BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0031.p (546 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid li... 1073 0.0 gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA lig... 226 2e-60 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 162 6e-41 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 134 2e-32 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 132 8e-32 >gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 1073 bits (2775), Expect = 0.0, Method: Compositional matrix adjust. Identities = 527/546 (96%), Positives = 536/546 (98%) Query: 1 MADFDTGLGRNAANYAALTPIDFIARAAAVYGQRPAIIHGALRQDWDQTYRRTRRLASAL 60 MADFDTGLGRNAANYAALTPIDFIARAAAVYG+R AIIHGALRQDWDQTYRRTRRLASAL Sbjct: 1 MADFDTGLGRNAANYAALTPIDFIARAAAVYGKRTAIIHGALRQDWDQTYRRTRRLASAL 60 Query: 61 QRLGIGKNDTVSAMLPNTPAMVEAHFGVPMAGAVLNALNIRLDAESIVFMLRHGEAKVLL 120 Q LG+GKNDTVSAMLPNTPAMVEAHFGVPMAGAVLNALNIRLDAESIVFMLRHGEAKVLL Sbjct: 61 QGLGVGKNDTVSAMLPNTPAMVEAHFGVPMAGAVLNALNIRLDAESIVFMLRHGEAKVLL 120 Query: 121 IDSEFAALAQQLRTQLPALKIVEVFDVLGPPPVPGERFGHLEYEALLADGDENFEWQWPA 180 IDSEFAALAQQLRTQLPALKIVEVFD LGPPPV GERFGHLEYEALLA GDE+F+WQ PA Sbjct: 121 IDSEFAALAQQLRTQLPALKIVEVFDELGPPPVAGERFGHLEYEALLAGGDEHFDWQMPA 180 Query: 181 DEWDAIALNYTSGTTGDPKGVVYHHRGAALNAVSNILEWDLPKHPVYLWTLPMFHCNGWC 240 DEWDAIALNYTSGTTGDPKGVVYHHRGAALNAVSNILEWDLPKHPVYLWTLPMFHCNGWC Sbjct: 181 DEWDAIALNYTSGTTGDPKGVVYHHRGAALNAVSNILEWDLPKHPVYLWTLPMFHCNGWC 240 Query: 241 FPWTVAARAGVNVCLRKFDPKLVFDLIAEHGITHYCAAPIVHAALANAPEGWRAGIRGPV 300 FPWTVAARAGVNVCLRKF+PKLVFDLIAEHGITHYCAAPIVHAALANAPEGWRAGIRGPV Sbjct: 241 FPWTVAARAGVNVCLRKFEPKLVFDLIAEHGITHYCAAPIVHAALANAPEGWRAGIRGPV 300 Query: 301 KAMVAGAPPPAAVLAKMEAMQFELTHVYGLTEVYGPAAVCAEQDEWSTLSVDQRAVQKSR 360 KAMVAGAPPPAAVLAKMEAMQFELTHVYGLTEVYGPAAVCAEQDEW+TLSVDQRAVQKSR Sbjct: 301 KAMVAGAPPPAAVLAKMEAMQFELTHVYGLTEVYGPAAVCAEQDEWNTLSVDQRAVQKSR 360 Query: 361 QGVRYHLQSGVTVLSPDTMEPVAADGEEIGEIMFRGNICMKGYLKNDKATHEAFAGGWFH 420 QGVRYHLQSGVTVLSPDTM PVAADGEEIGEIMFRGNICMKGYLKND+ATHEAFAGGWFH Sbjct: 361 QGVRYHLQSGVTVLSPDTMAPVAADGEEIGEIMFRGNICMKGYLKNDRATHEAFAGGWFH 420 Query: 421 TGDLGVMNADGYIKIKDRSKDIIISGGENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGE 480 TGDLGVMNADGYIKIKDRSKDIIISGGENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGE Sbjct: 421 TGDLGVMNADGYIKIKDRSKDIIISGGENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGE 480 Query: 481 TPCAFVELKDGANIDAAELTEFCRGHLAGFKVPKAIYFGPLPKTSTGKIQKFELRKRMKS 540 TPCAFVELK+GA + AAELTEFCR HLAGFKVPKAIYFGPLPKTSTGKIQKFELRKRMKS Sbjct: 481 TPCAFVELKEGAEVGAAELTEFCRSHLAGFKVPKAIYFGPLPKTSTGKIQKFELRKRMKS 540 Query: 541 DSAINV 546 DSAINV Sbjct: 541 DSAINV 546 >gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA ligase [Herbaspirillum seropedicae SmR1] Length = 545 Score = 226 bits (577), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 175/521 (33%), Positives = 265/521 (50%), Gaps = 27/521 (5%) Query: 37 IIHGALRQDWDQTYRRTRRLASALQRLGIGKNDTVSAMLPNTPAMVEAHFGVPMAGAVLN 96 II+G R + Y R +RLA+AL+ +G+ +TV+ M ++ +E F VPM G VL Sbjct: 34 IIYGQRRLSYAGFYERVQRLANALKEMGLQPGNTVAVMDWDSNRYLECFFAVPMMGCVLQ 93 Query: 97 ALNIRLDAESIVFMLRHGEAKVLLIDSEFAALAQQLRTQLPAL-KIVEVFDVLGPPPVPG 155 +N+RL E I + L H +A VLL++S+F L +Q+R +L L + V + D P P G Sbjct: 94 TVNVRLSPEQIAYTLNHAQADVLLVNSDFLPLLKQIRGELTTLTRCVWLTDDATPCP-RG 152 Query: 156 ERFGHLEYEALLADGDENFEWQWPADEWDAIALNYTSGTTGDPKGVVYHHRGAALNAVSN 215 F EYE LLA + ++ DE YT+GTTG PKGV + HR L+ ++ Sbjct: 153 PGFAG-EYEELLAQAEPACDFP-DFDENARATTFYTTGTTGLPKGVYFSHRQLVLHTLAT 210 Query: 216 ILEWDLP-------KHPVYLWTLPMFHCNGWCFPWTVAARAGV-NVCLRKFDPKLVFDLI 267 L + VY+ PMFH + W P+ VA AGV V ++ P + LI Sbjct: 211 TAGLALAPQQGRFHRDDVYMPLTPMFHVHAWGLPY-VATMAGVKQVYPGRYVPDTICRLI 269 Query: 268 AEHGITHYCAAPIVHAALANAPEGWRAGIRGPVKAMVAGAPPPAAVLAKMEAMQ-FELTH 326 E +T P + + + + A + G M+ G LAKM + ++ Sbjct: 270 REEKVTFSHCVPTILQMVLTSEQAREADLSG--WKMIIGGSAMTGSLAKMARDRGIDVFT 327 Query: 327 VYGLTEVYGPAAVCAEQDEWSTLSVDQRAVQKSRQGVRYHLQSGVTVLSPDTMEPVAADG 386 YG++E P A Q L ++ A + + G L TV M+ DG Sbjct: 328 GYGMSETC-PVLTLA-QVPTEELGGEREAAIRVKTGRPLPLVDLRTV--DGEMQDCPRDG 383 Query: 387 EEIGEIMFRGNICMKGYLKNDKATHEAFAGGWFHTGDLGVMNADGYIKIKDRSKDIIISG 446 GE++ R +GYL +A+ E + GGW HT D+G ++A+GY+++ DR KD+I +G Sbjct: 384 STTGEVVVRAPWLTQGYLHQAEASEELWRGGWLHTQDIGHLDAEGYLQVTDRIKDVIKTG 443 Query: 447 GENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGETPCAFVEL-KDGANIDAAELTEFCR- 504 GE +SS+++ED++ RHPAV AV+ D KWGE P A V L KD A + ++ E R Sbjct: 444 GEWVSSLQIEDIIARHPAVAETAVIGIQDGKWGERPVALVVLKKDAAAVSEDDIREHVRA 503 Query: 505 ----GHLAGFKVPKAIYF-GPLPKTSTGKIQKFELRKRMKS 540 G ++ + VP + F L +TS GK+ K +R++ S Sbjct: 504 AAEAGAISRYGVPDRVRFVAELARTSVGKLNKRVMREQYPS 544 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 162 bits (410), Expect = 6e-41, Method: Compositional matrix adjust. Identities = 141/499 (28%), Positives = 215/499 (43%), Gaps = 27/499 (5%) Query: 46 WDQTYRRTRRLASALQRLGIGKNDTVSAMLPNTPAMVEAHFGVPMAGAVLNALNIRLDAE 105 W + R LA AL R G+ K D + N + E+ + G V N R+ Sbjct: 65 WREINARVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFKMGVVWVPTNFRITPP 124 Query: 106 SIVFMLRHGEAKVLLIDSEFAALAQQLRTQLPALK-IVEVFDVLGPPPVPGERFGHLEYE 164 ++ + A V+L D FA ++ PAL+ +V + D R G L+YE Sbjct: 125 EAAYLGQSSGACVMLYDRGFAQYVDAVKAASPALEHVVALADA---------RAGELDYE 175 Query: 165 ALLADGDENFEWQWPADEWDAIALNYTSGTTGDPKGVVYHHRGAALNAVSNILEW--DLP 222 AL+ +G + D D + YTSGTTG PK + H A +++ + L Sbjct: 176 ALVREGSGAAFREAEVDYDDPLWFFYTSGTTGHPKAGMLSHGQMAFVVTNHLADLMPGLT 235 Query: 223 KHPVYLWTLPMFHCNGWCFPWTVAARAGVNVCL--RKFDPKLVFDLIAEHGITHYCAAPI 280 L P+ H G AR ++ L + + + L+ ++ + + P Sbjct: 236 HQSRSLVVAPLSHGAG-VHAVINTARGAASILLSSERLVVEEAWQLVEKYRVDNLFTVPT 294 Query: 281 VHAALANAPEGWRAGIRGPVKAMVAGAPP-PAAVLAKMEAMQFELTHVYGLTEVYGPAAV 339 + L R + AGAP A + ++ + L YGL EV G Sbjct: 295 IVKILVEDESVDRYDHSSLKHVIYAGAPMYRADQVYALKKLGKVLVQYYGLGEVTGNITF 354 Query: 340 CAEQDEWSTLSVDQRAVQKSRQGVRYHLQSG--VTVLSPDTMEPVAADGEEIGEIMFRGN 397 L + A ++R G ++G + +L+ D E E GEI RG Sbjct: 355 LP-----PYLHFEDDAHPQARVGTCGMPRTGMQIAILNDDGQELAPF---ETGEICVRGP 406 Query: 398 ICMKGYLKNDKATHEAFAGGWFHTGDLGVMNADGYIKIKDRSKDIIISGGENISSVEVED 457 GY N +A +AF WFHTGDLG ++ DG++ I RS D+ ISGG N+ E+E+ Sbjct: 407 AVFMGYHNNPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSSDMYISGGSNVYPREIEE 466 Query: 458 VLYRHPAVLAAAVVAQPDEKWGETPCAFVELKDGANIDAAELTEFCRGHLAGFKVPKA-I 516 L HPAV AV+ PD KWGE+ A + K G +D L + +K P+ I Sbjct: 467 ALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAGQQVDGEALLAHLESRIGKYKWPRRFI 526 Query: 517 YFGPLPKTSTGKIQKFELR 535 Y+ +PK+ GKI K +++ Sbjct: 527 YWDSMPKSGYGKIVKKQIK 545 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 134 bits (336), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 119/369 (32%), Positives = 169/369 (45%), Gaps = 37/369 (10%) Query: 184 DAIALNYTSGTTGDPKGVVYHHRGAALNAVSNIL---EWDLP--------KHPVYLWTLP 232 D L YT GTTG KG V HR N V+N+L EW P V++ LP Sbjct: 206 DIAFLQYTGGTTGVAKGAVLTHR----NLVANVLQAEEWLTPALKAGKPIDQLVFVCALP 261 Query: 233 MFHCNGWCFPWTVAARAG-VNVCLR--KFDPKLVFDLIAEHGITHYCAAPIVHAALANAP 289 ++H + R G +N+ + + P V +L A++ + + A ++ AL N Sbjct: 262 LYHIFALTVCNMLGTREGALNLLIPNPRDIPGFVKEL-AKYKVNTFPAVNTLYNALLNNA 320 Query: 290 EGWRAGIRGPVKAMVAGAPPPAAVLAKMEAMQ-FELTHVYGLTEVYGPAAVCAEQDEWST 348 + R G + G +V K + + + YGL+E P A Sbjct: 321 DFARLDFSGYRCCVGGGMAVQKSVADKWKKLTGCPIIEGYGLSET-SPIA---------- 369 Query: 349 LSVDQRAVQKSRQGVRYHLQSGVTVLSPDTMEPVAADGEEIGEIMFRGNICMKGYLKNDK 408 S + + + + L S + D +PV + GEI RG M GY + Sbjct: 370 -SANPCTIDEYTGTIGLPLPSTEFAILDDDGQPVPLG--QPGEIAIRGPQVMPGYWQRPD 426 Query: 409 ATHEAFA-GGWFHTGDLGVMNADGYIKIKDRSKDIIISGGENISSVEVEDVLYRHPAVLA 467 T + G+F TGD+GVM+ GY KI DR KD+I+ G N+ EVE V+ +HP VL Sbjct: 427 ETAKVMTPDGFFKTGDIGVMDERGYTKIVDRKKDMILVSGFNVYPNEVEGVVAQHPGVLE 486 Query: 468 AAVVAQPDEKWGETPCAFVELKDGANIDAAELTEFCRGHLAGFKVPKAIYF-GPLPKTST 526 A V PDE GE FV KD + AA+L ++C+ G+K PK I F LPKT+ Sbjct: 487 CACVGVPDEHTGEAVKLFVVRKD-KTLSAAQLMDYCKEQFTGYKKPKYIEFRDELPKTNV 545 Query: 527 GKIQKFELR 535 GKI + ELR Sbjct: 546 GKILRRELR 554 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 132 bits (332), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 152/520 (29%), Positives = 210/520 (40%), Gaps = 67/520 (12%) Query: 46 WDQTYRRTRRLASALQRLGIGKNDTVSAMLPNTPAMVEAHFGVPMAGAVLNALNIRLDAE 105 +DQ + R A+AL+ G+G+ D + L V H GA+ L + Sbjct: 65 YDQLKILSDRFANALRAAGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPD 124 Query: 106 SIVFMLRHGEAKVLLIDSEFAALAQQLRTQLPALKIV---EVFDVLGPPPVPGERFGHL- 161 +I + L + A L+ D+ A L +LPAL++V E D++ P H Sbjct: 125 AIAYRLDNSGAVALVTDASGMEKAT-LAGELPALRLVFCVESSDLVLPQTTDFWDRLHAA 183 Query: 162 --EYEALLADGDENFEWQWPADEWDAIALNYTSGTTGDPKGVVYHHRGAALNAVSNILEW 219 E + +L D D + YTSGTTG KG ++ HR +L Sbjct: 184 PAELDPVLTLAD------------DPAMIIYTSGTTGKAKGALHAHR---------VLLG 222 Query: 220 DLPKHPVYLWTLPMFHCNGWC--------------FPWTVAARAGVNVCLRKFDPKLVFD 265 LP V + P W P A V L KFD VF Sbjct: 223 HLPGVEVSHDSFPQEGDRFWTPADWAWIGGLLDVLLPSLYHGVAVVARRLEKFDAAEVFG 282 Query: 266 LIAEHGITHYCAAPIVHAALANAPEGWRAGIRGPVKAMVAGAPPPAAVLAK--MEAMQFE 323 L+A H I + P L A RA ++++ +G L EA+ Sbjct: 283 LLARHQIRNVFFPPTALKMLRGAAT-VRAQADFSLRSVASGGETLGDDLIAWGREALGVT 341 Query: 324 LTHVYGLTEVYGPAAVCAEQDEWSTLSVDQ-RAVQKSRQGVRYHLQSGVTVLSPDTMEPV 382 + YG TE V + + ++S RAV G V D V Sbjct: 342 INEFYGQTECN--LVVSSSSHCYPSVSGSMGRAV------------PGHVVQIVDEQGQV 387 Query: 383 AADGEEIGEIMFRGN--ICMKGYLKNDKATHEAFAGGWFHTGDLGVMNADGYIKIKDRSK 440 G +G I R + Y +N++AT E FAG + TGDLG M+ GYI+ R+ Sbjct: 388 LPHGT-VGNIAIRAPDPVMFLRYWRNEEATREKFAGDFLLTGDLGSMDEQGYIRYLGRND 446 Query: 441 DIIISGGENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGETPCAFVELKDGANID---AA 497 D+I S G I +E+ L RHPAV AAVV D E AFV LKDG D A Sbjct: 447 DVITSAGYRIGPAAIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDGVTPDEALKA 506 Query: 498 ELTEFCRGHLAGFKVPKAIYF-GPLPKTSTGKIQKFELRK 536 EL + R LA + P+ I F LP T+TGKI + L++ Sbjct: 507 ELQQHVRAQLAAHEYPRLISFVAALPTTATGKIMRKTLKE 546 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,723,606 Number of extensions: 72458 Number of successful extensions: 273 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 247 Number of HSP's successfully gapped: 13 Length of query: 546 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 456 Effective length of database: 1,193,720 Effective search space: 544336320 Effective search space used: 544336320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)