BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0309.p (692 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311453|ref|YP_003775545.1| signal transduction containing ... 1242 0.0 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 431 e-122 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 421 e-118 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 414 e-117 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 380 e-106 >gi|300311453|ref|YP_003775545.1| signal transduction containing PAS/GGDEF/EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 683 Score = 1242 bits (3213), Expect = 0.0, Method: Compositional matrix adjust. Identities = 614/684 (89%), Positives = 642/684 (93%), Gaps = 1/684 (0%) Query: 3 MISSVGTDDLACCSALLDAMQNLQGMALHAALGGIASGFLKRPGGRYVVSPPPESASGLC 62 M+SSVGTDDLACCSALL+AMQNL GMALHAALGGIASG LKRPGGRYV+ GLC Sbjct: 1 MLSSVGTDDLACCSALLEAMQNLHGMALHAALGGIASGLLKRPGGRYVIGASAPVEGGLC 60 Query: 63 QVVEHEGARFGHFVFPALTRPYTELERTRLAMLAAFLGRLMQRRADTDKRARSLDQIEAK 122 Q V+ EGA FGHFVFP LTRPY++ ER RLAMLAAFLGRLMQ RADT++R R+LDQIEAK Sbjct: 61 QPVQCEGALFGHFVFPPLTRPYSDQERQRLAMLAAFLGRLMQGRADTEQRVRALDQIEAK 120 Query: 123 LEQQAQILNQMHESVITMDPAGFITSWNRGAEQLFGYSALEAIGRNVLFLYENEEEEDSL 182 LEQQAQILNQMHESVITMDPAGFITSWNRGAEQLFGYSA+EAIGRNVLFLYENE+EEDSL Sbjct: 121 LEQQAQILNQMHESVITMDPAGFITSWNRGAEQLFGYSAMEAIGRNVLFLYENEDEEDSL 180 Query: 183 HDLFLEQGGREMEVRRRKKSGEVFWASLSLSVMRDQHGHPIGLIGYLNDITERKNAEKLI 242 HDLFLE GGREMEVRRRKKSGEVFWASLSLSVMRDQ+GHPIGLIGYLNDITERKNAEKLI Sbjct: 181 HDLFLEHGGREMEVRRRKKSGEVFWASLSLSVMRDQNGHPIGLIGYLNDITERKNAEKLI 240 Query: 243 HHLAYYDALTGLPNRTLLTRTVDQALTEARHENMHGCVMFIDLNRFKPINDTLGHVAGDM 302 HHLAYYDALTGLPNRTLLTRTVDQAL EARHEN+HGCVMFIDLNRFKPINDTLGHVAGDM Sbjct: 241 HHLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMFIDLNRFKPINDTLGHVAGDM 300 Query: 303 LLVEVAIRLRRALREQDVVARLGADEFAVALFDIDKDYHPGFVAQKLLAVFDDPFFIDGH 362 LLVEVA+RLRRALREQDVVARLGADEFAVALF IDKD P +VAQKLLA+FDDPFFIDGH Sbjct: 301 LLVEVAMRLRRALREQDVVARLGADEFAVALFGIDKD-SPAYVAQKLLALFDDPFFIDGH 359 Query: 363 ELRVGASIGVSMYPEDATDTETLLRLADIAMYRAKQGGDNNENGYVYYSEEMNRNTLDLL 422 ELRVGASIGVS+YPE+ATDTETLLRLADIAMYRAKQGG+NNE GY YSEEMNRNTLDLL Sbjct: 360 ELRVGASIGVSLYPENATDTETLLRLADIAMYRAKQGGENNEGGYACYSEEMNRNTLDLL 419 Query: 423 RIETGLLHAFEYNELLLYYQPKVDMNTLAITGGEALVRWQHPERGLLLPGEFIPIAEETG 482 RIETGLLHAFEYNELLLYYQPKVDM TLAITG EALVRWQHPERGLLLPGEFIPIAEETG Sbjct: 420 RIETGLLHAFEYNELLLYYQPKVDMQTLAITGAEALVRWQHPERGLLLPGEFIPIAEETG 479 Query: 483 LIVQLSDWVLDAVCRQARSWRDAGLAPIRIALNVTAREFTRSLPDRVRAALNRHSLSGDW 542 LIVQLSDWVLDAVCRQAR+W+DAGL PIRIALNVTAREFTRSLPDRV AAL RH LSG+W Sbjct: 480 LIVQLSDWVLDAVCRQARAWKDAGLPPIRIALNVTAREFTRSLPDRVGAALERHMLSGEW 539 Query: 543 LELEITESMLMHSTDRVISIMEKICSLGITISLDDFGTGYSSLSYLKRFPINTLKIDRSF 602 LELEITESMLMHSTDRVISIMEKIC LGIT+SLDDFGTGYSSLSYLKRFPINTLKIDRSF Sbjct: 540 LELEITESMLMHSTDRVISIMEKICGLGITVSLDDFGTGYSSLSYLKRFPINTLKIDRSF 599 Query: 603 TMGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVETQKQFNFLREAGCNELQGYIFSRPIP 662 TMGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVE +KQFNFLREAGCNELQGYIFSRP+P Sbjct: 600 TMGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVEDEKQFNFLREAGCNELQGYIFSRPVP 659 Query: 663 AVDFHAMLLEGRKLNFVPETSAPA 686 A DFHAMLLEGRKL +++ A Sbjct: 660 APDFHAMLLEGRKLALESASASQA 683 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 431 bits (1109), Expect = e-122, Method: Compositional matrix adjust. Identities = 236/575 (41%), Positives = 344/575 (59%), Gaps = 12/575 (2%) Query: 110 DKRARSLDQIEAKLEQQAQILNQMHESVITM---DPAGFITSWNRGAEQLFGYSALEAIG 166 D +AR L + +L QA + +++ + +P I N ++ GYS+ EA+G Sbjct: 379 DIKARRLAERALRLRHQA--IEASANAILILSAQEPDYTIEYVNPAFARITGYSSTEAVG 436 Query: 167 RNVLFLYENEEEEDSLHDLFL---EQGGREMEVRRRKKSGEVFWASLSLSVMRDQHGHPI 223 +++ FL + + + +++ EQ + +R K G W ++ +RD+ G Sbjct: 437 KSIRFLEGRDSDPEGFETVWIAMREQREHNVVLRNYHKDGSELWNDFYIAPVRDESGEVT 496 Query: 224 GLIGYLNDITERKNAEKLIHHLAYYDALTGLPNRTLLTRTVDQALTEARHENMHGCVMFI 283 + L DIT K E + A D LTGL NR +L ++QA+ A + V+F+ Sbjct: 497 HFVAALYDITSMKRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFV 556 Query: 284 DLNRFKPINDTLGHVAGDMLLVEVAIRLRRALREQDVVARLGADEFAVALFDIDKDYHPG 343 DL+RFK +NDTLGH AGD LL VA RLR A+RE D VARLG DEF + L + D+ Sbjct: 557 DLDRFKFVNDTLGHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFIMILPEQDELTLDT 616 Query: 344 FVAQKLLAVFDDPFFIDGHELRVGASIGVSMYPEDATDTETLLRLADIAMYRAKQGGDNN 403 Q+++ P + GH L + S+GV+ YP D TD ETL++ ADIAMYR KQ G NN Sbjct: 617 RSLQRIMDAVAAPVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNN 676 Query: 404 ENGYVYYSEEMNRNTLDLLRIETGLLHAFEYNELLLYYQPKVDMNTLAITGGEALVRWQH 463 + +Y+ MN L+ LRIE L +A ++ E LL+YQPKVD+ + + G EAL+RWQH Sbjct: 677 ---FQFYTPAMNEQALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQH 733 Query: 464 PERGLLLPGEFIPIAEETGLIVQLSDWVLDAVCRQARSWRDAGLAPIRIALNVTAREF-T 522 P+ G++ P FI +AEETGLI+ + WVL A C Q R W+ G A I +++N++ +F Sbjct: 734 PQLGMVSPARFIGLAEETGLILPIGAWVLRAACTQCRQWQLGGYADISVSVNLSVGQFLQ 793 Query: 523 RSLPDRVRAALNRHSLSGDWLELEITESMLMHSTDRVISIMEKICSLGITISLDDFGTGY 582 + L + L L + LELE+TES++M + I I+ + LG+ +S+DDFGTGY Sbjct: 794 KDLVSSIADVLEDTGLPPESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGY 853 Query: 583 SSLSYLKRFPINTLKIDRSFTMGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVETQKQFN 642 SSL+YLKRFPI+ LKIDRSF I D +D AI ++II +A+ L+ +VIAEGVET +Q + Sbjct: 854 SSLAYLKRFPIDVLKIDRSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQLD 913 Query: 643 FLREAGCNELQGYIFSRPIPAVDFHAMLLEGRKLN 677 +LR GC+E+QGY+FS P+PA F +L + R + Sbjct: 914 YLRHYGCDEIQGYLFSPPVPADKFEQILRQDRVMT 948 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 421 bits (1081), Expect = e-118, Method: Compositional matrix adjust. Identities = 219/443 (49%), Positives = 291/443 (65%), Gaps = 5/443 (1%) Query: 234 ERKNAEKLIHHLAYYDALTGLPNRTLLTRTVDQALTEARHENMHGCVMFIDLNRFKPIND 293 E + A++ I HLA+YD LTGLPNR L T+ V A+T A H N V+ +DL+RFK IND Sbjct: 658 ESRRADERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVILMDLDRFKNIND 717 Query: 294 TLGHVAGDMLLVEVAIRLRRALREQDVVARLGADEFAVALFDIDKDYHPGFVAQKLLAVF 353 T GH G+ +L E+A R R +LRE D++AR+G DEF V + D D G +AQKLL Sbjct: 718 TFGHETGEAVLQEIAQRFRNSLRELDILARVGGDEFIVLVDDFDDPVQLGEIAQKLLTEA 777 Query: 354 DDPFFIDGHELRVGASIGVSMYPEDATDTETLLRLADIAMYRAKQGGDNNENGYVYYSEE 413 PF IDG E + SIG++ YP D + +TL++ ADIAMYRAK G ++ Y ++S+E Sbjct: 778 RKPFIIDGQEAMLSVSIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDD---YRFFSDE 834 Query: 414 MNRNTLDLLRIETGLLHAFEYNELLLYYQPKVDMNTLAITGGEALVRWQHPERGLLLPGE 473 +N NT++ + +E L A E E +++YQPK+++ I G EALVRWQHP RGLL PGE Sbjct: 835 INTNTVERIALEAELRRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPGE 894 Query: 474 FIPIAEETGLIVQLSDWVLDAVCRQARSWRDAGLAPIRIALNVTAREF-TRSLPDRVRAA 532 FI +AEET LI Q+ VLD CR R + RI++N+T +F L D ++ Sbjct: 895 FITLAEETRLIDQIGLLVLDVACRDIGLMRQQQMTCGRISINLTGSQFGDERLLDDIKEV 954 Query: 533 LNRHSLSGDWLELEITESMLMHSTDRVISIMEKICSLGITISLDDFGTGYSSLSYLKRFP 592 ++RH LE EITESM+MH+ +R I+IM+ I + G TIS+DDFGTGYSSL+YLKRFP Sbjct: 955 VDRHGTPPSCLEFEITESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRFP 1014 Query: 593 INTLKIDRSFTMGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVETQKQFNFLREAGCNEL 652 +NTLKID+SF IPD+ N AI AII ++ L KV+ EGVET+KQ LRE G +E Sbjct: 1015 VNTLKIDKSFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDEY 1074 Query: 653 QGYIFSRPIPAVDFHAMLLEGRK 675 QGY FS+P+P F LL G++ Sbjct: 1075 QGYYFSKPVPYAQF-VQLLAGQQ 1096 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 414 bits (1065), Expect = e-117, Method: Compositional matrix adjust. Identities = 231/571 (40%), Positives = 331/571 (57%), Gaps = 9/571 (1%) Query: 108 DTDKRARSLDQIEAKLEQQAQILNQMHESVITMDPAGFITSWNRGAEQLFGYSALE--AI 165 D +R + ++ + I E V P G + N +++GY + E + Sbjct: 123 DVTERKEAAQALQEAERRYRSIFENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDLIV 182 Query: 166 G-RNVLF-LYENEEEEDSLHDLFLEQGG-REMEVRRRKKSGEVFWASLSLSVMRDQHGHP 222 G R++ LY E L EQG E R ++ GE+ W S + + D+ G Sbjct: 183 GLRDISHQLYVEPERRQEFMRLMEEQGSVSNFESRVYRRDGEIIWISENARAVYDEAGKL 242 Query: 223 IGLIGYLNDITERKNAEKLIHHLAYYDALTGLPNRTLLTRTVDQALTEARHENMHGCVMF 282 G + ITERK E + H A +DALTGLPNR +L + +AL AR + V F Sbjct: 243 SCYEGTVEAITERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTAVAF 302 Query: 283 IDLNRFKPINDTLGHVAGDMLLVEVAIRLRRALREQDVVARLGADEFAVALFD-IDKDYH 341 +DL++FK IND+LGH GD LL VA RL+ LRE D+VAR G DEF + L + + + Sbjct: 303 VDLDQFKFINDSLGHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEAG 362 Query: 342 PGFVAQKLLAVFDDPFFIDGHELRVGASIGVSMYPEDATDTETLLRLADIAMYRAKQGGD 401 V Q++LA P+ E +V ASIGVS YP D D E+LL+ AD AMYRAK+ G Sbjct: 363 IAEVMQRILAAVARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGR 422 Query: 402 NNENGYVYYSEEMNRNTLDLLRIETGLLHAFEYNELLLYYQPKVDMNTLAITGGEALVRW 461 NN + +++ M+ + L + L A + +E LYYQPK+ + + G EAL+RW Sbjct: 423 NN---FQFFAPWMDSQVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRW 479 Query: 462 QHPERGLLLPGEFIPIAEETGLIVQLSDWVLDAVCRQARSWRDAGLAPIRIALNVTAREF 521 + P++G++ P FIP AEE GLIV + +WVL CRQ ++W+ AGL PI +A+N++ R+ Sbjct: 480 ESPQQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQL 539 Query: 522 TRSLPDRVRAALNRHSLSGDWLELEITESMLMHSTDRVISIMEKICSLGITISLDDFGTG 581 +SLPD V L + L+ WLELEITE+++M ++ ++ + + LG+ IS+DDFGTG Sbjct: 540 NQSLPDFVAGVLAQSGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTG 599 Query: 582 YSSLSYLKRFPINTLKIDRSFTMGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVETQKQF 641 YSSLSYL+RFP++ LKID+SF I D + AI AII +A L +VIAEGVE ++Q Sbjct: 600 YSSLSYLRRFPVDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQH 659 Query: 642 NFLREAGCNELQGYIFSRPIPAVDFHAMLLE 672 FL+E C+E+QGY F +P+ F A + E Sbjct: 660 AFLKENACDEVQGYYFGKPMEVAVFTAWMSE 690 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 380 bits (976), Expect = e-106, Method: Compositional matrix adjust. Identities = 233/603 (38%), Positives = 343/603 (56%), Gaps = 37/603 (6%) Query: 98 FLGRLMQRRADTDKRARSLDQIEAKLEQQAQILNQMHESVITMDPAGFITSWNRGAEQLF 157 F G R TD++ QIEA+L A + E ++ D I N+ ++ Sbjct: 454 FTGFRTSSRDITDRK-----QIEAELRISAVAFDS-REGMLITDANSKILRVNKAFTEIT 507 Query: 158 GYSALEAIGRNVLFLYEN-------EEEEDSLHDLFLEQGGREMEVRRRKKSGEVFWASL 210 GYSA E +G++ L + +E +S+ QG E+ R+K+GEV+ L Sbjct: 508 GYSAEEIVGKHPNLLRSDRHGPAFFQEMRESIRRFGKWQG----EIWDRRKNGEVYPEWL 563 Query: 211 SLSVMRDQHGHPIGLIGYLNDITERKNAEKLIHHLAYYDALTGLPNRTLLTRTVDQALTE 270 ++S ++++ + +DI++RK AE+ I LA++DALT LPNRTLL + QA+ Sbjct: 564 TISAVKNKDQQVTHYVSTHHDISDRKLAEERIRELAFFDALTRLPNRTLLLDRLKQAIAL 623 Query: 271 ARHENMHGCVMFIDLNRFKPINDTLGHVAGDMLLVEVAIRLRRALREQDVVARLGADEFA 330 + ++FIDL+ FK +NDT+GH GD LL +VA RL +RE D VAR+G DEF Sbjct: 624 SLRTKTCAALLFIDLDHFKTLNDTIGHEKGDTLLKQVAQRLLANVRENDTVARVGGDEFV 683 Query: 331 VALFDIDKDYHPGF-----VAQKLLAVFDDPFFIDGHELRVGASIGVSMYPEDATDTETL 385 V L +++ + +K+L + +D E R ASIG +++ A + L Sbjct: 684 VVLEGLNELQQEAATQTRAIGEKILTALGSTYQLDDVEYRTTASIGATVFLGRAASVDEL 743 Query: 386 LRLADIAMYRAKQGGDNNENGYVYYSEEMNRNTLDLLRIETGLLHAFEYNELLLYYQPKV 445 ++ AD+AMY+AK+ G N ++ M L+ +E GL A + N+LLL+YQP+V Sbjct: 744 MKQADLAMYKAKETG---RNALRFFDPAMQTAVLERATLEAGLRKAIDGNQLLLHYQPQV 800 Query: 446 DMNTLAITGGEALVRWQHPERGLLLPGEFIPIAEETGLIVQLSDWVLDAVCRQARSWR-D 504 + +TG E LVRWQHP+RG++ PG+FIP+AEETGLI+ L +WVL+ CRQ +W Sbjct: 801 -VEGGRVTGAEVLVRWQHPQRGMVPPGDFIPLAEETGLILSLGNWVLETACRQLATWAAQ 859 Query: 505 AGLAPIRIALNVTAREFTRSLPDRVRAALN---RHSLSGDWLELEITESMLMHSTDRVIS 561 +A + IA+NV+A + PD V+ L+ R + L+LE+TES+L+ + + +I Sbjct: 860 PQMADLSIAVNVSAPQLRE--PDFVQTVLDTIERTGANPRRLKLELTESLLVDNVEDIIQ 917 Query: 562 IMEKICSLGITISLDDFGTGYSSLSYLKRFPINTLKIDRSFTMGIPDDTNDCAIASAIIG 621 M + + G+ SLDDFGTGYSSLSYLKR P+N LKID+SF + D ND +IA I+ Sbjct: 918 KMFALKAYGVEFSLDDFGTGYSSLSYLKRLPLNQLKIDQSFVRDVLIDPNDASIAKTIVN 977 Query: 622 IAKQLRHKVIAEGVETQKQFNFLREAGCNELQGYIFSRPIPAVDFHAMLLEGRKLNFVPE 681 +A+ L VIAEGVET Q +FL +AGC+ QGY F RP+ F E F P+ Sbjct: 978 LAQSLGLGVIAEGVETDAQRSFLADAGCHAYQGYFFCRPVTVEAFEKFAQE-----FDPQ 1032 Query: 682 TSA 684 SA Sbjct: 1033 FSA 1035 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,210,730 Number of extensions: 95843 Number of successful extensions: 412 Number of sequences better than 1.0e-08: 42 Number of HSP's gapped: 344 Number of HSP's successfully gapped: 42 Length of query: 692 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 600 Effective length of database: 1,184,250 Effective search space: 710550000 Effective search space used: 710550000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)