BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0037.p (199 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313980|ref|YP_003778072.1| two component response regulato... 358 e-100 gi|300310565|ref|YP_003774657.1| response regulator containing a... 112 2e-26 gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 101 4e-23 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 100 1e-22 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 99 2e-22 >gi|300313980|ref|YP_003778072.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 199 Score = 358 bits (919), Expect = e-100, Method: Compositional matrix adjust. Identities = 171/199 (85%), Positives = 183/199 (91%) Query: 1 MPVSEPNKIYQEVIDALIALHRLTDGYTPNHEDQTAELAVMIGRHLALPAERIDVLHMAA 60 MPVSE NKIYQEVIDA+IA+HRLTDGYTPNHEDQTAELAVMIGRHL L ERID LHMA+ Sbjct: 1 MPVSESNKIYQEVIDAMIAVHRLTDGYTPNHEDQTAELAVMIGRHLDLATERIDALHMAS 60 Query: 61 QVHDIGKARIPLDLLTKPGRITADEYGVLKTHVQFGYEILNKISFPFNLAEIVWCHHEYL 120 QVHDIGKARIPL LLTKPGRI+ +EY VLKTHVQFGYEILNKISFPFNLAEIV CHHEYL Sbjct: 61 QVHDIGKARIPLALLTKPGRISVEEYDVLKTHVQFGYEILNKISFPFNLAEIVHCHHEYL 120 Query: 121 DGSGYPRGIGGDALPLEARILTVADIVESMSADRPYRKSLGMAVALEEIQRQSGSRLDPV 180 DGSGYPRGI GD +PLEARILTVADIVESMSADRPYRKSLGM+ AL+EIQRQSG RLD V Sbjct: 121 DGSGYPRGISGDEIPLEARILTVADIVESMSADRPYRKSLGMSAALDEIQRQSGVRLDAV 180 Query: 181 VVDACLHVVQQHNWRPVTN 199 VVDACL VV++ NWRP++N Sbjct: 181 VVDACLDVVKRSNWRPISN 199 >gi|300310565|ref|YP_003774657.1| response regulator containing a CheY-like receiver domain and an HD-GYP domain [Herbaspirillum seropedicae SmR1] Length = 348 Score = 112 bits (280), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 62/184 (33%), Positives = 101/184 (54%), Gaps = 2/184 (1%) Query: 3 VSEPNKIYQEVIDALIALHRLTDGYTPNHEDQTAELAVMIGRHLALPAERIDVLHMAAQV 62 V + + + +D L D T H + A A +I + L +++ ++L AA + Sbjct: 146 VRHSDTLALDSVDLLARAAGYRDPETGEHLTRMANYARIIAARMGLDSQQQELLLNAAPM 205 Query: 63 HDIGKARIPLDLLTKPGRITADEYGVLKTHVQFGYEILNKISFPF--NLAEIVWCHHEYL 120 HDIGK IP +L KPGR+ +E V++ H + G +IL P ++I HHE Sbjct: 206 HDIGKMGIPDHILLKPGRLDEEELKVMRMHARIGADILVASPSPLLQTASQIALTHHEKW 265 Query: 121 DGSGYPRGIGGDALPLEARILTVADIVESMSADRPYRKSLGMAVALEEIQRQSGSRLDPV 180 DGSGYP G+ G+ +PL RI+ VAD+ +++++ RPY+ + A + ++ QSG+ DP Sbjct: 266 DGSGYPNGLKGEQIPLYGRIVAVADVFDALTSSRPYKPGWSLERASQYLREQSGAHFDPR 325 Query: 181 VVDA 184 V+A Sbjct: 326 CVEA 329 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 101 bits (252), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 54/178 (30%), Positives = 99/178 (55%), Gaps = 4/178 (2%) Query: 15 DALIALHRL--TDGYTPNHEDQTAELAVMIGRHLALPAERIDVLHMAAQVHDIGKARIPL 72 DALI+L RL TD YT H + + + L + ++ MA +HD+GK +PL Sbjct: 147 DALISLARLKTTDDYTYLHSVAVCAMMISLANQLGMTPQQTKQAGMAGLLHDVGKMAVPL 206 Query: 73 DLLTKPGRITADEYGVLKTHVQFGYEILNKISFPFNLA-EIVWCHHEYLDGSGYPRGIGG 131 D+L KP ++T +E+ ++ H G+ IL +I +A ++ HHE +DGSGYP + Sbjct: 207 DILNKPAKLTEEEFASVRMHTVQGHGILQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKA 266 Query: 132 DALPLEARILTVADIVESMSADRPYRKSLGMAVALEEIQRQSGSRLDPVVVDACLHVV 189 D++ + A++ V D+ ++++++RPY+ A ++ + +G DP+ + A + + Sbjct: 267 DSISIMAKMGAVCDVYDAITSNRPYKAGWDPARSIRHMAASAG-HFDPITMQAFVKAI 323 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 99.8 bits (247), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 50/154 (32%), Positives = 88/154 (57%), Gaps = 1/154 (0%) Query: 22 RLTDGYTPNHEDQTAELAVMIGRHLALPAERIDVLHMAAQVHDIGKARIPLDLLTKPGRI 81 +L D YT H L V +GR L L + +HD+GKA IP +LL KPG++ Sbjct: 153 KLADEYTYLHSVAVCALMVALGRTLGLDETACREAGLGGFLHDVGKAFIPHELLNKPGKL 212 Query: 82 TADEYGVLKTHVQFGYEILNKI-SFPFNLAEIVWCHHEYLDGSGYPRGIGGDALPLEARI 140 + +E+ +K H + GY+ L++ + P ++ HHE +DG+GYP G+ A+ L +R+ Sbjct: 213 SDEEFSQIKKHPELGYKYLSQDPNVPDYARDVCLHHHEKIDGNGYPEGLRAGAITLYSRM 272 Query: 141 LTVADIVESMSADRPYRKSLGMAVALEEIQRQSG 174 + D+ +++++DRPY+++ A A+ + +G Sbjct: 273 AAICDVYDALTSDRPYKRAWDPAEAISAMASWTG 306 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 99.4 bits (246), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 50/154 (32%), Positives = 87/154 (56%), Gaps = 1/154 (0%) Query: 22 RLTDGYTPNHEDQTAELAVMIGRHLALPAERIDVLHMAAQVHDIGKARIPLDLLTKPGRI 81 +L D YT H L + +GR L L ++ +HD+GKA I ++L KPG++ Sbjct: 150 KLNDEYTYLHSVSVCALVIGLGRTLGLSEAACREAGLSGYLHDVGKAFIDDEILNKPGKL 209 Query: 82 TADEYGVLKTHVQFGYEILNKI-SFPFNLAEIVWCHHEYLDGSGYPRGIGGDALPLEARI 140 TA+E+ ++K H + GY+ L + P +++ HHE LDG GYP + +++ L AR+ Sbjct: 210 TAEEFALIKRHPELGYQYLIQTPDMPAYASDVCRHHHERLDGRGYPDALPAESITLYARM 269 Query: 141 LTVADIVESMSADRPYRKSLGMAVALEEIQRQSG 174 V D+ ++++++RPY+ A A+ ++ R G Sbjct: 270 TAVCDVYDALTSERPYKHGWDPAEAISQMARWEG 303 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 663,967 Number of extensions: 27456 Number of successful extensions: 86 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 78 Number of HSP's successfully gapped: 11 Length of query: 199 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 118 Effective length of database: 1,236,335 Effective search space: 145887530 Effective search space used: 145887530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)