BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0151.p (294 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310071|ref|YP_003774163.1| VGR-related protein [Herbaspiri... 195 3e-51 gi|300310083|ref|YP_003774175.1| VGR-related type VI secretion s... 66 3e-12 gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion s... 61 1e-10 gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspiri... 57 2e-09 gi|300310098|ref|YP_003774190.1| VGR-related protein [Herbaspiri... 57 2e-09 >gi|300310071|ref|YP_003774163.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 954 Score = 195 bits (496), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 90/113 (79%), Positives = 101/113 (89%) Query: 25 LSGFPSSLFGPGLSQHARLITLASAQEAGLPESLVVERFHGQESVNDNFQFDIDALSVST 84 L+ + LFG GLSQ+ARLIT+ +AQ AGLPE+L VERF G+ESVNDNF+FDIDALS+ST Sbjct: 9 LTAAVTELFGTGLSQNARLITMTTAQSAGLPETLAVERFEGEESVNDNFRFDIDALSIST 68 Query: 85 DLDLKQFIGTELTLRLLQADGSRRAWHGYCTQASWLGADGGLARYRLRLESFL 137 DLDLKQFIG E++LRLLQADG RAWHGYCTQASWLGADGGLARYRLRLESFL Sbjct: 69 DLDLKQFIGAEISLRLLQADGGTRAWHGYCTQASWLGADGGLARYRLRLESFL 121 >gi|300310083|ref|YP_003774175.1| VGR-related type VI secretion system protein [Herbaspirillum seropedicae SmR1] Length = 944 Score = 65.9 bits (159), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 56/180 (31%), Positives = 88/180 (48%), Gaps = 21/180 (11%) Query: 25 LSGFPSSLFGPGLSQHARLITLASA--QEAGLPESLVVERFHGQESVN------------ 70 LS P+ LFG SQ AR++ L + Q+ G P +L+ ER E V Sbjct: 3 LSDLPA-LFGA-WSQSARVMRLHTPLDQDYG-PHALMAERLQAVEQVGPGEDSSAIDQAI 59 Query: 71 DNFQFDIDALSVSTDLDLKQFIGTELTLRLLQADGSRRAWHGYCTQASWLGADGGLARYR 130 ++ + LS++ D+ L++ +G + L L DG RA+HG+ T GA+GG+ARY Sbjct: 60 AGYRLQLSCLSLNADISLRKLLGQPVLLEL-DTDGGARAFHGHVTACRMEGANGGMARYL 118 Query: 131 LRLESFLDIRLNREELRKKWGIDTPNVSEAEKSLFAEFRQAKTEQAAARQQLAKAAKASK 190 L +E +L +R + + P + EA +FA++R Q A R L A+ +K Sbjct: 119 LTIEPWLSFLRHRRDSAVFQDMSAPAIIEA---VFADYRGQGRLQPAWRLDLQDASAYAK 175 >gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion system protein [Herbaspirillum seropedicae SmR1] Length = 886 Score = 60.8 bits (146), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 38/111 (34%), Positives = 58/111 (52%), Gaps = 3/111 (2%) Query: 37 LSQHARLITLASAQEAGLPESLVVERFHGQESVNDNFQFDIDALSVSTDLDLKQFIGTEL 96 ++QH RL+ L S+ P+ L+ +R E +ND ++ ID L+ T + L+Q I + Sbjct: 16 ITQHRRLLQLDSSLG---PDILLPQRLVATERLNDGYEVTIDLLATHTGIALEQLIAQPV 72 Query: 97 TLRLLQADGSRRAWHGYCTQASWLGADGGLARYRLRLESFLDIRLNREELR 147 TL + Q DG HGY A LG+DG L +L L +L R++ R Sbjct: 73 TLWIRQHDGDTLPLHGYVHTAKRLGSDGELDFCQLSLSPWLHFLRFRKDAR 123 >gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 928 Score = 57.0 bits (136), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 41/126 (32%), Positives = 63/126 (50%), Gaps = 9/126 (7%) Query: 23 TLLSGFPSSLFGPGLSQHARLITLASAQEAGLPESLVVERFHGQESVNDNFQFDIDALSV 82 T LS + G Q R++ L S P+ L+ +R G E + + + ID +S Sbjct: 18 TTLSALADTFVG----QEKRILKLDSVLG---PDVLLPQRMVGSERLGRGYDYTIDLVST 70 Query: 83 STDLDLKQFIGTELTLRLLQADGSRRA-WHGYCTQASWLGADGGLARYRLRLESFLDIRL 141 D++LK+ I +TL LQ DGS A HGY A LG+DG LA +++ + +L Sbjct: 71 RDDIELKKLIAQPVTL-WLQQDGSDYAPLHGYVHTARRLGSDGQLASFQIGMAPWLHFLK 129 Query: 142 NREELR 147 R++ R Sbjct: 130 YRKDAR 135 >gi|300310098|ref|YP_003774190.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 982 Score = 56.6 bits (135), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 35/90 (38%), Positives = 50/90 (55%), Gaps = 3/90 (3%) Query: 44 ITLASAQEAGLPESLVVERFHGQESVNDNFQFDIDALSVSTDLDLKQFIGTELTLRLLQA 103 ++L A AG L+VE F E ++ ++ LS+ L LK IG TL + Sbjct: 20 LSLGDAAPAG---ELLVEAFAASEGLHAVGLREVIVLSLDAGLRLKSLIGQRATLVTTLS 76 Query: 104 DGSRRAWHGYCTQASWLGADGGLARYRLRL 133 DGSR + G +A+ LG++GGLARYRLR+ Sbjct: 77 DGSRTQFSGLVNEAAKLGSEGGLARYRLRI 106 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.129 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 817,375 Number of extensions: 30225 Number of successful extensions: 90 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 90 Number of HSP's successfully gapped: 7 Length of query: 294 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 210 Effective length of database: 1,222,130 Effective search space: 256647300 Effective search space used: 256647300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)