BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0181.p (435 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 816 0.0 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 510 e-146 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 422 e-119 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 378 e-106 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 357 1e-99 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 816 bits (2109), Expect = 0.0, Method: Compositional matrix adjust. Identities = 421/435 (96%), Positives = 431/435 (99%) Query: 1 MTYQAGAAADGARASFEDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFS 60 M+YQAGAAADGARASFEDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFS Sbjct: 1 MSYQAGAAADGARASFEDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFS 60 Query: 61 DTVYGLGAGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVSTPEMFYI 120 DTVYGLGAGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYV+TPEMFYI Sbjct: 61 DTVYGLGAGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYI 120 Query: 121 MRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPG 180 MRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPG Sbjct: 121 MRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPG 180 Query: 181 VWGHTGWQWMFILEAIPSLILGVLVILYMKDRIRDAEWLTEDEKRLLETNISHEESQKEH 240 VWGHTGWQWMFILEAIPSLILGV+VILYMKDRIRDA+WL+E+EKRLLE NISHEESQKEH Sbjct: 181 VWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKEH 240 Query: 241 LSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAA 300 LSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAA Sbjct: 241 LSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAA 300 Query: 301 AAMVLIGRSADARRERRWHVAIPAFIGCIGLLLSTLYGNNTALAMASLTLASIGILTTLP 360 AAMVLIGRSADARRERRWHVAIPAFIGCIGLLLST YG+NT LAMASLTLAS+GILTTLP Sbjct: 301 AAMVLIGRSADARRERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLP 360 Query: 361 LFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDTTQSTNAGMYVLAASLVI 420 LFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKD TQSTNAGMYVLAASLVI Sbjct: 361 LFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVI 420 Query: 421 GGLLTLSLPAKLVNK 435 GGLLTL+LPAKLVN+ Sbjct: 421 GGLLTLTLPAKLVNR 435 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 510 bits (1314), Expect = e-146, Method: Compositional matrix adjust. Identities = 248/420 (59%), Positives = 326/420 (77%), Gaps = 1/420 (0%) Query: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 +D Y KV WR++P L LCYV +YLDRVNVGFAKLQM QDL FS+TV+GLGAG+FF+GYF Sbjct: 18 KDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYF 77 Query: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVSTPEMFYIMRFLLGVAEAGFFPGV 136 +FEVPSN+++HKVGAR+WIARIMITWGI+S A +V FYI+RFLLG+AEAGF+PG+ Sbjct: 78 LFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGI 137 Query: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 ILYLTYW+P+ RR K+ A+FM+GI ++G++G PLSGWIM A G GWQWMF++EAI Sbjct: 138 ILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAI 197 Query: 197 PSLILGVLVILYMKDRIRDAEWLTEDEKRLLETNISHEESQKE-HLSLGAMFANGRVWLG 255 P++++GV +LY+ + ++ A+WL ++EK L+ +I + KE GA+ + RVWL Sbjct: 198 PAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIVKDARVWLM 257 Query: 256 ALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRE 315 LIYF FVMG YG++ W+PT++KATGV L +GLL+AIP+ A AM LIGRSAD RE Sbjct: 258 CLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRE 317 Query: 316 RRWHVAIPAFIGCIGLLLSTLYGNNTALAMASLTLASIGILTTLPLFWSLPTAFLSGTAA 375 RRWH+ IPA +G +G + + L+ +NTA+++ASL+LA+ G+LT PLFWSLPTAFLSG AA Sbjct: 318 RRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAA 377 Query: 376 AAGIALINSLGNLAGFVSPFLVGWLKDTTQSTNAGMYVLAASLVIGGLLTLSLPAKLVNK 435 A GIA INS+GNLAGFVSP+L+G+LKD T + GMY+LA LV+G + TL+ +VNK Sbjct: 378 AVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSIATLATKPGMVNK 437 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 422 bits (1085), Expect = e-119, Method: Compositional matrix adjust. Identities = 218/410 (53%), Positives = 292/410 (71%), Gaps = 1/410 (0%) Query: 18 DATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFV 77 + TY K+ WRL+P L +V +++DRVNVGFAKLQMLQDL+FS+ VYGLGAGIFF+GYF+ Sbjct: 22 EVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFI 81 Query: 78 FEVPSNMILHKVGARLWIARIMITWGIISGAMAYVSTPEMFYIMRFLLGVAEAGFFPGVI 137 FEVPSN++L K+GAR +ARI I WG+ S AMAYV+TP FYI+RFLLG+ EAGFFPGV+ Sbjct: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVV 141 Query: 138 LYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIP 197 LYLTYW+PA RR ++ LFMT A++G +GGP++G IM M GV WQW+FILE IP Sbjct: 142 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIP 201 Query: 198 SLILGVLVILYMKDRIRDAEWLTEDEKRLLETNISHEESQK-EHLSLGAMFANGRVWLGA 256 S++ G V+ ++ +R +A+WL+E EK + ++ E S +H S N RVW+ A Sbjct: 202 SILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAPGKHFSFKDACTNYRVWICA 261 Query: 257 LIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRER 316 +YFC V G ++FW P+IIK GV L +GL++AIP+ AM+ G +D ER Sbjct: 262 AVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGER 321 Query: 317 RWHVAIPAFIGCIGLLLSTLYGNNTALAMASLTLASIGILTTLPLFWSLPTAFLSGTAAA 376 R H AI I CIGL+ + + N LA+ SLTLA+IGIL P+FWS+P AFL+GTAAA Sbjct: 322 RLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAA 381 Query: 377 AGIALINSLGNLAGFVSPFLVGWLKDTTQSTNAGMYVLAASLVIGGLLTL 426 GIALINS+GNLAGFV+P+++G LK +T S ++G+Y +AA ++ L + Sbjct: 382 GGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVV 431 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 378 bits (971), Expect = e-106, Method: Compositional matrix adjust. Identities = 203/427 (47%), Positives = 288/427 (67%), Gaps = 12/427 (2%) Query: 8 AADGARASFEDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLG 67 A+ G+ F D Y KVTW++LP L L YV +YLDRVNVGFAKL ML DL S+ YGLG Sbjct: 4 ASQGSAVQF-DGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLG 62 Query: 68 AGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAY---------VSTPEMF 118 AGIFFLGYF+FEVPSN+ILH+VGA++WIARIMITWG+IS MA+ VS +F Sbjct: 63 AGIFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVF 122 Query: 119 YIMRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAM 178 Y +RFLLG +EAGFFPGVILY+TYWYPAA ++ A+ + G S ++GGP+SG +M M Sbjct: 123 YTLRFLLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFM 182 Query: 179 PGVWGHTGWQWMFILEAIPSLILGVLVILYMKDRIRDAEWLTEDEKRLLETNISHEESQK 238 GWQWMF++EA+P+ +LG++++ + + I A+WL +EK L ++ E +K Sbjct: 183 GSGDSLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEK 242 Query: 239 EHLSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAF 298 ++ L +++A +W AL+Y ++G YG++FW PTII+ GV L++G+++AIPY Sbjct: 243 VNVPLKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYIL 302 Query: 299 AAAAMVLIGRSAD-ARRERRWHVAIPAFIGCIGLLLSTLYGNNTALAMASLTLASIGILT 357 A AMV I R A+ A + R + VA+ + + + L+L TL+ +N + +A + +A G LT Sbjct: 303 GATAMVFITRHAEKAGKHRSYSVAV-SMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLT 361 Query: 358 TLPLFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDTTQSTNAGMYVLAAS 417 ++ LFW P A+ +G AAAGIA+INS+GNL GFV P+L+G L S G+ +L + Sbjct: 362 SIALFWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGGA 421 Query: 418 LVIGGLL 424 L + G+L Sbjct: 422 LFLAGVL 428 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 357 bits (915), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 189/415 (45%), Positives = 271/415 (65%), Gaps = 6/415 (1%) Query: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 EDAT KV RL+P + LCYV SYLDR+NVGFA L M +DL + + +GLGAG+FF+GYF Sbjct: 20 EDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 79 Query: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVSTPEMFYIMRFLLGVAEAGFFPGV 136 FE+PSN+ LH+ GAR+WI+RIMI+WG+IS A A+V P+ F + RFLLG+AEAGF PG+ Sbjct: 80 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 139 Query: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 LY T W+P A RGK TA F+ GI ++ +IG PLSG +M + G+WG GWQ + +LEA+ Sbjct: 140 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALME-LHGMWGFKGWQVLLLLEAL 198 Query: 197 PSLILGVLVILYMKDRIRDAEWLTEDEKRLLETNISHEES---QKEHLSLGAMFANGRVW 253 P+++LGV+ + + DR A+WL+ DEK+ LE +S E++ + L F N RV+ Sbjct: 199 PAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVF 258 Query: 254 LGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADAR 313 A FC ++G + WLP II+ G+ VGL+ AIPY A AM L R A+ Sbjct: 259 ALAGANFCGIIGSLSIGLWLPQIIREFGLPSH-QVGLVAAIPYLVGAVAMTLWARLANRS 317 Query: 314 RERRWHVAIPAFIGCIGLLLSTLYGNNTALAMASLTLASIGILTTLPLFWSLPTAFLSGT 373 R + VA + + L +S + L M ++T+A IL+ FW++P+ FL+G Sbjct: 318 ERRLFFVAGAIVLAALSLGISAFL-HTPLLKMLAITVAVASILSFQATFWAIPSGFLTGR 376 Query: 374 AAAAGIALINSLGNLAGFVSPFLVGWLKDTTQSTNAGMYVLAASLVIGGLLTLSL 428 AAA G+ALI S+GNL GFV P ++G++++ TQ + +A +L++G ++TL+L Sbjct: 377 AAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVITLAL 431 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,401,230 Number of extensions: 62144 Number of successful extensions: 410 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 372 Number of HSP's successfully gapped: 22 Length of query: 435 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 347 Effective length of database: 1,203,190 Effective search space: 417506930 Effective search space used: 417506930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)