BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0143.p (312 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311595|ref|YP_003775687.1| two component response regulato... 528 e-151 gi|300310564|ref|YP_003774656.1| regulator of the two-component ... 217 1e-57 gi|300312232|ref|YP_003776324.1| signal transduction protein [He... 60 2e-10 gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626... 59 5e-10 >gi|300311595|ref|YP_003775687.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 310 Score = 528 bits (1360), Expect = e-151, Method: Compositional matrix adjust. Identities = 258/310 (83%), Positives = 282/310 (90%), Gaps = 2/310 (0%) Query: 1 MSHYVDPKVFRELQDSEVLPAPSGVRLKIMQMCQQEDIALPDLVAQVQGDPVLAGRLIQI 60 MS YVDP+ FRE+QDSEVLPAPSGVRLKIMQMCQQEDIALP+LVAQVQGDPVLAGRLIQI Sbjct: 1 MSTYVDPQTFREMQDSEVLPAPSGVRLKIMQMCQQEDIALPELVAQVQGDPVLAGRLIQI 60 Query: 61 ANIPNINKGRMVAAVNVELLLMVGLHAVRQMALAISLTESARRGDCKEFDYDLFWSRSTA 120 ANIPNINKGRMVA+VNVELLLMVGLHAVRQMALAISLTESARRGDC EFDYD FWSRSTA Sbjct: 61 ANIPNINKGRMVASVNVELLLMVGLHAVRQMALAISLTESARRGDCAEFDYDAFWSRSTA 120 Query: 121 MACAGQALGDLLQVAPPAEMFTIGLLXRADIGRLGLASARPKRYGELLASGVQGSQLRQE 180 MACAGQALGDLLQVAPPAEMFTIGLL A IG+LGLASARPKRYGELL +G + +QLR + Sbjct: 121 MACAGQALGDLLQVAPPAEMFTIGLL--AGIGQLGLASARPKRYGELLLAGAKDAQLRVQ 178 Query: 181 EKALFGFNHLELAAAMMQDWHFPKLFCETVLYHHDADNTGMDEEDRQQRLIRLLELAACV 240 E+ FGF+HL LAAAMMQDWHFP++FCE VL+H D +G++E+DRQQ++ RLLELA CV Sbjct: 179 ERESFGFDHLALAAAMMQDWHFPRIFCEAVLHHRDPAASGLEEDDRQQKVTRLLELAGCV 238 Query: 241 ADVCVASDAARPALLPRLLALGERFELPLSSLEYLCGRVSWDWSDWGALLKVPTRVLREI 300 ADVC ASDA R ALLPRLL LG+ F LP+SSLEY+CGRVSWDWSDWGALLKVPTRVLREI Sbjct: 239 ADVCTASDAQRAALLPRLLQLGQHFALPVSSLEYVCGRVSWDWSDWGALLKVPTRVLREI 298 Query: 301 GTELAAAAEK 310 G ELAAAAE+ Sbjct: 299 GPELAAAAEQ 308 >gi|300310564|ref|YP_003774656.1| regulator of the two-component system protein [Herbaspirillum seropedicae SmR1] Length = 317 Score = 217 bits (552), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 117/292 (40%), Positives = 174/292 (59%), Gaps = 2/292 (0%) Query: 1 MSHYVDPKVFRELQDSEVLPAPSGVRLKIMQMCQQEDIALPDLVAQVQGDPVLAGRLIQI 60 M +++P +F +L+ ++ LP+P GVRL IM++C Q++ + +L+ VQ DP L+GR+I+I Sbjct: 1 MLTFIEPTLFDKLRSAQALPSPGGVRLTIMRLCAQDNPDMAELIHLVQADPALSGRIIRI 60 Query: 61 ANIPNINKGRMVAAVNVELLLMVGLHAVRQMALAISLTESARRGDCKEFDYDLFWSRSTA 120 N+ N + R +A+V+++ LL+VGL AVRQ+ALA+SL E+ C F FW+ S A Sbjct: 61 VNVLNAQRVRPIASVSMDSLLLVGLQAVRQLALALSLAETRAPASCANFSLPAFWAHSFA 120 Query: 121 MACAGQALGDLLQVAPPAEMFTIGLLXRADIGRLGLASARPKRYGELLASGVQGSQLRQE 180 MAC+ QA+ L+VAP EMFT GLL A+IG+L LASA+P Y LL + R Sbjct: 121 MACSAQAVTSQLRVAPLGEMFTTGLL--ANIGKLTLASAQPTGYSALLTRALDAPARRAM 178 Query: 181 EKALFGFNHLELAAAMMQDWHFPKLFCETVLYHHDADNTGMDEEDRQQRLIRLLELAACV 240 E++LFGFNHL +AAA+MQDW P LFC+ VL++ G RQ ++ L LA + Sbjct: 179 EQSLFGFNHLNIAAALMQDWKIPDLFCDAVLHYETPVVIGEIAATRQDEIVWTLHLAQAM 238 Query: 241 ADVCVASDAARPALLPRLLALGERFELPLSSLEYLCGRVSWDWSDWGALLKV 292 A++ A L R + +L L L + DW +W +++ Sbjct: 239 AELGCGPAQQAQAALARAGTALAQLQLAPHDLLVLYQQAGQDWQEWTQTMRI 290 >gi|300312232|ref|YP_003776324.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 60.1 bits (144), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 44/200 (22%), Positives = 98/200 (49%), Gaps = 9/200 (4%) Query: 13 LQDSEVLPAPSGVRLKIMQMCQQEDIALPDLVAQVQGDPVLAGRLIQIANIPNINKGRMV 72 Q LP+ V ++++ E +++ ++ ++ D VL+ +++++AN + R + Sbjct: 7 FQQQTALPSIPKVVQEVIESFNNESVSIEEIAKKLAADQVLSAKILRLANSSYYHVSRTI 66 Query: 73 AAVNVELLLMVGLHAVRQMALAISLTESARRGDCKEFDYDLFWSRSTAMACAGQALGDLL 132 V+ + +LM+G VR + ++ + S ++ D LFW S A + L Sbjct: 67 GTVD-DAVLMLGFMTVRTLVVSTGMAGSFKK--LPGVDLKLFWRYSLDTALVAKWLAKRG 123 Query: 133 QVAPPAEMFTIGLLXRADIGRLGLASARPKRYGEL--LASGVQGSQLRQEEKALFGFNHL 190 ++ FT+GL+ IG+L + + P++ ++ +A + +L E K+ FG++ Sbjct: 124 KLNSDF-AFTVGLMHA--IGQLVMHAGMPEQALQVDKVAGPLDARRLDVERKS-FGYDFA 179 Query: 191 ELAAAMMQDWHFPKLFCETV 210 ++ A + + W FP F + + Sbjct: 180 DVGAELARRWRFPDEFAKVI 199 >gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626 [Herbaspirillum seropedicae SmR1] Length = 290 Score = 58.9 bits (141), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 55/227 (24%), Positives = 99/227 (43%), Gaps = 11/227 (4%) Query: 19 LPAPSGVRLKIMQMCQQEDIALPDLVAQVQGDPVLAGRLIQIANIPNINKGRMVAAVNVE 78 LP V ++M EDI+ ++ Q+ DP L+ +++++AN + R + V+ + Sbjct: 13 LPTVPKVVQQLMASFNAEDISAHEIAQQIATDPALSAKVLRLANSAFFHASRTIGTVD-D 71 Query: 79 LLLMVGLHAVRQMALAISLTESARRGDCKEFDYDLFWSRSTAMACAGQAL---GDLLQVA 135 L ++G VR + L + + + + + D FW + A A + L ++ Sbjct: 72 ALRILGFIMVRNLVLGNGMVAAFK--NTRGMDLHQFWRHTLYTAVAARWLAMHAGMVGED 129 Query: 136 PPAEMFTIGLLXRADIGRLGLASARPKRYGEL-LASGVQGSQLRQEEKALFGFNHLELAA 194 +FT+G++ IG+L L + L S V S + EK +GF++ +++A Sbjct: 130 NGDAVFTVGMMH--GIGQLQLHAVAANAVAPLDRHSNVLDSDRAEREKEAWGFHYADVSA 187 Query: 195 AMMQDWHFPKLFCETVLYHHDADNTGMDEEDRQQRLIRLLELAACVA 241 + WHFP + D G DR + L AA VA Sbjct: 188 ELAAIWHFPDALVTAL--RSVPDPLGAGRFDRAAAWVHLGGWAARVA 232 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 867,840 Number of extensions: 32269 Number of successful extensions: 108 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 107 Number of HSP's successfully gapped: 4 Length of query: 312 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 227 Effective length of database: 1,217,395 Effective search space: 276348665 Effective search space used: 276348665 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)