+L GE GVGKTA+VEGLA RI G+VP L+ + +LDM L AGA +GEFE RLK Sbjct: 233 PILTGEAGVGKTAVVEGLALRIAQGDVPKPLQGVEIHTLDMGLLQAGASVKGEFESRLKT 292 Query: 262 VLKEIAQDEGQTIVFIDELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRQ 321 V+ E+ + I+FIDE HTM+GA G DA N+LKPALARGEL + ATT EY++ Sbjct: 293 VIDEVKKSAYPIILFIDEAHTMIGAAGQAGQSDAANLLKPALARGELRTIAATTWSEYKK 352 Query: 322 YIEKDAALERRFQKILVDEPSVEATIAILRGLQEKYEVHHGVDITDPAIVAAAELSHRYI 381 Y EKDAAL RRFQ I V+EP + A+LR + E H GV + D A+V A LSHRYI Sbjct: 353 YFEKDAALARRFQVIKVEEPGEDVACAMLRAMVPLMEQHFGVRVYDEAVVEAVRLSHRYI 412 Query: 382 TDRFLPDKAIDLIDEAASKIKIEIDSKPEVMDKLDRRLIQLKIEREAVKREKDEASQ--K 439 + R LPDKAI ++D A +K+ + D+ P +++ + RRL +L E A++RE SQ + Sbjct: 413 SGRQLPDKAISVLDTACAKVALGRDATPALLEGVTRRLERLDTEISALRREAASGSQHEE 472 Query: 440 RL-QLIEEEIEKLEREYADLEEIWKAEKSTAQGGQQLKEEIEKVRLQME 487 RL L+++ + + E DL+ W+ E+ +L E I++ R +E Sbjct: 473 RLASLLDQHVCAVA-ERHDLQLRWEQER-------ELNERIQQARATLE 513 >gi|300310082|ref|YP_003774174.1| ClpA/B-type chaperone protein [Herbaspirillum seropedicae SmR1] Length = 893 Score = 297 bits (760), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 174/404 (43%), Positives = 245/404 (60%), Gaps = 18/404 (4%) Query: 144 RGGASVSSQQDEGQREALKKYTLDLTERARAGKLDPVIGRDDEIRRAIQVLQRRSKNNPV 203 +GG V + G+ AL +YT +LT+RAR GK+DPV+GRD EIR+A+ +L RR +NNP+ Sbjct: 179 QGGDPVGEMRAGGRTPALDQYTTNLTQRAREGKIDPVVGRDGEIRQAVDILLRRRQNNPI 238 Query: 204 LIGEPGVGKTAIVEGLAQRIVNGEVPDSLKSKRVLSLDMAALLAGAKYRGEFEERLKAVL 263 L GE GVGKTA+VEGLA RI +VP +L+ + +LDM L AGA +GEFE RLK V+ Sbjct: 239 LTGEAGVGKTAVVEGLALRIAQDDVPQALQGVEIHTLDMGLLQAGASVKGEFENRLKGVI 298 Query: 264 KEIAQDEGQTIVFIDELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRQYI 323 E+ + I+FIDE HTM+GAG G DA N+LKPALARGEL + ATT EY++Y Sbjct: 299 DEVKKSPHPIILFIDEAHTMIGAGGQAGQNDAANLLKPALARGELRTIAATTWSEYKKYF 358 Query: 324 EKDAALERRFQKILVDEPSVEATIAILRGLQEKYEVHHGVDITDPAIVAAAELSHRYITD 383 EKDAAL RRFQ I V+EP+ E A+LR + E H GV + D AI A LSHRYI+ Sbjct: 359 EKDAALARRFQVIKVEEPTEEVACAMLRAMAPLMEKHFGVRVYDEAITEAVRLSHRYISG 418 Query: 384 RFLPDKAIDLIDEAASKIKIEIDSKPEVMDKLDRRLIQLKIEREAVKREKDEASQKRLQL 443 R LPDKAI ++D A +K+ + ++ P ++ + RRL +L E A++RE + +L Sbjct: 419 RQLPDKAISVLDTACAKVALGQNATPARLEGVTRRLERLAAEIAALERETRSGASHATRL 478 Query: 444 IEEEIEKLE--REYADLEEIWKAEKSTAQGGQQLKEEIEKVRLQMEEATRKSDWQKVSEL 501 E + + + E +L++ W+ E+ +L I+ R +EEA ++S + S Sbjct: 479 GELQAQHAQAIAEQTELQQRWEQER-------ELNGRIQAARAALEEANQESASSQDSAA 531 Query: 502 KYGKLAELEAALEVQNKKDAAGVKAEKPKLVRTQVGAEEIAEIL 545 +L +L A L+ + E P +V QV +AEI+ Sbjct: 532 ARSELQQLLAQLKEH--------QGETP-MVPVQVDGHVVAEIV 566 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.131 0.349 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,554,469 Number of extensions: 65479 Number of successful extensions: 230 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 227 Number of HSP's successfully gapped: 4 Length of query: 556 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 466 Effective length of database: 1,193,720 Effective search space: 556273520 Effective search space used: 556273520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)