BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0007.p (475 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314008|ref|YP_003778100.1| glycerol-3-phosphate dehydrogen... 945 0.0 gi|300310299|ref|YP_003774391.1| glycerol-3-phosphate dehydrogen... 587 e-168 >gi|300314008|ref|YP_003778100.1| glycerol-3-phosphate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 534 Score = 945 bits (2442), Expect = 0.0, Method: Compositional matrix adjust. Identities = 458/471 (97%), Positives = 463/471 (98%) Query: 1 VALCEQHDLAQHTSSSSSKLIHGGLRYLEHYEFGLVRKALIEREVLLRSAPHIMWPLRFV 60 VALCEQHDLAQHTSSSSSKLIHGGLRYLEHYEFGLVRKALIEREVLLRSAPHIMWPLRFV Sbjct: 40 VALCEQHDLAQHTSSSSSKLIHGGLRYLEHYEFGLVRKALIEREVLLRSAPHIMWPLRFV 99 Query: 61 MPHDQGQRPAWLIRLGMFLYDHLARRELLPGSRALDLRRHVSGAPLKAQFTRGFVYSDGW 120 MPHDQGQRPAWLIRLGMFLYDHLARRELLPGSRALDLRRH SGAPLKAQFTRGFVYSDGW Sbjct: 100 MPHDQGQRPAWLIRLGMFLYDHLARRELLPGSRALDLRRHASGAPLKAQFTRGFVYSDGW 159 Query: 121 VDDARLVVLNAMDAAEKGAHIMTRTRCESLTRQDNGWQAVLRREDGHAMQVRARCVVNAA 180 VDDARLVVLNAMDAAEKGAHIMTRTRCESLTRQDNGWQAVLRREDGHAMQVRARCVVNAA Sbjct: 160 VDDARLVVLNAMDAAEKGAHIMTRTRCESLTRQDNGWQAVLRREDGHAMQVRARCVVNAA 219 Query: 181 GPWVAQFLGGAAHVHSSKSVRLVKGSHIVVPRLFEHDHAYIFQNPDGRIVFAIPYEQDFT 240 GPWVAQFLGGAAHVHSSKSVRLVKGSHIVVPRLFEHDHAYIFQNPDGRIVFAIPYEQDFT Sbjct: 220 GPWVAQFLGGAAHVHSSKSVRLVKGSHIVVPRLFEHDHAYIFQNPDGRIVFAIPYEQDFT 279 Query: 241 LIGTTDIEYQGRVEDVAISEDETAYLCQLANRYFDKPITPADVVWSYSGVRPLLEDAAAD 300 LIGTTDIEYQGRVEDVAIS DETAYLCQLANRYF+KPITP DVVWSYSGVRPLLEDAAAD Sbjct: 280 LIGTTDIEYQGRVEDVAISADETAYLCQLANRYFEKPITPDDVVWSYSGVRPLLEDAAAD 339 Query: 301 ASAVTRDYQLDLNTQGAPLLSIFGGKITTFRKLAEEAVDMIAPVLGNQRGAWTEHGCLPG 360 ASAVTRDYQLDLN QGAPLLSIFGGKITTFRKLAEEAVDMIAPVLGNQRGAWTEHGCLPG Sbjct: 340 ASAVTRDYQLDLNLQGAPLLSIFGGKITTFRKLAEEAVDMIAPVLGNQRGAWTEHGCLPG 399 Query: 361 GDLYGAQPSNRSVRQFGHYVEGLQQQYPWLPAALLQRYARAYGSRTHALLEGRKNLEEMG 420 GDLYGAQPSNRSVRQF YV+GLQQQYPWLPAALLQRYARAYGSRTH LLEGR+N+EEMG Sbjct: 400 GDLYGAQPSNRSVRQFDQYVQGLQQQYPWLPAALLQRYARAYGSRTHTLLEGRQNVEEMG 459 Query: 421 EEILGGLYAAEVDYLLRHEWATNAADILWRRSKLGLHFPKGSEAVLDAWLA 471 EEIL GLYAAEVDYLLRHEWA+NAADILWRRSKLGLHFPKGSEAVLDAWLA Sbjct: 460 EEILDGLYAAEVDYLLRHEWASNAADILWRRSKLGLHFPKGSEAVLDAWLA 510 >gi|300310299|ref|YP_003774391.1| glycerol-3-phosphate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 510 Score = 587 bits (1512), Expect = e-168, Method: Compositional matrix adjust. Identities = 292/475 (61%), Positives = 355/475 (74%), Gaps = 6/475 (1%) Query: 1 VALCEQHDLAQHTSSSSSKLIHGGLRYLEHYEFGLVRKALIEREVLLRSAPHIMWPLRFV 60 V LCEQHDLAQHTSS+S+KLIHGGLRYLE+YEF LVRKAL EREVLLRSAPHI+WPLRFV Sbjct: 30 VLLCEQHDLAQHTSSASTKLIHGGLRYLEYYEFKLVRKALQEREVLLRSAPHIIWPLRFV 89 Query: 61 MPHDQGQRPAWLIRLGMFLYDHLARRELLPGSRALDLRRHVSGAPLKAQFTRGFVYSDGW 120 MPHD QRPAW+IR G+F+YDHLA+RE+LP S +L RH +G PLK + +GFVYSDGW Sbjct: 90 MPHDANQRPAWMIRAGLFMYDHLAKREILPPSEGANLARHDAGQPLKGDYRKGFVYSDGW 149 Query: 121 VDDARLVVLNAMDAAEKGAHIMTRTRCESLTRQDNGWQAVL--RREDG-HAMQVRARCVV 177 VDDARLVVLNAMDA E+GA I+TRT+C S R+ + W AVL +RE G +VRAR +V Sbjct: 150 VDDARLVVLNAMDAQERGARILTRTKCISARREGDIWHAVLEHQREGGVQQEEVRARAIV 209 Query: 178 NAAGPWVAQFLGGAAHVHSSKSVRLVKGSHIVVPRLFEHDHAYIFQNPDGRIVFAIPYEQ 237 NAAGPW A+F S +S+RL+KGSHIVV R+F+H +AYIFQNPD RI+FAIPY++ Sbjct: 210 NAAGPWAARFAQAVPQAKSGRSLRLIKGSHIVVKRMFDHPYAYIFQNPDKRIIFAIPYQE 269 Query: 238 DFTLIGTTDIEYQGRVEDVAISEDETAYLCQLANRYFDKPITPADVVWSYSGVRPLLEDA 297 +FTLIGTTD+EY G V I E YLC +ANRYF + I DVVW+YSGVRPLL+D+ Sbjct: 270 EFTLIGTTDLEYHGDAGKVEIDAAEIDYLCDMANRYFKRQIGKEDVVWTYSGVRPLLDDS 329 Query: 298 AADASAVTRDYQLDLNTQGAPLLSIFGGKITTFRKLAEEAVDMIAPVLGNQRGAWTEHGC 357 A +ASA+TRDY L+ + GAP+L+++GGKITT+RKLAEEA+ M+AP LG +WT Sbjct: 330 AENASAITRDYLLECDDAGAPMLNVWGGKITTYRKLAEEALAMLAPHLGASSQSWTAGAP 389 Query: 358 LPGGDLYGAQPSNRSVR-QFGHYVEGLQQQYPWLPAALLQRYARAYGSRTHALLEGRKNL 416 LPGGDL QP R F Y+ Q ++PWLPA L RYAR+YGSR ++ +L Sbjct: 390 LPGGDL--EQPGKMVARYDFDAYLRKFQARHPWLPAELAHRYARSYGSRVEQIIGQADSL 447 Query: 417 EEMGEEILGGLYAAEVDYLLRHEWATNAADILWRRSKLGLHFPKGSEAVLDAWLA 471 +GEE+ GLY AE YL++HEWA +A DILWRRSKLGLH L+AWLA Sbjct: 448 AALGEELSPGLYEAEARYLVQHEWARSADDILWRRSKLGLHCGPQHRGRLEAWLA 502 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,583,516 Number of extensions: 67156 Number of successful extensions: 165 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 163 Number of HSP's successfully gapped: 2 Length of query: 475 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 386 Effective length of database: 1,198,455 Effective search space: 462603630 Effective search space used: 462603630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)