BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0036.0319.p
         (507 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311444|ref|YP_003775536.1| bifunctional (P)ppGpp synthetas...   826   0.0  
gi|300311292|ref|YP_003775384.1| (p)ppGpp synthetase I [Herbaspi...   252   6e-68

>gi|300311444|ref|YP_003775536.1| bifunctional (P)ppGpp synthetase
           II/guanosine-3',5'-bis pyrophosphate
           3'-pyrophosphohydrolase [Herbaspirillum seropedicae
           SmR1]
          Length = 753

 Score =  826 bits (2134), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 401/404 (99%), Positives = 403/404 (99%)

Query: 1   VATFAHLIPKLEEYLTPSELKKVKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWK 60
           VATFAHLIPKLEEYLTPSELKKVKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWK
Sbjct: 39  VATFAHLIPKLEEYLTPSELKKVKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWK 98

Query: 61  LDAQAMMAAFLHDVMEDQGVKKEELIERFGASVASLVDGLSKLDKIEFQSQVEAQAENFR 120
           LDAQAMMAAFLHDVMEDQGVKKEELIERFGASVASLVDGLSKLDKIEFQSQVEAQAENFR
Sbjct: 99  LDAQAMMAAFLHDVMEDQGVKKEELIERFGASVASLVDGLSKLDKIEFQSQVEAQAENFR 158

Query: 121 KMLLAMARDVRVILVKLADRLHNMRTLGSMPPEKKRRISRETMEVYVPIAHRLGLNNIYR 180
           KMLLAMARDVRVILVKLADRLHNMRTLGSMPPEKKRRISRETMEVYVPIAHRLGLNNIYR
Sbjct: 159 KMLLAMARDVRVILVKLADRLHNMRTLGSMPPEKKRRISRETMEVYVPIAHRLGLNNIYR 218

Query: 181 ELQELSFAHLHPLRHRTLLKAVKAARGNRREVVTKIMESVTSTLIAAGIPAQVDGREKTL 240
           ELQELSFAHLHPLRHRTL KAVKAARGNRREVVTKIMESVTSTLIAAGIPAQVDGREKTL
Sbjct: 219 ELQELSFAHLHPLRHRTLSKAVKAARGNRREVVTKIMESVTSTLIAAGIPAQVDGREKTL 278

Query: 241 YGIYRKMRNKHLSFSQVLDVYGFRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIAIPKL 300
           YGIYRKMRNKHLSFSQVLDVYGFRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIAIPKL
Sbjct: 279 YGIYRKMRNKHLSFSQVLDVYGFRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIAIPKL 338

Query: 301 NGYQSLHTTLIGPYGTPVEFQIRTTDMHRVAESGVAAHWLYKDDEGSLTDLQQRTHAWLQ 360
           NGYQSLHTTLIGPYGTPVEFQIRT++MHRVAESGVAAHWLYKDDEGSLTDLQQRTHAWLQ
Sbjct: 339 NGYQSLHTTLIGPYGTPVEFQIRTSEMHRVAESGVAAHWLYKDDEGSLTDLQQRTHAWLQ 398

Query: 361 SLLDIQKQTGDSAEFLEHVKVDLFPDSVYVFTPKSKIIALPRGA 404
           SLLDIQKQTGDSAEFLEHVKVDLFPDSVYVFTPKSKIIALPRGA
Sbjct: 399 SLLDIQKQTGDSAEFLEHVKVDLFPDSVYVFTPKSKIIALPRGA 442


>gi|300311292|ref|YP_003775384.1| (p)ppGpp synthetase I
           [Herbaspirillum seropedicae SmR1]
          Length = 749

 Score =  252 bits (643), Expect = 6e-68,   Method: Compositional matrix adjust.
 Identities = 149/420 (35%), Positives = 227/420 (54%), Gaps = 28/420 (6%)

Query: 15  LTPSELKKVKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWKLDAQAMMAAFLHDV 74
           L P +  +V +A    + ++  +   SG+  +   ++VA +     +DA   +AA   + 
Sbjct: 19  LGPEDAARVAQALAELEPLYADRQILSGQNALQFSISVATVLTALNVDAATRIAALAFEA 78

Query: 75  MEDQGVKKEELIERFGASVASLVDGLSKLDKI---EFQSQVEA------------QAENF 119
                  +E+L ERFG  +A LV  + +L +     FQ Q E             Q E  
Sbjct: 79  QMLHPELEEKLEERFGKEIADLVGNVRQLMRFHSFSFQPQQEVLRGKNAAQQAAAQIETL 138

Query: 120 RKMLLAMARDVRVILVKLADRLHNMRTLGSMPPEKKR--RISRETMEVYVPIAHRLGLNN 177
           RKMLLAMA D+R +LV+LA R+  +R       E +     +R+T E+Y P+A+RLG+  
Sbjct: 139 RKMLLAMATDMRAVLVRLASRVATLRYFAETKQEDEASANYARQTFELYAPLANRLGIWQ 198

Query: 178 IYRELQELSFAHLHPLRHRTLLKAVKAARGNRREVVTKIMESVTSTLIAAGIPAQVDGRE 237
           +  EL++LSF  L P+ ++ + K ++  R  R   V   +  + + + A  I A+V GR 
Sbjct: 199 LKWELEDLSFRFLEPVTYKRIAKMLEEKRLEREAFVANAIARLRAEMAAQDIRAEVSGRP 258

Query: 238 KTLYGIYRKMRNKHLSFSQVLDVYGFRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIAI 297
           K +Y I+ KMR KH+ FS++ DV  FRV+VD    CY  LG +H ++ P+P +F DYI+ 
Sbjct: 259 KHIYSIWNKMRGKHIDFSELYDVRAFRVIVDDVKTCYAVLGIVHDIWTPIPEEFDDYISR 318

Query: 298 PKLNGYQSLHTTLIGPYGTPVEFQIRTTDMHRVAESGVAAHWLYKDDEGSLTDLQQRTH- 356
           PK NGYQSLHT +    G  +E QIRT +MH  AE GVAAHW YK+  GS    Q+    
Sbjct: 319 PKQNGYQSLHTVVTAEDGRALEVQIRTHEMHHFAEYGVAAHWRYKESGGSNFSAQKYDEK 378

Query: 357 -AWLQSLLDIQKQTGDSA--------EFLEHVKVDLFPDSVYVFTPKSKIIALPRGA-PI 406
            AWL+ LL  + +  D+         +++E +K     + +YV TP++++I LP GA PI
Sbjct: 379 IAWLRQLLAWKNEVTDAVVDQEEVRRDWVEKLKSATLDERIYVLTPQARVIELPSGATPI 438


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.136    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,481,395
Number of extensions: 59043
Number of successful extensions: 178
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 175
Number of HSP's successfully gapped: 2
Length of query: 507
Length of database: 1,619,870
Length adjustment: 89
Effective length of query: 418
Effective length of database: 1,198,455
Effective search space: 500954190
Effective search space used: 500954190
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 133 (55.8 bits)