BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0025.p (407 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313991|ref|YP_003778083.1| major facilitator superfamily p... 697 0.0 gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance... 112 6e-26 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 80 3e-16 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 64 2e-11 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 61 1e-10 >gi|300313991|ref|YP_003778083.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 406 Score = 697 bits (1799), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/405 (91%), Positives = 388/405 (95%), Gaps = 1/405 (0%) Query: 1 MHNKDTSLSMGKFPPWLAVLAALTALGALAIDMYLPSFTAIAKELGVGTNLVQLTLASFL 60 M N DTSLS GKFP WLAVLA LTA+GALAIDMYLPSFTAIA++LGVGTN VQLTLA+FL Sbjct: 1 MQNNDTSLSTGKFPAWLAVLAGLTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFL 60 Query: 61 VGLAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLLCIFVQSIETLIVLRLFQGLGGSAG 120 +G+AVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLLCIF Q+IETLIVLRLFQGLGGSAG Sbjct: 61 IGVAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAG 120 Query: 121 MVIPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTIFGL 180 MVIPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLT+FGL Sbjct: 121 MVIPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTLFGL 180 Query: 181 LCMLGTRKYLTETVDVSRAEPLHLGRTLRQYWGLLRHRQFMSFVLCGGLVQAGMFAYIAG 240 LC+LGTRKYLTETVDVSRAEPLHLGRTLRQYWGLLRHRQFMS+VLCGGLVQAGMFAYIAG Sbjct: 181 LCILGTRKYLTETVDVSRAEPLHLGRTLRQYWGLLRHRQFMSYVLCGGLVQAGMFAYIAG 240 Query: 241 GPFVVIELHGIKPQYFGFVFASNAIGLIAASQINARLVVKRSAERVLGKVLWVPAGLSLA 300 GPFVVIELHG+KPQYFGFVFASNAIGLIAASQ+NARLVVKRSAERVLGKVLWVPAGLSL Sbjct: 241 GPFVVIELHGVKPQYFGFVFASNAIGLIAASQVNARLVVKRSAERVLGKVLWVPAGLSLT 300 Query: 301 VVLAVLAGLESLPLLLFGFFGFLTAYGFTGPNATAIALKHQGRQAGTAAALMGTLQFGMG 360 V L VL GLE+LPLLL GFFGFLTAYGFTGPNATAIALKHQGRQAGTAAALMG+LQFGMG Sbjct: 301 VSLLVLVGLETLPLLLLGFFGFLTAYGFTGPNATAIALKHQGRQAGTAAALMGSLQFGMG 360 Query: 361 VLSGVVMSLWHDGTALPLVSVMAICGVSAFLLYHFVAKHEP-VTP 404 VLSGV MSLWHDGTALPLVSVMA+CGV+A L+YHF AKH P V+P Sbjct: 361 VLSGVAMSLWHDGTALPLVSVMAVCGVTALLVYHFAAKHAPAVSP 405 >gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance transporter protein [Herbaspirillum seropedicae SmR1] Length = 417 Score = 112 bits (280), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 77/272 (28%), Positives = 136/272 (50%), Gaps = 8/272 (2%) Query: 19 VLAALTALGALAIDMYLPSFTAIAKELGVGTNLVQLTLASFLVGLAVGQMFYGPLSDRFG 78 ++ A + G LAID+Y+P+ +A + +Q ++ +L+ A GQ+ +GPL+D +G Sbjct: 25 LICAFASAGQLAIDLYVPALPEMAIDYATSAQAIQSSVTGYLIAYAFGQLVFGPLADAYG 84 Query: 79 RKPPLFFGIALYFVCSLLCIFVQSIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEGSA 138 RK L G+ L+ + +L + ++ET + R+ QG G +A ++ +A++ D + Sbjct: 85 RKRILALGLGLFSLGCVLSLAAPNLETFVAARVLQGFGIAATNLLAKAIITDSFAGQALV 144 Query: 139 KAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTIFGLLCMLGTRKYLTETVDVSR 198 A++ + + +GLAPI+AP IG + WR V L ++ L +Y R Sbjct: 145 HAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLLVYSLGMWAVLWRYRETLAQPVR 204 Query: 199 AEPLHLGRTLRQYWGLLRHRQFMSFVLCGGLVQAGMFAYIAGGPFVVIELHGIKPQYFGF 258 P L R R +L F S L GL + + + GPF+V P +FG+ Sbjct: 205 LHPATLARNARM---VLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTLHQPPTFFGY 261 Query: 259 VFASNAIGLIA-ASQINARLVVKR--SAERVL 287 + + AIG++ I+ R+ +R +AE+ L Sbjct: 262 L--ALAIGMMYFLGGISNRITHRRLPTAEQRL 291 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 80.1 bits (196), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 57/202 (28%), Positives = 98/202 (48%), Gaps = 1/202 (0%) Query: 3 NKDTSLSMGKFPPWLAVLAALTALGALAIDMYLPSFTAIAKELGVGTNLVQLTLASFLVG 62 D SL+ + ++AA + L + + +A+ GVG N + L + ++++ Sbjct: 19 TNDNSLTDARVRMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLT 78 Query: 63 LAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLLCIFVQSIETLIVLRLFQGLGGSAGMV 122 LAV G ++DRFG + IA++ + S+LC F Q++ R+ QGLGG+ + Sbjct: 79 LAVFIPISGWVADRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVP 138 Query: 123 IPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTIFGLLC 182 + R +V G + KA + + +AP++ P IGG + +ASW IF++ FGL Sbjct: 139 VGRMIVVRSTGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAA 198 Query: 183 MLGTRKYLTETVDVSRAEPLHL 204 + VS+ PL L Sbjct: 199 FTAVMMVVRNEHGVSK-RPLDL 219 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 63.9 bits (154), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 47/175 (26%), Positives = 88/175 (50%), Gaps = 4/175 (2%) Query: 22 ALTALGALAIDMYLPSFTAIAKELGVGTNLVQLTLASFLVGLAVGQMFYGPLSDRFGRKP 81 A+T L + + LP+ +A++LG + ++ ++S+++ A + G +DR+GR+ Sbjct: 46 AMTVLDTNVVGIILPT---VARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYGRRR 102 Query: 82 PLFFGIALYFVCSLLCIFVQSIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEGS-AKA 140 L GIAL+ + SL C S + L R QG+G + + A++ E +A Sbjct: 103 VLLGGIALFALTSLACALAPSAQALYGARALQGVGAAFLLAPALAVIGHAFHDEDQRERA 162 Query: 141 FSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTIFGLLCMLGTRKYLTETVD 195 +++ + GL +LAP IGG++ WR F V LL + +++ E+ + Sbjct: 163 WALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHVPESRN 217 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 49/171 (28%), Positives = 81/171 (47%), Gaps = 7/171 (4%) Query: 37 SFTAIAKELGVGTNLVQLTLASFLVGLAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLL 96 S A+A ++ V ++L L S+LV LAV G ++DRFG I ++ + S+L Sbjct: 9 SLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAICVFMLGSIL 68 Query: 97 CIFVQSIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAP 156 C ++ + R QG+GG+ + + R ++ + KA S L + P+L P Sbjct: 69 CGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPALFGPVLGP 128 Query: 157 FIGGLMLVFASWRGIFVV---LTIFGLLCMLGTRKYLTETVDVSRAEPLHL 204 +GG + + WR IF + ++I GL L TR + + PL L Sbjct: 129 PLGGFITTYFHWRWIFFINIPISILGL--YLATR--FIPDLKAEQVAPLDL 175 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.144 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,110,524 Number of extensions: 45148 Number of successful extensions: 307 Number of sequences better than 1.0e-08: 23 Number of HSP's gapped: 302 Number of HSP's successfully gapped: 23 Length of query: 407 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 320 Effective length of database: 1,207,925 Effective search space: 386536000 Effective search space used: 386536000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)