BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0118.p (681 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311615|ref|YP_003775707.1| outer membrane biogenesis prote... 795 0.0 gi|300314078|ref|YP_003778170.1| uncharacterized protein involve... 390 e-109 >gi|300311615|ref|YP_003775707.1| outer membrane biogenesis protein [Herbaspirillum seropedicae SmR1] Length = 654 Score = 795 bits (2054), Expect = 0.0, Method: Compositional matrix adjust. Identities = 420/637 (65%), Positives = 502/637 (78%), Gaps = 26/637 (4%) Query: 42 DWNLAKPWITRQLSAVLAREIRVDGPITLGWSLAPAHKDGSS--PWLPQLHFTARELHIG 99 DWN AKPW+ RQLSA L R+IRVDG + L WSLA A + + PQL A L IG Sbjct: 41 DWNSAKPWLGRQLSAALGRDIRVDGNLDLSWSLAQADDEAGALARLAPQLRLRAERLFIG 100 Query: 100 NPDWARSADHLLRARTLDLHFSPLPLLRWHWHITDLRVEGLDLVMERADERRKSWRFSDQ 159 NPDWA S+DHL+RA LD+ FSPLPLL WHWH+TDLRVEGLDLVMERAD++RK+WRFS Q Sbjct: 101 NPDWAHSSDHLVRAAALDVRFSPLPLLHWHWHVTDLRVEGLDLVMERADDQRKNWRFSSQ 160 Query: 160 PRSRWSFDIHQIVFERAQIRYVDQPLNMDLHFDVRPLVSSANQAAEVRQAEGAASVGPAN 219 PRSRWSFDIH+IVF A IRYV+ PL++DL FD RPL ++A + Sbjct: 161 PRSRWSFDIHRIVFGHALIRYVELPLDLDLRFDARPLNATATNQKD-------------- 206 Query: 220 PSAMVLQAAITGRFGDASIQGNLRGGALPDLLNEDSIYPLQAEGEVGEVHTALSGKLINP 279 +A+VLQAA+TGR+G A + G ++GGALPDLLNE S++PLQAEG++G V T LSG LINP Sbjct: 207 SAALVLQAAVTGRYGQAEVNGQIKGGALPDLLNEGSVFPLQAEGDIGGVQTQLSGNLINP 266 Query: 280 HQLTRAELQLQLSGDSLDRLYAASGVPLPATRPFTTQGQLSITRLDTETRAWDWRYAHFA 339 HQL+RA+L++ L+GDSLDRLY A+GVPLPATR F T+G L+ITRLD +T+AWDWRY+ F+ Sbjct: 267 HQLSRADLRIGLAGDSLDRLYPATGVPLPATRAFHTEGALTITRLDPQTKAWDWRYSGFS 326 Query: 340 GKVGDSDFSGDAQYYRGPPKNRLRVTAEAGLLRLSDYVPDAGKKSGVHPDKTATRVLPDQ 399 GKVG+SDF+GDAQY RGPPKN L+V AEAGLLRLSDY+P G P + Sbjct: 327 GKVGESDFAGDAQYVRGPPKNHLKVKAEAGLLRLSDYLPGDDADQGK----------PSK 376 Query: 400 PAKPQRWAKLDAEIALHAKRVQLHPDIALQDASTTLRLQDQVLTAAPLHFALAGGKGEGE 459 PA+P+RW+KLDA+I L AKRVQ + Q A+ TL L+DQVLTA PL F +AGG+G+G+ Sbjct: 377 PAQPERWSKLDADIDLQAKRVQWRAGLEWQQAAATLTLKDQVLTADPLRFNMAGGQGQGQ 436 Query: 460 IRLDGRQTDIGAKLQLQVQGVQVRELFPRLARIDASFGKVDGRATLTGSGNSVAAMLAHA 519 +RLDGRQ DI ++LQ+++Q QVRELFPRLA + ASF K +G ATL+ +GNS AA+LA A Sbjct: 437 VRLDGRQPDIRSRLQIRLQDAQVRELFPRLASLKASFSKFNGEATLSATGNSAAAVLASA 496 Query: 520 NGEIKADLSQGTISQFILEAAGLNLANAVFAKLYRDQQVKLLCGAADVGIKDGLAQVRHG 579 +GEIKADL GTISQFILEAAGLNLANAVFAKLYRDQQVKLLCGAADV I+ G A +RHG Sbjct: 497 DGEIKADLGPGTISQFILEAAGLNLANAVFAKLYRDQQVKLLCGAADVAIQHGQATIRHG 556 Query: 580 ILNTEDAAIDISGQVDLSKETLSLDVVPRTKQVRILSLRTPLYVRGTFAKPDIGASKGAL 639 ILNTEDAAID+ GQVDL ++TL+LD+ PRTKQVRILSLRTPL+VRG+F+ P+IGA GAL Sbjct: 557 ILNTEDAAIDVGGQVDLGRQTLALDIHPRTKQVRILSLRTPLHVRGSFSHPEIGADSGAL 616 Query: 640 AARAGAAAALALVAPVAAVIPLITPGQKIPDDCAPHA 676 AARAGAAAALALVAPVAAVIPLI+PGQ DDCA A Sbjct: 617 AARAGAAAALALVAPVAAVIPLISPGQAASDDCAARA 653 >gi|300314078|ref|YP_003778170.1| uncharacterized protein involved in outer membrane biogenesis protein [Herbaspirillum seropedicae SmR1] Length = 682 Score = 390 bits (1003), Expect = e-109, Method: Compositional matrix adjust. Identities = 240/677 (35%), Positives = 358/677 (52%), Gaps = 38/677 (5%) Query: 13 MRRGFRFLTWVALTLVALMATLALAVALFDWNLAKPWITRQLSAVLAREIRVDGPITLGW 72 M R + + W+ +L+ L+ + + FDWN AKPWI ++SA + RE +DG ++L W Sbjct: 1 MPRLTKIILWLCASLILLITAAVIFILSFDWNRAKPWINERVSASIGREFAIDGDLSLHW 60 Query: 73 SLAPAHKDGSSP-----WLPQLHFTARELHIGNPDWARSADHLLRARTLDLHFSPLPLLR 127 A + P WLP A +L IGNP A + + +PLPLL Sbjct: 61 QRAQTLPGAAPPGFWERWLPWPRLQAEQLRIGNPPAFSQAGQMATIGQVTFTLNPLPLLV 120 Query: 128 WHWHITDLRVEGLDLVMERADERRKSWRF--SDQPRSRWSFDIHQIVFERAQIRYVDQPL 185 + L + + + R + +W F S S W+ + +I + ++ D Sbjct: 121 HDIALPSLDIRDPSVSLLRNAQAVNNWTFKSSSAGPSAWNLRLGRITLRQGKLSLDDAVD 180 Query: 186 NMDLHFDVRPLVSSANQAAEVRQAEGAASVGPANPSAMVLQAAITGRFGDASIQGNLRGG 245 + L ++ L PA L ++G F A + G + G Sbjct: 181 KIKLKAEIDTL-------------------DPAVEKIYGLGWKLSGSFNGAPVSGQGKAG 221 Query: 246 ALPDLLNEDSIYPLQAEGEVGEVHTALSGKLINPHQLTRAELQLQLSGDSLDRLYAASGV 305 + L N YPLQA+ VG+ + G L P L +L+L +SG S+ LY +GV Sbjct: 222 GVLSLQNVQQPYPLQAQVRVGKTAIGVHGTLTRPAALAALDLRLSVSGASMGNLYPLTGV 281 Query: 306 PLPATRPFTTQGQLSITRLDTETRAWDWRYAHFAGKVGDSDFSGDAQYYRGPPKNRLRVT 365 LP T PF T+G+L I ++D WD Y F GKVG SD +G +Y G R ++T Sbjct: 282 LLPETPPFATEGRL-IGKIDAGHSQWD--YEDFRGKVGQSDINGSLRYTTGAGAARSQLT 338 Query: 366 AE--AGLLRLSDYVPDAGKKS-------GVHPDKTATRVLPDQPAKPQRWAKLDAEIALH 416 + +LR D P G S G + +VLP + W LDA++ L Sbjct: 339 GSLTSEVLRFDDLAPLIGADSNAEKAERGAQERQPGDKVLPVKTFSTDAWGALDADVKLT 398 Query: 417 AKRVQLHPDIALQDASTTLRLQDQVLTAAPLHFALAGGKGEGEIRLDGRQTDIGAKLQLQ 476 KR+ ++ + D L L+D+VLT APL F +AGG IRLDG+ + ++L L Sbjct: 399 GKRIIKQKNLPISDLQAHLILKDKVLTLAPLDFGVAGGNLRSTIRLDGQAAPMKSELILS 458 Query: 477 VQGVQVRELFPRLARIDASFGKVDGRATLTGSGNSVAAMLAHANGEIKADLSQGTISQFI 536 + +++++L+P+L SFG+++G A L+ +GNSVAAML +NGE+K ++QG IS+F+ Sbjct: 459 ARRLKIQKLYPQLDASRTSFGEINGDARLSATGNSVAAMLGSSNGEVKTLVTQGAISKFL 518 Query: 537 LEAAGLNLANAVFAKLYRDQQVKLLCGAADVGIKDGLAQVRHGILNTEDAAIDISGQVDL 596 LEAAGLNL N V +KL+ D++VKL C A D+ +++G+ R +L+TEDA I + G +D+ Sbjct: 519 LEAAGLNLGNVVISKLFGDKEVKLNCLAGDLPVREGIMTARTLMLDTEDALISVDGTIDM 578 Query: 597 SKETLSLDVVPRTKQVRILSLRTPLYVRGTFAKPDIGASKGALAARAGAAAALALVAPVA 656 +E L L V P++K +RI+SLR+PLYV GTF +PD+G KG LA RAG+A AL L+APV Sbjct: 579 RREKLDLHVHPKSKGLRIISLRSPLYVAGTFKQPDVGVDKGMLALRAGSAVALGLLAPVT 638 Query: 657 AVIPLITPGQKIPDDCA 673 A++PLI DCA Sbjct: 639 AILPLINVDGDEQADCA 655 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,097,666 Number of extensions: 91331 Number of successful extensions: 311 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 304 Number of HSP's successfully gapped: 2 Length of query: 681 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 589 Effective length of database: 1,184,250 Effective search space: 697523250 Effective search space used: 697523250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)