BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0066.p (465 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 564 e-162 gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 265 7e-72 gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 224 1e-59 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 135 1e-32 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 115 1e-26 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 564 bits (1454), Expect = e-162, Method: Compositional matrix adjust. Identities = 292/454 (64%), Positives = 362/454 (79%), Gaps = 2/454 (0%) Query: 1 MTTRLVALFALVAIVTFAAVGTYLYHSLYVQLERRDDEELIGKIRSIDHLIQEAQSIDSI 60 ++ RLVALF L+A+V AA+GTYL HSL+VQLERRDDEEL GK RSI+HL++ A S++ I Sbjct: 9 LSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLVEHAASLEDI 68 Query: 61 KANNHAMLDTISGHDQLLMLITDASGVAILKNYSGPVELRTSL-FSTLQATQTNALQSID 119 A H +LDT++GHDQLL+ I A G +L+++ P + S + +++QS Sbjct: 69 LARRHLVLDTLTGHDQLLVRIARADGSTLLESHD-PTRTPPGIDLSQVDIRAWDSIQSWK 127 Query: 120 WEGNRIRFIASSPTTSAGEKTRVIVARTVSDRMVLLADYRTEVWYAATIGTLLATLLSLF 179 R R++AS P T +GE+ V+V RT SDRM+LLA+YR++VW+AA +G LLA L+S F Sbjct: 128 LGDLRARYVASKPVTRSGEQAVVLVVRTASDRMLLLAEYRSDVWWAAGVGALLAILMSYF 187 Query: 180 LVRSGLRPLRLMAAQTHAVSASRLDTRLDIRSAPKELHEIVESFNAMLDRLHRSFEQLTQ 239 LVRSGLRPLR MAAQT AVSA+RLDTRLD+++AP+ELHEIVESFN MLDRLHRSF+QL+Q Sbjct: 188 LVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRLHRSFQQLSQ 247 Query: 240 FSADLAHDLRTPLNNLMVQTQVALGKPRSLEEYQGLLSSNIEEYERLARMMESMLFLARA 299 FSADLAHD+RTPLNNLMVQTQVALGKPR ++EYQ LLSSNIEEYERL+RMMESMLFLARA Sbjct: 248 FSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMMESMLFLARA 307 Query: 300 ENAHVAVKPQMLSVEDELAKIADYFEGIAEEADVRIVVSGTGDIFADPMLLRRALGNLIS 359 ENAHV ++ Q L V EL +IADYFEGIA+EA VR+VV G G + AD LLRRA+GNL++ Sbjct: 308 ENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQADATLLRRAVGNLVA 367 Query: 360 NAIRYTPKERIIRVEATDLDDATILTVSNPGEGIPPDVVGRIFDRFYRADPSRSSASGSS 419 NAIRYTP + +IR++ D ++VSNPG GIP + + R+FDRFYRADPSRSSA GSS Sbjct: 368 NAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRADPSRSSAGGSS 427 Query: 420 GLGLAIVRSIMMLHGGEATVKSTQGERTIFSLTF 453 GLGLAIVRSIMMLHGG+A+V S+ G+ T+FSL F Sbjct: 428 GLGLAIVRSIMMLHGGQASVTSSPGQLTVFSLRF 461 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 265 bits (676), Expect = 7e-72, Method: Compositional matrix adjust. Identities = 165/458 (36%), Positives = 261/458 (56%), Gaps = 8/458 (1%) Query: 1 MTTRLVALFALVAIVTFAAVGTYLYHSLYVQLERRDDEELIGKIRSIDHLIQEAQSIDSI 60 +TTR+ ALFAL+ + +G YL S L +R D+ LIG++ +L+ + +++ + Sbjct: 13 LTTRVTALFALMTSLVVTGLGFYLDRSAGQALSQRADQALIGRVEHFRNLMHDLYNVEQM 72 Query: 61 KANNHAMLDTISGHDQLLMLITDASGVAILKNYSG----PVELRTSLFSTLQATQTNALQ 116 + A+ +++ G++Q + + +++ P + S + + Q Sbjct: 73 E-QRPALFESMMGNEQDVRIFRRQGEAPFIQSNPDHLVPPAMTPLPVGSPVSSASLRTSQ 131 Query: 117 SIDWEGNRIRFIAS-SPTTSAGEKTRVIVARTVSDRMVLLADYRTEVWYAATIGTLLATL 175 D G R+R++++ + G+ ++ A + ++ YR + AA +L L Sbjct: 132 RAD--GVRVRWVSALADVGKGGDTVEIVAAYVMVQEARMMRSYRWRIAGAAAAAVVLTAL 189 Query: 176 LSLFLVRSGLRPLRLMAAQTHAVSASRLDTRLDIRSAPKELHEIVESFNAMLDRLHRSFE 235 L L+R GL+PL M+ + ++ L RLD P EL + SFNAMLDRL + Sbjct: 190 LGFLLLRRGLQPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDRLEAGYA 249 Query: 236 QLTQFSADLAHDLRTPLNNLMVQTQVALGKPRSLEEYQGLLSSNIEEYERLARMMESMLF 295 L QFS DLAH++RTP+N LM Q+QVALG+ RS EEY+ +L SN+EE +RL+R++E++LF Sbjct: 250 HLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRLSRIVENILF 309 Query: 296 LARAENAHVAVKPQMLSVEDELAKIADYFEGIAEEADVRIVVSGTGDIFADPMLLRRALG 355 LA+A++A +AV+ L + +EL +I DYFEGIA+E +R V G A+ ++ RR + Sbjct: 310 LAQADHAALAVERNALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQANLIMWRRVVS 369 Query: 356 NLISNAIRYTPKERIIRVEATDLDDATILTVSNPGEGIPPDVVGRIFDRFYRADPSRSSA 415 NL+ NA+RY E IR+ A + + + V N E + + R+FDRFYR D SRS+ Sbjct: 370 NLVINAVRYGRAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFYRGDRSRSAF 429 Query: 416 SGSSGLGLAIVRSIMMLHGGEATVKSTQGERTIFSLTF 453 + S+GLGLAIV++IM LHGG A V+ FSL F Sbjct: 430 TESNGLGLAIVKAIMALHGGTAAVECPAPGWIRFSLRF 467 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 224 bits (570), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 155/461 (33%), Positives = 249/461 (54%), Gaps = 25/461 (5%) Query: 1 MTTRLVALFALVAIVTFAAVGTYLYHSLYVQLERRDDEELIGKIRSIDHLIQEAQSIDSI 60 +T RLV + A A++ FA G LY L +L R + + L+ + I + I A + + Sbjct: 12 ITARLVWMSAASALLIFALTGMVLYTVLKGELLRHERDGLVTTLNDISYQIGRAGNTERW 71 Query: 61 KANNHAMLDTISGHDQLLMLITDASGVAILKNYSGPVELRTSLFSTLQATQTNALQSIDW 120 A L T+S DQ + +S +L ++A + N++ + Sbjct: 72 -GRVQAKLATLSQADQHVSFWISSSDARFTYG-----DLSRLAPQAIRADEGNSVGRLHL 125 Query: 121 EGNRIRFIASSPTTSAGEKTRVIVARTVS---------DRMVLLADYRTEVWYAATIGTL 171 G R + T A E+ R V TV R LLA T V+ + +G + Sbjct: 126 PGRRYPLHIMTRTLPAFEE-RPAVDVTVGVDGEPYFEMRRTFLLA---TSVF--SLLGIV 179 Query: 172 LATLLSLFLVRSGLRPLRLMAAQTHAVSASRLDTRLDIRSAPKELHEIVESFNAMLDRLH 231 LA + S ++ R+GL PLR M+ + + S +L R+ + + P+EL + +FN LDR+ Sbjct: 180 LAVISSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGALDRIE 239 Query: 232 RSFEQLTQFSADLAHDLRTPLNNLMVQTQVALGKPRSLEEYQGLLSSNIEEYERLARMME 291 ++ QL F+AD+AH+LRTPL NL+ +TQVAL + RS E++ +L SN+EE +R+ ++ Sbjct: 240 DAYTQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRSIVN 299 Query: 292 SMLFLARAENAHVAVKPQMLSVEDELAKIADYFEGIAEEADVRIVVSGTGDIFA--DPML 349 MLFLAR++ A V DE+ K D+FE + +++ +RI V+G + A + L Sbjct: 300 DMLFLARSDQGEAATARVPARVADEVQKTVDFFEFVLDDSGMRIEVAGDTEATAMMETAL 359 Query: 350 LRRALGNLISNAIRYTPKERIIRVEATDLDDATILTVSNPGEGIPPDVVGRIFDRFYRAD 409 +RAL NLI NAI+++P+ ++R++ ++ + VSNPG+ I + R+FDRFYR D Sbjct: 360 FKRALTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRFYRVD 419 Query: 410 PSRSSASGS--SGLGLAIVRSIMMLHGGEATVKSTQGERTI 448 +R +GS GLGLAIV+++ +HGG A S +G +I Sbjct: 420 SARQDINGSHGHGLGLAIVKAVARMHGGRAFASSKEGVNSI 460 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 135 bits (339), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 98/290 (33%), Positives = 147/290 (50%), Gaps = 14/290 (4%) Query: 170 TLLATLLSLFLVRSGLRPLRLMAAQTHAVSASRLDTRLDIRSAPKELHEIVESFNAMLDR 229 T + + L VR GLRPL+ + +L +D R AP EL ++ + N ++ R Sbjct: 171 TFASIFVVLVAVRRGLRPLKSLQRNLEQRDHGKLGA-IDGRGAPVELQPLIVAMNELMAR 229 Query: 230 LHRSFEQLTQFSADLAHDLRTPLNNLMVQTQVALGKPRSLEEYQGLLSSNIEEYERLARM 289 +++ + F AD+AH LRTPL L +Q ++ K + + L+ ER+ R Sbjct: 230 INKGEQAQQNFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQ 289 Query: 290 MESMLFLARAENA-HVAVKPQMLSVEDELAKIADYFEGIAEEADVRIVVSG----TGDIF 344 +L LAR+E + K + LS++ + + +F EEAD + + G + Sbjct: 290 SRQLLALARSEPGLFESRKLEELSLDKLVEESVQHF---IEEADKKRIDLGFDLRPARLR 346 Query: 345 ADPMLLRRALGNLISNAIRYTPKERIIRVEATDLDDATILTVSNPGEGIPPDVVGRIFDR 404 D LL + NLI NA+RY+P + V + DAT+L+V + G GI P+ IFDR Sbjct: 347 GDRFLLSDLIDNLIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDR 406 Query: 405 FYRADPSRSSASGSSGLGLAIVRSIMMLHGGEATVKST-QGERTIFSLTF 453 FYRA+ SGLGLAIVR I HGG +V QG+ TIF++ F Sbjct: 407 FYRAN----DKVAGSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRF 452 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 115 bits (287), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 114/373 (30%), Positives = 173/373 (46%), Gaps = 35/373 (9%) Query: 75 DQLLMLITDASGVAILKNYSGPVELRTSLFSTLQATQTNALQSIDWEGNRIRFIASSPTT 134 D+++M I D+ G I +++ R + + N + W + S+ Sbjct: 69 DEIMMQIWDSRGAVIYHSHARGAMPRQAELGFANVSGPNGM----WR------VYSAQIG 118 Query: 135 SAGEKTRVIVARTVSDRMVLLADYRTEVWYAATIGTLLATLLSLFLVRSGLRPLRLMAAQ 194 S T V VA+ S R + A + + L +LS V GL P+R AA+ Sbjct: 119 S----TVVQVAQPQSARDEIAAQMAVKTVVPLLLLFPLIGVLSWVAVSRGLAPVRKAAAE 174 Query: 195 THAVSASRLDTRLDIRSAPKELHEIVESFNAMLDRLHRSFEQLTQFSADLAHDLRTPLNN 254 A + L D+ P+E+ + + N +L RL + E F AD AH+LRTPL Sbjct: 175 VAARDMNSLQPLADV-GMPQEIQPLTHALNDLLARLKHATETQRAFVADAAHELRTPLTA 233 Query: 255 LMVQTQVALGKPRSLEEYQGLLSSNIEEYERLARMMESMLFLARAEN-----AHVAVKPQ 309 L +QTQ+A + EE + + ER ++ +L LAR E V ++ Sbjct: 234 LRLQTQLA-ERAVDEEERRAAFADLKNGLERTTHLVHQLLTLARQEPDAMSFTQVDLRAL 292 Query: 310 MLSVEDELAKIADYFE---GIAEEADVRIVVSGTGDIFADPMLLRRALGNLISNAIRYTP 366 + SV +L+ +A++ G++++ D + TG+ A LR NL+ NA+RYTP Sbjct: 293 LGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGNADA----LRILFNNLVDNALRYTP 348 Query: 367 KERIIRVEATDLDDATILT--VSNPGEGIPPDVVGRIFDRFYRADPSR----SSASGSSG 420 ++ V DL A T V + G GI + R+FDRFYRA S+ S A+ SG Sbjct: 349 AGGVVDV-GVDLSTAGYCTVVVQDSGPGIAESELPRVFDRFYRAQHSQEGSGSRAAFGSG 407 Query: 421 LGLAIVRSIMMLH 433 LGLAIVR I LH Sbjct: 408 LGLAIVRQIADLH 420 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,195,093 Number of extensions: 43590 Number of successful extensions: 283 Number of sequences better than 1.0e-08: 41 Number of HSP's gapped: 235 Number of HSP's successfully gapped: 41 Length of query: 465 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 376 Effective length of database: 1,198,455 Effective search space: 450619080 Effective search space used: 450619080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)