BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0001.p (457 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314014|ref|YP_003778106.1| two component sensor histidine ... 901 0.0 gi|300313819|ref|YP_003777911.1| two component sensor histidine ... 285 4e-78 gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase tra... 176 2e-45 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 171 2e-43 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 168 7e-43 >gi|300314014|ref|YP_003778106.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 901 bits (2328), Expect = 0.0, Method: Compositional matrix adjust. Identities = 441/457 (96%), Positives = 449/457 (98%) Query: 1 VRAYLLAWIISPIALFIVIDSFSLYRNTLESVNTAYDRMLIASVHSIGDLLRIENGELKA 60 VRAYLLAWIISPIALFI+IDSFSLYRNTLESVNTAYDRMLIASVHSIGDLLRIENGELKA Sbjct: 11 VRAYLLAWIISPIALFIIIDSFSLYRNTLESVNTAYDRMLIASVHSIGDLLRIENGELKA 70 Query: 61 SLPYAALEIYEADYSSRMIYRINGLDGKFFEGDQDLPSYKGKADKEAIYPALAHIYEDTY 120 SLPYAALEIYEADYSSRMIYRINGLDGKFF+GD DLPSYKGKADKEAIYPALAHIYEDTY Sbjct: 71 SLPYAALEIYEADYSSRMIYRINGLDGKFFDGDADLPSYKGKADKEAIYPALAHIYEDTY 130 Query: 121 NGVPVRVAALFQPVVTNDPRGAALVQVAETMENRSALARKIFWETLIRQAALLVVIVSVT 180 NGVPVRVAALFQPVVTNDPRGAALVQVAETMENRSALARKIFWETLIRQAALLVVIVSVT Sbjct: 131 NGVPVRVAALFQPVVTNDPRGAALVQVAETMENRSALARKIFWETLIRQAALLVVIVSVT 190 Query: 181 LYVVRRALQPVDALRRQLDERPADDLSPVAPPMAPRELQPFIDALNQLMARLRRLLDHQQ 240 LYVVRRALQPVDALRRQLDERPADDLSPVAPPMAPRELQPFIDALNQLMARLRRLLDHQQ Sbjct: 191 LYVVRRALQPVDALRRQLDERPADDLSPVAPPMAPRELQPFIDALNQLMARLRRLLDHQQ 250 Query: 241 RFVANASHQLRTPLAVLKTQLQSGLRGDAPAPVIWQEMSGTVERATTLANQLLSLAKVEQ 300 RFVANASHQLRTPLAVLKTQLQSGLRGDAPAPVIWQEMSGTVERATTLANQLLSLAKVEQ Sbjct: 251 RFVANASHQLRTPLAVLKTQLQSGLRGDAPAPVIWQEMSGTVERATTLANQLLSLAKVEQ 310 Query: 301 IRGRGAQEVCDLGMLARETAVDLSPLIAEKDLDFELEGEKILVMGHPWMIGELISNLLHN 360 IRGRGAQEVCDLGMLARETAVDLSPLIAEKDLDFELE EKILVMGHPWMIGELISNLLHN Sbjct: 311 IRGRGAQEVCDLGMLARETAVDLSPLIAEKDLDFELEAEKILVMGHPWMIGELISNLLHN 370 Query: 361 AIRHTPVHGKLGIRISAREDVAELLIWDSGEGIDDQAMENVFKAFSSNVSSAGGLGLTIC 420 AIRHTP G+LGIRI+AREDVAELLIWDSGEGIDDQAMENVFKAFSSNVSSAGGLGLTIC Sbjct: 371 AIRHTPAQGRLGIRITAREDVAELLIWDSGEGIDDQAMENVFKAFSSNVSSAGGLGLTIC 430 Query: 421 GEIVDSMQATISLRNRIGKDGAVEGLDALVHFRSVAG 457 GEIVDSMQATISLRNR+G+DG +EGL+A VH R VAG Sbjct: 431 GEIVDSMQATISLRNRVGEDGTIEGLNASVHVRVVAG 467 >gi|300313819|ref|YP_003777911.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 485 Score = 285 bits (730), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 168/453 (37%), Positives = 261/453 (57%), Gaps = 21/453 (4%) Query: 1 VRAYLLAWIISPIALFIVIDSFSLYRNTLESVNTAYDRMLIASVHSIGDLLRIENGELKA 60 +R+ L+ W++ P+ L + +D+ S+Y N LE + AYDR L+AS ++ + + + +G + A Sbjct: 11 LRSQLVRWLLIPLTLLVAVDAVSVYYNALEVADLAYDRSLLASTRALAERVSVVDGHVTA 70 Query: 61 SLPYAALEIYEADYSSRMIYRINGLDGKFFEGDQDLPSYKGKADKEAIYPALAHIYEDTY 120 +PY AL+ +E D R+ Y++ GL+G+ G DLP + +YPAL Y+ Y Sbjct: 71 DVPYVALDSFETDNLGRIYYKVTGLNGELVSGYDDLPPVPPDVKRSEVYPALVRFYQADY 130 Query: 121 NGVPVRVAALFQPVVTNDPRGAALVQVAETMENRSALARKIFWETLIRQAALLVVIVSVT 180 + PVR+AAL QPV + RG AL+QV ETME R+ + RKI ++TL RQ L+ V + Sbjct: 131 HDQPVRIAALLQPVYDDSIRGIALIQVGETMEGRNGMTRKILFDTLWRQGTLVTVAALLV 190 Query: 181 LYVVRRALQPVDALRRQLDERPADDLSPVAPPMAPRELQPFIDALNQLMARLRRLLDHQQ 240 + VR L+P+ L+ +++ R DLS + +E++P + A+N M RL+ L+ Q+ Sbjct: 191 WFAVRFVLRPLMQLKGEVESRAPTDLSGFDTALVHKEMRPLVQAMNGYMGRLQALISAQR 250 Query: 241 RFVANASHQLRTPLAVLKTQLQSGLR-------GDAPAPVIWQEMSGTVERATTLANQLL 293 RF+A+ASHQLRTPL VLKTQ + LR A + + M+ T + A LAN+LL Sbjct: 251 RFIADASHQLRTPLTVLKTQSELTLRELRREQVDAAELRDLVEGMARTTDAAVHLANRLL 310 Query: 294 SLAKVEQIRGRGAQEVCDLGMLARETAVDLSPLIAEKD--LDFELEGEKILVMGHPWMIG 351 +LA+ E G L AR+ A++LS ++ L FE EG+ + + + ++ Sbjct: 311 TLARAEHGAAEGGTMPLSLSDAARQVALELSQQAVSREIGLAFEEEGD-VSIEANALLLH 369 Query: 352 ELISNLLHNAIRHTPVHGKLGIRISARED-----VAELLIWDSGEGIDDQAMENVFKAF- 405 EL+ NLL NAIR+TP G + +R+ A+ VA L + DSG GI E VF+ F Sbjct: 370 ELMVNLLDNAIRYTPAGGHVTLRVRAQSSPGQPTVALLEVEDSGPGIPAAEREKVFEPFY 429 Query: 406 ----SSNVSSAG-GLGLTICGEIVDSMQATISL 433 + +V+ G GLGL+I EI +A+++L Sbjct: 430 RAGATQHVNPGGTGLGLSIVREIAAMHRASVTL 462 >gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 459 Score = 176 bits (447), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 131/431 (30%), Positives = 218/431 (50%), Gaps = 16/431 (3%) Query: 12 PIALFIVIDSFSLYRNTLESVNTAYDRMLIASVHSIGDLLRIENGELKASLPYAALEIYE 71 P+A+++ + + Y N + S DR L+AS I + +G S+P AALE++ Sbjct: 16 PMAVYVGLSTSDAYDNAVSSATLVQDRALLASARMIAGQVGWNDGAPVVSVPPAALELFA 75 Query: 72 ADYSSRMIYRINGLDGKFFEGDQDLPSYKGKADKEAIYPALAHIYEDT-YNGVPVRVAAL 130 + + Y++ D G D P+ D A+YP +Y T +NG P+RV + Sbjct: 76 SPAQDAVYYQVLMDDDTLLAGRPDFPA---APDFAALYP----VYSTTSFNGKPLRVVSY 128 Query: 131 FQPVVTNDPRGAALVQVAETMENRSALARKIFWETLIRQAALLVVIVSVTLYVVRRALQP 190 + + V V +TM+ R + + +L RQ LL++ +S+ + + L+P Sbjct: 129 IRALFDEGTLRMVAVSVGQTMQGRQEMIDDQWHASLRRQIVLLLLAISLVVLGLTMELRP 188 Query: 191 VDALRRQLDERPADDLSPVAPPMAPRELQPFIDALNQLMARLRRLLDHQQRFVANASHQL 250 + AL+ +L R D L+P+ +EL+P ++A+NQ + RL + Q+RFVA+A+HQL Sbjct: 189 LLALKDELATRRPDSLTPLRAGGLQQELRPIVEAINQYIFRLNAQVTVQKRFVADAAHQL 248 Query: 251 RTPLAVLKTQLQSGLRGDAPAPV--IWQEMSGTVERATTLANQLLSLAKVEQIRGRG-AQ 307 RTPLAV+ +Q+Q + D PA V + + T L N+LL L++ E + Sbjct: 249 RTPLAVIDSQIQFARQLDDPARVAQVLASLQEGSRSMTDLTNKLLLLSQAEASDSSAFPR 308 Query: 308 EVCDLGMLARETAVDLSPLIAEKDLDFELEG--EKILVMGHPWMIGELISNLLHNAIRHT 365 DL ++ +LS L +K +D LE E +V G+ + ++ NL+ NA+R+T Sbjct: 309 APVDLLAVSAMVLEELSGLALKKGIDLGLETTLESAMVNGNEAQLMGMLMNLIDNALRYT 368 Query: 366 PVHGKLGIRISAREDVAELLIWDSGEGIDDQAMENVFKAFSSNVS---SAGGLGLTICGE 422 P GK+ + + + + + D+G GI + E VF+ F N + GLGL I E Sbjct: 369 PEGGKVTVGLEKKRSQMIITVSDNGPGIPAEVRERVFERFYRNAAPDQPGSGLGLAIVRE 428 Query: 423 IVDSMQATISL 433 IV + Q +I L Sbjct: 429 IVQASQGSIRL 439 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 171 bits (432), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 127/381 (33%), Positives = 193/381 (50%), Gaps = 26/381 (6%) Query: 96 LPSYKGKADKEAIYPALAHIYEDTYNGVPVRVAAL--------FQPVVTNDPRGAALVQV 147 +P D+ + D +G VR+A L Q + +PR A VQV Sbjct: 1 MPQPPPDEDRSTV--GTVQFRNDILHGSDVRIATLQVDLRRSAAQRAASREPR-LATVQV 57 Query: 148 AETMENRSALARKIFWETLIRQAALLVVIVSVTLYVVRRALQPVDALRRQLDERPADDLS 207 ET+E R+ LA +I ++ Q +L V +++ + + R L P+ L++++ R DDLS Sbjct: 58 GETLEKRAQLANEIIKGVILPQFIILPVALALVWFALSRGLSPLSELQQRIRARRPDDLS 117 Query: 208 PVAPPMAPRELQPFIDALNQLMARLRRLLDHQQRFVANASHQLRTPLAVLKTQLQSGLRG 267 P+ P E+ P + +LN ++ARL + + Q+RF+A+A+HQ++TPLA ++ Q + LR Sbjct: 118 PIDSGQVPEEISPLVRSLNDMLARLSQTIAIQKRFIADAAHQMKTPLAGMRMQSELALRQ 177 Query: 268 DAPAPV--IWQEMSGTVERATTLANQLLSLAKVE-QIRGRGAQEVCDLGMLARETAVDLS 324 D V ++++ + E AT L NQLLSLA+ E Q G +L LAR D Sbjct: 178 DDQVDVRRSLEQLAKSSETATRLVNQLLSLARAENQQSQSGGMIPVELNELARSVVQDWV 237 Query: 325 PLIAEK--DLDFELEGEKILVMGHPWMIGELISNLLHNAIRHTPVHGKLGIRISARED-- 380 P+ + DL FE IL+ G+P M+ EL+SNLL NA+ +TP G + + AR D Sbjct: 238 PMSFTQRIDLGFEQPDHPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTVRARTDEG 297 Query: 381 --VAELLIWDSGEGIDDQAMENVFKAF-----SSNVSSAGGLGLTICGEIVDSMQATISL 433 +A L + D+G GI NVF+ F + + S GLGL I EI +A + + Sbjct: 298 GGLAILEVEDNGPGIPVAERANVFERFYRILGTPSTGSGLGLGLAIVREIAQQHEAALEI 357 Query: 434 -RNRIGKDGAVEGLDALVHFR 453 N D G V FR Sbjct: 358 TSNPRSTDPKYPGCVFRVSFR 378 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 168 bits (426), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 137/450 (30%), Positives = 212/450 (47%), Gaps = 17/450 (3%) Query: 1 VRAYLLAWIISPIALFIVIDSFSLYRNTLESVNTAYDRMLIASVHSIGDLLRIENGELKA 60 +R LL W+I+P+ L +I + Y A+D+ L+ S + +R A Sbjct: 8 IRRDLLKWLIAPLLLVNLIGAGLTYWLAWLPAQHAFDQNLMDSTWGLYAQVRHRQERTTA 67 Query: 61 SLPYAALEIYEADYSSRMIYRINGLDGKFFEGDQDLPSYKGKADKEAIYPALAHIYEDTY 120 L A +I +++S + + +G+ GD+ P ++ + Y+ Sbjct: 68 ELTQQAEQILRSNHSDTTFFAVRNTEGQIVVGDKGFPQLPASD----VFDRPIN-YDGEM 122 Query: 121 NGVPVRVAALFQPVVTNDPRGAALVQVAETMENRSALARKIFWETLIRQAALLVVIVSVT 180 G PVR+AA+ Q V N G V VAET+ R I L+ AL + V Sbjct: 123 RGEPVRIAAM-QVRVKN---GVIWVAVAETIRKRDRAHYTILLSFLVLDGALTFASIFVV 178 Query: 181 LYVVRRALQPVDALRRQLDERPADDLSPVAPPMAPRELQPFIDALNQLMARLRRLLDHQQ 240 L VRR L+P+ +L+R L++R L + AP ELQP I A+N+LMAR+ + QQ Sbjct: 179 LVAVRRGLRPLKSLQRNLEQRDHGKLGAIDGRGAPVELQPLIVAMNELMARINKGEQAQQ 238 Query: 241 RFVANASHQLRTPLAVLKTQLQSGLRGDAPAPVIWQE---MSGTVERATTLANQLLSLAK 297 F+A+ +HQLRTPL LK QL+ P + M+ +VER + QLL+LA+ Sbjct: 239 NFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQSRQLLALAR 298 Query: 298 VEQ--IRGRGAQEVCDLGMLARETAVDLSPLIAEKDLDFELEGEKILVMGHPWMIGELIS 355 E R +E+ L L E+ +K +D + + G +++ +LI Sbjct: 299 SEPGLFESRKLEELS-LDKLVEESVQHFIEEADKKRIDLGFDLRPARLRGDRFLLSDLID 357 Query: 356 NLLHNAIRHTPVHGKLGIRISAREDVAELLIWDSGEGIDDQAMENVFKAF-SSNVSSAG- 413 NL+ NA+R++P HG + +R +D L + DSG GI + E +F F +N AG Sbjct: 358 NLIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDRFYRANDKVAGS 417 Query: 414 GLGLTICGEIVDSMQATISLRNRIGKDGAV 443 GLGL I EI IS+ + G + Sbjct: 418 GLGLAIVREIAHDHGGVISVDCKEQGQGTI 447 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,367,312 Number of extensions: 56564 Number of successful extensions: 324 Number of sequences better than 1.0e-08: 25 Number of HSP's gapped: 297 Number of HSP's successfully gapped: 25 Length of query: 457 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 369 Effective length of database: 1,203,190 Effective search space: 443977110 Effective search space used: 443977110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)