BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0330.p (578 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311435|ref|YP_003775527.1| chloride channel protein [Herba... 995 0.0 gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [... 107 4e-24 gi|300310046|ref|YP_003774138.1| chloride-channel protein [Herba... 72 2e-13 gi|300311782|ref|YP_003775874.1| chloride channel protein [Herba... 70 4e-13 >gi|300311435|ref|YP_003775527.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 555 Score = 995 bits (2573), Expect = 0.0, Method: Compositional matrix adjust. Identities = 523/555 (94%), Positives = 541/555 (97%) Query: 24 MLLWGALAGFVGALATIAFRECIALLQIWLTGHDGSFVDIAKGLSWPVRVLLPTCGGVIA 83 MLLWGALAGFVGALATIAFRECIALLQIW+TGHDGSFVDIAKGL W RVLLPTCGGV+A Sbjct: 1 MLLWGALAGFVGALATIAFRECIALLQIWMTGHDGSFVDIAKGLPWYTRVLLPTCGGVVA 60 Query: 84 GLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGSIGREGPMVQL 143 GLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGSIGREGPMVQL Sbjct: 61 GLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGSIGREGPMVQL 120 Query: 144 SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIVLGSLVMESFGP 203 SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIVLGSLVMESFGP Sbjct: 121 SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIVLGSLVMESFGP 180 Query: 204 VVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVLLFLVLGVLAGILAPQFLRVLELGK 263 VVVASVVANITMRELPGYRAAYEMPYFPEIGGWEV+LFLVLGVLAG+LAPQFLRVLELGK Sbjct: 181 VVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVMLFLVLGVLAGVLAPQFLRVLELGK 240 Query: 264 QGFGKFKLPLPVRLGVGGLLVGLISIEVPEVWGNGYSLVNSLLHTTWLWQAVLMVLVIKV 323 GFGK +LPLPVRLGVGGLLVGLIS++VPEVWGNGYSLVNSLLHT+W+WQAVLMVLV+KV Sbjct: 241 HGFGKLRLPLPVRLGVGGLLVGLISVQVPEVWGNGYSLVNSLLHTSWVWQAVLMVLVVKV 300 Query: 324 LATAITVGSGAVGGIFTPTLFVGAAVGYLFGDTAQALLPFHMSQPFAYAMVGMGAFLAAA 383 LAT+ITVGSGAVGGIFTPTLFVGAAVGYLFGD AQALLP H+SQPFAYAMVGMGAFLAAA Sbjct: 301 LATSITVGSGAVGGIFTPTLFVGAAVGYLFGDAAQALLPVHISQPFAYAMVGMGAFLAAA 360 Query: 384 SYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARSTDGRSMYEITLKRHRDNEERLRL 443 SYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARS DGRSMYEITLKRHRDNEERLRL Sbjct: 361 SYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARSVDGRSMYEITLKRHRDNEERLRL 420 Query: 444 RGTHMSDLIRPADTVLSETASLEEVSALFLKYPVKYVYIVDAHERYQGVVALQDITSWLL 503 RGTHMS+LIRPA+TVL E ASLEE+SALFLKYPVKYVYIVD +RY GVVALQDITSWLL Sbjct: 421 RGTHMSELIRPAETVLHEEASLEEISALFLKYPVKYVYIVDGRQRYLGVVALQDITSWLL 480 Query: 504 DKKEPAGRVARDFLRPHFLHEVTPDMSLGEALQLFLDHQGERLPVIASAEDPRLLGVVFK 563 DKKE AGRVARDFLRPHFLHEVTPDMSLGEALQLFLDHQGERLPVIASA+DP+LLGVVFK Sbjct: 481 DKKEAAGRVARDFLRPHFLHEVTPDMSLGEALQLFLDHQGERLPVIASADDPQLLGVVFK 540 Query: 564 TSLLDAYFRLDRAGN 578 TSLLDAYFRLDRAGN Sbjct: 541 TSLLDAYFRLDRAGN 555 >gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [Herbaspirillum seropedicae SmR1] Length = 572 Score = 107 bits (266), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 147/518 (28%), Positives = 229/518 (44%), Gaps = 39/518 (7%) Query: 67 LSWPVRVLLPTCGGVIAGLFLVWAKRTPAGNG-GDYMEAVAIGDGAIPVRNTLLRSISSL 125 L W + LLP GG+I GL + G+G + +EA+ G + + LL+ +SS Sbjct: 62 LGW-IAALLPIAGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSG 120 Query: 126 ASIASGGSIGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGA 185 I SGG G EGP++ SL+ + H ++ + L+ GA AG+T+ + P+A Sbjct: 121 IVIGSGGPFGAEGPIIMTGGSIGSLLAQLLHLTAAERKTLLVAGACAGMTAIFGTPLAAV 180 Query: 186 FFITEIVLGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFP-EIGG---WEVLLF 241 E++L L S PV +A VA L P FP ++ +L Sbjct: 181 LLAVELLLFELKPRSLLPVALACAVAGFVRPWL-----VEAGPLFPLDVAAPTPVALLSC 235 Query: 242 LVLGVLAGILAPQFLRVLELGKQGFGKFKLPLPVRLGVGGLLVGLISIEVPEVWGNGYSL 301 LV G+ +G+LA L + GF + +L +GG+ VGL + P G GY + Sbjct: 236 LVAGLASGVLAAALTLALYRIEDGFHRLRLHWMWWPALGGIAVGLGGLLQPRALGVGYDV 295 Query: 302 VNSLLHTTWLWQAVLMVLVIKVLATAITVGSGAVGGIFTPTLFVGAAVGYLFGDTAQALL 361 + LLH L A L +L++K + + +GSG GG+ P L +GA +G L Sbjct: 296 IGDLLHQHMLLSAALALLLVKAVIWLLALGSGTSGGVLAPLLMLGAGLGL----VLSPWL 351 Query: 362 PFHMSQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAY-----V 416 P + +V M L + APL AI+ F +T + Q +LPL+ + VAY + Sbjct: 352 PG--GSDGLWPLVCMAGVLGSVLGAPLTAIVFAFGLTHASQALLPLIATTAVAYGVNVLL 409 Query: 417 VARSTDGRSMYEITLKRHR----DNEERLRLRGTHMSDLIRPA-DTVLSETASLEEVSAL 471 + RS + L HR D ER+ H+ +L+ A T+ + E ++ Sbjct: 410 MKRSIMTEKIARRGLHVHREYGVDPLERM-----HVEELMTAAPQTLAGALPAAEALAWC 464 Query: 472 FLKYPVKYVYIVDAHERYQGVVALQDITSWLLDKKEPAGRVARDFLRPHFLHEVTPDMSL 531 + V Y V G++AL+ + D +P D L H + P MS Sbjct: 465 RAQEHVHRYYPVTDGVHLVGMLALRALEH--ADASQPC-----DTLAQGHEHYLLPHMSA 517 Query: 532 GEALQLFLDHQGERLPVIASAEDPRLLGVVFKTSLLDA 569 A + RLPV+A E +L+GV+ + L A Sbjct: 518 RSAAGMMARLGVARLPVVAGPERRKLVGVLSLSDLARA 555 >gi|300310046|ref|YP_003774138.1| chloride-channel protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 71.6 bits (174), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 105/375 (28%), Positives = 166/375 (44%), Gaps = 41/375 (10%) Query: 88 VWAKRT--PAGNGGDYMEAVAIGDGAIPVRNTLLRSIS--------SLASIASGGSIGRE 137 VW R P G +A+A G R + L S+ S+ +IA G +IGRE Sbjct: 83 VWLTRRYFPGSEGSGIPQAIATLHGDTAERGSTLLSLRVLLGKIVLSVVAIAGGMTIGRE 142 Query: 138 GPMVQL-SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIVLGSL 196 GP VQ+ +A+ +L + R L+ GAAAG+ +A+N P+AG F E + S Sbjct: 143 GPTVQIGAAIMYNLRRLYPAGYEHMERRLILAGAAAGLAAAFNTPLAGIVFAIEELSRSF 202 Query: 197 VMESFGPVVVASVVANITMRELPGYRAAYEMPYFP-EIGGWEVLLFLVLGVLAGILAPQF 255 + G V+ + A + + G + FP E G +L +V VL G+ F Sbjct: 203 EQRASGVVITTIIFAGLVALSINGNYTYFGSIAFPGETSGRLILAVIVTAVLMGVAGGLF 262 Query: 256 ----LRVLELGKQGFGKFKLPLPVRL-GVGGLLVGLISIEV-PEVWGNGYSLVNSLLHTT 309 L + PV V G +V +I + +G+GY + LL+ Sbjct: 263 CWLILNPTRWIPPALVTLRTERPVAFAAVCGFIVAVIGVAAGGATFGSGYEQAHGLLN-- 320 Query: 310 WLWQAVLMVLVIKVLATAITVGS---GAVGGIFTPTLFVGAAVG----YLFGDTAQALLP 362 + + L A +GS G GGIF P+L +GA G LFG T+ +L Sbjct: 321 ---DGQGLSVFYPFLKFASMIGSYLPGLPGGIFAPSLSIGAGFGNLLHLLFGGTSLPML- 376 Query: 363 FHMSQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARSTD 422 A AMVG +LAA + +P+ A +++ EM + +V+ LM + ++A V++ Sbjct: 377 ------VALAMVG---YLAAVTQSPITAFVIVMEMVDGHALVISLMATALIASAVSK-VF 426 Query: 423 GRSMYEITLKRHRDN 437 +YE +R+ + Sbjct: 427 APPLYETLAERYLKS 441 >gi|300311782|ref|YP_003775874.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 70.1 bits (170), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 94/358 (26%), Positives = 148/358 (41%), Gaps = 30/358 (8%) Query: 74 LLPTCGGVIAGLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGS 133 LLP G + L+L K AGN ++ + IP+R L + ++AS G S Sbjct: 57 LLPLGGFAVGWLYLHTGKPVEAGNN-LLIDEIHDPRKVIPLRMAPLVLLGTVASHLFGAS 115 Query: 134 IGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIV- 192 +GREG VQ+ A + R+L+ G +AG +S + P+AGA F E++ Sbjct: 116 VGREGTAVQMGGALADQLTHLLRLRPEDRRILLMAGISAGFSSVFGTPMAGAIFGLEVLA 175 Query: 193 LGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVLLFLVLGVLAGILA 252 +G + E+ P VA++ A+ + Y M + W V + GV+ G+ Sbjct: 176 IGRMRYEAIFPCFVAAIAADQVGLAWGVHHTHYAMNASAPMALWSVAAVIAAGVVFGLAG 235 Query: 253 PQFLRVLELGKQGFGKFKLPLPVRLGVGGLLVGLISIEVPEVWGNGYS---------LVN 303 F + P+R +GG +V L VW G +V Sbjct: 236 MIFANSTHTLSAFMKRHVSYAPLRPFIGGFIVAL------AVWAVGTHRYIGLGIPVIVE 289 Query: 304 SLLHTTWLWQAVLMVLVIKVLATAITVGSGAVGGIFTPTLFVGAAVGYLFGDTAQALLPF 363 + W + K++ T ++GSG GG TP ++GA +G AL P Sbjct: 290 AFQQPLAPWD-----FLGKMVFTVTSLGSGFKGGEVTPLFYIGATLG-------NALAPL 337 Query: 364 HMSQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARST 421 A +G A A A+ PL + +M E+ Q+ L+CVVAY+ + T Sbjct: 338 LHLPFPLLAGIGFVAVFAGAANTPLASTIMAIEL-FGPQIGPFAALACVVAYLFSGHT 394 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.140 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,709,051 Number of extensions: 69171 Number of successful extensions: 306 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 294 Number of HSP's successfully gapped: 4 Length of query: 578 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 488 Effective length of database: 1,193,720 Effective search space: 582535360 Effective search space used: 582535360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)