BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0051.p (401 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 781 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 237 1e-63 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 203 2e-53 gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 147 1e-36 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 99 6e-22 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 781 bits (2016), Expect = 0.0, Method: Compositional matrix adjust. Identities = 385/401 (96%), Positives = 390/401 (97%), Gaps = 7/401 (1%) Query: 1 MIHLPGLNFDHGEDIAALREAVAAFAQTEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGIT 60 MIHLPGL+FDHGEDIAALREAVAAFA +EIAPRAAEIDRSDQFPMDLWKKLGDLGVLGIT Sbjct: 1 MIHLPGLSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGIT 60 Query: 61 VGEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPKL 120 V EEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLP+L Sbjct: 61 VSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRL 120 Query: 121 ISGDFIGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDL 180 ISGDFIGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDL Sbjct: 121 ISGDFIGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDL 180 Query: 181 DAGARGMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNV 240 +AGARGMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNV Sbjct: 181 EAGARGMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNV 240 Query: 241 LMSGLDFERSVLSGGPLGIMQXCVCVCVCVCVCVVPYVHDRKQFGQAIGEFQLMQGKLAD 300 LMSGLDFERSVLSGGPLGIMQ C+ V VVPYVHDRKQFGQAIGEFQLMQGKLAD Sbjct: 241 LMSGLDFERSVLSGGPLGIMQACMDV-------VVPYVHDRKQFGQAIGEFQLMQGKLAD 293 Query: 301 MYSTMMACKAYVYAVGQACDRADSADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGN 360 MYSTMMACKAYVYAVGQACDRADSADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGN Sbjct: 294 MYSTMMACKAYVYAVGQACDRADSADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGN 353 Query: 361 GYINEYPVGRLWRDAKLYEIGAGTSEIRRMLIGRELFADTL 401 GYINEYPVGRLWRDAKLYEIGAGTSEIRRMLIGRELFADTL Sbjct: 354 GYINEYPVGRLWRDAKLYEIGAGTSEIRRMLIGRELFADTL 394 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 237 bits (604), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 127/382 (33%), Positives = 219/382 (57%), Gaps = 13/382 (3%) Query: 18 LREAVAAFAQTEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVGEEYGGAGLGYLAHII 77 +R+A+ FAQ + P AA+ DR+ FP + K+L +LG +G+ V E++GGAG+ Y++ ++ Sbjct: 10 IRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGMDYMSLVL 69 Query: 78 AMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPKLISGDFIGALAMSEPNAG 137 A+EEI+ + ++L ++ G+ QK ++L L G+ +G ++EP+ G Sbjct: 70 ALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGCFCLTEPHTG 129 Query: 138 SDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLDAGARGMTAFLVEKGYK 197 SD +++ RA++ GD ++LNG+K +IT+G A V +V+A TD AG +G++ FLV Sbjct: 130 SDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCFLVPCDTP 189 Query: 198 GFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPL 257 GF V + +K+G S T +++ +C VP +LG G G + +S L+ G + Sbjct: 190 GFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLE-------AGRI 242 Query: 258 GIMQXCVCVCVCVCVCVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQ 317 GI V + V Y R+ FG I E Q + +LADM + + A + V+ Q Sbjct: 243 GIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRAAQ 302 Query: 318 ACDRADSADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNGYINEYPVGRLWRDAKL 377 D+ R K+A+ A ++++E A +A +AIQ GG GY +++PV R++RD ++ Sbjct: 303 LKDQG------RPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRI 356 Query: 378 YEIGAGTSEIRRMLIGRELFAD 399 +I G ++I+R++IGR + +D Sbjct: 357 CQIYEGANDIQRLVIGRAIASD 378 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 203 bits (516), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 134/392 (34%), Positives = 203/392 (51%), Gaps = 16/392 (4%) Query: 7 LNFDHGEDIAALREAVAAFAQTEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVGEEYG 66 ++F+ +D A +++ FA E+AP AA+ D FP+D+ K G+LG G+ E G Sbjct: 1 MDFELNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADG 60 Query: 67 GAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPKLISGDFI 126 G GL L + EE++RA S H N+ I G K ++ +L +G I Sbjct: 61 GLGLSRLDATVVFEELARACTSTAAYLTIH-NMVSWMIASWGQPSLKAQWCEQLAAGRKI 119 Query: 127 GALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLDAGARG 186 G+ ++EP +GSD S+K A + YVLNGSK +I+ DVLVV A+T + GARG Sbjct: 120 GSYCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGE-GARG 178 Query: 187 MTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLD 246 ++A +V G S +K K+G T + F + +VP +++LG G+G M GLD Sbjct: 179 VSAIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLD 238 Query: 247 FERSVLSGGPLGIMQXCVCVCVCVCVCVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMM 306 GG + I V Y+ +RKQFG+ I +FQ +Q KLADM + ++ Sbjct: 239 -------GGRINIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELV 291 Query: 307 ACKAYVYAVGQACDR-ADSADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNGYINE 365 A + V D + A A+ K A + + + EA+Q GG GYI E Sbjct: 292 AARQMVRLAATKLDAGSPEATTYCAMAKRLATDLGFR------VCNEALQLHGGYGYIRE 345 Query: 366 YPVGRLWRDAKLYEIGAGTSEIRRMLIGRELF 397 YP+ R +RD ++++I GT+EI R++I R+L Sbjct: 346 YPLERYFRDVRVHQILEGTNEIMRVIISRKLL 377 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 147 bits (372), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 118/378 (31%), Positives = 191/378 (50%), Gaps = 22/378 (5%) Query: 16 AALREAVAAFAQTEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVGEEYGGAGLGYLAH 75 A LR+AV +T +ID + +P L G L + +EYGG+GLG Sbjct: 11 ADLRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLGLTEA 70 Query: 76 IIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPKLISGDF-IGALAMSEP 134 + MEEI+R + G +G N+ + R+G+ EQK +YLP++ SG+ + ++A++EP Sbjct: 71 SVIMEEINRTGGNSGACHGQMYNM--GTLLRHGSPEQKERYLPRIASGELRLQSMAVTEP 128 Query: 135 NAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKT----DLDAGARGMTAF 190 G+D +K A KKGDRYV+NG K+WI+ +D++++ A+T + + GM+ F Sbjct: 129 TTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVSRKSEGMSIF 188 Query: 191 LVE-KGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLDFER 249 LV+ K G + + L M T EL F + E+P EN++G GRG ++ GL+ ER Sbjct: 189 LVDLKEAIGHGMEVR-PILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYILDGLNAER 247 Query: 250 SVLSGGPLGIMQXCVCVCVCVCVCVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACK 309 ++++ C+ Y +R F + IG Q +Q LA+ Y + A Sbjct: 248 TLIAA-------ECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEVEAAN 300 Query: 310 AYVYAVGQACDRADSADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNGYINEYPVG 369 Y QAC D+ A A A +A+ A A +Q+ GG G+ EY V Sbjct: 301 LMRY---QACALFDAQQSCGA---QANMAKYLAAKAAWEAANVCLQTHGGFGFACEYDVE 354 Query: 370 RLWRDAKLYEIGAGTSEI 387 R +R+ +LY++ ++ + Sbjct: 355 RKFRETRLYQVAPISTNL 372 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 99.0 bits (245), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 95/408 (23%), Positives = 179/408 (43%), Gaps = 42/408 (10%) Query: 7 LNFDHGEDIAALREAVAAFAQ---TEIAPRAAEIDRSDQ---FPMDLWKKLGDLGVLGIT 60 ++F E AL+ AFA ++ P + + D+ ++ + D G + Sbjct: 3 IDFQLSESQKALQAGARAFAADVLRKVGPTITPLVKPDERFYATRPFYQAMADAGFIKAL 62 Query: 61 VGEEYGGAGLGYLAHIIAMEEISR-----ASASVGLSYGAHSNLCVNQIKRNGNEEQKRK 115 + E +GG L L +A EE++ SA +G G H+ + +G EQK++ Sbjct: 63 LPESFGGTALSTLDFALAAEELTAVDVNVPSALLGTGLGLHA------VVHHGTPEQKQR 116 Query: 116 YLPKLIS-GDFIGALAMSEPNAGSDV------VSMKLRADKKGDRYVLNGSKMWITNGPD 168 +LP I G + ALA +E G++ V ++ A +GD +V+NG+K + TNG Sbjct: 117 FLPDFIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEWVINGAKHYTTNGTG 176 Query: 169 AD-----VLVVYAKTDLD-AGARGMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQD 222 D + V +TD + + +V G + LD +G R + + + F + Sbjct: 177 WDGKGAHLYSVICRTDPALPPQQSLAVIMVPGNAPGIEIVGLLDTMGHRATISPRVHFNN 236 Query: 223 CEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPLGIMQXCVCVCVCVCVCVVPYVHDRK 282 VP N++G G G+++L + + +++ +G+M+ + + DR+ Sbjct: 237 VRVPASNIVGKPGDGISMLEAAFSWTAALIGSACVGVMRTAFDIALRFAQS------DRR 290 Query: 283 QFGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRADSADKVRALRKDAAGAILYS 342 + E Q + LAD+ M +A Y ++C + D + R+ A Y Sbjct: 291 SGTIPVIEHQNVGFMLADI---KMRIEAARYLTWKSCHQLDLTEGRS--RELAIITKTYC 345 Query: 343 AEKATWMAGEAIQSLGGNGYINEYPVGRLWRDAKLYEI-GAGTSEIRR 389 +E + +A++ +G + Y + YP+ L +DA + + G +RR Sbjct: 346 SELCVQVVYDAMRVVGVDAYTDLYPLAGLMQDALCFPLYDGGNMGVRR 393 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,151,269 Number of extensions: 45050 Number of successful extensions: 147 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 129 Number of HSP's successfully gapped: 12 Length of query: 401 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 314 Effective length of database: 1,207,925 Effective search space: 379288450 Effective search space used: 379288450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)