BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0079.p (460 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 232 6e-62 gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 225 7e-60 gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 204 9e-54 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 137 3e-33 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 117 2e-27 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 232 bits (591), Expect = 6e-62, Method: Compositional matrix adjust. Identities = 153/467 (32%), Positives = 251/467 (53%), Gaps = 29/467 (6%) Query: 6 SLTVRLTALFALASTAVLVGLGLLIMSTVAKHFTDQDEQLLEKELELIRMVVNERGAGAI 65 SL++RL ALF L + +L LG + ++ +D++ L + I +V A ++ Sbjct: 8 SLSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLVEH--AASL 65 Query: 66 TSTFG------EALHNHPGFFVHISSIDGRPMYSTLADATQPILAAAKRVASGHSF---- 115 + L H V I+ DG STL ++ P R G Sbjct: 66 EDILARRHLVLDTLTGHDQLLVRIARADG----STLLESHDP-----TRTPPGIDLSQVD 116 Query: 116 --AVNAGQHQEFQAIKA-YLKDD---KSSEQLQVIVAVDTEIHDHFMRNFQTTLILYVAG 169 A ++ Q + ++A Y+ +S EQ V+V + +++ + Sbjct: 117 IRAWDSIQSWKLGDLRARYVASKPVTRSGEQAVVLVVRTASDRMLLLAEYRSDVWWAAGV 176 Query: 170 SAVIVVLLSWWAARRGLAPLRAMSDKVQAVSSHNFGERMPIETLPVEIADLAAKLNAMLE 229 A++ +L+S++ R GL PLR M+ + +AVS++ R+ ++ P E+ ++ N ML+ Sbjct: 177 GALLAILMSYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLD 236 Query: 230 RLQQDFHRITNFSTDIAHELRTPITNLLTQTDVVLTQQRTNEAYRDTLSSNAEELQRLAR 289 RL + F +++ FS D+AH++RTP+ NL+ QT V L + R + Y+ LSSN EE +RL+R Sbjct: 237 RLHRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSR 296 Query: 290 TISDMLFLAQTENGISLPSYEPLKLQDEITELLDFYDALAEEKGVNLQLTGDAIVRGDRL 349 + MLFLA+ EN + ++ L + E+T + D+++ +A+E V L + G ++ D Sbjct: 297 MMESMLFLARAENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQADAT 356 Query: 350 MVRRALSNLISNAMRYTPASGSIAVSIARSEDGILVTVENDGPEIPPEHLPHLFDRFYRA 409 ++RRA+ NL++NA+RYTPA G I + + + DG+ ++V N G IP E L LFDRFYRA Sbjct: 357 LLRRAVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRA 416 Query: 410 DKSRTKLDTDSAGLGLSITQAIMRAHQGSVSVRSALGK-TRFTLNFP 455 D SR+ S LGL+I ++IM H G SV S+ G+ T F+L FP Sbjct: 417 DPSRSSAGGSSG-LGLAIVRSIMMLHGGQASVTSSPGQLTVFSLRFP 462 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 225 bits (573), Expect = 7e-60, Method: Compositional matrix adjust. Identities = 144/456 (31%), Positives = 236/456 (51%), Gaps = 21/456 (4%) Query: 4 QRSLTVRLTALFALASTAVLVGLGLLIMSTVAKHFTDQDEQLLEKELELIRMVVNERG-- 61 ++S+T RL + A ++ + G+++ + + + L L I + G Sbjct: 9 RQSITARLVWMSAASALLIFALTGMVLYTVLKGELLRHERDGLVTTLNDISYQIGRAGNT 68 Query: 62 -----AGAITSTFGEALHNHPGFFVHISSIDGRPMYSTLAD-ATQPILAAAKRVASGHSF 115 A +T +A H F+ ISS D R Y L+ A Q I R G+S Sbjct: 69 ERWGRVQAKLATLSQA-DQHVSFW--ISSSDARFTYGDLSRLAPQAI-----RADEGNSV 120 Query: 116 AVN--AGQHQEFQAIKAYLKDDKSSEQLQVIVAVDTEIHDHFMRNFQTTLILYVAGSAVI 173 G+ + L + + V V VD E + R F ++ V+ Sbjct: 121 GRLHLPGRRYPLHIMTRTLPAFEERPAVDVTVGVDGEPYFEMRRTFLLATSVFSLLGIVL 180 Query: 174 VVLLSWWAARRGLAPLRAMSDKVQAVSSHNFGERMPIETLPVEIADLAAKLNAMLERLQQ 233 V+ S+W AR GLAPLR MSD+ Q+ S RMP+E +P E++ LA N L+R++ Sbjct: 181 AVISSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGALDRIED 240 Query: 234 DFHRITNFSTDIAHELRTPITNLLTQTDVVLTQQRTNEAYRDTLSSNAEELQRLARTISD 293 + ++ F+ D+AHELRTP+ NL+ +T V L ++R+ ++D L SN EEL R+ ++D Sbjct: 241 AYTQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRSIVND 300 Query: 294 MLFLAQTENGISLPSYEPLKLQDEITELLDFYDALAEEKGVNLQLTGD--AIVRGDRLMV 351 MLFLA+++ G + + P ++ DE+ + +DF++ + ++ G+ +++ GD A + + Sbjct: 301 MLFLARSDQGEAATARVPARVADEVQKTVDFFEFVLDDSGMRIEVAGDTEATAMMETALF 360 Query: 352 RRALSNLISNAMRYTPASGSIAVSIARSEDGILVTVENDGPEIPPEHLPHLFDRFYRADK 411 +RAL+NLI NA++++P + + IA E+ + V V N G I HLP LFDRFYR D Sbjct: 361 KRALTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRFYRVDS 420 Query: 412 SRTKLD-TDSAGLGLSITQAIMRAHQGSVSVRSALG 446 +R ++ + GLGL+I +A+ R H G S G Sbjct: 421 ARQDINGSHGHGLGLAIVKAVARMHGGRAFASSKEG 456 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 204 bits (520), Expect = 9e-54, Method: Compositional matrix adjust. Identities = 143/465 (30%), Positives = 236/465 (50%), Gaps = 23/465 (4%) Query: 6 SLTVRLTALFALASTAVLVGLGLLIMSTVAKHFTDQDEQLLEKELELIRMVVNERGAGAI 65 +LT R+TALFAL ++ V+ GLG + + + + + +Q L +E R ++++ + Sbjct: 12 TLTTRVTALFALMTSLVVTGLGFYLDRSAGQALSQRADQALIGRVEHFRNLMHD-----L 66 Query: 66 TSTFGEALHNHPGFF---------VHISSIDGR-PMYSTLADATQPI----LAAAKRVAS 111 + E + P F V I G P + D P L V+S Sbjct: 67 YNV--EQMEQRPALFESMMGNEQDVRIFRRQGEAPFIQSNPDHLVPPAMTPLPVGSPVSS 124 Query: 112 GHSFAVNAGQHQEFQAIKAYLKDDKSSEQLQVIVAVDTEIHDHFMRNFQTTLILYVAGSA 171 + + A K + ++++ A MR+++ + A + Sbjct: 125 ASLRTSQRADGVRVRWVSALADVGKGGDTVEIVAAYVMVQEARMMRSYRWRIAGAAAAAV 184 Query: 172 VIVVLLSWWAARRGLAPLRAMSDKVQAVSSHNFGERMPIETLPVEIADLAAKLNAMLERL 231 V+ LL + RRGL PL AMS + ++ + R+ E +P E+ +A NAML+RL Sbjct: 185 VLTALLGFLLLRRGLQPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDRL 244 Query: 232 QQDFHRITNFSTDIAHELRTPITNLLTQTDVVLTQQRTNEAYRDTLSSNAEELQRLARTI 291 + + + FS D+AHE+RTPI L+ Q+ V L Q+R+ E Y L SN EELQRL+R + Sbjct: 245 EAGYAHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRLSRIV 304 Query: 292 SDMLFLAQTENGISLPSYEPLKLQDEITELLDFYDALAEEKGVNLQLTGDAIVRGDRLMV 351 ++LFLAQ ++ L L +E+ + D+++ +A+E+G+ + + + +M Sbjct: 305 ENILFLAQADHAALAVERNALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQANLIMW 364 Query: 352 RRALSNLISNAMRYTPASGSIAVSIARSEDGILVTVENDGPEIPPEHLPHLFDRFYRADK 411 RR +SNL+ NA+RY A +I + +G + VEN ++ + + +FDRFYR D+ Sbjct: 365 RRVVSNLVINAVRYGRAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFYRGDR 424 Query: 412 SRTKLDTDSAGLGLSITQAIMRAHQGSVSVRS-ALGKTRFTLNFP 455 SR+ T+S GLGL+I +AIM H G+ +V A G RF+L FP Sbjct: 425 SRSAF-TESNGLGLAIVKAIMALHGGTAAVECPAPGWIRFSLRFP 468 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 137 bits (344), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 90/290 (31%), Positives = 151/290 (52%), Gaps = 13/290 (4%) Query: 170 SAVIVVLLSWWAARRGLAPLRAMSDKVQAVSSHNFGERMPIETLPVEIADLAAKLNAMLE 229 +++ VVL+ A RRGL PL+++ ++ G + PVE+ L +N ++ Sbjct: 173 ASIFVVLV---AVRRGLRPLKSLQRNLEQRDHGKLGA-IDGRGAPVELQPLIVAMNELMA 228 Query: 230 RLQQDFHRITNFSTDIAHELRTPITNLLTQTDVVLTQQRTNEAYRDTLSSNAEELQRLAR 289 R+ + NF D+AH+LRTP+T L Q +++ + + +L+ ++R+ R Sbjct: 229 RINKGEQAQQNFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIR 288 Query: 290 TISDMLFLAQTENGI-SLPSYEPLKLQDEITELLDFYDALAEEKGVNLQLT-GDAIVRGD 347 +L LA++E G+ E L L + E + + A++K ++L A +RGD Sbjct: 289 QSRQLLALARSEPGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGFDLRPARLRGD 348 Query: 348 RLMVRRALSNLISNAMRYTPASGSIAVSIARSEDGILVTVENDGPEIPPEHLPHLFDRFY 407 R ++ + NLI NA+RY+P G++ V +D +++VE+ GP I PEH +FDRFY Sbjct: 349 RFLLSDLIDNLIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDRFY 408 Query: 408 RADKSRTKLDTDSAGLGLSITQAIMRAHQGSVSV--RSALGKTRFTLNFP 455 RA+ +GLGL+I + I H G +SV + T FT+ FP Sbjct: 409 RANDK-----VAGSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRFP 453 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 117 bits (294), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 96/291 (32%), Positives = 147/291 (50%), Gaps = 16/291 (5%) Query: 176 LLSWWAARRGLAPLRAMSDKVQAVSSHNFGERMPIETLPVEIADLAAKLNAMLERLQQDF 235 +LSW A RGLAP+R + +V A N + + +P EI L LN +L RL+ Sbjct: 155 VLSWVAVSRGLAPVRKAAAEV-AARDMNSLQPLADVGMPQEIQPLTHALNDLLARLKHAT 213 Query: 236 HRITNFSTDIAHELRTPITNLLTQTDVVLTQQRTNEAYRDTLSSNAEE-LQRLARTISDM 294 F D AHELRTP+T L QT L ++ +E R ++ + L+R + + Sbjct: 214 ETQRAFVADAAHELRTPLTALRLQTQ--LAERAVDEEERRAAFADLKNGLERTTHLVHQL 271 Query: 295 LFLAQTENGISLPSYEPLKLQDEITELLDFYDALAEEKGVNLQLT--GD----AIVRGDR 348 L LA+ E S+ + L+ + ++ ++AE G++L L+ GD V G+ Sbjct: 272 LTLARQEPDAM--SFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGNA 329 Query: 349 LMVRRALSNLISNAMRYTPASGSIAVSIARSEDGIL-VTVENDGPEIPPEHLPHLFDRFY 407 +R +NL+ NA+RYTPA G + V + S G V V++ GP I LP +FDRFY Sbjct: 330 DALRILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDRFY 389 Query: 408 RADKSRTKLDTDSA---GLGLSITQAIMRAHQGSVSVRSALGKTRFTLNFP 455 RA S+ + +A GLGL+I + I H+ V +R+ + + FP Sbjct: 390 RAQHSQEGSGSRAAFGSGLGLAIVRQIADLHRVPVELRNTGHGLQARVVFP 440 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,263,778 Number of extensions: 50231 Number of successful extensions: 337 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 272 Number of HSP's successfully gapped: 39 Length of query: 460 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 372 Effective length of database: 1,203,190 Effective search space: 447586680 Effective search space used: 447586680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)