BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0282.p (390 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 758 0.0 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 127 1e-30 gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 94 2e-20 gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 77 3e-15 gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 68 1e-12 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 758 bits (1957), Expect = 0.0, Method: Compositional matrix adjust. Identities = 366/384 (95%), Positives = 378/384 (98%) Query: 1 VNPLLDKLQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFIRQALTDNLSGLAS 60 VNPLLD+LQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFI+QAL DNL+GLAS Sbjct: 18 VNPLLDQLQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFIQQALIDNLAGLAS 77 Query: 61 YPATLGSDALRGAIADWLQKRYDIPRPDPATEILPVIGSREALFSLTQTVVDPTRPGALV 120 YPATLGSDALRGAIADWLQKRYDIPRPDPATEILPV GSREALFSLTQTVVDPTRPGALV Sbjct: 78 YPATLGSDALRGAIADWLQKRYDIPRPDPATEILPVNGSREALFSLTQTVVDPTRPGALV 137 Query: 121 MCPNPFYQIYEGSAYLAGAEPYFVNSDPARNFAPDFSQVPQEVWERVQLLFVCSPGNPTG 180 MCPNPFYQIYEGSAYLAGA+PYFVNSDP RNFAPDFSQVPQ+VWERVQLLFVCSPGNPTG Sbjct: 138 MCPNPFYQIYEGSAYLAGAQPYFVNSDPKRNFAPDFSQVPQDVWERVQLLFVCSPGNPTG 197 Query: 181 AVLTLEDWKELFALSDRYGFVIASDECYSEIYFKQDPPLGGMQAARLLGRDYRNLVSFSS 240 AVLTLEDWKELFALSDRYGFVIASDECYSEIYF +DPPLGGMQAARLLGRDYR L+SFSS Sbjct: 198 AVLTLEDWKELFALSDRYGFVIASDECYSEIYFNEDPPLGGMQAARLLGRDYRRLISFSS 257 Query: 241 LSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQDETHVQDNIAK 300 LSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQDETHVQ NIAK Sbjct: 258 LSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQDETHVQGNIAK 317 Query: 301 YVTKFSQVTPLLQEVMDVALPDAAFYLWAKVDKLVDISDTEFARRLYAEYNVTVLPGSYL 360 YVTKFSQVTPLLQEV++VALPDAAFYLWAKVDKLV+ISDTEFA+RLYAEYNVTVLPGSYL Sbjct: 318 YVTKFSQVTPLLQEVLEVALPDAAFYLWAKVDKLVNISDTEFAQRLYAEYNVTVLPGSYL 377 Query: 361 AREAHGVNPGAGRIRMALVAEVDE 384 AREAHGVNPGAGRIRMALVAEV+E Sbjct: 378 AREAHGVNPGAGRIRMALVAEVEE 401 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 127 bits (320), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 100/362 (27%), Positives = 170/362 (46%), Gaps = 18/362 (4%) Query: 3 PLLDKLQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFIRQALTD--NLSGLAS 60 P +++L PY F +L I + +G P TP I + L + Sbjct: 42 PRIERLPPYVFNITAELKMAARRR-GEDIIDMSMGNPDGATPPHIVEKLVEVAQRPDTHG 100 Query: 61 YPATLGSDALRGAIADWLQKRYDIPRPDPATEILPVIGSREALFSLTQTVVDPTRPGALV 120 Y ++ G LR AIA W + RY++ DP +E + IGS+E L L +D G V Sbjct: 101 YSSSKGIPRLRRAIAHWYRSRYEVDF-DPDSEAIVTIGSKEGLAHLMLATLDK---GDTV 156 Query: 121 MCPNPFYQIYEGSAYLAGAEPYFVNSDPARNFAPDFSQVPQEVWERVQLLFVCSPGNPTG 180 + PNP Y I+ A +AGA V P +F + + +E + + +++ + P NPT Sbjct: 157 LVPNPSYPIHIYGAVIAGANIRSVRMSPGVDFFDELERAVRESYPKPKMMILGFPSNPTA 216 Query: 181 AVLTLEDWKELFALSDRYGFVIASDECYSEIYFK--QDPPLGGMQAARLLGRDYRNLVSF 238 + LE ++ + L+ + ++ D Y++I F + P + + AR + V F Sbjct: 217 QCVELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVA------VEF 270 Query: 239 SSLSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQ-DETHVQDN 297 +LSK N+ G R GF+ G+A ++ ++YH +Q A++ A + D++ V++ Sbjct: 271 FTLSKSYNMAGWRIGFMVGNARLVAALARIKSYHDYGSFTPVQVAAIAALEGDQSCVEEI 330 Query: 298 IAKYVTKFSQVTPLLQEV-MDVALPDAAFYLWAKV-DKLVDISDTEFARRLYAEYNVTVL 355 A Y + + + L E V +P A+ Y+WA++ + EFAR L + V V Sbjct: 331 RANYERRRNVLVKGLHEAGWMVDVPKASMYIWARIPEPYRQFGSLEFARILLEQAKVCVS 390 Query: 356 PG 357 PG Sbjct: 391 PG 392 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 94.0 bits (232), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 88/330 (26%), Positives = 139/330 (42%), Gaps = 11/330 (3%) Query: 32 ISLGIGEPK-HPTPAFIRQALTDNLSGLASYPATLGSDALRGAIADWLQKRYDIPRPDPA 90 ++LG G P H PA + GL YP G LR AIA+ ++K Y R DP Sbjct: 31 VNLGQGFPDFHCDPALVAAVTQAMQEGLNQYPPMAGVPVLREAIAEKVEKLYG-HRYDPV 89 Query: 91 TEILPVIGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAYLAGAEPYFVNSD-PA 149 +EI G+ + + + +V+ PG V+ P Y Y + LAG P FV + A Sbjct: 90 SEITVTAGATQGILT---SVLCAVHPGDEVIVIEPVYDCYVPAIELAGGVPVFVQMEVGA 146 Query: 150 RNFAPDFSQVPQEVWERVQLLFVCSPGNPTGAVLTLEDWKELFALSDRYGFVIASDECYS 209 + ++ + +V V + +++ V +P NPTG+V+ D L + +I SDE Y Sbjct: 147 QGYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVMRAADVAALADIVRGTDILILSDEVYE 206 Query: 210 EIYFKQDPPLGGMQAARLLGRDYRNLVSFSSLSKRSNVPGMRSGFVAGDAAILKKFLLYR 269 + + P + L R + N SS K +V G + G+VA AA+ +F Sbjct: 207 HMVYDGQPHESLARHPELAERSFIN----SSFGKTYHVTGWKVGYVAAPAALTAEFRKVH 262 Query: 270 TYHGAAMSPVIQAASVLAWQDETHVQDNIAKYVTKFSQVTPLLQEVMDVALPDAAFYL-W 328 ++ ++ +Q +D D A Y K L LP Y Sbjct: 263 QFNVFTVNTPVQYGLAAYMKDPAPYLDLPAFYQHKRDLFRTGLANTRFELLPSQGTYFQC 322 Query: 329 AKVDKLVDISDTEFARRLYAEYNVTVLPGS 358 K + + + EF + L +E V +P S Sbjct: 323 VKYGAISALPEAEFCKWLTSEIGVAAIPVS 352 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 76.6 bits (187), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 81/356 (22%), Positives = 154/356 (43%), Gaps = 41/356 (11%) Query: 26 DPAYRPISLGIGEPK---HPTPAFIRQALTDNLSGLASYPATLGSDALRGAIADWLQKRY 82 + ++ I L IG P I Q + N+ + Y + G A R ++ + Q++ Sbjct: 30 EEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRKSVMHYTQQKK 89 Query: 83 -------DIPRPDPATEILPVIGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAY 135 DI + A+E+ ++ S AL + G V+ P+P Y ++ + Sbjct: 90 IEGVTIDDIYLGNGASEL--IVMSVNALLN----------TGDEVLVPSPDYPLWTAAVS 137 Query: 136 LAGAEPYFVNSDPARNFAPDFSQVPQEVWERVQLLFVCSPGNPTGAVLTLEDWKELFALS 195 L+G P D + + PD + + +++ + + V +P NPTGA+ ++E KE+ L+ Sbjct: 138 LSGGTPVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELA 197 Query: 196 DRYGFVIASDECYSEIYFKQDPPLGGMQAARLLGRDYRNLVSFSSLSKRSNVPGMRSGF- 254 ++ +I +DE Y ++ + G + D ++F+ LSK G R+G+ Sbjct: 198 RQHQLIILADEIYDKVLYD-----GNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWM 252 Query: 255 -VAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQDETHVQDNIAKYVTKFSQVTP--- 310 V+G+ + ++ A+M A A Q +I V ++T Sbjct: 253 VVSGEKKHARDYIEGLNML-ASMRLCANAPGQFAIQTALGGYQSINDLVGPQGRLTKQRD 311 Query: 311 ----LLQEVMDVAL--PDAAFYLWAKVDKLV--DISDTEFARRLYAEYNVTVLPGS 358 LL ++ V+ P +A Y++ ++D + D EFA L AE V ++ G+ Sbjct: 312 LAHQLLTDIPGVSCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEKVLIVQGT 367 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 68.2 bits (165), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 91/375 (24%), Positives = 155/375 (41%), Gaps = 43/375 (11%) Query: 1 VNPLLDKLQ---PYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFIRQALTDNLS- 56 +N L +LQ P+ +L K A + I +GIGEP P + +A T ++ Sbjct: 3 LNQLAARLQHIAPFHVMELSKK-AAVLEQQGRHLIHMGIGEPDFTAPPAVVEAATRAMTE 61 Query: 57 GLASYPATLGSDALRGAIADWLQKRYDIPRPDPATEILP---VIGSREALFSLTQTVVDP 113 G Y + G LR AI+D + Y + EI P V+ + + L Sbjct: 62 GKMQYTSATGLPQLRAAISDHYRSVYGL-------EIAPERIVVTAGASAALLLACAALV 114 Query: 114 TRPGALVM------CPNPFYQIYEGSAYLAGAEPYFVNSDPARNFAPDFSQVPQEVWERV 167 R ++M C F +EG A L + S P F V + Sbjct: 115 ERDSEVLMPDPSYPCNRHFVAAFEGRAKL-------IASGPEHRFQLSAQMVREHWGSAT 167 Query: 168 QLLFVCSPGNPTGAVLTLEDWKELFA-LSDRYGFVIASDECYSEIYFKQDPPLGGMQAAR 226 + + + SP NPTG + ++ + + + R GF I DE Y + ++ P +A Sbjct: 168 RGVLLASPSNPTGTSILPDELRAIVGEVRQRGGFTIV-DEIYQGLSYEGAP-----FSAL 221 Query: 227 LLGRDYRNLVSFSSLSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVL 286 LG D +V +S SK N+ G R G++ A++ + S + Q A+V Sbjct: 222 SLGDD---VVVINSFSKYFNMTGWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVA 278 Query: 287 AWQDETHV--QDNIAKYVTKFSQVTPLLQEVMDV--ALPDAAFYLWAKVDKLVDISDTEF 342 + +T ++ A++ + + P L+ + +PD AFY++A L D +D + Sbjct: 279 CFTPQTLALYEERKAEFKRRRDYIVPALESLGFTVPVMPDGAFYVYADCSALADDAD-QL 337 Query: 343 ARRLYAEYNVTVLPG 357 + + E V ++PG Sbjct: 338 SLDMLNEAGVVLVPG 352 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,356,984 Number of extensions: 59808 Number of successful extensions: 151 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 6 Length of query: 390 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 303 Effective length of database: 1,207,925 Effective search space: 366001275 Effective search space used: 366001275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)