BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0293.p (453 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311468|ref|YP_003775560.1| oxidative stress resistance two... 833 0.0 gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 144 1e-35 gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbas... 123 3e-29 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 121 9e-29 gi|300311514|ref|YP_003775606.1| two component sensor histidine ... 109 4e-25 >gi|300311468|ref|YP_003775560.1| oxidative stress resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 455 Score = 833 bits (2151), Expect = 0.0, Method: Compositional matrix adjust. Identities = 416/453 (91%), Positives = 436/453 (96%) Query: 1 MKTRPQTGWFSNGLFWRTFFLLTFLITASMVAWVASFRMVERGPRAEQLAAQIVSVVTIT 60 MK R Q+GWFSNGLFWRTFFLLTFLITASMVAWVASFRMVERGPRAEQLAAQIVSVVTIT Sbjct: 3 MKVRTQSGWFSNGLFWRTFFLLTFLITASMVAWVASFRMVERGPRAEQLAAQIVSVVTIT 62 Query: 61 RAALTHSAPEMRRELLFDLASNEGIRIYPLEKSDRIVPPEDSPIVPELEYYVRQSLDENT 120 RAALTHSAPEMRRELLFDLASNEGIRIYPLEKSDRIVPPEDSPIVPELEYYVRQSLDENT Sbjct: 63 RAALTHSAPEMRRELLFDLASNEGIRIYPLEKSDRIVPPEDSPIVPELEYYVRQSLDENT 122 Query: 121 IFASSVNEVEGFWISFNIDDDQYWLMLDRGRLDRTSGLQWLGWGSIALFLSLIGAVFIST 180 +FASSVN+VEGFWISFNIDDD+YWLMLDRGRLDRTS LQWLGWGSIALFLSLIGAVFIST Sbjct: 123 LFASSVNDVEGFWISFNIDDDKYWLMLDRGRLDRTSSLQWLGWGSIALFLSLIGAVFIST 182 Query: 181 LINQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRSFNQMVADLNRIESDRALILAG 240 LINQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRSFNQMVADLNRIESDRALILAG Sbjct: 183 LINQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRSFNQMVADLNRIESDRALILAG 242 Query: 241 ISHDLRTPLARMQLEVEMANLSDESRDGMHSDLAQMDSIIGQFLDYAKPFDANSLESIDL 300 ISHDLRTPLARMQLEVEMANLSDE+RDGMHSDLAQMDSIIGQFLDYAKPFD +SLE+IDL Sbjct: 243 ISHDLRTPLARMQLEVEMANLSDEARDGMHSDLAQMDSIIGQFLDYAKPFDTSSLETIDL 302 Query: 301 AGVLGDVAAKSARLSDVKLITEITPATEVAGNGTELARVFSNLIENARRYGKTPGTDCAE 360 A VL DVA ++RLSD+++ T+ITP T+VAGNGTELARVFSNL+ENARRYGKT GTDCAE Sbjct: 303 ADVLQDVAGNASRLSDIRVHTQITPDTQVAGNGTELARVFSNLVENARRYGKTEGTDCAE 362 Query: 361 IHLRSRIEGHQVVVEVFDHGSGVPESECERLLRPFTRLDTARGQANGSGLGLAIVNRIVL 420 I +RSRIEGHQVVVEV DHG GVP +ECERLLRPFTRLDTARGQANGSGLGLAIVNRIVL Sbjct: 363 IDIRSRIEGHQVVVEVADHGPGVPVNECERLLRPFTRLDTARGQANGSGLGLAIVNRIVL 422 Query: 421 KHNGKLLLKGHEETHGGLLIQISLPLKGHRRHA 453 KHNGKLLLKGHEET GLL+QI+LPL+GHRRHA Sbjct: 423 KHNGKLLLKGHEETRSGLLVQITLPLQGHRRHA 455 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 144 bits (364), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 141/474 (29%), Positives = 218/474 (45%), Gaps = 58/474 (12%) Query: 10 FSNGLFWRTFFLLTFLITASMVA--WVAS--FRMVERGPRAEQLAAQIVSVVTITRAALT 65 F + R FFLL I ++ A W+A R R R + LA ++ +V Sbjct: 4 FLGSIGNRIFFLLLGGIVVAVFATNWLAGNERRNTLRELRFQHLADRVEQIVQAV----- 58 Query: 66 HSAPEMRRELLFDLASNEGIRIYPLEK-SDRIVP-----PEDSPIVPELEYYV------- 112 + P +R ++ ASN G +E +D I P P SP+ L+ + Sbjct: 59 DNIPPAQRPIVLQAASNFGFEARMVEDMNDAIGPDASPSPSTSPLTEMLQARLGEDRKIA 118 Query: 113 --RQS----LDENTIFASSVNEVEGFWISFNIDDDQYWLMLD----------RGRLDRTS 156 R+S L + A + +IS + DQ + L R R Sbjct: 119 VQRESECPPLRTDDARAPRRDTCRVIYISLH---DQALMRLRLHQPGEPPALRARAGPGG 175 Query: 157 GLQWLGWGSIALFLSLIG--AVFISTLINQPLARLTAAARAIAKGRQPEPLPESGPTEIE 214 G I LFL LI A ++ + +P+ +L AA + PL E+GPTEI Sbjct: 176 PASLFGLQYILLFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPLAETGPTEIR 235 Query: 215 EANRSFNQMVADLNRIESDRALILAGISHDLRTPLARMQLEVEMANLSDES-RDGMHSDL 273 +A +FN M A + R R +LA I+HDL+TPL RM+L +E +SD + + DL Sbjct: 236 QAAHAFNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLE--KVSDPGLQQKLIDDL 293 Query: 274 AQMDSIIGQFLDYAKPFDAN-SLESIDLAGVLGDVAAKSARLSDVKLITEITPATEVAGN 332 M ++ + LD A+ D++ ++++D+ +L V A +A + T A +A Sbjct: 294 GVMHGMVREGLDLARSMDSSEKIQALDIDSLLDSVCADAADAGQDVALQGSTRAFVMAQP 353 Query: 333 GTELARVFSNLIENARRYGKTPGTDCAEIHLRSRIEGHQVVVEVFDHGSGVPESECERLL 392 G L R +NLI+NA +YGK+ A +E ++ + + D G G+PESE E + Sbjct: 354 GA-LRRCLTNLIDNACKYGKSARVSIA-------LEQQKIAIRIRDQGPGIPESELEAVF 405 Query: 393 RPFTRLDTARGQ-ANGSGLGLAIVNRIVLKHNGKLLLKGHEETHGGLLIQISLP 445 PF RL+ +R + G+GLGL I I H+G+L L+ E GGL + + LP Sbjct: 406 EPFYRLEASRSRDTGGTGLGLTIALNIAENHHGQLRLRNLAE--GGLEVLLELP 457 >gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 478 Score = 123 bits (308), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 96/269 (35%), Positives = 132/269 (49%), Gaps = 14/269 (5%) Query: 183 NQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRSFNQMVADLNRIESDRALILAGIS 242 +PLA L AA A KG LPE+G E + R+F+ M + R DR + IS Sbjct: 220 TRPLAALADAAEAFGKGETVPSLPETGSREYVKTARAFSGMRERIKRYLEDRERLFVSIS 279 Query: 243 HDLRTPLARMQLEVEMANLSDESRDGMHSDLAQMDSIIGQFLDYAKPFDANSLESIDLAG 302 HDLRTP+ R++L E+ + D R H DL ++D ++ L K D + + Sbjct: 280 HDLRTPITRLKLRTELMD-DDAMRTDFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLD 338 Query: 303 VLGDVAAKSARLSDVKLITEITPA-TEVAGNGTELARVFSNLIENARRYGKTPGTDCAEI 361 L + ARL+ ++ TP+ V L R NL +NA YG+ EI Sbjct: 339 ALVLRMIRDARLAGHEI--AFTPSGINVIAKPLALKRAIGNLFDNALYYGQK-----VEI 391 Query: 362 HLRSR--IEGHQVVVEVFDHGSGVPESECERLLRPFTRLDTARGQ-ANGSGLGLAIVNRI 418 +R EG V +E+ DHG GVPE L +P+ RL+ R Q + G GLGL I I Sbjct: 392 SIRPSPGAEGDLVEIEIRDHGPGVPEDAMGTLFQPYVRLEHGRAQNSGGMGLGLGISRNI 451 Query: 419 VLKHNGKLLLKGHEETHGGLLIQISLPLK 447 V H G+LLL+ H E GGL+ I LP + Sbjct: 452 VQAHGGELLLRNHPE--GGLMATIILPAR 478 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 121 bits (304), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 92/287 (32%), Positives = 143/287 (49%), Gaps = 22/287 (7%) Query: 165 SIALFLSLIGAVFISTLINQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRSFNQMV 224 ++ +F ++ A L +PL + AA + + PL GP E+ A ++FN M Sbjct: 208 ALRIFAVVLIAWMAVRLAMKPLQVMAEAAEKLGGNIRSAPLATDGPLEVSTAAQAFNAMQ 267 Query: 225 ADLNRIESDRALILAGISHDLRTPLARMQLEVEMANLSDE-SRDGMHSDLAQMDSIIGQF 283 + ++R LA +SHDLR+P+ RM+L EM L DE ++ DL +MD++I Sbjct: 268 RRIIDNIAERTRFLAAVSHDLRSPITRMKLRTEM--LPDERGKEKFRKDLDEMDALISAT 325 Query: 284 LDYAKPFDANSL-ESIDLAGVLGDVAAKSARLSDVKLITEIT---PATEVAGNGTELARV 339 L+ + D + IDL +L +S R ++ E+T A + G L R Sbjct: 326 LNVVQDIDVQERPQLIDLDSLL-----ESLRTDQAEVGGEVTLSGHAAPLQGYPRSLRRC 380 Query: 340 FSNLIENARRYGKTPGTDCAEIHLRSRIEGHQVVVEVFDHGSGVPESECERLLRPFTRLD 399 NL+ENA RYG+ A + L+ + QV + V D G G+PE+ ER+ PF RL+ Sbjct: 381 LQNLVENAVRYGQR-----ARVQLQD--DADQVCIRVDDDGPGIPEALLERVQEPFYRLE 433 Query: 400 TARGQANGS-GLGLAIVNRIVLKHNGKLLLKGHEETHGGLLIQISLP 445 +R G GLGL+I +V H G ++K GGL ++I +P Sbjct: 434 RSRNAGTGGFGLGLSIAATVVQAHRG--VMKLVNRPGGGLRVEILIP 478 >gi|300311514|ref|YP_003775606.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 473 Score = 109 bits (273), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 82/296 (27%), Positives = 148/296 (50%), Gaps = 20/296 (6%) Query: 166 IALFLSLIGAVFISTLINQPLARLTAAARAIAKGR---QPEPLPESGPTEIEEANRSFNQ 222 +AL SL+ A+ ++ + P+ L A A G + EP+ + E+ + R F+ Sbjct: 170 VALLASLVFALLLAWYFSHPIGHLKNAFNQAANGNLDLELEPVMSARNDELADLGRDFDV 229 Query: 223 MVADLNRIESDRALILAGISHDLRTPLARMQLEVEMANLSDESR-----DGMHSDLAQMD 277 M A L + + +L +SH+LR+P+AR+Q+ + +A + D+ + + + + +MD Sbjct: 230 MTAKLRTLMESQRRLLHDVSHELRSPIARLQVAIGLARVQDKDKLDLTLERIERESVRMD 289 Query: 278 SIIGQFLDYAKPFDAN---SLES-IDLAGVLGDV---AAKSARLSDVKLITEITPATEVA 330 ++G+ L ++ +AN S+E I + +LGD+ A+ A + ++ +V Sbjct: 290 KLVGELLTLSR-LEANVPGSMEEPIAIEAMLGDMINDASFEAEAIGKHVDDQLDCRVQVK 348 Query: 331 GNGTELARVFSNLIENARRYGKTPGTDCAEIHLRSRIEGHQVVVEVFDHGSGVPESECER 390 GN L R N++ NA ++ PGT +IH + + + + D G GVPE E Sbjct: 349 GNPELLYRAIENVLRNAIKH-TAPGT-TVQIHTQLDEINKLLHLSILDRGPGVPEEEMNS 406 Query: 391 LLRPFTRLDTARGQANGSGLGLAIVNRIVLKHNGKLLLKGHEETHGGLLIQISLPL 446 + F R + ++G GLGL+I RIV H G ++ ++GGL + I+LP Sbjct: 407 IFELFFRSSRTKSNSSGHGLGLSIARRIVEAHQG--FIQAANRSNGGLRVDITLPF 460 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,327,274 Number of extensions: 52141 Number of successful extensions: 321 Number of sequences better than 1.0e-08: 32 Number of HSP's gapped: 282 Number of HSP's successfully gapped: 32 Length of query: 453 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 365 Effective length of database: 1,203,190 Effective search space: 439164350 Effective search space used: 439164350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)