BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0202.p (439 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310476|ref|YP_003774568.1| major facilitator superfamily t... 756 0.0 gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 602 e-173 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 309 3e-85 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 303 2e-83 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 297 9e-82 >gi|300310476|ref|YP_003774568.1| major facilitator superfamily transporter protein [Herbaspirillum seropedicae SmR1] Length = 449 Score = 756 bits (1951), Expect = 0.0, Method: Compositional matrix adjust. Identities = 402/439 (91%), Positives = 419/439 (95%) Query: 1 VETIKPSASAFAAVEPSVLQHAVSKIKHRVLPLFVVMFIVNYIDRVNIGFVRSHLATDVG 60 VET +P+ASA A P+VLQ AV+KIK RVLPLFV+MFIVNYIDRVNIGFVRSHLATDVG Sbjct: 8 VETPQPAASATGAAAPTVLQQAVAKIKQRVLPLFVIMFIVNYIDRVNIGFVRSHLATDVG 67 Query: 61 ISTAAYGLGAGLFFVGYAIFEVPSNLLLQRFGAKAWLTRIMATWGLAATAMAFVNSETTF 120 I TAAYGLGAGLFFVGYA+FEVPSNLLLQRFGAKAWLTRIMATWGLAATAMAFVNSETTF Sbjct: 68 IGTAAYGLGAGLFFVGYALFEVPSNLLLQRFGAKAWLTRIMATWGLAATAMAFVNSETTF 127 Query: 121 YLLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGSALASILSGPISGGLLQLE 180 Y+LRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGSALASILSGPISGGLLQ++ Sbjct: 128 YVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGSALASILSGPISGGLLQID 187 Query: 181 GAGLHGWQWMFIIEGMASVVLCGFVWFWLDSMPADARWLSAEERTAITGTIVAEQAERMK 240 G GL GWQWMFIIEGMASV+LCGFVWFWLDSMPADA+WL+A+ER+AIT IV EQAERMK Sbjct: 188 GGGLQGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLTAQERSAITRAIVDEQAERMK 247 Query: 241 HKPAHVSPLQLLRDPQILIFCFIYFSISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIP 300 H+PA VSP QLLRDPQILIFCFIYFSISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIP Sbjct: 248 HQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIP 307 Query: 301 WLISIVAMYAFAALAARFRHQQAWAATALVIAAIGMFASTFGNSVYAFVSICFAAVGFKA 360 WLISIVAMYAFAALAARF+HQQAWAATALVIAA GMFASTFGNSVYAFVSICFAAVGFKA Sbjct: 308 WLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFASTFGNSVYAFVSICFAAVGFKA 367 Query: 361 ASSLFWPLPQGYLDARIAAGVIALINSIGNLGGFVAPTTFGYLEQRTGSIQGGLMGLAIT 420 ASSLFWPLPQ YLDARIAAGVIALINSIGNLGGFVAPT FG+LEQRTGSIQGGLMGLAIT Sbjct: 368 ASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVAPTAFGFLEQRTGSIQGGLMGLAIT 427 Query: 421 SLVAAGVVFLARSGPGTQR 439 SLVAAGVVFLARSGPG R Sbjct: 428 SLVAAGVVFLARSGPGATR 446 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 602 bits (1551), Expect = e-173, Method: Compositional matrix adjust. Identities = 302/417 (72%), Positives = 349/417 (83%) Query: 19 LQHAVSKIKHRVLPLFVVMFIVNYIDRVNIGFVRSHLATDVGISTAAYGLGAGLFFVGYA 78 L AVSKIK R+LPLFV MFI+NYIDRVNIGFVRSHL+TD+GI AA+GLGAGLFFVGYA Sbjct: 14 LTSAVSKIKWRILPLFVAMFIINYIDRVNIGFVRSHLSTDLGIGAAAFGLGAGLFFVGYA 73 Query: 79 IFEVPSNLLLQRFGAKAWLTRIMATWGLAATAMAFVNSETTFYLLRFLLGAAEAGFFPGV 138 +FEVPSN+LLQR+GA+AWLTRIM TWGL A MAFV SET+FY +RFLLG AEAGFFPGV Sbjct: 74 LFEVPSNMLLQRYGARAWLTRIMFTWGLVAACMAFVQSETSFYFMRFLLGVAEAGFFPGV 133 Query: 139 IYYFTQWLPASERGRAMAIFLSGSALASILSGPISGGLLQLEGAGLHGWQWMFIIEGMAS 198 +YYFTQWLP ERGRAMA+FLSGSA+ASILSGPI+G LL +EG GLHGWQWMFIIEG AS Sbjct: 134 VYYFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGGGLHGWQWMFIIEGGAS 193 Query: 199 VVLCGFVWFWLDSMPADARWLSAEERTAITGTIVAEQAERMKHKPAHVSPLQLLRDPQIL 258 +V C VW LDS P +A+WLS E+R A+ I AEQ +R ++PAHVS L LLRDPQI+ Sbjct: 194 MVFCVVVWLVLDSTPREAKWLSQEQREALAAVIAAEQEQRAANRPAHVSALSLLRDPQIV 253 Query: 259 IFCFIYFSISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARF 318 +FCFIYF+I LTIYGATFWLP+II+KMG F+DFQVGLFNSIPW+++I AMY FA LAAR+ Sbjct: 254 LFCFIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAIAAMYLFAVLAARY 313 Query: 319 RHQQAWAATALVIAAIGMFASTFGNSVYAFVSICFAAVGFKAASSLFWPLPQGYLDARIA 378 + Q W AT LVI+A GM S V FV++CFA +GFKAASSLFWP+PQ YLDARIA Sbjct: 314 KKPQFWVATTLVISAAGMAISAVAGPVIGFVAVCFAGIGFKAASSLFWPIPQAYLDARIA 373 Query: 379 AGVIALINSIGNLGGFVAPTTFGYLEQRTGSIQGGLMGLAITSLVAAGVVFLARSGP 435 A V+ALINS+GNLGGF AP TFGYLEQ+TGSIQGGL L+ SL+AA +VFLAR+ P Sbjct: 374 AAVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLFALSGASLLAAVLVFLARTRP 430 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 309 bits (791), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 169/411 (41%), Positives = 247/411 (60%), Gaps = 10/411 (2%) Query: 24 SKIKHRVLPLFVVMFIVNYIDRVNIGFVRSHLATDVGISTAAYGLGAGLFFVGYAIFEVP 83 SK+ R+LPL + ++ +Y+DRVN+GF + + D+ S YGLGAG+FF+GY +FEVP Sbjct: 22 SKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFVFEVP 81 Query: 84 SNLLLQRFGAKAWLTRIMATWGLAATAMAFVNSETTFYLLRFLLGAAEAGFFPGVIYYFT 143 SN++L + GA+ W+ RIM TWG+ + AMA+V + FY++RFLLG AEAGFFPGVI Y T Sbjct: 82 SNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVILYLT 141 Query: 144 QWLPASERGRAMAIFLSGSALASILSGPISGGLLQ-LEGA-GLHGWQWMFIIEGMASVVL 201 W PA+ RG+ A+F++G AL+ ++ GP+SG ++ + G G GWQWMFI+E + S++L Sbjct: 142 YWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIPSLIL 201 Query: 202 CGFVWFWLDSMPADARWLSAEERTAITGTIVAEQAERMKHKPAHVSPLQLLRDPQILIFC 261 V ++ DA WLS EE+ + I E++++ H+S + + ++ + Sbjct: 202 GVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQK-----EHLSLGAMFANGRVWLGA 256 Query: 262 FIYFSISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARFRHQ 321 IYF + +YG +FWLP+II+ G VGL +IP+ + AM A R + Sbjct: 257 LIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRER 316 Query: 322 QAWAATALVIAAIGMFAST-FG-NSVYAFVSICFAAVGFKAASSLFWPLPQGYLDARIAA 379 + A I IG+ ST +G N+ A S+ A+VG LFW LP +L AA Sbjct: 317 RWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAA 376 Query: 380 GVIALINSIGNLGGFVAPTTFGYLEQRTGSIQGGLMGLAITSLVAAGVVFL 430 IALINS+GNL GFV+P G+L+ T S G+ LA SLV G++ L Sbjct: 377 AGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLA-ASLVIGGLLTL 426 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 303 bits (777), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 181/415 (43%), Positives = 256/415 (61%), Gaps = 10/415 (2%) Query: 19 LQHAVSKIKHRVLPLFVVMFIVNYIDRVNIGFVRSHLATDVGISTAAYGLGAGLFFVGYA 78 L+ KI R++P V +F++ +IDRVN+GF + + D+ S A YGLGAG+FF+GY Sbjct: 21 LEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYF 80 Query: 79 IFEVPSNLLLQRFGAKAWLTRIMATWGLAATAMAFVNSETTFYLLRFLLGAAEAGFFPGV 138 IFEVPSNLLL++ GA+ L RI WGLA+ AMA+V + T+FY+LRFLLG EAGFFPGV Sbjct: 81 IFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGV 140 Query: 139 IYYFTQWLPASERGRAMAIFLSGSALASILSGPISGGLLQ-LEGAG-LHGWQWMFIIEGM 196 + Y T W PA R R +F++ A+A + GPI+G ++ +EG G L WQW+FI+EG+ Sbjct: 141 VLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGI 200 Query: 197 ASVVLCGF-VWFWLDSMPADARWLSAEERTAITGTIVAEQAERMKHKPAHVSPLQLLRDP 255 S +L GF V WL PA+A+WLS E+ A+T + AE + K H S + Sbjct: 201 PS-ILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAPGK----HFSFKDACTNY 255 Query: 256 QILIFCFIYFSISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALA 315 ++ I +YF + FW PSII+++G + Q+GL ++IP++ +AM + Sbjct: 256 RVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHS 315 Query: 316 ARFRHQQAWAATALVIAAIGMFAS--TFGNSVYAFVSICFAAVGFKAASSLFWPLPQGYL 373 + ++ +A A +IA IG+ A+ T GN+ A S+ AA+G AA +FW +P +L Sbjct: 316 DKTGERRLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFL 375 Query: 374 DARIAAGVIALINSIGNLGGFVAPTTFGYLEQRTGSIQGGLMGLAITSLVAAGVV 428 AAG IALINSIGNL GFVAP G L+ TGS+ GL +A LVA+ +V Sbjct: 376 AGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLV 430 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 297 bits (761), Expect = 9e-82, Method: Compositional matrix adjust. Identities = 152/416 (36%), Positives = 245/416 (58%), Gaps = 10/416 (2%) Query: 24 SKIKHRVLPLFVVMFIVNYIDRVNIGFVRSHLATDVGISTAAYGLGAGLFFVGYAIFEVP 83 K+ R++P ++ +++ Y+DRVN+GF + ++ D+G S +GLGAGLFF+GY +FEVP Sbjct: 23 KKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFLFEVP 82 Query: 84 SNLLLQRFGAKAWLTRIMATWGLAATAMAFVNSETTFYLLRFLLGAAEAGFFPGVIYYFT 143 SN+L+ + GA+ W+ RIM TWG+ + A FV + T FY+LRFLLG AEAGF+PG+I Y T Sbjct: 83 SNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGIILYLT 142 Query: 144 QWLPASERGRAMAIFLSGSALASILSGPISGGLLQL--EGAGLHGWQWMFIIEGMASVVL 201 W P+ R + +A+F+SG +A IL P+SG ++ + GL GWQWMF+IE + +V++ Sbjct: 143 YWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAIPAVLI 202 Query: 202 CGFVWFWLDSMPADARWLSAEERTAITGTIVAE-QAERMKHKPAHVSPLQLLRDPQILIF 260 +LD+ A+WL+ EE+ ++ I + + + KH + ++D ++ + Sbjct: 203 GVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAI-----VKDARVWLM 257 Query: 261 CFIYFSISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARFRH 320 C IYFS + YG T W+P++++ G + ++GL ++IP+ +I+AM A R R Sbjct: 258 CLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRE 317 Query: 321 QQAWAATALVIAAIGMFASTF--GNSVYAFVSICFAAVGFKAASSLFWPLPQGYLDARIA 378 ++ ++ +G + N+ + S+ AA G + LFW LP +L A Sbjct: 318 RRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAA 377 Query: 379 AGVIALINSIGNLGGFVAPTTFGYLEQRTGSIQGGLMGLAITSLVAAGVVFLARSG 434 A IA INS+GNL GFV+P GYL+ T + G+ LA+ +V + + G Sbjct: 378 AVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSIATLATKPG 433 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.139 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,295,359 Number of extensions: 51894 Number of successful extensions: 359 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 316 Number of HSP's successfully gapped: 22 Length of query: 439 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 351 Effective length of database: 1,203,190 Effective search space: 422319690 Effective search space used: 422319690 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)