BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0130.p (488 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311604|ref|YP_003775696.1| formiminoglutamate deiminase [H... 652 0.0 gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylam... 59 7e-10 gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hy... 57 3e-09 >gi|300311604|ref|YP_003775696.1| formiminoglutamate deiminase [Herbaspirillum seropedicae SmR1] Length = 482 Score = 652 bits (1681), Expect = 0.0, Method: Compositional matrix adjust. Identities = 349/488 (71%), Positives = 386/488 (79%), Gaps = 6/488 (1%) Query: 1 MASLGEQTQQHLHAHPQPHAHALPRQSARALFCPLALLPDGWQHDVLLRWDDSGRLIEVS 60 MAS G+Q HL P+ + ALFCPLALLP GW+ DVLLRWD +GRL V+ Sbjct: 1 MASHGDQDLHHLDQAPENAC------ANGALFCPLALLPQGWRSDVLLRWDGAGRLTHVT 54 Query: 61 VRSPRGEAAQASGPVIAGMPNLHSHAFQRAMAGLTEVMGSPSDSFWSWRKLMYRFAQRLQ 120 S RG AA ASGPV+ GMPNLHSHAFQRAMAGL E MGSP+DSFWSWR+LMYRFAQRLQ Sbjct: 55 PDSARGSAALASGPVLPGMPNLHSHAFQRAMAGLAETMGSPADSFWSWRELMYRFAQRLQ 114 Query: 121 PEHLHAIACHLYIEMLKAGYTSVCEFHYLHHAPGGSPYADRAELALRLMQAASEAGIGMT 180 P L AIA YIEMLKAGYTSVCEFHYLHHAP G+PYAD AE+A L+QAA ++GIGMT Sbjct: 115 PRQLEAIARQTYIEMLKAGYTSVCEFHYLHHAPDGAPYADPAEMAWPLLQAAGQSGIGMT 174 Query: 181 LLPVLYQYGGFGGQPGSQEQARFIATPDDLLALRERLLHALPENPMRRYGAAPHSLRAVS 240 LLPVLYQ GG GG+P S EQ RF+A P LL LRERLL +PEN MRRYG APHSLRAV+ Sbjct: 175 LLPVLYQQGGLGGRPVSAEQRRFVAAPSWLLGLRERLLQHMPENDMRRYGVAPHSLRAVA 234 Query: 241 PQGLEELVSGLRQQPGGRDAPVHIHVAEQWREIQDCLAWCGQRPVQLLLSLQEVDARWCL 300 P GLEEL+SGL QPGG DAP+HIHVAEQ RE++DCLAW GQRPVQ LLS Q VDARWCL Sbjct: 235 PTGLEELLSGLAGQPGGSDAPIHIHVAEQLREVEDCLAWSGQRPVQWLLSSQPVDARWCL 294 Query: 301 IHATHMDQGEYLDLAASGAIVGLCPTTEANLGDGIIDAGQLLQSRTPWGLGSDSNIAINP 360 +HATHMD EY +A SGA+VGLCPTTEANLGDG+IDA +L+ S WG+GSDSNIAI+ Sbjct: 295 VHATHMDDEEYRAVAGSGAVVGLCPTTEANLGDGVIDAPRLIDSAARWGIGSDSNIAISL 354 Query: 361 RSELRLLEYGQRLHHRRRNVLASEASPATADRLFAQAVAGGAAASGRAVAGLAVGQQADF 420 R+ELRLLEY QRLHHRRRNVLA +PA ADRLF QA+AGGA A+GRAVAGL GQ+AD Sbjct: 355 RAELRLLEYAQRLHHRRRNVLAVIDAPAVADRLFCQALAGGAQAAGRAVAGLMPGQRADL 414 Query: 421 VVLDGDDVNLADRSPGQLLSALVFCEHDGQPIQDVYVGGRQVVTAGQHPLQDDARRAYRR 480 VVLD D VNLA RS QLLSALVFCEHDG I DVYVGGRQVV G+H L D AR YR Sbjct: 415 VVLDPDHVNLAQRSSTQLLSALVFCEHDGPMIGDVYVGGRQVVKEGRHALDDRAREDYRH 474 Query: 481 AIAELLKD 488 A+A LLKD Sbjct: 475 ALAALLKD 482 >gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylaminohydrolase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 59.3 bits (142), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 111/439 (25%), Positives = 172/439 (39%), Gaps = 64/439 (14%) Query: 81 NLHSHAFQRAMAGLTEVMGSPSDSFWSWRKLM-YRFAQRLQPEHLHAIACHLYIEMLKAG 139 N H H FQ + G + +G + + W YR+ + A +E++++G Sbjct: 63 NTHHHLFQSLLKG--DPLGINA-TLTPWLAATPYRYRASFDADLFRLAARIGMVELMRSG 119 Query: 140 YTSVCEFHYLHHAPGGSPYADRAELALRLMQAASEAGIGMTLLPVLYQYGGFGGQPGSQE 199 ++ + +YL++ G P+ D +E+ L + A + G+ L G+ Sbjct: 120 CGTIADHNYLYYP--GMPF-DTSEI---LFEEAQQLGLRFVLCR------------GTAT 161 Query: 200 QARFIA----------TPDDLLALRERL---LHALPENPMRRYGAAPHS-LRAVSPQGLE 245 + R + T D LA + L H MRR AP + L + +P L Sbjct: 162 RTRQLEAELSSALRPETLDGFLADMQGLAGRFHDPAPASMRRVVMAPTTPLYSAAPDELR 221 Query: 246 ELVSGLRQQPGGRDAPVHIHVAEQWREIQDCLAWCGQRPVQLLLSLQEVDARWCLIHATH 305 E+ R+ G R +H H++E A G RPV+ + + H Sbjct: 222 EMAVAAREM-GLR---LHSHLSETVGYQDSAHAMHGCRPVEFCERVGWLGRDVWFAHLVK 277 Query: 306 MDQGEYLDLAASGAIVGLCPTTEANLGDGIIDAGQLLQSRTPWGLGSD---SNIAINPRS 362 +D E L +G + CP + LG GI L + P +G D SN A + S Sbjct: 278 LDAEEIQLLGGTGTGIAHCPQSNGRLGSGIAPIRALEAAGVPVSIGVDGAASNEAADMIS 337 Query: 363 ELRLLEYGQRLHH-------RRRNVLASEASPATADRLFAQAVAGGAAASG-RAVAGLAV 414 E QR R A AT + + GGA G A+ GL V Sbjct: 338 ETHAAWLMQRARRGQEATPSFRGGHFEGGADAATVEDVVRWGTTGGARVLGLDALDGLQV 397 Query: 415 GQQADFVVLDGDD---VNLADRSPGQLLSALVFCEHDGQP-IQDVYVGGRQVVTAGQHPL 470 GQQAD + DD L D + G + S G+P ++ ++VGGR VV P Sbjct: 398 GQQADIALYRLDDPRYFGLHDPAIGPVASG-------GRPFLKAMWVGGRAVVREDAIPG 450 Query: 471 QDDARRA--YRRAIAELLK 487 D A R+A+ ++LK Sbjct: 451 VDLAELGAQARQAVKKMLK 469 >gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hydrolase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 57.4 bits (137), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 99/409 (24%), Positives = 152/409 (37%), Gaps = 55/409 (13%) Query: 75 VIAGMPNLHSHAFQRAMAGLTEVMGSPSD-SFWSWRKLMYRFAQRLQPEHLHAIACHLYI 133 V+ G+ N H H +Q LT + S + ++W +Y L PE + Sbjct: 64 VLPGLINTHHHMYQ----SLTRAIPSAQNGELFNWLTKLYPIWAGLTPEMIQVSTLTAMA 119 Query: 134 EMLKAGYTSVCEFHYLHHAPGGSPYADRAELALRLMQAASEAGIGMTLLPVLYQYGGFGG 193 E++ +G T+ + Y++ P + D E A R IGM +G G Sbjct: 120 ELILSGCTTSSDHLYIY--PNQTRLDDSLEAAAR---------IGMRF------HGARGA 162 Query: 194 QPGSQEQA-----RFIATPDDLLALRERLL---HALPENPMRRYGAAPHSLRAVSPQGLE 245 Q + R + +L +RL+ H + M+R AP S +VS + + Sbjct: 163 MSVGQSKGGLPPDRVVQDEAAILKDTQRLIETYHDHQRHAMQRIVVAPCSPFSVSRELMR 222 Query: 246 ELVSGLRQQPGGRDAPVHIHVAEQWREIQDCLAWCGQRPVQLLLSLQEVDARWCLIHATH 305 E + L + G +H H+AE +I P + V H Sbjct: 223 E-AADLARHYG---VSLHTHLAENVNDIAYSREKFNMSPAEYAEDCGWVGHDVWHAHCVQ 278 Query: 306 MDQGEYLDLAASGAIVGLCPTTEANLGDGIIDAGQLLQSRTPWGLGSD---SNIAINPRS 362 +D A +G V CP + L GI ++L + P GLG D SN + + Sbjct: 279 LDAHGIDLFARTGTGVAHCPCSNMRLASGIAPIRKMLDAGVPVGLGVDGSASNDSAHMLG 338 Query: 363 ELRLLEYGQRLHHRRRNVLASEASPATADRLFAQAVAGGAAASGR-AVAGLAVGQQADFV 421 E+R QR + TA + A GGA R + L G AD V Sbjct: 339 EVRQAMLLQR--------VGFGPDAMTARQALEVATLGGAKVLNRDDIGALKPGMSADVV 390 Query: 422 VLDGDDVNLADRSPGQL---LSALVFCEHDGQPIQDVYVGGRQVVTAGQ 467 + D + A G L ++ALVFC + + GR +V GQ Sbjct: 391 MFDTRQIAFA----GALHDPVAALVFCT--PANVDCSIINGRVIVREGQ 433 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,474,695 Number of extensions: 63903 Number of successful extensions: 212 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 213 Number of HSP's successfully gapped: 3 Length of query: 488 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 399 Effective length of database: 1,198,455 Effective search space: 478183545 Effective search space used: 478183545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)