BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0204.p (774 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310478|ref|YP_003774570.1| NADP-dependent malic enzyme oxi... 1504 0.0 gi|300312657|ref|YP_003776749.1| NADP-dependent malic enzyme oxi... 879 0.0 >gi|300310478|ref|YP_003774570.1| NADP-dependent malic enzyme oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 758 Score = 1504 bits (3894), Expect = 0.0, Method: Compositional matrix adjust. Identities = 745/758 (98%), Positives = 751/758 (99%) Query: 17 MTQQISAAEQLLRDAAREYHRTPSRGKIEVRPTKPLSNQRDLSLAYSPGVAYPCLDIEAD 76 MTQQISAAEQLLRDAAREYHRTPSRGKIEVRPTKPLSNQRDLSLAYSPGVAYPCLDIEAD Sbjct: 1 MTQQISAAEQLLRDAAREYHRTPSRGKIEVRPTKPLSNQRDLSLAYSPGVAYPCLDIEAD 60 Query: 77 PLKAFDYTSRGNLVAVISNGTAVLGLGNIGPLASKPVMEGKGCLFKKFAGVDVFDIELAE 136 PLKAFDYTSRGNLVAVISNGTAVLGLGNIGPLASKPVMEGKGCLFKKFAGVDVFDIELAE Sbjct: 61 PLKAFDYTSRGNLVAVISNGTAVLGLGNIGPLASKPVMEGKGCLFKKFAGVDVFDIELAE 120 Query: 137 NDPDKLVEIIAALEPTLGGINLEDIKAPECFYIEQKLSARMNIPVFHDDQHGTAIISSAA 196 NDPDKLVEIIA+LEPTLGGINLEDIKAPECFYIEQKLSARMNIPVFHDDQHGTAIISSAA Sbjct: 121 NDPDKLVEIIASLEPTLGGINLEDIKAPECFYIEQKLSARMNIPVFHDDQHGTAIISSAA 180 Query: 197 LLNGLELVGKDIGAVKIAVSGAGAAAIACVEVMIGLGVKRENVYMVDSKGVIYEGRPGGL 256 LLNGLELVGKDIG VKIAVSGAGAAAIACVEVMIGLGVKRENVYMVDSKGVIYEGRPGGL Sbjct: 181 LLNGLELVGKDIGKVKIAVSGAGAAAIACVEVMIGLGVKRENVYMVDSKGVIYEGRPGGL 240 Query: 257 DASKQRYAIKTEARTLADVVNGADVFLGCSAPGVLTAEMVKTMADKPIILALANPEPEIR 316 DASKQRYAIKTEARTLADVVNGADVFLGCSAPGVL+AEMVKTMADKPIILALANPEPEIR Sbjct: 241 DASKQRYAIKTEARTLADVVNGADVFLGCSAPGVLSAEMVKTMADKPIILALANPEPEIR 300 Query: 317 PELAKSVRPDCIIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKLACVREIADL 376 PELAKSVRPDCIIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKLACVREIADL Sbjct: 301 PELAKSVRPDCIIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKLACVREIADL 360 Query: 377 AKAETSAEVSAAYPGKELVFGADYLIPTPFDSRLILKIAPAVAKAAAESGVATRPIKDYA 436 AKAETSAEVSAAYPGKELVFGADYLIPTPFDSRLILKIAPAVAKAAAESGVATRPIKDY Sbjct: 361 AKAETSAEVSAAYPGKELVFGADYLIPTPFDSRLILKIAPAVAKAAAESGVATRPIKDYD 420 Query: 437 AYREELTRFIYQTGMFMQPVFNTAKVAPQRVVYADGQDERVLRAVQFVADEGLAAPILVG 496 AYR ELTRFIYQTGMFMQPVFNTAKVAPQRVVYADGQDERVLRAVQFVADEGLAAPILVG Sbjct: 421 AYRAELTRFIYQTGMFMQPVFNTAKVAPQRVVYADGQDERVLRAVQFVADEGLAAPILVG 480 Query: 497 QPAAIEAGIKKAGLRIKIGQNVQVIDPASDARVAQYAAQYKDGSEADVRASGSLLAALMV 556 PAAIEAGIKKAGLRIKIGQNVQVIDPA+DARVAQYAAQYKDG EADVRASGSLLAALMV Sbjct: 481 APAAIEAGIKKAGLRIKIGQNVQVIDPATDARVAQYAAQYKDGGEADVRASGSLLAALMV 540 Query: 557 KAGDADAMLCGLDGNYDGHLAHVEKVIGLKDGAQEFAALNAVLLPEQTLFITDTYVNDTP 616 KAGDADAMLCGLDGNYDGHLAHVEKVIGLK GAQEFAALNAVLLP+QTLFITDTYVNDTP Sbjct: 541 KAGDADAMLCGLDGNYDGHLAHVEKVIGLKQGAQEFAALNAVLLPDQTLFITDTYVNDTP 600 Query: 617 DAEQLARIAKAAAEEVARFGVPPKVAFLSHSNHGSSSRPSARKMAAAHEAFKKLAPDVES 676 DAEQLARIA+AAAEEVARFGVPPKVAFLSHSN GSSSRPSARKMAAAHEAFKKLAP+VES Sbjct: 601 DAEQLARIAQAAAEEVARFGVPPKVAFLSHSNRGSSSRPSARKMAAAHEAFKKLAPEVES 660 Query: 677 AGELHGDAALSEKIRNNAGLADSGLTGAANVLVCPNLDAANILFNVLKTSSGQGTTIGPI 736 AGELHGDAALSEKIRNNAGLADSGLTGAANVLVCPNLDAANILFNVLKTSSGQGTTIGPI Sbjct: 661 AGELHGDAALSEKIRNNAGLADSGLTGAANVLVCPNLDAANILFNVLKTSSGQGTTIGPI 720 Query: 737 LLGAAAPVHVLTPSSTVRRVINMTALAAAKAQDGKSKA 774 LLGAAAPVHVLTPSSTVRRVINMTALAAAKAQDGKSKA Sbjct: 721 LLGAAAPVHVLTPSSTVRRVINMTALAAAKAQDGKSKA 758 >gi|300312657|ref|YP_003776749.1| NADP-dependent malic enzyme oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 770 Score = 879 bits (2270), Expect = 0.0, Method: Compositional matrix adjust. Identities = 450/765 (58%), Positives = 567/765 (74%), Gaps = 14/765 (1%) Query: 16 TMTQQISAAEQLLRDAAREYHRTPSRGKIEVRPTKPLSNQRDLSLAYSPGVAYPCLDIEA 75 +M + Q LR AA +YH P+ GKI V PTK L+NQRDLSLAYSPGVA C +I A Sbjct: 3 SMISKKEEVRQQLRQAALDYHEFPTPGKIAVTPTKLLTNQRDLSLAYSPGVAAACEEIVA 62 Query: 76 DPLKAFDYTSRGNLVAVISNGTAVLGLGNIGPLASKPVMEGKGCLFKKFAGVDVFDIELA 135 DPL AF YT+RGNLVAVISNGTAVLGLGNIG LASKPVMEGKG LFKKFAG+DVFDIE+ Sbjct: 63 DPLNAFRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAGIDVFDIEVN 122 Query: 136 ENDPDKLVEIIAALEPTLGGINLEDIKAPECFYIEQKLSARMNIPVFHDDQHGTAIISSA 195 E DPDKL+++IAALEPT GGINLEDIKAPECFYIE+KL +M IPVFHDDQHGTAII A Sbjct: 123 ETDPDKLIDVIAALEPTFGGINLEDIKAPECFYIERKLREKMKIPVFHDDQHGTAIIVGA 182 Query: 196 ALLNGLELVGKDIGAVKIAVSGAGAAAIACVEVMIGLGVKRENVYMVDSKGVIYEGRPGG 255 A+LNGL++VGKDI VK+ VSGAGAAA+AC+++++ LG EN+Y+ D GV+Y+GR Sbjct: 183 AILNGLKVVGKDIKNVKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGRKEL 242 Query: 256 LDASKQRYAIKTEARTLADVVNGADVFLGCSAPGVLTAEMVKTMADKPIILALANPEPEI 315 +D K+R+A +TEARTLA+V+ AD+FLG SA GVL EMVK MA +P++LALANP PEI Sbjct: 243 MDPDKERFARETEARTLAEVIPDADIFLGLSAAGVLKPEMVKQMAARPLVLALANPTPEI 302 Query: 316 RPELAKSVRPDCIIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKLACVREIAD 375 PE K+VR D IIATGRSDYPNQVNNVLCFPYIFRGALD GAT IT M++A V IA+ Sbjct: 303 LPEEVKAVRDDAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIATVHAIAE 362 Query: 376 LAKAETSAEVSAAYPGKELVFGADYLIPTPFDSRLILKIAPAVAKAAAESGVATRPIKDY 435 LA+AE S V+ Y L FG +Y+IP PFD RL++KIAPAVA+AA SGVA RPI+D Sbjct: 363 LAQAEQSDIVATTYGIANLSFGPEYIIPKPFDPRLMIKIAPAVARAAESSGVAARPIQDM 422 Query: 436 AAYREELTRFIYQTGMFMQPVFNT---AKVAPQRVVYADGQDERVLRAVQFVADEGLAAP 492 AY E+L +F+Y +G FM+P+F A A +R+VYA+G++ERVLRAVQ + DE LA P Sbjct: 423 DAYIEKLEQFVYHSGTFMRPIFQVAKKAAEAKKRIVYAEGEEERVLRAVQVIVDENLAKP 482 Query: 493 ILVGQPAAIEAGIKKAGLRIKIGQNVQVIDPASDARVAQYAAQYKDGS----------EA 542 ILVG+P +E I+K GLR++ G + +VI+P D R Y + + S + Sbjct: 483 ILVGRPEVLEQRIQKFGLRLRAGTDFEVINPNFDNRYRDYWQTFLEMSRRKGVTEQYAKL 542 Query: 543 DVRASGSLLAALMVKAGDADAMLCGLDGNYDGHLAHVEKVIGLKDGAQEFAALNAVLLPE 602 ++R +L+ ++ + GDAD M+CG G HL ++++V+G ++G +AA+NAV+LP Sbjct: 543 EMRRRHTLIGSMAIHKGDADGMICGTYGTTQLHLHYIDQVLGKREGVNVYAAMNAVILPN 602 Query: 603 QTLFITDTYVNDTPDAEQLARIAKAAAEEVARFGVPPKVAFLSHSNHGSSSRPSARKMAA 662 + L + DT+VN+ P+A +LA I AAEE+ RFG+ P+ A LSHSN GSS+ SARKM Sbjct: 603 RQLVMVDTHVNENPNARELAEITMLAAEEMRRFGLHPRAALLSHSNFGSSNSESARKMRE 662 Query: 663 AHEAFKKLAPDVESAGELHGDAALSEKIRNNAGLADSGLTGAANVLVCPNLDAANILFNV 722 A +++AP++E GE+HGD AL K+ NA + DS L G AN+LV PN+DAANI +N+ Sbjct: 663 ALAILREIAPELEVDGEMHGDTALDSKLL-NAVIPDSPLKGDANLLVMPNIDAANIAYNL 721 Query: 723 LKTSSGQGTTIGPILLGAAAPVHVLTPSSTVRRVINMTALAAAKA 767 LKT+SG G IGPILLG A PVH+LTPS+TVRR+INMTAL A Sbjct: 722 LKTASGNGVAIGPILLGCAKPVHILTPSATVRRIINMTALCVMDA 766 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,201,460 Number of extensions: 89626 Number of successful extensions: 318 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 315 Number of HSP's successfully gapped: 2 Length of query: 774 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 681 Effective length of database: 1,179,515 Effective search space: 803249715 Effective search space used: 803249715 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)