BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0135.p (250 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311601|ref|YP_003775693.1| glutamine ABC transporter perip... 454 e-129 gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signa... 120 9e-29 gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/sign... 101 6e-23 gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/sign... 94 8e-21 gi|300310494|ref|YP_003774586.1| amino acid ABC transporter/sign... 90 1e-19 >gi|300311601|ref|YP_003775693.1| glutamine ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 251 Score = 454 bits (1167), Expect = e-129, Method: Compositional matrix adjust. Identities = 220/248 (88%), Positives = 230/248 (92%) Query: 3 LSRLLCAVSASLFLLVAAAPAARAADNVIRVGTDATFPPMEFVKDGKRTGFDIELMEALA 62 S+LLCA ASL LL+AA PAA A VIRVGTDATFPPMEFVKDGKRTGFD+EL+EALA Sbjct: 4 FSKLLCAGMASLSLLIAAVPAAHANGEVIRVGTDATFPPMEFVKDGKRTGFDVELIEALA 63 Query: 63 KTMGKKIQWIDIDFKGLIPGLISNRFDIAASAIYMTDERRKVVTFSDPYYRGGLAVLVRR 122 KTMGKK+QWIDIDFKGLIPGL+SNRFDIAASAIYMTDERRKVV FSDPYYRGGLAVLVRR Sbjct: 64 KTMGKKVQWIDIDFKGLIPGLVSNRFDIAASAIYMTDERRKVVNFSDPYYRGGLAVLVRR 123 Query: 123 DDNSIKVPEDLNKGKRVSVQVGTKSVSYLRDNFPGVERVEVEKNQAMFDLLATGRVNAVV 182 DDNSIKVPEDLNKGKRVSVQVGTKSV YL+DNFPGVERVEVEKNQAMFDLLATGRVNAVV Sbjct: 124 DDNSIKVPEDLNKGKRVSVQVGTKSVGYLKDNFPGVERVEVEKNQAMFDLLATGRVNAVV 183 Query: 183 TGRPAAVEYARTQPLVRVLDKGLTTELYGFALRKNDTALVEHLNKALQTLRMNGTYDALT 242 TGRPAAVEYARTQPLVRVLDKGLTTELYGFALRK+DT L LNKALQTLRMNGTY ALT Sbjct: 184 TGRPAAVEYARTQPLVRVLDKGLTTELYGFALRKSDTELTAQLNKALQTLRMNGTYTALT 243 Query: 243 DKWFARKD 250 DKWF + + Sbjct: 244 DKWFGKSE 251 >gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signal transduction system periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 120 bits (302), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 81/239 (33%), Positives = 121/239 (50%), Gaps = 7/239 (2%) Query: 10 VSASLFLLVAAAPAARAADNVIRVGTDATFPPMEFVKDGKRTGFDIELMEALAKTMGKKI 69 V ASL L +ARA + V D F P EF + GFDI+L +A+AK +G Sbjct: 9 VFASLVALSVVGASARA--ETLVVACDTAFVPFEFKDRNQYVGFDIDLWDAIAKDIGVTY 66 Query: 70 QWIDIDFKGLIPGLISNRFDIAASAIYMTDERRKVVTFSDPYYRGGLAVLVRRDDNSIKV 129 + +DF G+IP L + D+A + I + DER+KV+ FSD YY G +++V +SIK Sbjct: 67 KLQPMDFNGIIPALQTKNVDVALAGITIKDERKKVIDFSDGYYDSGFSLMVPV-GSSIKS 125 Query: 130 PEDLNKGKRVSVQVGTKSVSYLRDNFPGVERVEVEKNQAMFDLLATGRVNAVVTGRPAAV 189 DL GK ++V+ GT + Y + NF E + + L TGRV+A + P + Sbjct: 126 VADL-AGKALAVKSGTSAFDYAKANFKATELHQFPNIDNAYLELQTGRVDAAMHDTPNVL 184 Query: 190 EYART--QPLVRVLDKGLTTELYGFALRKNDTALVEHLNKALQTLRMNGTYDALTDKWF 246 Y T + +V+ + YG K + LV +N AL ++ +G Y + KWF Sbjct: 185 YYIATAGKGRAKVVGTQMMAHQYGIGFPKG-SPLVPKVNAALAKIKADGRYAEIYRKWF 242 >gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 271 Score = 101 bits (251), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 74/235 (31%), Positives = 117/235 (49%), Gaps = 27/235 (11%) Query: 30 VIRVGTDATFPPMEFVKDGKRT-GFDIELMEALAKTMGKKIQWIDIDFKGLIPGLISNRF 88 V VG ++ + P + K GFDI++M+ALAK +G +++++ F+G L Sbjct: 48 VYVVGVESAYAPFSSENEQKDVVGFDIDIMKALAKKIGIEVKFVPTPFEGFFNFLAQGDR 107 Query: 89 DIAASAIYMTDERRKVVTFSDPYYRGGLAVLVRRDDNSIKVPEDLNKGKRVSVQVGTKSV 148 D+ SAI +TDER+K V FS+PY+ + + D + EDL + VGT+S Sbjct: 108 DLLISAITITDERKKSVAFSEPYFVATQTIALPAADTKVSKMEDLK-----PLTVGTQSA 162 Query: 149 SYLRDNFPGVERVE--VEKNQAM---FDL-------LATGRVNAVVTGRPAAVEYARTQP 196 + G E V+ + KN A FD L +G V+AVV P Y P Sbjct: 163 T------SGDELVQQVLGKNSAKIKRFDSTPLALKELESGGVDAVVADEPVVKNYIANNP 216 Query: 197 ---LVRVLDKGLTTELYGFALRKNDTALVEHLNKALQTLRMNGTYDALTDKWFAR 248 L V D E YG A+RK+D L+ +NK L ++ +G++ A++ ++F + Sbjct: 217 NSKLRTVTDPSFPKEDYGIAVRKDDPELLAKINKGLAEMKADGSFAAISAQYFGK 271 >gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 267 Score = 94.4 bits (233), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 73/253 (28%), Positives = 127/253 (50%), Gaps = 15/253 (5%) Query: 6 LLCAVSASLF---LLVAAAPAARA--ADNVIRVGTDATFPPMEF--VKDGKRTGFDIELM 58 LL V A+L L V+AA ++ + ++V + +PP F K G+ TGF++++ Sbjct: 9 LLAGVGAALLASSLSVSAADLLQSVKSSGTLKVALEGNYPPFNFKDPKTGELTGFEVDVA 68 Query: 59 EALAKTMGKKIQWIDIDFKGLIPGLISNRFDIAASAIYMTDERRKVVTFSDPYYRGGLAV 118 + LA +G K + ++ G++ GL + ++D+ + + +TDER+K FSDPY + Sbjct: 69 KLLAAKLGVKPVFTTTEWSGILAGLGAGKYDVIINQVGITDERQKAFDFSDPYTLSSAQL 128 Query: 119 LVRRDDN-SIKVPEDLNKGKRVSVQVGTKSVSYLRDNFPGVERVEVEKNQAMFDLLATGR 177 +VR+D+ K EDL KGK++ + GT + PG++ + LA GR Sbjct: 129 IVRKDEKREFKSLEDL-KGKKLGLGQGT-NFEQKAKAVPGIDVRTYPGSPEYLADLAAGR 186 Query: 178 VNAVVTGRPAAVEY---ARTQPLVRVLDKGLTTELYGFALRKNDTALVEHLNKALQTLRM 234 ++A + V Y + PL G ++ G RK + LNKAL ++ Sbjct: 187 IDAALNDS-LLVGYILKSTNLPLKAGSPIGAVDKI-GIPFRKGNPEFKAALNKALADIKA 244 Query: 235 NGTYDALTDKWFA 247 +G++ A ++KWF Sbjct: 245 DGSFKAASEKWFG 257 >gi|300310494|ref|YP_003774586.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 258 Score = 90.1 bits (222), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 82/238 (34%), Positives = 117/238 (49%), Gaps = 18/238 (7%) Query: 19 AAAPA-ARAADNVIRVGTDATFPPMEF--VKDGKRTGFDIELMEALAKTMGKKIQWIDID 75 +APA A+A+ NV G +T P F +K K G ++ + A+ G + Sbjct: 25 GSAPAMAQASYNV---GATSTGVPFTFLDIKTNKLQGMMVDTVTAVGNKAGFSVNIQQTP 81 Query: 76 FKGLIPGLISNRFDIAASAIYMTDERRKVVTFSDPYYRGGLAVLVR-RDDNSIKVPEDLN 134 F LIP L N+ DI A+A+ T+ R ++V FSDP Y G + V +D S K +DL Sbjct: 82 FAALIPSLTGNKIDIIAAAMLKTEARAQIVDFSDPVYSYGEGLFVNAQDGKSYKNLDDL- 140 Query: 135 KGKRVSVQVGTKSVSYLRDNFPGVERVEVEKNQA-MFDLLATGRVNAVVTGRPAAVEYAR 193 KG+ V QVGT V L G + V+ + A M +A GR+ A RP V Y Sbjct: 141 KGEIVGAQVGTVFVDALNKK-GGFKEVKTYDSVADMMRDVALGRIKAGFGDRP-IVAYQL 198 Query: 194 TQ---PLVRVLDKGLTTELYG---FALRKNDTALVEHLNKALQTLRMNGTYDALTDKW 245 Q P VR++ K L G +RK D L+E LNKA+ L+ +GT + ++W Sbjct: 199 AQGANPQVRLV-KEYQPTLMGDVCLVIRKGDPKLMERLNKAIAELKADGTLARIVEQW 255 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 675,971 Number of extensions: 26687 Number of successful extensions: 109 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 93 Number of HSP's successfully gapped: 14 Length of query: 250 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 167 Effective length of database: 1,226,865 Effective search space: 204886455 Effective search space used: 204886455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)