BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0036.0041.p
         (328 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313977|ref|YP_003778069.1| 2-nitropropane dioxygenase [Her...   606   e-175
gi|300312741|ref|YP_003776833.1| 2-nitropropane dioxygenase [Her...    70   2e-13

>gi|300313977|ref|YP_003778069.1| 2-nitropropane dioxygenase
           [Herbaspirillum seropedicae SmR1]
          Length = 324

 Score =  606 bits (1563), Expect = e-175,   Method: Compositional matrix adjust.
 Identities = 305/320 (95%), Positives = 312/320 (97%)

Query: 1   MPPAPAYPKALQDLRLPIICSPMFIASGPALVAAQCKAGLVGSFPALNARPAELLDTWLT 60
           M  APAYPKALQDLRLPIICSPMFIASGPALVAAQCKAGLVGSFPALNARPAELLDTWLT
Sbjct: 1   MTSAPAYPKALQDLRLPIICSPMFIASGPALVAAQCKAGLVGSFPALNARPAELLDTWLT 60

Query: 61  QLQDELTQWQAEHPQRKAGPIAVNQIVHQSNDRLAQDVATCVKHQVPIIISSLRAPPKEM 120
           QLQ+EL QWQAEH QRK GPIAVNQIVHQSNDRLAQDVATCVKHQVPIIISSLRAPP+EM
Sbjct: 61  QLQEELAQWQAEHAQRKVGPIAVNQIVHQSNDRLAQDVATCVKHQVPIIISSLRAPPQEM 120

Query: 121 LDAVHSYGGIVLHDVISLRHAEKALEAGVDGLILVAAGAGGHAGTLSPFALVGEVRKMFK 180
           LDAVHSYGGIVLHDVISLRHAEKALEAGVDGLILVAAGAGGHAGTLSPFALVGEVRKMFK
Sbjct: 121 LDAVHSYGGIVLHDVISLRHAEKALEAGVDGLILVAAGAGGHAGTLSPFALVGEVRKMFK 180

Query: 181 GPLALSGSIATGDAVLAAQAMGADFAYMGSRWLATRESNVDDAYRQAIVESTAADVVYTN 240
           GPL LSG+IATGDAVLAAQAMGADFAYMGSRWLAT+ESNVD AYRQAIV+STAADVVYTN
Sbjct: 181 GPLVLSGAIATGDAVLAAQAMGADFAYMGSRWLATQESNVDQAYRQAIVDSTAADVVYTN 240

Query: 241 LFTGVHGNYLKKSIVNAGLDPDNLPEADKTKMNFGSGGGSKAKAWRDIWGAGQGVGLMED 300
           LFTGVHGNYLKKSIVNAGLDPDNLPEADKTKMNFGSGGGSKAKAWRDIWGAGQGVGLM+D
Sbjct: 241 LFTGVHGNYLKKSIVNAGLDPDNLPEADKTKMNFGSGGGSKAKAWRDIWGAGQGVGLMDD 300

Query: 301 VPTVAEMVDRLEEEYRAARA 320
           VPTVAEMVDRLE EYRAAR+
Sbjct: 301 VPTVAEMVDRLEAEYRAARS 320


>gi|300312741|ref|YP_003776833.1| 2-nitropropane dioxygenase
           [Herbaspirillum seropedicae SmR1]
          Length = 356

 Score = 70.5 bits (171), Expect = 2e-13,   Method: Compositional matrix adjust.
 Identities = 77/281 (27%), Positives = 129/281 (45%), Gaps = 30/281 (10%)

Query: 58  WLTQLQDELTQWQAEHPQRKAGPIAVNQIVHQSNDRLAQDVATCVKHQVPIIISSLRAPP 117
           WL  LQ  L ++      R A P+   + ++QS +   Q     ++H   ++      PP
Sbjct: 88  WLAHLQPLLDEFGG----RIAAPL---KEIYQSFNADTQMQQVVLEHAPAVVSFHFGLPP 140

Query: 118 KEMLDAVHSYGGIVLHDVISLRHAEKALEAGVDGLILVAAGAGGHAGTLSP--------F 169
            +++ A+   G +++    +L  A +   AG+D ++     AGGH G   P        F
Sbjct: 141 ADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGGHRGIFDPTKDEQIGSF 200

Query: 170 ALVGEVRKMFKGPLALSGSIATGDAVLAAQAMGADFAYMGSRWLATRESNVDDAYRQAIV 229
           AL+  + +  + P+  +G I  G  + AA  +GA    MG+ +L TRESN + +YR A++
Sbjct: 201 ALLRLLVQHTRLPVIAAGGIMDGHGIAAALQLGAQAVQMGTAFLLTRESNANASYR-ALL 259

Query: 230 ESTAADVVYTNLFTGVHGNYLKKSIVNAGLDP-DNLPEADKTKMNFGSGGGSKAKAW--- 285
           +S  A   +T +   + G    + IVN  ++  D                G +  A    
Sbjct: 260 KSPRAQ--HTRVTAAISGRP-ARGIVNRFMEEIDTPAAPPIPAYPAAYDAGKQLHALAAA 316

Query: 286 RDI------WGAGQGVGLMEDVPTVAEMVDRLEEEYRAARA 320
           RD+      W AGQG  L+ D  TVAE+V  L +E++A +A
Sbjct: 317 RDVHEFGAHW-AGQGAALIRDGYTVAELVHLLVQEWQAQQA 356


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.133    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,110,134
Number of extensions: 49545
Number of successful extensions: 220
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 216
Number of HSP's successfully gapped: 3
Length of query: 328
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 242
Effective length of database: 1,212,660
Effective search space: 293463720
Effective search space used: 293463720
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 131 (55.1 bits)