BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0005.p (401 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspi... 785 0.0 gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspi... 173 2e-44 gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspi... 166 3e-42 gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspi... 120 1e-28 >gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 785 bits (2028), Expect = 0.0, Method: Compositional matrix adjust. Identities = 396/401 (98%), Positives = 398/401 (99%) Query: 1 MTTLNDIEAAALRLQGQILETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKMV 60 MTTLNDIEAAA RLQGQILETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNK+V Sbjct: 1 MTTLNDIEAAARRLQGQILETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKLV 60 Query: 61 DVAASEGGARGVVAMSAGNHAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVL 120 DVAAS GGARGVVAMSAGNHAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVL Sbjct: 61 DVAASGGGARGVVAMSAGNHAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVL 120 Query: 121 HGDTLEAARTHAYELAEKEGLTFVHPYDDEAIIAGQGTVALEMMKAVPDLDCLVVSIGGG 180 HGDTLEAARTHAYELAEKEGLTFVHPYDDEAIIAGQGTVALEMMKAVPDLDCLVVSIGGG Sbjct: 121 HGDTLEAARTHAYELAEKEGLTFVHPYDDEAIIAGQGTVALEMMKAVPDLDCLVVSIGGG 180 Query: 181 GLLAGMAVAARAIKPEIEIVGVQTRRFPTMFNAVKGTEHPVGASTIAEGIAVGTPGTLTR 240 GLLAGMAVAARAIKPEIEIVGVQTRRFPTM NAVKGTEHPVGASTIAEGIAVGTPGTLTR Sbjct: 181 GLLAGMAVAARAIKPEIEIVGVQTRRFPTMVNAVKGTEHPVGASTIAEGIAVGTPGTLTR 240 Query: 241 AIIAREADELLLVDEGDIEQAVLMLLEIEKTLVEGAGAAGLAALVRYPDRFRGKRVGLVL 300 AIIAREADELLLVDEGDIEQAVLMLLEIEKTLVEGAGAAGLAALVRYPDRFRGKRVGLVL Sbjct: 241 AIIAREADELLLVDEGDIEQAVLMLLEIEKTLVEGAGAAGLAALVRYPDRFRGKRVGLVL 300 Query: 301 SGGNIDPLLLTAIIERGMVRAGRLARVKVSSRDVPGTLARITGIVAEAGANIDEVHHQRA 360 SGGNIDPLLLTAIIERGMVRAGRLARVKVSSRDVPGTLARITGIVAEAGAN+DEVHHQRA Sbjct: 301 SGGNIDPLLLTAIIERGMVRAGRLARVKVSSRDVPGTLARITGIVAEAGANVDEVHHQRA 360 Query: 361 FTLLAAQNVDIELVLQTRGRPHLAQVLQALRAAGFEAEELH 401 FTLLAAQNVDIELVLQTRGRPHLAQVLQALRAAGFEAEELH Sbjct: 361 FTLLAAQNVDIELVLQTRGRPHLAQVLQALRAAGFEAEELH 401 >gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 173 bits (439), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 114/279 (40%), Positives = 165/279 (59%), Gaps = 4/279 (1%) Query: 20 ETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKMVDVAASEGGARGVVAMSAGN 79 ETP + TLSQ + +++ K E++Q SFK RGA NKM + ++ RGV+ SAGN Sbjct: 20 ETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMAHLTPAQL-KRGVICASAGN 78 Query: 80 HAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVLHGDTLEAARTHAYELAEKE 139 HAQGVA A RLG A IVMP TP VKI+ + G VVL GD+ A HA L +K+ Sbjct: 79 HAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHALTLEKKQ 138 Query: 140 GLTFVHPYDDEAIIAGQGTVALEMMKAVPD-LDCLVVSIGGGGLLAGMAVAARAIKPEIE 198 LTFVHP+DD +IAGQGTV +E+++ PD + + V+IGGGGL+AG+A +A++P+I+ Sbjct: 139 KLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGGGLIAGVASYVKAVRPDIK 198 Query: 199 IVGVQTRRFPTMFNAVKGTEHPV--GASTIAEGIAVGTPGTLTRAIIAREADELLLVDEG 256 I+GVQT M ++K ++G AV G T I DE+++VD Sbjct: 199 IIGVQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDEVIIVDTD 258 Query: 257 DIEQAVLMLLEIEKTLVEGAGAAGLAALVRYPDRFRGKR 295 + A+ + + +++VE AGA +A Y +R + + Sbjct: 259 AVCTAIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASK 297 >gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 166 bits (420), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 116/308 (37%), Positives = 170/308 (55%), Gaps = 3/308 (0%) Query: 1 MTTLNDIEAAALRLQGQILETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKMV 60 + + D+ AA R++ TP + SRT +GAQ+F K EN Q +FK RGA N + Sbjct: 10 LPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNALT 69 Query: 61 DVAASEGGARGVVAMSAGNHAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVL 120 A++ GV+ S+GNHAQG A AQ LG+PA IVMP+ P K+ TRG+GA VV Sbjct: 70 QFDATQR-KHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVT 128 Query: 121 HGDTLEAARTHAYELAEKEGLTFVHPYDDEAIIAGQGTVALEMMKAVPDLDCLVVSIGGG 180 + E LA + +T + PYD ++AGQGT LE+++ LD L V +GGG Sbjct: 129 YDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDALFVCLGGG 188 Query: 181 GLLAGMAVAARAIKPEIEIVGVQTRRFPTMFNAVKGTE--HPVGASTIAEGIAVGTPGTL 238 GLL+G A+AARA+ P +I GV+ + +++ + H TIA+G G Sbjct: 189 GLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQTQHLGKY 248 Query: 239 TRAIIAREADELLLVDEGDIEQAVLMLLEIEKTLVEGAGAAGLAALVRYPDRFRGKRVGL 298 T +I D++ V + ++ + E K +VE G G AA + D+ +GKRVG+ Sbjct: 249 TFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQLQGKRVGV 308 Query: 299 VLSGGNID 306 ++SGGN+D Sbjct: 309 IISGGNVD 316 >gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 120 bits (302), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 100/309 (32%), Positives = 161/309 (52%), Gaps = 5/309 (1%) Query: 1 MTTLNDIEAAALRLQGQILETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKMV 60 + +L DIE AA + + T + L+Q +GA+V++K EN T +FK RG + Sbjct: 9 LPSLTDIEQAAGIVYAGMAPTAQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVYLH 68 Query: 61 DVAASEGGARGVVAMSAGNHAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVL 120 +A + GVV+ + GNH Q V A+RLG+ ATIV+P+ K R G ++ Sbjct: 69 HLARQQPQLSGVVSATRGNHGQSVGLAARRLGISATIVVPQGNSREKNAAMRALGVELIE 128 Query: 121 HGDTLEAARTHAYELAEKEGLTFVHPYDDEAIIAGQGTVALEMMKAVPDLDCLVVSIGGG 180 HG + +R +A +LA ++ L + P ++AG + +E+ A P LD ++V IG G Sbjct: 129 HGSEFQESREYAQQLAAQQQLHMI-PSLHRDLVAGVASYWMELFAAQPGLDVVLVPIGQG 187 Query: 181 GLLAGMAVAARAIKPEIEIVGVQTRR---FPTMFNAVKGTEHPVGASTIAEGIAVGTPGT 237 + G A A+ I+GV + + F A + E PV ++ IA+G+A P Sbjct: 188 SGMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIESPV-STCIADGVACRVPDQ 246 Query: 238 LTRAIIAREADELLLVDEGDIEQAVLMLLEIEKTLVEGAGAAGLAALVRYPDRFRGKRVG 297 + ++ E DE++ V + ++ A+ + + EGAGA LAA + R RG +VG Sbjct: 247 ASLEVLFSEVDEVVAVTDDEVMDAMKLAYIATHNVAEGAGACALAAAWQLRARLRGLKVG 306 Query: 298 LVLSGGNID 306 + LSGGN+D Sbjct: 307 VTLSGGNVD 315 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,123,630 Number of extensions: 44672 Number of successful extensions: 203 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 196 Number of HSP's successfully gapped: 4 Length of query: 401 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 314 Effective length of database: 1,207,925 Effective search space: 379288450 Effective search space used: 379288450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)