BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0036.0297.p
         (423 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311464|ref|YP_003775556.1| major facilitator superfamily p...   814   0.0  
gi|300312599|ref|YP_003776691.1| major facilitator superfamily p...    60   3e-10

>gi|300311464|ref|YP_003775556.1| major facilitator superfamily
           permease [Herbaspirillum seropedicae SmR1]
          Length = 423

 Score =  814 bits (2103), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 412/423 (97%), Positives = 419/423 (99%)

Query: 1   MNRGFYTIMAAQFFSSLADNALLIAAIALLAEMHSPAWMTPLLKLFFVLSYVLLAAFVGA 60
           MNRGFYTIMAAQFFSSLADNALLIAAIALLAEMHSPAWMTPLLKLFFVLSYVLLAAFVGA
Sbjct: 1   MNRGFYTIMAAQFFSSLADNALLIAAIALLAEMHSPAWMTPLLKLFFVLSYVLLAAFVGA 60

Query: 61  FADAFPKGKVMLITNMIKIVGCSLMFFTVHPLLAYAVVGFGAAAYSPAKYGILTELLPPE 120
           FADAFPKGKVMLITNMIKIVGCSLMFFTVHPLLAYAVVGFGAAAYSPAKYGILTELLPPE
Sbjct: 61  FADAFPKGKVMLITNMIKIVGCSLMFFTVHPLLAYAVVGFGAAAYSPAKYGILTELLPPE 120

Query: 121 KLVAANGWIEGLTVGSIIMGTVLGGALVNPKISAVILSFDFPMVDLPIDTPTEAALCIIS 180
           KLVAANGWIEGLTVGSIIMGTVLGGALVNPKIS+VILSFDFP+VDLPIDTPTEAALCIIS
Sbjct: 121 KLVAANGWIEGLTVGSIIMGTVLGGALVNPKISSVILSFDFPLVDLPIDTPTEAALCIIS 180

Query: 181 CIYIVAALFNFYIPDTGVRYEHQERNPLRLIVDFAGCFTTLWKDKLGQISLAVTTLFWGA 240
            IY++AALFNF IPDTG RYEHQERNP+RLIVDFAGCFTTLWKDKLGQISLAVTTLFWGA
Sbjct: 181 LIYVIAALFNFNIPDTGARYEHQERNPIRLIVDFAGCFTTLWKDKLGQISLAVTTLFWGA 240

Query: 241 GATLQFIVLKWAEKSLHMPLDKAAILQGIVAVGVALGAVAAARFVPLKKSLTVMPLGIIM 300
           GATLQFIVLKWAEKSLHMPLDKAAILQGIVAVGVALGA+AAARFVPLKKSLTVMPLGI+M
Sbjct: 241 GATLQFIVLKWAEKSLHMPLDKAAILQGIVAVGVALGAMAAARFVPLKKSLTVMPLGIVM 300

Query: 301 GIVVMLMTTTTSVWVAYPLLVIVGALSGYFVVPMNALLQHRGHVLMSAGHSIAVQNFNEN 360
           GIVVMLMTTTTSVWVAYPLLVIVGALSGYFVVPMNALLQHRGHVLMSAGHSIAVQNFNEN
Sbjct: 301 GIVVMLMTTTTSVWVAYPLLVIVGALSGYFVVPMNALLQHRGHVLMSAGHSIAVQNFNEN 360

Query: 361 LSVLTMLVLYAFMVRMNLDVNTVIIIFGSFVAGIMYLIMRRHAANQREHDSLALIGEDKH 420
           LSVLTMLVLYAFMVRMNLDVNTVIIIFGSFVAGIMYLIMRRHAANQREHDSLALIGEDKH
Sbjct: 361 LSVLTMLVLYAFMVRMNLDVNTVIIIFGSFVAGIMYLIMRRHAANQREHDSLALIGEDKH 420

Query: 421 PHH 423
            HH
Sbjct: 421 GHH 423


>gi|300312599|ref|YP_003776691.1| major facilitator superfamily
           permease [Herbaspirillum seropedicae SmR1]
          Length = 629

 Score = 60.5 bits (145), Expect = 3e-10,   Method: Compositional matrix adjust.
 Identities = 48/159 (30%), Positives = 72/159 (45%), Gaps = 12/159 (7%)

Query: 2   NRGFYTIMAAQFFSSLADNALLIAAIALLAEMH------SPAWMTPLLKLFFVLSYVLLA 55
            R F      QF  +L DN    A + +L           P  +  L+   F+L + L +
Sbjct: 12  QRRFAPFFWTQFLGALNDNVFKTALLTILTYDALSWTDLDPGLLNNLIPGLFILPFFLFS 71

Query: 56  AFVGAFADAFPKGKVMLITNMIKI--VGCSLMFFTVHPL--LAYAVVGFG--AAAYSPAK 109
           A  G  AD   KG+V     +++I  +G +   +  H L  L  AV+G G  +  + P K
Sbjct: 72  ATSGQLADKLEKGRVARFVKLLEIAIMGIAAYGWMTHHLWLLVAAVIGMGVHSTLFGPVK 131

Query: 110 YGILTELLPPEKLVAANGWIEGLTVGSIIMGTVLGGALV 148
           Y  L + L  E+L+  NG IE  T   I++G +LG  LV
Sbjct: 132 YAYLPQQLRREELIGGNGLIEMGTFVGILLGEILGAVLV 170


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.329    0.141    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,133,513
Number of extensions: 41593
Number of successful extensions: 211
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 211
Number of HSP's successfully gapped: 2
Length of query: 423
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 335
Effective length of database: 1,203,190
Effective search space: 403068650
Effective search space used: 403068650
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 132 (55.5 bits)