BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0196.p (369 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspir... 528 e-151 gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 183 2e-47 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 156 3e-39 gi|300310663|ref|YP_003774755.1| outer membrane porin, OmpC fami... 153 2e-38 gi|300313291|ref|YP_003777383.1| porin transmembrane protein [He... 149 3e-37 >gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 374 Score = 528 bits (1361), Expect = e-151, Method: Compositional matrix adjust. Identities = 272/374 (72%), Positives = 318/374 (85%), Gaps = 5/374 (1%) Query: 1 MKKSSMLLLAAGLTTGGAAFAQSSVTIYGIVDTSIRYLSNDNAQGSSNVRMDNGAISNSR 60 MKKSS+LLLAAGLT+GGAAFAQS+VTIYGIVDTSI YLSN NAQG+SNVRMDNGAI+NSR Sbjct: 1 MKKSSLLLLAAGLTSGGAAFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDNGAIANSR 60 Query: 61 IGFKGTEDLGGGLKAVFRLENGFSSDTGAAQQGSRLFGRQSWVGLAGDFGKVSVGRQNTA 120 IGFKGTEDLGGGLKA+F+LENGFSSDTGA QQG LFGRQSWVGL G FGKV VGRQNT Sbjct: 61 IGFKGTEDLGGGLKAMFQLENGFSSDTGAMQQGGSLFGRQSWVGLQGGFGKVRVGRQNTP 120 Query: 121 LFDLMADHFDPLTVGNYETNSWMPAAATRVRSDNTVRYDGQFGPVAASVSYAFGEQAGST 180 LFDL+ADHFDPLTVGNY +NSW+PAAA+ VRS N +RYDG FGPVAAS S+AFGE+AGS Sbjct: 121 LFDLLADHFDPLTVGNYASNSWLPAAASLVRSPNMLRYDGDFGPVAASASWAFGEKAGSV 180 Query: 181 RSGSQISTALRYNNGGFAIGGGFQQTVDTTTARNKATTYNLSASYAFGAAKVFAGYYRMK 240 R+GSQ S+ALRY G A+GGG+QQTV +T + K TT+NLSASY FG AKVF G+ +K Sbjct: 181 RTGSQFSSALRYTAGPLALGGGYQQTVSSTNSSLKHTTWNLSASYDFGVAKVFGGWLNIK 240 Query: 241 DATGVNGAFME-----TTNYSLANTAGGNTVGVERKDDGYFFGANYQATAALSLTGGVYY 295 D +G+ G+ M + ++ A A + GV+RKD+G+F G++Y+AT AL+LTG YY Sbjct: 241 DDSGLTGSVMAADGDVSNGWTTAGAANNGSTGVKRKDNGFFLGSSYKATNALTLTGAFYY 300 Query: 296 DKSKNVAYQALGNVGDGKRYTIVGVAEYALSKRTQLYTTVDYNKAKDAAVYEITNSANGK 355 D+SKNV+Y + GNVGDGKRYT+VG A+YALSKRTQLYTT+DYNKAKDAAVYE+TNSANGK Sbjct: 301 DRSKNVSYVSTGNVGDGKRYTLVGWADYALSKRTQLYTTIDYNKAKDAAVYELTNSANGK 360 Query: 356 SNLTTVGVGIRHIF 369 ++LTTVGVGIRHIF Sbjct: 361 NSLTTVGVGIRHIF 374 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 183 bits (465), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 138/368 (37%), Positives = 195/368 (52%), Gaps = 45/368 (12%) Query: 17 GAAFAQSSVTIYGIVDTSIRYLSNDNAQGSSNV-RMDNGAISNSRIGFKGTEDLGGGLKA 75 G+ AQSSVTIYG+VDT + YL+N G+ +V RM +GA + SRIGF+GTEDLGGGLKA Sbjct: 17 GSVQAQSSVTIYGLVDTGVEYLNNAGPNGAGSVTRMTSGATNTSRIGFRGTEDLGGGLKA 76 Query: 76 VFRLENGFSSDTGAAQ-QGSRLFGRQSWVGLAGDFGKVSVGRQNTALFDLMADHFDPLTV 134 +F+LENGF D G+ ++LF RQS VGL G FG+V GR + +D + FDP+ Sbjct: 77 LFQLENGFKLDNGSFDGDANQLFNRQSTVGLEGGFGRVVAGRSYSTTYDFLGP-FDPM-- 133 Query: 135 GNYETN-SW-MPAAATRVRSD-------NTVRYDGQFGPVAASVSYAFGEQAGSTRSGSQ 185 Y N SW A AT R D N V+Y G+F V Y FGE AG TR+G++ Sbjct: 134 -GYSANYSWATTAGATGNRKDGMPTGVSNLVKYQGEFSGVKIGAMYGFGEVAGETRNGAK 192 Query: 186 ISTALRYNNGGFAIGGGFQQ---TVDTTTARNKATTYNLSASYAFGAAKVFAGYYRMKDA 242 L + G F++ + + T T ++AT+ +L+A Y A F YR Sbjct: 193 YGVGLGWGTGPFSVVATYDRNNSATATNTPYDRATSAHLAAGYQPSEAWKFNLGYRYYKK 252 Query: 243 TGVNGAFMETTNYSLANTAGGNTVGVERKDDGYFFGANYQATAALSLTGGVYYDKSKNVA 302 + GA + + D ++ GA Y+ T A++L G +YY KNVA Sbjct: 253 SLATGA-------------------ADLRSDFFWGGAAYKFTPAMTLIGAIYYQNIKNVA 293 Query: 303 YQALGNVGDGKRYTIVGVAEYALSKRTQLYTTVDYNKAKDAAVYEITNSANGK-SNLTTV 361 N D Y++ +YA+SKRT LY + Y KAK+ + ++ G S+ + Sbjct: 294 -----NDADPIMYSL--RLKYAMSKRTDLYLSTAYAKAKNGQLVGLSRDDAGYGSSQFGM 346 Query: 362 GVGIRHIF 369 +G++H F Sbjct: 347 TLGMQHRF 354 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 156 bits (394), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 144/403 (35%), Positives = 191/403 (47%), Gaps = 47/403 (11%) Query: 1 MKKSSMLLLAAGLTTGGAAFAQSSVTIYGIVDTSIRYLSN---DNAQGSSNVRMDNGAIS 57 MKKS +L LA GAA AQSSVTIYGIVDT + Y S S +++G I Sbjct: 1 MKKS-LLALAVLGAFAGAAQAQSSVTIYGIVDTGVVYQSKVATPTGGTGSKFSLNSGVIQ 59 Query: 58 NSRIGFKGTEDLGGGLKAVFRLENGFSSDTGA---AQQGSRLFGRQSWVGLAGDFGKVSV 114 SRIGFKGTEDLGGGL AVF LE GFS+DTGA ++ + LF R+S VGLAG+FG V + Sbjct: 60 GSRIGFKGTEDLGGGLSAVFNLEAGFSNDTGALDDSKTSNTLFRRKSVVGLAGNFGSVLL 119 Query: 115 GRQNTALFDLMADHFDPLTVGNYETNSWMPAAATRV------RSDNTVRY-DGQFGPVAA 167 GRQ T D ++ + G W AA + R++N++ Y Sbjct: 120 GRQ-TDFADTISSYTSVQDFG-----GWTGAAGHNLDRLEGTRTNNSISYTTANLNGFTG 173 Query: 168 SVSYAFGEQAGSTRSGSQISTALRYNNGGFAIGGGFQQ-----------------TVDTT 210 ++ Y FGE AG T +G +Y+NG +G + Q T T Sbjct: 174 NLMYGFGETAGQTSAGQAFGVGGKYDNGPLGLGINYYQSKAGSTPADASLLAATGTNQFT 233 Query: 211 TARNKATT----YNLSASYAFGAAKVFAGYYRMKDATGVNGAFMETTNYSLANTAGGNTV 266 N +T N++ASY FG A+V+ + R+K SLA T T+ Sbjct: 234 NGTNNGSTAQKVLNVAASYQFGPARVYGNWSRVKQDLNTQANLTAAGVGSLA-TISNRTL 292 Query: 267 GVERKDDGYFFGANYQATAALSLTGGVYYDKSKNVAYQALGNVGDGKRYTIVGVAEYALS 326 K D Y G Y + +L L V + ++ Q G G K + +Y LS Sbjct: 293 AASNKADIYEIGTAYSLSPSLKLLAAVDHTRA-----QFDGVSGKAKLTQVSLGTDYWLS 347 Query: 327 KRTQLYTTVDYNKAKDAAVYEITNSANGKSNLTTVGVGIRHIF 369 KRT LY + Y+KA D + +S+ N V VGIRH F Sbjct: 348 KRTDLYAFLAYSKATDLNNPGVADSSGTDGNQGAVAVGIRHKF 390 >gi|300310663|ref|YP_003774755.1| outer membrane porin, OmpC family protein [Herbaspirillum seropedicae SmR1] Length = 346 Score = 153 bits (387), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 130/390 (33%), Positives = 191/390 (48%), Gaps = 65/390 (16%) Query: 1 MKKSSMLLLAAGLTTGGAAFAQSSVTIYGIVDTSIRYLSNDNAQGSSNV-RMDNGAISNS 59 MKK+ + +A+ L GGA AQS+VT+YG++DT Y+ NA G +V +D+G S Sbjct: 1 MKKAWAVGVASLLCAGGA-LAQSNVTLYGVIDT---YMGYTNATGKGSVASLDSGGYQAS 56 Query: 60 RIGFKGTEDLGGGLKAVFRLENGFSSDTGAAQQGSRLFGRQSWVGLAGDFGKVSVGRQNT 119 R+GFKG+EDLGGGL+A F++ENGF+SD+GA +RLF RQSWVGL + G++ +GRQN+ Sbjct: 57 RVGFKGSEDLGGGLRANFQMENGFASDSGAMHDSTRLFNRQSWVGLGNELGELRLGRQNS 116 Query: 120 ALFDLMA--DHFDPLTVGNYETNSWMPAAATRVRSDNTVRYDGQFGPVA----ASVSYAF 173 F ++A D F T ++ N +A R DN + Y PV Y Sbjct: 117 PGFLMIARLDAFAGATYASFLNN----VSAYTPRYDNVIGY---LSPVMRGFRVQAYYGL 169 Query: 174 GEQAGSTRSGSQISTALRYNNGGFAIGGGFQQTVDTTTARNKATTYNLS-------ASYA 226 GEQ S A Y +G +G +++A+N A N++ +Y Sbjct: 170 GEQTAPRNGLSTAMLAGEYESGPLYLG-------VSSSAQNSAND-NITIRSTFAGGAYD 221 Query: 227 FGAAKVFAGYYRMKDATGVNGAFMETTNYSLANTAGGNTVGVERKDDGYFFGANYQATAA 286 +G+ KVF GYYR + L A NT G Y ANY+ T Sbjct: 222 YGSGKVFLGYYRGNN---------------LGAAASANTPGSYY--SAYSLSANYRLTGQ 264 Query: 287 LSLTGGVYYDKSKNVAYQALGNVGDGKRYTIVGVAEYALSKRTQLYTTVDYNKAKDAAVY 346 +L G + K A + + ++++++G Y LS+RT LY T + + A + Sbjct: 265 ATLGAGYGWAKDSTGAGR------NARQFSLIGT--YDLSRRTMLYATYAHLANNNGASF 316 Query: 347 EITNSANGKSNLTTVG-------VGIRHIF 369 + +A N G VGIRH+F Sbjct: 317 SLAGAAPITKNAPVSGGTVNGVQVGIRHLF 346 >gi|300313291|ref|YP_003777383.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 365 Score = 149 bits (376), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 126/369 (34%), Positives = 177/369 (47%), Gaps = 48/369 (13%) Query: 22 QSSVTIYGIVDTSIRYLSNDNAQGSSNVRMDNGAIS-NSRIGFKGTEDLGGGLKAVFRLE 80 QSSVTIYGI+DT + Y +N NA G+S +M + S SRIGFKGTEDLGGGL+A+F LE Sbjct: 24 QSSVTIYGIIDTGVVYTTNANANGNSVFKMPSLTGSFPSRIGFKGTEDLGGGLQAMFVLE 83 Query: 81 NGFSSDTGAAQQGSRLFGRQSWVGLAGDFGKVSVGRQ--NTALFDLMADHFDPLTVGNYE 138 +GF+ DTGA QG RLFGRQS+VGL +G++ +GRQ T L L D P Sbjct: 84 SGFAPDTGAMGQGGRLFGRQSYVGLKNSWGQLMLGRQVNMTYLSQLKTDVMGPNLFAIGS 143 Query: 139 TNSWMPAAATRVRSDNTVRYDGQFGPVAASVSYAF----------------GEQAGSTRS 182 ++++P A RSDN + Y G F +Y+F GE AG++++ Sbjct: 144 IDAYLPNA----RSDNAIGYLGNFSGFTVGATYSFGRDAAGPAGPAATNCPGEVAGNSKA 199 Query: 183 GSQISTALRYNNGGFAIGGGFQQTVDTTTARNKATTYNLSASYAFGAAKVFAGYYRMKDA 242 Q++ L Y+ G+ + + T A N T+ + + GY+ + D Sbjct: 200 CRQVTALLGYDGKGWGVTSSYDILYGNTGASNGMTSSDNTDQRV-----TLNGYFMLGDV 254 Query: 243 TGVNGAFMETTNYSLANTAGGNTVGVERKDDGYFFGANYQATAALSLTGGVYYDKSKNVA 302 + G ++ + ANT NT D Y+ G +Y + AL L V KN Sbjct: 255 K-IGGGVVDRRTRAAANT---NT-----DSDLYYLGVSYPLSVALVLDAQVSRLDVKNSP 305 Query: 303 YQALGNVGDGKRYTIVGVAEYALSKRTQLYTTVDY--NKAKDAAVYEITNSANGKSNLTT 360 +V R T Y LSKRT +Y ++ Y N + + + N Sbjct: 306 NDVTLSV---ARLT------YNLSKRTAVYGSLGYIKNGGTSSVALDAGGTVGAGKNQLG 356 Query: 361 VGVGIRHIF 369 V GIRH F Sbjct: 357 VMTGIRHTF 365 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.130 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,103,370 Number of extensions: 48570 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 124 Number of HSP's successfully gapped: 14 Length of query: 369 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 282 Effective length of database: 1,207,925 Effective search space: 340634850 Effective search space used: 340634850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)