BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0179.p (433 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 803 0.0 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 279 4e-76 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 273 3e-74 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 261 9e-71 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 256 2e-69 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 803 bits (2073), Expect = 0.0, Method: Compositional matrix adjust. Identities = 393/433 (90%), Positives = 409/433 (94%), Gaps = 4/433 (0%) Query: 1 MKQHFIDNRWVAPASGESIPVIDPSDGQAFEAIARGNAADIDQAVQAARRAYESAWGKLS 60 MKQHFI NRWVAP SGESIPV+DPSDGQ F+AIARGNA DIDQAV+AAR AYE AWG+LS Sbjct: 1 MKQHFIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLS 60 Query: 61 AAERSRLLMKLAHKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHG 120 AAERSRLLMKLA+KLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHG Sbjct: 61 AAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHG 120 Query: 121 DTIPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSL 180 DTIPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLS+ Sbjct: 121 DTIPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSI 180 Query: 181 LRVAELAAEAGFPEGALNIVTGYGHEAGDLLVKHPDVDHVSFTGSPNVGKLVVRAAAEHH 240 LRVAELAAE GFPEGALNIVTGYGHEAGDLLVKHPDV+H+SFTGSPNVGKLVVRAAAEHH Sbjct: 181 LRVAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHH 240 Query: 241 TPVTLELGGKSPQVVFEDADMDALLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERL 300 TPVTLELGGKSPQ+VFEDADM+A+LPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERL Sbjct: 241 TPVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERL 300 Query: 301 AGLFSQLKVGSSQMDLDCGPLIRKTQQERVAGFLSDARRDGIALVAQGEIAPEAPAGGFY 360 +GLFSQL+VGSSQMDLDCGPLIRKTQQERVAGFLSDA+RDGIAL AQG+IA P GGFY Sbjct: 301 SGLFSQLQVGSSQMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVPTGGFY 360 Query: 361 QTPALLRDVPPQHRLAQEEVFGPVLAAMSFEDEAEAIALANGTDYGLVASVWTRDGGRQL 420 Q PALLRDVPP+HRLAQEEVFGPVLAAM FEDEA AIALANGTDYGLVASVWTRDGGRQ Sbjct: 361 QAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGGRQ- 419 Query: 421 GWTMXKTIAIRHG 433 M AIR G Sbjct: 420 ---MRLARAIRSG 429 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 279 bits (713), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 178/425 (41%), Positives = 249/425 (58%), Gaps = 14/425 (3%) Query: 3 QHFIDNRWVAPASGESIPVIDPSDGQAFEAIARGNAADIDQAVQAARRAYES-AWGKLSA 61 QHFI + G+ + +++P + + + + A+G AAD+ +AV AA+ E AW KLS Sbjct: 6 QHFIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAWSKLSG 65 Query: 62 AERSRLLMKLAHKLQDHQEELAQLEGRDCGK-PMKQARADAAAIVRYFEFYAGAADKLHG 120 +R +LL KLA ++ + LA ++ R G+ PM+ D + + AG A+++ G Sbjct: 66 LQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWANQMEG 125 Query: 121 DTIPYQAGYT-----VLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAED 175 TIP AGY T+REP GV G I+PWN P+ I V LAAG T V+KPAE+ Sbjct: 126 RTIP-TAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEE 184 Query: 176 ACLSLLRVAELAAEAGFPEGALNIVTGYGHEAGDLLVKHPDVDHVSFTGSPNVGKLVVRA 235 S L +A LA EAGFP+G +N+VTGYG+ G L +HPDV +SFTGSP G+ + R Sbjct: 185 TPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRI 244 Query: 236 AAEHHTPVTLELGGKSPQVVFEDADM-DALLPVVVNAIVQNAGQTCSAGSRLLVQRGAYE 294 A E VTLELGGKSPQ+VF+DA DAL + V N GQ C+AGSR+LVQR + Sbjct: 245 AGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFV-NQGQVCAAGSRILVQRSLAQ 303 Query: 295 QVLERLAGLFSQLKVGS-SQMDLDCGPLIRKTQQERVAGFLSDARRDGIALVAQGEIAPE 353 + + LA + + VG SQ + GP+ +K Q ERV ++ +G L+A G P+ Sbjct: 304 RFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRPD 363 Query: 354 APAGGFYQTPALLRDVPPQHRLAQEEVFGPVLAAMSFEDEAEAIALANGTDYGLVASVWT 413 G++ P + D +A+EE+FGPV +SF+ E EAIALAN + YGL A+VWT Sbjct: 364 K---GWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWT 420 Query: 414 RDGGR 418 + R Sbjct: 421 TNLAR 425 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 273 bits (697), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 181/420 (43%), Positives = 237/420 (56%), Gaps = 19/420 (4%) Query: 4 HFIDNRWVAPASGESIPVIDPSDGQAFEAIARGNAADIDQAVQAARRAYESAWGKLSAAE 63 HFI R+ P + + IDP+ G+ +++G+AAD+D AVQAA+ A W L Sbjct: 35 HFIAGRFTQPKA--DLDDIDPASGKLLAHLSQGSAADVDAAVQAAQAAL-PGWQALGGHG 91 Query: 64 RSRLLMKLAHKLQDHQEELAQLEGRDCGKPMKQAR-ADAAAIVRYFEFYAGAADKLHGDT 122 R+R L LA +Q H LA LE D GKP++++R D + R+F YAG A +L Sbjct: 92 RARHLYALARTVQRHARLLAVLETLDNGKPLRESRDVDVPLVARHFYHYAGWA-QLQESE 150 Query: 123 IPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSLLR 182 P P GV G IVPWN+P+ + + ALA GNT V+KPAE+ L+ L Sbjct: 151 FPDHV---------PVGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAENTSLTALL 201 Query: 183 VAELAAEAGFPEGALNIVTGYGHEAGDLLVKHPDVDHVSFTGSPNVGKLVVRAAAEHHTP 242 AELA +AG P G LNIVTG G G +V HP + ++FTGS VG+L+ A Sbjct: 202 FAELAQQAGLPAGVLNIVTGDG-ATGAAVVAHPGIQKIAFTGSTEVGRLIREQTAGSGKS 260 Query: 243 VTLELGGKSPQVVFEDADMDALLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERLAG 302 +TLELGGKSP +VFEDAD+DA + VV+AI N GQ C AGSRLLVQ G ++ + RL Sbjct: 261 LTLELGGKSPFIVFEDADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFIARLKT 320 Query: 303 LFSQLKVGSSQMDL-DCGPLIRKTQQERVAGFLSDARRDGIALVAQGEIAPEAPAGGFYQ 361 +LKVG+ D G LI Q ERV + R+G ++ + P GG + Sbjct: 321 RMQKLKVGAPLDKCSDMGALISPVQLERVRSLVEQGVREGAQC---HQVVLDTPPGGCFY 377 Query: 362 TPALLRDVPPQHRLAQEEVFGPVLAAMSFEDEAEAIALANGTDYGLVASVWTRDGGRQLG 421 P LL V P +A EE+FGPVL AMSF EA+ LAN + YGL AS+W+ G LG Sbjct: 378 PPTLLTGVHPAATVASEEIFGPVLVAMSFRTPDEAVQLANNSRYGLAASIWSETIGLALG 437 Score = 69.7 bits (169), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 63/220 (28%), Positives = 91/220 (41%), Gaps = 3/220 (1%) Query: 5 FIDNRWVAPASGESIPVIDPSDGQAFEAIARGNAADIDQAVQAARRAYESAWGKLSAAER 64 +I + V P GE + G+ E + GN DI AV AA W S R Sbjct: 521 YIGGKQVRP-DGEVSMACHSTQGERLEDVGLGNRKDIRNAVAAA--VKAGGWTSASPHRR 577 Query: 65 SRLLMKLAHKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHGDTIP 124 ++ L +A L EE A+ G +ARA+ A ++ Y ADK G Sbjct: 578 AQGLYYIAENLSARTEEFARRIASTTGVTADEARAEVDASIKRLFTYGAWADKFEGAVHQ 637 Query: 125 YQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSLLRVA 184 L + E GV G + P P+ V +A GN VV P+E A L + Sbjct: 638 PPMRGVALAMPEAIGVVGVVCPEEKPLLALISLVAPLIAMGNRVVVVPSEQAPLVATDLY 697 Query: 185 ELAAEAGFPEGALNIVTGYGHEAGDLLVKHPDVDHVSFTG 224 ++ + P G +NIVTG E +L +H +V+ + G Sbjct: 698 QVLDTSDLPAGVINIVTGKSAELLPVLAEHDEVEALWVRG 737 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 261 bits (667), Expect = 9e-71, Method: Compositional matrix adjust. Identities = 172/416 (41%), Positives = 224/416 (53%), Gaps = 5/416 (1%) Query: 1 MKQHFIDNRWVAPASGESIPVIDPSDGQAFEAIARGNAADIDQAVQAARRAYESAWGKLS 60 + +ID +W P E V+ P+ + IA GNAAD+DQAVQAARRA AW S Sbjct: 15 LDHFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAI-PAWAATS 73 Query: 61 AAERSRLLMKLAHKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHG 120 ER+ LL ++ L + E AQ + G P+ AR A + E A D L Sbjct: 74 PLERAALLGRVHALLLERSELFAQALTLEMGAPISYART--AHVPLAAEHLRVARDNLAD 131 Query: 121 DTIPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSL 180 G T + +REP GV I PWN+P+ VG ALAAG T V+KP+E + LS Sbjct: 132 YPFIRPRGTTAI-VREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSA 190 Query: 181 LRVAELAAEAGFPEGALNIVTGYGHEAGDLLVKHPDVDHVSFTGSPNVGKLVVRAAAEHH 240 L AE+ EAG P G N+V G G E G L HP VD +S TGS G LV +AAA Sbjct: 191 LLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTV 250 Query: 241 TPVTLELGGKSPQVVFEDADMDALLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERL 300 V ELGGKSP +V DAD++ +P V A ++N GQ+CSA +RL+V R A +V E Sbjct: 251 KRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELA 310 Query: 301 AGLFSQLKVGS-SQMDLDCGPLIRKTQQERVAGFLSDARRDGIALVAQGEIAPEAPAGGF 359 +Q+KVG + GPL + Q RV + +DG L+A G P+ A G+ Sbjct: 311 LATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGY 370 Query: 360 YQTPALLRDVPPQHRLAQEEVFGPVLAAMSFEDEAEAIALANGTDYGLVASVWTRD 415 Y P + +V +AQEE+FGPVLA + ++ EAIA+AN T YGL A V RD Sbjct: 371 YARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRD 426 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 256 bits (654), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 156/430 (36%), Positives = 241/430 (56%), Gaps = 24/430 (5%) Query: 4 HFIDNRWVAPASGESIPVIDPSDGQAFEAIARGNAADIDQAVQAARRAYESAWGKLSAAE 63 +FI ++V P GE I P G+AF +AR +A D++ A+ AA A +S WGK S E Sbjct: 21 NFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKS-WGKTSPTE 79 Query: 64 RSRLLMKLAHKLQDHQEELAQLEGRDCGKPMKQARA-DAAAIVRYFEFYAGAADKLHGDT 122 R+ +L+K+A +++ + E LA E D GKP+++ A D + +F ++A A G Sbjct: 80 RANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGSI 139 Query: 123 IPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSLLR 182 P EP GV G I+PWN+P+ + + ALAAGN V+KPAE S++ Sbjct: 140 CPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIMV 199 Query: 183 VAELAAEAGFPEGALNIVTGYGHEAGDLLVKHPDVDHVSFTGSPNVGKLVVRAAAEHHTP 242 + EL A+ P G +NIV G+G EAG L + + ++FTG G+L+++ A+++ P Sbjct: 200 LIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLIP 258 Query: 243 VTLELGGKSPQVVFEDA-------DMDALLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQ 295 VTLELGGKSP + F D AL + A+ N G+ C+ SR+LVQ YE+ Sbjct: 259 VTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFAL--NQGEVCTCPSRVLVQESIYER 316 Query: 296 VLERLAGLFSQLKVGSSQMDLDCGPLI----RKTQQERVAGFLSDARRDGIALVAQG--- 348 +ER + +K G+ LD +I + Q E++ ++ +++G ++A G Sbjct: 317 FIERALKRVAAIKQGNP---LDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGRE 373 Query: 349 EIAPEAPAGGFYQTPALLRDVPPQHRLAQEEVFGPVLAAMSFEDEAEAIALANGTDYGLV 408 E+ + A G+Y P + + + R+ QEE+FGPV++ +F+DE EA+A+AN T YGL Sbjct: 374 ELGGDL-ASGYYVKPTVFQG-NNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLG 431 Query: 409 ASVWTRDGGR 418 A +WTRDG R Sbjct: 432 AGLWTRDGTR 441 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,511,929 Number of extensions: 68135 Number of successful extensions: 336 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 265 Number of HSP's successfully gapped: 22 Length of query: 433 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 345 Effective length of database: 1,203,190 Effective search space: 415100550 Effective search space used: 415100550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)