BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0050.p (200 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313970|ref|YP_003778062.1| 2-hydroxychromene-2-carboxylate... 369 e-103 gi|300310604|ref|YP_003774696.1| 2-hydroxychromene-2-carboxylate... 94 7e-21 gi|300309925|ref|YP_003774017.1| 2-hydroxychromene-2-carboxylate... 74 6e-15 >gi|300313970|ref|YP_003778062.1| 2-hydroxychromene-2-carboxylate isomerase [Herbaspirillum seropedicae SmR1] Length = 200 Score = 369 bits (946), Expect = e-103, Method: Compositional matrix adjust. Identities = 177/200 (88%), Positives = 187/200 (93%) Query: 1 MSTPIDFYFDFSSPYGYFAAVEIDKLAAKYSRSVNWHPILLGPIFKALGTNSLANIPVKG 60 MS PIDFYFDFSSPYGYFAAVEIDKLAA++ R VNWHPILLGPIFKALGTNSL NIPVKG Sbjct: 1 MSNPIDFYFDFSSPYGYFAAVEIDKLAAQHGRDVNWHPILLGPIFKALGTNSLVNIPVKG 60 Query: 61 DYSRHDMERTARFHNIFYKAPSQFPIGTQVAARATLWVQQTQPAKAVDLIKTLYSAYFTE 120 +YSRHDMERTARFHNIFYKAPS FPIGTQVAARATLWVQQTQPAKAV+LIKTLYSAYFTE Sbjct: 61 EYSRHDMERTARFHNIFYKAPSHFPIGTQVAARATLWVQQTQPAKAVELIKTLYSAYFTE 120 Query: 121 DIDISVPENVLRIAADNGIDRAALETALGTPELKEQLRAATEAAGKAGVFGSPFMIVDGE 180 DIDISV +NVLRIAAD GIDR AL+ AL +PELK+QLR +TEAAG+AGVFGSPFMIVDGE Sbjct: 121 DIDISVVDNVLRIAADLGIDRNALQAALESPELKDQLRQSTEAAGQAGVFGSPFMIVDGE 180 Query: 181 QFWGFDRFTQLEAFLKNGKI 200 QFWGFDRF QLEA LKNGKI Sbjct: 181 QFWGFDRFPQLEALLKNGKI 200 >gi|300310604|ref|YP_003774696.1| 2-hydroxychromene-2-carboxylate isomerase [Herbaspirillum seropedicae SmR1] Length = 206 Score = 94.0 bits (232), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 57/186 (30%), Positives = 87/186 (46%), Gaps = 4/186 (2%) Query: 5 IDFYFDFSSPYGYFAAVEIDKLAAKYSRSVNWHPILLGPIFKALG--TNSLANIPVKGDY 62 I+++FDF S Y Y + + I++ A + V W P LLGP+F+ LG T+ KG Y Sbjct: 8 IEYWFDFGSNYSYLSTMRIEQAAQQAGVRVVWRPFLLGPVFQELGWSTSPFVLQKEKGAY 67 Query: 63 SRHDMERTARFHNIFYKAPSQFPIGTQVAARATLWVQQTQPAKAVDLIKTLYSAYFTEDI 122 + DM R R + + + PS+FP +A R L + + + + F ED Sbjct: 68 TWKDMTRQCRKYGLPWTMPSRFPRAAVLATRVALSFEDALWMSS--FCRLVMHLNFAEDR 125 Query: 123 DISVPENVLRIAADNGIDRAALETALGTPELKEQLRAATEAAGKAGVFGSPFMIVDGEQF 182 DI E + + G+ A + + + +LR T A G+FG+P V E F Sbjct: 126 DIDSVETISEVLQALGLPFAEIIAEAQSEPNRARLREQTRQARTLGIFGAPTFFVGDEMF 185 Query: 183 WGFDRF 188 WG DR Sbjct: 186 WGNDRL 191 >gi|300309925|ref|YP_003774017.1| 2-hydroxychromene-2-carboxylate isomerase [Herbaspirillum seropedicae SmR1] Length = 201 Score = 74.3 bits (181), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 61/200 (30%), Positives = 91/200 (45%), Gaps = 4/200 (2%) Query: 1 MSTPIDFYFDFSSPYGYFAAVEIDKLAAKYSRSVNWHPILLGPIFKALGTNSLAN-IPVK 59 MS +++F SP+ Y LA KY + P L IF G LA P + Sbjct: 1 MSKVCEYFFAPHSPFAYLGHDRFVALADKYQVQILPKPFDLSKIFGVSGGLPLAKRAPQR 60 Query: 60 GDYSRHDMERTARF-HNIFYKAPSQFPIGTQVAARATLWVQQTQ-PAKAVDLIKTLYSAY 117 Y ++ R + F P+ FP+ + AAR + Q A A+ L + A Sbjct: 61 QAYRLKELARWSEFLGKPMILQPAHFPVNSDNAARLIIATQLAHGTAAALRLAGAVMRAV 120 Query: 118 FTEDIDISVPENVLRIAADNGIDRAALETALGTPELKEQLRAATEAAGKAGVFGSPFMIV 177 + E +I+ P+ ++ IA D AAL + T ++ + T+ A A VFG+P+ I Sbjct: 121 WEEQKNIADPDTLVAIANVQEHDGAALLKSAETASVQAEFDRFTDEANAASVFGAPWFIY 180 Query: 178 DGEQFWGFDRFTQLE-AFLK 196 +GE +WG DR LE AF K Sbjct: 181 EGEGYWGQDRLDFLERAFAK 200 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 621,838 Number of extensions: 24369 Number of successful extensions: 90 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 87 Number of HSP's successfully gapped: 3 Length of query: 200 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 119 Effective length of database: 1,236,335 Effective search space: 147123865 Effective search space used: 147123865 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)