BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0161.p (533 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 985 0.0 gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 124 1e-29 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 82 1e-16 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 985 bits (2547), Expect = 0.0, Method: Compositional matrix adjust. Identities = 481/564 (85%), Positives = 513/564 (90%), Gaps = 31/564 (5%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKX------------------- 41 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDK Sbjct: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAAGQ 60 Query: 42 ------------TTGPGANHLVAGLYEARKDHAPVLAISGGVPAAKRGTDYLQENSPDML 89 TTGPGANHLVAGLYEARKDHAPVLAISGGVPAA RGTDYLQENSP++L Sbjct: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 Query: 90 FRDVAAYSQVITSPEQAPAVFHQAIAQAYAQRGVAHLNIPADIIGAKVSGTVPSIHTLRE 149 FRDVAAYSQ+ITSP+QAPAVFHQAIAQAYAQRGVAHLNIPAD+IGAK SG+VPS+HTLRE Sbjct: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 Query: 150 CSELAPPAAEIAAAAELINAAGSVAIFAGNGCRGSIDQVLALASKLQAPVMHTFRGKDLV 209 + L+PPAA+I AAA+LINAAGSVAIFAGNGCRG+ID+VLALASKLQAPVMHTFRGKDL Sbjct: 181 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLA 240 Query: 210 AYDHPHWIGGVGLIGGAPGTDALHGAEVMLMLGSDYPYSEFLPTKAKIIQIDERGFVLGR 269 +YDHPHWIGGVGLIGGAPGTDALH AEVMLMLGSDYPYSEFLPTK+K+IQIDERGFVLGR Sbjct: 241 SYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR 300 Query: 270 RAPVTLGITGSVGPAVQALLEKVDGKTDGSFLQKANADRERWNSKLDSAAEIKRHHKKIK 329 RAPVTLGITGSV PAVQ LLE+V+GKTDG+FLQ+ANA RERWN+KLDSAAEIKRHHKKIK Sbjct: 301 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIK 360 Query: 330 PQSVARALSDLADDDARFVIDTGVVTLWCGNWIRQRGQQRLLASFNNAAVGTSLGQANGI 389 PQSVARA+SDLADDDA FV+DTGVVTLWCGNWIRQRG QR+LASFNNAAVGTSLGQANGI Sbjct: 361 PQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGI 420 Query: 390 QALDRERQVIVGVGDGGFTMLLGEFMTSVEHKLPVKVIVFNNREWGLVHLEMEEAGMPAF 449 QALDRERQV+V VGDGGFTMLLGEFMTSVEH+LPVKV+VFNNREWGLVHLEMEEAGMPAF Sbjct: 421 QALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPAF 480 Query: 450 EGSEFPNADFAAFARACGGQGFTARAPEELQESLQQLLAAPGPAVLDVFIDPSELPVMPH 509 EGSEFPN DFAAFARACGGQGF+ R PEEL+ LQQLLAAPGPAVLDVFIDP ELPVMPH Sbjct: 481 EGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPH 540 Query: 510 IKMEQIWKFGLAKVKESLIAMQGG 533 IKMEQIW+FGLAKV+ESLIAM+GG Sbjct: 541 IKMEQIWRFGLAKVRESLIAMKGG 564 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 124 bits (312), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 134/557 (24%), Positives = 220/557 (39%), Gaps = 55/557 (9%) Query: 4 TVADILIDTLYEIGVRQIFGVVGDALNPLTDAI-RRDK---------------------- 40 T ADIL+ L E GV +FG G A+ + DAI ++DK Sbjct: 7 TGADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRS 66 Query: 41 --------XTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAKRGTDYLQENSPDMLFRD 92 T+GPG + V GL A D P++ ISG VP+ G D QE + R Sbjct: 67 SQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDTVGITRP 126 Query: 93 VAAYSQVITSPEQAPAVFHQAIAQAYAQR-GVAHLNIPADIIGAKVSGTVPSIHTLRECS 151 ++ ++ + +A A R G ++IP DI + P +R Sbjct: 127 CVKHNFLVKDVKDLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCAYAYPKAVEMRSYK 186 Query: 152 ELAP-PAAEIAAAAELINAAGSVAIFAGNGC--RGSIDQVLALASKLQAPVMHTFRGKDL 208 + + +I A +L+ +A I+ G G + ++ L +L P +T G Sbjct: 187 PVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNTLMGLGA 246 Query: 209 VAYDHPHWIGGVGLIGGAPGTDALHGAEVMLMLGSDYPY------SEFLPTKAKIIQIDE 262 ++G G+ G A+ +V++ +G+ + F KII ID Sbjct: 247 YRSSSDKFVGMPGMHGTYEANMAMQHCDVLIAIGARFDDRVIGNPKHFASHARKIIHIDI 306 Query: 263 RGFVLGRRAPVTLGITGSVGPAVQALLEKVD-------GKTDGSFLQKANADRERWNSKL 315 + +R V + I G+V +Q LL ++D G ++ ++ N R+R K Sbjct: 307 DPSSISKRVKVDIPIVGNVKDVLQELLSQLDAAETRQNGAALDAWWKQINEWRKRDCLKF 366 Query: 316 DSAAEIKRHHKKIKPQSVARALSDLADDDARFVIDTGVVTLWCGNWIRQRGQQRLLASFN 375 ++ E IKPQSV + + ++ DA D G +W + +R + S Sbjct: 367 ATSEEF------IKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG 420 Query: 376 NAAVGTSLGQANGIQALDRERQVIVGVGDGGFTMLLGEFMTSVEHKLPVKVIVFNNREWG 435 +G L A G+Q + + V G+ M + E T ++ L K+I+ NNR G Sbjct: 421 LGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG 480 Query: 436 LV-HLEMEEAGMPAFEGSEFPNADFAAFARACGGQGFTARAPEELQESLQQLLAAPGPAV 494 +V + + G E DF A + G G P ++ +L++ A V Sbjct: 481 MVRQWQQIDYGSRYSESYMDSLPDFNKLAESYGHVGMKIEKPGDVDGALREAFAMKDRLV 540 Query: 495 LDVFIDPSELPVMPHIK 511 FI V P +K Sbjct: 541 FMNFITDQTENVWPMVK 557 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 82.0 bits (201), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 117/500 (23%), Positives = 207/500 (41%), Gaps = 47/500 (9%) Query: 42 TTGPGANHLVAGLYEARKDHAPVLAISGGVPAAKRGTDYLQE----NSPDM----LFRDV 93 + GPGA +L+ A + PVL + G V ++R LQ+ N P + FR + Sbjct: 97 SIGPGATNLLTAAALAHVNRLPVLLLPGDVFVSRRPDPVLQQLEDFNDPGLSVNDAFRPL 156 Query: 94 AAYSQVITSPEQAPAVFHQAIAQAY--AQRGVAHLNIPADIIGAKVSGTV----PSIHTL 147 + I PEQ +AI AQ G L +P D+ + P + Sbjct: 157 SRMFDRICYPEQLLTALPRAIQVLTDPAQCGPVTLALPQDVQTMAYDYPLDFFSPRVIVP 216 Query: 148 RECSELAPPAAEIAAAAELINAAGSVAIFAGNGCR--GSIDQVLALASKLQAPVMHTFRG 205 R AP A E+ A L+ A + AG G + + + A + PV T G Sbjct: 217 RA---QAPAAQELEDAVALLRQARQPLLIAGGGVLYGNACEHLRRFAEQHGVPVAETQAG 273 Query: 206 KDLVAYDHPHWIGGVGLIGGAPGTDAL-HGAEVMLMLGS---DYPY-SEFLPTKAKIIQI 260 K + + H +G +G + G+P +AL A+V++ +G+ D+ S L +A+++ + Sbjct: 274 KSALPWSHALQMGAIG-VTGSPAANALAQEADVVIAVGTRLQDFTTGSHTLFAQARLLNL 332 Query: 261 DERGFVLGRRAPVTLGITGSVGPAVQALLEKVDG-KTDGSFLQKANADRERWNSKL---- 315 + + L + G ++AL ++G ++ + +A+ + W ++ Sbjct: 333 NVNAMDAHKWR--GLALQADAGLGLEALSHALEGWRSAPEWHARAHTLAQDWRERVAQIT 390 Query: 316 ---DSAAEIKRHHKKIKPQSVARALSDLADDDARFVIDTGVVTLWCGNWIRQRGQQRLLA 372 D+ + + I ++ R+++D D V G + R Sbjct: 391 GQTDTGGRLPYDGEVIG--AIQRSVADSPSQDI-VVCAAGTLPAELHKLWRAGRPGAYHV 447 Query: 373 SFNNAAVGTSLGQANGIQALDRERQVIVGVGDGGFTMLLGEFMTSVEHKLPVKVIVFNNR 432 + + +G + G++ +R+VIV VGDG + M+ E TSV + V+V +NR Sbjct: 448 EYGYSCMGYEVAGGLGVKLAQPQREVIVIVGDGSYLMMNSELATSVMLGAKLIVVVLDNR 507 Query: 433 EWGLVHLEMEEAGMPAFE---------GSEFPNADFAAFARACGGQGFTARAPEELQESL 483 +G ++ + G F G P DFAA AR+ G G + EL+ +L Sbjct: 508 GYGCINRLQQACGGAPFNNMLADCLQAGPGAPAIDFAAHARSLGALGENVKTITELEAAL 567 Query: 484 QQLLAAPGPAVLDVFIDPSE 503 Q+ AA ++ + D S Sbjct: 568 QRARAADRSYLVCIDTDASR 587 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,625,184 Number of extensions: 67506 Number of successful extensions: 224 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 4 Length of query: 533 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 443 Effective length of database: 1,193,720 Effective search space: 528817960 Effective search space used: 528817960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)