BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0214.p (167 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311543|ref|YP_003775635.1| major facilitator superfamily p... 253 6e-69 gi|300310160|ref|YP_003774252.1| major facilitator superfamily p... 114 5e-27 gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 107 4e-25 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 104 4e-24 gi|300313041|ref|YP_003777133.1| major facilitator superfamily p... 104 5e-24 >gi|300311543|ref|YP_003775635.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 553 Score = 253 bits (646), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 140/144 (97%), Positives = 142/144 (98%) Query: 1 MTTASVNTTARGITPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI 60 MTTASVNTTARGITPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI Sbjct: 1 MTTASVNTTARGITPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI 60 Query: 61 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITA 120 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITA Sbjct: 61 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITA 120 Query: 121 PIILIILRILQGLALGGDPGGSXT 144 PIILIILRILQGLALGG+ GG+ T Sbjct: 121 PIILIILRILQGLALGGEYGGAAT 144 >gi|300310160|ref|YP_003774252.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 428 Score = 114 bits (284), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 58/138 (42%), Positives = 86/138 (62%), Gaps = 1/138 (0%) Query: 6 VNTTARGITPE-EKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFIFALL 64 +N +G P+ +++ + A++LG V E YDF ++G A I+K FF D A +F + Sbjct: 1 MNHPVQGTDPKTQRRAVIAATLGNVLEIYDFIIFGIFAVAISKTFFPSGDEYAALMFTFM 60 Query: 65 AFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAPIIL 124 FAAGF+VRP GALV G+ D VGRK T+++M T I + P+Y+SIGI A +IL Sbjct: 61 TFAAGFVVRPIGALVLGQYADKVGRKKALSFTLILMAIGTVIPALCPSYDSIGIAATVIL 120 Query: 125 IILRILQGLALGGDPGGS 142 + R+LQG + GG+ G + Sbjct: 121 VFGRLLQGFSAGGEIGSN 138 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 107 bits (268), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 54/139 (38%), Positives = 84/139 (60%), Gaps = 1/139 (0%) Query: 7 NTTARGITPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQ-FFAGTDPNTAFIFALLA 65 +T + + KK + +G+ E+YDF++Y + A++I Q FF DP TA I +L Sbjct: 3 STQSNAHHQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLAT 62 Query: 66 FAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAPIILI 125 + G++ RP GA V G LGD GRK L + +MG ST VG+LP Y+ +G+ AP +L+ Sbjct: 63 YGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLV 122 Query: 126 ILRILQGLALGGDPGGSXT 144 I+R+ QG A+ G+ G+ + Sbjct: 123 IMRLAQGFAVAGEISGASS 141 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 104 bits (259), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 51/127 (40%), Positives = 78/127 (61%) Query: 16 EEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFIFALLAFAAGFIVRPF 75 + ++ I A+ +G EW+DF +Y A IIAK FF + T+ + A+ F GF +RP Sbjct: 18 QRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFMRPV 77 Query: 76 GALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAPIILIILRILQGLAL 135 G ++ G D VGRK VTIL+M T ++G+ P Y SIG+ AP+I+++ R+LQG + Sbjct: 78 GGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQGFSA 137 Query: 136 GGDPGGS 142 GG+ G + Sbjct: 138 GGEMGSA 144 >gi|300313041|ref|YP_003777133.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 436 Score = 104 bits (259), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 53/142 (37%), Positives = 84/142 (59%), Gaps = 1/142 (0%) Query: 2 TTASVNTTARGITPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFIF 61 T A + R ++ + K + S+LG E+YDF +Y AA++ K FF P + Sbjct: 8 TGALNESQHRPLSSSDYKTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADMPEWLRML 67 Query: 62 ALLA-FAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITA 120 FAAG++ RP G ++ GD++GRK F ++I +M T ++G+LP Y SIG+ A Sbjct: 68 QTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAA 127 Query: 121 PIILIILRILQGLALGGDPGGS 142 PI+L+++R LQG A+GG+ G+ Sbjct: 128 PILLLLMRTLQGAAIGGEMPGA 149 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.143 0.456 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 353,517 Number of extensions: 9690 Number of successful extensions: 78 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 77 Number of HSP's successfully gapped: 11 Length of query: 167 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 88 Effective length of database: 1,245,805 Effective search space: 109630840 Effective search space used: 109630840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 127 (53.5 bits)