BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0065.p (223 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309361|ref|YP_003773453.1| response regulator for cobalt/z... 412 e-116 gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance ... 291 3e-80 gi|300311799|ref|YP_003775891.1| two component heavy metal respo... 266 1e-72 gi|300313680|ref|YP_003777772.1| two component response regulato... 164 5e-42 gi|300312680|ref|YP_003776772.1| two component response regulato... 157 8e-40 >gi|300309361|ref|YP_003773453.1| response regulator for cobalt/zinc/cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 412 bits (1058), Expect = e-116, Method: Compositional matrix adjust. Identities = 205/219 (93%), Positives = 212/219 (96%) Query: 1 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQSGVDGLHNARTLEYKLIILDVMLPGMNGW 60 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQ+GVDGLHNARTL+YKLIILDVMLPGMNGW Sbjct: 1 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGW 60 Query: 61 DILRELRRTHDTPVLFLTARDEIEDRVKGLELGADDYLVKPFAFTELLARVRTLLRRGPS 120 DILRELRRTHDTPVLFLTARDE+EDRVKGLELGADDYLVKPFA+TELLARVRTLLRRGPS Sbjct: 61 DILRELRRTHDTPVLFLTARDEVEDRVKGLELGADDYLVKPFAYTELLARVRTLLRRGPS 120 Query: 121 RESDVIEYAGVQLEVLRRKVTRDGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWDM 180 RE D+IEY V L+VLRRKVTR GQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWDM Sbjct: 121 REVDLIEYDDVVLDVLRRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWDM 180 Query: 181 NFDSDTNVVDVAVRRLRAKLDDGFEGKLLHTAWGMGYVF 219 NFDSDTNVVDVAVRRLRAKLD+GFE KLLHT WGMGYVF Sbjct: 181 NFDSDTNVVDVAVRRLRAKLDEGFEKKLLHTVWGMGYVF 219 >gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 291 bits (745), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 138/220 (62%), Positives = 177/220 (80%) Query: 1 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQSGVDGLHNARTLEYKLIILDVMLPGMNGW 60 M IL++ED+PK+GDYLR+GL ESG+ VD ++G DGLH A +Y L++LDVMLPG++GW Sbjct: 1 MAILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGW 60 Query: 61 DILRELRRTHDTPVLFLTARDEIEDRVKGLELGADDYLVKPFAFTELLARVRTLLRRGPS 120 ++ LR D PVLFLTARD ++DR++GL+LGADDYLVKPF+FTEL+ R+RTLLRRG + Sbjct: 61 QVMSALRSKRDLPVLFLTARDHVDDRIRGLQLGADDYLVKPFSFTELVLRIRTLLRRGVT 120 Query: 121 RESDVIEYAGVQLEVLRRKVTRDGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWDM 180 RE++V E A +QL+ +R KVTR G I LT KEF LLH+LI+R GE LSR IASQ+WDM Sbjct: 121 REAEVYEIADLQLDHVRHKVTRQGVNIALTNKEFMLLHLLIKRQGEALSRSVIASQIWDM 180 Query: 181 NFDSDTNVVDVAVRRLRAKLDDGFEGKLLHTAWGMGYVFE 220 NFDSDTNVVDVA++RLRAK+D F+ KL+HT +GY+F Sbjct: 181 NFDSDTNVVDVAIKRLRAKIDAPFDKKLIHTVRSVGYMFS 220 >gi|300311799|ref|YP_003775891.1| two component heavy metal response transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 266 bits (679), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 131/222 (59%), Positives = 167/222 (75%) Query: 1 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQSGVDGLHNARTLEYKLIILDVMLPGMNGW 60 M IL+VEDE K +YL +GLTE G VD +GVDG H A +Y +I+LDVMLPG++G+ Sbjct: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAIEHDYDVIVLDVMLPGLDGF 60 Query: 61 DILRELRRTHDTPVLFLTARDEIEDRVKGLELGADDYLVKPFAFTELLARVRTLLRRGPS 120 +LR LR+ +TPV+ LTARD +EDR+KGL GADDYL+KPF+F ELLAR++ L RRG + Sbjct: 61 SVLRALRKVKETPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGRA 120 Query: 121 RESDVIEYAGVQLEVLRRKVTRDGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWDM 180 +E + +Q+++L RK R G RIDLTAKEFALL ++ RR GE+LS+ IA VWDM Sbjct: 121 QEPAQLRIGDLQIDLLSRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWDM 180 Query: 181 NFDSDTNVVDVAVRRLRAKLDDGFEGKLLHTAWGMGYVFEAR 222 NFDS+TNVV+VA++RLRAK+D FE KLLHT GMGYV E R Sbjct: 181 NFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVLEPR 222 >gi|300313680|ref|YP_003777772.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 218 Score = 164 bits (415), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 91/222 (40%), Positives = 133/222 (59%), Gaps = 5/222 (2%) Query: 1 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQSGVDGLHNARTLEYKLIILDVMLPGMNGW 60 M +LLVED+P G+ +R+GL + GF +DWVQ G RT +Y +++LD+ LP +G Sbjct: 1 MRVLLVEDDPMVGEAVRKGLRQDGFAIDWVQDGKSADVALRTEDYAMLLLDLGLPQKDGL 60 Query: 61 DILRELR-RTHDTPVLFLTARDEIEDRVKGLELGADDYLVKPFAFTELLARVRTLLRRGP 119 +LR LR R + PVL TARD + DRV GL+ GADDYL+KPF EL AR+R L RR Sbjct: 61 AVLRTLRERGNAIPVLITTARDAVADRVAGLDAGADDYLIKPFDLEELSARMRALSRRQA 120 Query: 120 SRESDVIEYAGVQLEVLRRKVTRDGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWD 179 R +++ V L +VT G+ ++L+A+EF LL + RPG VLSR Q+ +++ Sbjct: 121 GRAESLVQVREVILNPATHEVTVSGKPVNLSAREFTLLQAFMDRPGVVLSRAQLEEKLYG 180 Query: 180 MNFDSDTNVVDVAVRRLRAKLDDGFEGKLLHTAWGMGYVFEA 221 + ++N V+V + LR K+ F + G+GY+ A Sbjct: 181 WDDSIESNAVEVHIHALRKKVGSDF----IKNVRGVGYLVPA 218 >gi|300312680|ref|YP_003776772.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 220 Score = 157 bits (396), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 91/221 (41%), Positives = 136/221 (61%), Gaps = 6/221 (2%) Query: 1 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQSGVDGLHNARTLEYKLIILDVMLPGMNGW 60 M++LLVED+P D L R L GF V V +G D L ++ +++LD+ LPG++G+ Sbjct: 1 MNVLLVEDDPVLTDGLSRVLNSHGFTVHTVNNGNDAL--SQRFNAAVLVLDIGLPGIDGF 58 Query: 61 DILRELRRT-HDTPVLFLTARDEIEDRVKGLELGADDYLVKPFAFTELLARVRTLLRRGP 119 ++LR +R ++TPVL LTARD I DRV GLELGADDYLVKPFA EL+AR++ L+RR Sbjct: 59 EVLRRMRAAGNNTPVLLLTARDTIPDRVHGLELGADDYLVKPFATPELVARIKALIRRSA 118 Query: 120 SRESDVIEYAGVQLEVLRRKVTRDGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWD 179 + + + G+ L+ ++ DG++I+L+ +E+ +L L++ V+S+ QI V Sbjct: 119 PQPAQ-LTVGGLSLDNATKRADIDGRKIELSLREWTVLEYLMQHASRVVSKQQIIDAVLP 177 Query: 180 MNFDSDTNVVDVAVRRLRAKLDDGFEGKLLHTAWGMGYVFE 220 D N V+V V R+R K+ D G + T G GY+ E Sbjct: 178 WGEDFTINAVEVYVSRIRLKIAD--SGVAIRTIRGFGYMLE 216 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.141 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 719,595 Number of extensions: 29591 Number of successful extensions: 215 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 198 Number of HSP's successfully gapped: 39 Length of query: 223 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 141 Effective length of database: 1,231,600 Effective search space: 173655600 Effective search space used: 173655600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)