BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0115.p (677 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine... 1224 0.0 gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine k... 527 e-150 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 382 e-107 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 285 9e-78 gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 135 1e-32 >gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine kinase response regulator protein [Herbaspirillum seropedicae SmR1] Length = 688 Score = 1224 bits (3167), Expect = 0.0, Method: Compositional matrix adjust. Identities = 599/671 (89%), Positives = 634/671 (94%) Query: 1 LSRAYRHALLEAIDDGFCIIEFIDGPHGPLSDYVHIEANSGYYRHTGIEGIVGKRLREIE 60 LS AYRHALLEAIDDGFCIIEFIDGPHGPLSDYVHIEANSGY+RHTGIEGIVGKRLRE+E Sbjct: 13 LSSAYRHALLEAIDDGFCIIEFIDGPHGPLSDYVHIEANSGYHRHTGIEGIVGKRLREVE 72 Query: 61 PDNAQVWLDVYGQVLLTGQPLRFEQEFQAVGRHIEVSATRVGPVEMRQVSVLFRDITVRR 120 PDNAQVWLD+YG+VLLTGQ +RFEQEF+A RHIEVSATRVGPVEMRQVSVLFRD+T RR Sbjct: 73 PDNAQVWLDIYGKVLLTGQTVRFEQEFKAAARHIEVSATRVGPVEMRQVSVLFRDVTARR 132 Query: 121 RTEAALRENVQRVQLAMEAGAIIGTWIWDIPSDRFSVDDGFAAAFGVPPEKGLAGLKLED 180 RTEAALRENV+RVQLA+EAGAIIGTWIWD+PSDRFSVDDGFAAAFG+P E+GL G+KL++ Sbjct: 133 RTEAALRENVERVQLALEAGAIIGTWIWDLPSDRFSVDDGFAAAFGIPAERGLDGIKLDE 192 Query: 181 LMQSVHPDDRELLSTRIREALARGGAYAHQYRTRRRDGRYYWLEANGRVELTSDGHASTF 240 LM SVHPDDRELLS RIREALARGGAYAHQYRTRRRDGRYYWLEANGRVE+T DG S F Sbjct: 193 LMLSVHPDDRELLSARIREALARGGAYAHQYRTRRRDGRYYWLEANGRVEMTPDGIPSKF 252 Query: 241 PGVLIDIEGRRAVEAERDRALAALRALNDTLEQRVQERTTALLEAEEALRQAQKMEAVGQ 300 PGVLIDIEGRRAVEAERDRALAALR LN+TLEQRV+ERT ALL AEEALRQAQKMEAVGQ Sbjct: 253 PGVLIDIEGRRAVEAERDRALAALRTLNETLEQRVEERTAALLNAEEALRQAQKMEAVGQ 312 Query: 301 LTGGLAHDFNNILAGISGSLELMKVRLAQGRISDIERHLNGAQSAVKRAASLTQRLLAFS 360 LTGGLAHDFNNILAGISGSLELMKVRLAQGRI DIERHLNGAQSAVKRAA+LTQRLLAFS Sbjct: 313 LTGGLAHDFNNILAGISGSLELMKVRLAQGRIGDIERHLNGAQSAVKRAAALTQRLLAFS 372 Query: 361 RRQTLDPKSADLNRIVGDMHELIGRSVGPAITVETIAAGGLWNTFVDVGQLENALLNLCI 420 RRQTLDPK ADLNRIV MHELIGRSVGPAI VETIAAGGLW+TFVD+GQ+ENALLNLCI Sbjct: 373 RRQTLDPKPADLNRIVAGMHELIGRSVGPAIAVETIAAGGLWHTFVDIGQMENALLNLCI 432 Query: 421 NARDAMPHGGRLTIETANRWLDDIAAMQRGVPPGQYVSLCVSDTGTGMSPDVVARAFDPF 480 NARDAMPHGGRLTIETANRWLDDI A+QRGVPPGQYVSL VSDTG GMSP+VVARAFDPF Sbjct: 433 NARDAMPHGGRLTIETANRWLDDIGALQRGVPPGQYVSLSVSDTGAGMSPEVVARAFDPF 492 Query: 481 FTTKPTGQGTGLGLSMVYGFAGQSGGAVRIYSELGQGATLCIYLPRHLGEAGEEDLPLPD 540 FTTKPTGQGTGLGLSMVYGFAGQSGG VRIYSELGQGA +CIYLPRH+GE G +DL LP+ Sbjct: 493 FTTKPTGQGTGLGLSMVYGFAGQSGGTVRIYSELGQGAMVCIYLPRHVGEGGVDDLALPE 552 Query: 541 DGSAAAAPGSKTILVVDDEPLVRMVTVEVLSDLGYSVLEAEDGPSALRVLAAYPELDLLV 600 + AAPGSKTILVVDDEPLVRMVTVEVL DLGYSVLEAEDGPSALRV+ A E+DLL+ Sbjct: 553 ESHVPAAPGSKTILVVDDEPLVRMVTVEVLRDLGYSVLEAEDGPSALRVVEACQEIDLLL 612 Query: 601 TDVGLPNGMNGRQLADAIRAPRPELPVLFVTGYAENAVLNHGHLERGMQVLTKPFAADVL 660 TDVGLPNGMNGRQLADAIRAPRP+LPVLFVTGYAENAVLNHGHLERGMQVLTKPFAADVL Sbjct: 613 TDVGLPNGMNGRQLADAIRAPRPDLPVLFVTGYAENAVLNHGHLERGMQVLTKPFAADVL 672 Query: 661 ARRVRELVGEE 671 ARRV+ELVGE+ Sbjct: 673 ARRVKELVGED 683 >gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 845 Score = 527 bits (1358), Expect = e-150, Method: Compositional matrix adjust. Identities = 260/407 (63%), Positives = 320/407 (78%) Query: 262 AALRALNDTLEQRVQERTTALLEAEEALRQAQKMEAVGQLTGGLAHDFNNILAGISGSLE 321 A LRA+N++LE V RT L+ EEALR +QK+EA+GQLTGGLAHDFNN+L GISGSL+ Sbjct: 432 ARLRAINESLEAEVSARTRELMATEEALRHSQKIEAIGQLTGGLAHDFNNLLGGISGSLD 491 Query: 322 LMKVRLAQGRISDIERHLNGAQSAVKRAASLTQRLLAFSRRQTLDPKSADLNRIVGDMHE 381 LM+VRL QGR ++ R++ A + +RAASLTQRLLAF+RRQTL PK +N ++ M E Sbjct: 492 LMRVRLVQGRGEELLRYVELAARSTQRAASLTQRLLAFARRQTLAPKPTRVNALISGMEE 551 Query: 382 LIGRSVGPAITVETIAAGGLWNTFVDVGQLENALLNLCINARDAMPHGGRLTIETANRWL 441 LI R++GPAI +E + AGGLW TFVD QLE+ALLNLCINARDAMP GGRLTIETAN+WL Sbjct: 552 LIRRTIGPAIVLEVVGAGGLWPTFVDGPQLESALLNLCINARDAMPGGGRLTIETANKWL 611 Query: 442 DDIAAMQRGVPPGQYVSLCVSDTGTGMSPDVVARAFDPFFTTKPTGQGTGLGLSMVYGFA 501 DD A +R +PPGQYV + V+D+G GM PDV+ARAFDPFFTTKP G GTGLGLSMVYGFA Sbjct: 612 DDRWAHERDLPPGQYVCISVTDSGVGMPPDVLARAFDPFFTTKPIGAGTGLGLSMVYGFA 671 Query: 502 GQSGGAVRIYSELGQGATLCIYLPRHLGEAGEEDLPLPDDGSAAAAPGSKTILVVDDEPL 561 QSGG VR+YSE+GQG T+ IYLPRH + E+D D +AA + +LVVDDE Sbjct: 672 RQSGGQVRLYSEVGQGTTVSIYLPRHQHDGDEQDEADALDETAAGDGNGEVVLVVDDEAS 731 Query: 562 VRMVTVEVLSDLGYSVLEAEDGPSALRVLAAYPELDLLVTDVGLPNGMNGRQLADAIRAP 621 +RM+ E+LSD GY VL+AEDGP ALR+L + +DLL+TDVGLP G+NGRQ+ADA RA Sbjct: 732 IRMLVAEILSDAGYDVLQAEDGPGALRILQSKQRIDLLITDVGLPGGLNGRQVADAGRAA 791 Query: 622 RPELPVLFVTGYAENAVLNHGHLERGMQVLTKPFAADVLARRVRELV 668 RP+L VLF+TGYAENA ++GHLERGMQ++TKPF + LARRV+ ++ Sbjct: 792 RPDLHVLFITGYAENAAFDNGHLERGMQLMTKPFKMEALARRVQSIL 838 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 382 bits (981), Expect = e-107, Method: Compositional matrix adjust. Identities = 225/562 (40%), Positives = 334/562 (59%), Gaps = 21/562 (3%) Query: 113 FRDITVRRRTEAALRENVQRVQLAMEAGAIIGTWIWDIPSDRFSVDDGFAAAFGVPPEKG 172 F D+T RRR AL + R++ ++ + + D+ V+ G E+ Sbjct: 553 FVDLTERRRGLQALSWSENRLRAIFNQASVCFSEL-DLQGRYLRVNQALCRMLGRSEEEM 611 Query: 173 LAGLKLEDLMQSVHPDDRELLSTRIREALARGGAYAHQYRTRRRDGRYYWLEANGRVELT 232 L G+ L D+ HPDD + + + G ++ + R R DG +W+ ++ + Sbjct: 612 L-GMTLADV---THPDDVPMNMSLFNRCIEDGRSFTLEKRYLRPDGSTFWVSSDISRIVD 667 Query: 233 SDGHASTFPGVLIDIEGRRAVEAERDRALAALRALNDTLEQRVQERTTALLEAEEALRQA 292 +G V DI ER RA ALR LNDTLEQRV E AE ALRQ Sbjct: 668 GEGRPQAMIAVSTDI-------TERRRAEEALRELNDTLEQRVVEEILERSRAEAALRQM 720 Query: 293 QKMEAVGQLTGGLAHDFNNILAGISGSLELMKVRLAQGRISDIERH-LNGAQSAVKRAAS 351 QKMEAVGQLTGG+AHDFNN+L I G+L+L++ + R+ + R + A AV R A Sbjct: 721 QKMEAVGQLTGGVAHDFNNVLQIIGGNLQLLQ---SDPRLDERSRQRVRIAIGAVDRGAK 777 Query: 352 LTQRLLAFSRRQTLDPKSADLNRIVGDMHELIGRSVGPAITVETIAAGGLWNTFVDVGQL 411 L+ +LLAF+RRQ L P+S +L R+V +M +L+ R+VG I + T A LWNT +D Q+ Sbjct: 778 LSSQLLAFARRQPLQPRSINLERVVQNMDDLLRRAVGENIDISTRVAHDLWNTMLDPNQV 837 Query: 412 ENALLNLCINARDAMPHGGRLTIETANRWLDD-IAAMQRGVPPGQYVSLCVSDTGTGMSP 470 EN ++N INARDAMP GGRLT++ N L+D + Q + PG+YV L +SD G GMSP Sbjct: 838 ENLIINTAINARDAMPSGGRLTVQIDNVRLEDPVFLSQADLAPGEYVELQISDNGAGMSP 897 Query: 471 DVVARAFDPFFTTKPTGQGTGLGLSMVYGFAGQSGGAVRIYSELGQGATLCIYLPRHLGE 530 +V RAF+PFFTTK G+GTGLGLSM YGF QS G + I+SE G+G T+ IY PR L Sbjct: 898 EVRDRAFEPFFTTKLEGEGTGLGLSMAYGFVKQSKGHISIHSEQGKGTTIRIYFPRCL-- 955 Query: 531 AGEEDLPLPDDGSAAAAPGSKTILVVDDEPLVRMVTVEVLSDLGYSVLEAEDGPSALRVL 590 +E++ + + G +TILVV+D+P +++ ++ LS LGY VL+A++G AL +L Sbjct: 956 --DEEVRIDEVRDLTVHGGDETILVVEDDPNLQLTVIDTLSSLGYRVLKADNGERALEIL 1013 Query: 591 AAYPELDLLVTDVGLPNGMNGRQLADAIRAPRPELPVLFVTGYAENAVLNHGHLERGMQV 650 + +DL+ TDV +P + +LA R P++ +LF +GY + +++ G L+ G+++ Sbjct: 1014 QSGVAIDLVFTDVVMPGSVPVTELAQQARLLVPDIEILFTSGYPRDTIVHEGRLDAGVEL 1073 Query: 651 LTKPFAADVLARRVRELVGEES 672 L+KP+ + LA+R+R+++ + + Sbjct: 1074 LSKPYRINQLAQRIRQMLSQRA 1095 Score = 57.0 bits (136), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 47/153 (30%), Positives = 73/153 (47%), Gaps = 7/153 (4%) Query: 122 TEAALRENVQRVQLAMEAGAIIGTWIWDIPSDRFSVDDGFAAAFGVPPEKGLAGLKLEDL 181 TE L E +R+++ AG I G + W S R SV D F +G+PPE + +L + Sbjct: 299 TERMLAE--ERLRIVDRAGEI-GIFEWFPDSGRLSVSDRFRRLWGLPPEGEVTAAQLVGM 355 Query: 182 M-QSVHPDDRELLSTRIREALARGGAYAHQYRTRRRDGRYYWLEANGRVELTSDGHASTF 240 + + HP+ L + RI+E +R +Y R DG+ WL G V S + Sbjct: 356 IDEQFHPN---LGTARIKEGGSRNPLEYTEYHITRPDGQQRWLARKGEVVQDDPRTGSRY 412 Query: 241 PGVLIDIEGRRAVEAERDRALAALRALNDTLEQ 273 GV D+ R+ +E + A++RA N + Q Sbjct: 413 VGVAFDVTARKLMEQALAASEASVRANNAFVRQ 445 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 285 bits (729), Expect = 9e-78, Method: Compositional matrix adjust. Identities = 170/385 (44%), Positives = 237/385 (61%), Gaps = 7/385 (1%) Query: 284 EAEEALRQAQKMEAVGQLTGGLAHDFNNILAGISGSLELMKVRLAQGR--ISDIERHLNG 341 +AE+AL QA+KMEA+GQLTGG+AHDFNN+L I+G L+++ + L + ++ + R + Sbjct: 162 DAEQALGQARKMEALGQLTGGIAHDFNNLLQVITGYLDMINLALRSDKPDLARVARSADS 221 Query: 342 AQSAVKRAASLTQRLLAFSRRQTLDPKSADLNRIVGDMHELIGRSVGPAITVETIAAGGL 401 + A +AA LTQ+LLAF+R+Q L+ ++ +LN + +L+ R++G ITV+T A GL Sbjct: 222 IRKASGKAAMLTQQLLAFARKQRLEGRTINLNSLTEGFTDLVSRTLGGDITVQTELARGL 281 Query: 402 WNTFVDVGQLENALLNLCINARDAMPHGGRLTIETANRWL-DDIAAMQRGVPPGQYVSLC 460 WN +D Q+E ALLN+ INARDAM GR+ I TAN L D A+Q G+ G YVS+ Sbjct: 282 WNCRLDPTQMEVALLNVLINARDAMNGRGRVRIRTANEDLADPDRAVQLGLKEGLYVSIA 341 Query: 461 VSDTGTGMSPDVVARAFDPFFTTKPTGQGTGLGLSMVYGFAGQSGGAVRIYSELGQGATL 520 V+D G G++PD++ R DPFFTTK G+GTGLGLSMVYGF QSGG+VRI S G T+ Sbjct: 342 VTDDGPGIAPDILPRVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTV 401 Query: 521 CIYLPRHLGEAGEEDLPLPDDGSAAAAPGSKTILVVDDEPLVRMVTVEVLSDLGYSVLEA 580 IY P + G D + + G + ILVVDD V + +L LGY V Sbjct: 402 TIYFPATQDQVGG---GFEYDNRSQSQGGQEHILVVDDRLEVAELAQTMLESLGYRVSMV 458 Query: 581 EDGPSALRVLAAYPELDLLVTDVGLPNGMNGRQLADAIRAPRPELPVLFVTGYAENAVLN 640 AL+++ + +DLL TD+ +P MNG LA + P + VL TGYA ++ Sbjct: 459 NSPAEALQLIRSGRPVDLLFTDLIMPGSMNGVVLAREAKRMLPTIKVLLTTGYASESLER 518 Query: 641 HGHLERGMQVLTKPFAADVLARRVR 665 HG + VL KP+ D LAR++R Sbjct: 519 HGA-DGEFPVLDKPYRHDELARKIR 542 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 135 bits (339), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 102/379 (26%), Positives = 166/379 (43%), Gaps = 68/379 (17%) Query: 185 VHPDDRELLSTRIREALARGGAYAHQYRTRRRDGRYYWLEANGRVELTSDGHASTFPGVL 244 +HPDD + + + ++YR R +G Y W+ R+ DG G++ Sbjct: 86 IHPDDTAQIFSSLAMLFVEDQKI-YEYRFRTSNGSYLWMHDTLRLIRDPDGTPQEVIGLM 144 Query: 245 IDIEGRRAVEAERDRALAALRALNDTLEQRVQER---TTALLEAEEALRQAQKMEAVGQL 301 DI GR+ +E DTL+++ +E+ L A+ L Q++K+ ++GQL Sbjct: 145 TDITGRKEME--------------DTLQRQAEEQRKLIEQLKMAQAQLMQSEKLASIGQL 190 Query: 302 TGGLAHDFNNILAGISGSLELM----------------------KVRLAQGRI------- 332 G+AH+ NN + +S +L + +AQ ++ Sbjct: 191 AAGVAHEINNPIGFVSANLNTLGNYVATLLEGIQLHQCLQGGALPAAMAQEQLRSFEARA 250 Query: 333 ------SDIERHLNGAQSAVKRAASLTQRLLAFSRRQTLDPKSADLNRIVGDMHELIGRS 386 D+ L + ++R ++ Q L FSR + + DL+R + + Sbjct: 251 DLAFLSEDVTELLAESNEGLQRVRNIVQSLKDFSRIGESNWQMTDLHRGLDSTLNIANNE 310 Query: 387 VGPAITVETIAAGGLWNTFVDVGQLENALLNLCINARDAMPHGGRLTIETANRWLDDIAA 446 + +++ G L +L +NL +NA A+ G +TI TA Sbjct: 311 LKYKVSI-VKQYGELPLVKCLASELNQVFMNLLVNAAQAISERGTVTICTAR-------- 361 Query: 447 MQRGVPPGQYVSLCVSDTGTGMSPDVVARAFDPFFTTKPTGQGTGLGLSMVYGFAGQSGG 506 G VS+ +SDTG G++P+ + R F+PFFTTKP G GTGLGLS+ YG + GG Sbjct: 362 ------AGDKVSVSISDTGHGIAPENLNRIFEPFFTTKPVGSGTGLGLSLSYGIVKKHGG 415 Query: 507 AVRIYSELGQGATLCIYLP 525 +R+ S G G T I LP Sbjct: 416 DIRVSSAPGCGTTFTIELP 434 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,225,223 Number of extensions: 101415 Number of successful extensions: 504 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 503 Number of HSP's successfully gapped: 22 Length of query: 677 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 585 Effective length of database: 1,184,250 Effective search space: 692786250 Effective search space used: 692786250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)