BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0201.p (424 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lac... 851 0.0 gi|300310489|ref|YP_003774581.1| glucarate dehydratase [Herbaspi... 216 3e-57 gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbas... 72 7e-14 gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbas... 70 3e-13 gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cyc... 65 1e-11 >gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lactonizing [Herbaspirillum seropedicae SmR1] Length = 424 Score = 851 bits (2198), Expect = 0.0, Method: Compositional matrix adjust. Identities = 408/424 (96%), Positives = 420/424 (99%) Query: 1 MKIARVTVTPIAFKDGPLLNASGIHEPYALRSIIEIETDNGYIGLGESYGDAPALKILDL 60 MKI RVTVTPIAFKDGPLLNASGIHEPYALRSIIEIETDNGYIGLGESYGDAPALKILDL Sbjct: 1 MKITRVTVTPIAFKDGPLLNASGIHEPYALRSIIEIETDNGYIGLGESYGDAPALKILDL 60 Query: 61 VKEQLIGLDPFNLNGLRAIVRGVVAAMAPASNAGAELAPGSHASKAVSNAYSAFEVACLD 120 VKE+L+GLDPFNLNGLRAIVRGVVAAMAPASNAGAELAPGSHASKAVSNAYSAFEVACLD Sbjct: 61 VKERLVGLDPFNLNGLRAIVRGVVAAMAPASNAGAELAPGSHASKAVSNAYSAFEVACLD 120 Query: 121 AQARYLNVPLVDLLGGAVRKEIPFSAYLFFKYAQHIDSPYAPDGWGEALSEEQIVAQARK 180 AQARYLNVPLVDLLGGAVRKEIPFSAYLFFKYAQHIDSPYAPD WGEALSEEQIVAQARK Sbjct: 121 AQARYLNVPLVDLLGGAVRKEIPFSAYLFFKYAQHIDSPYAPDAWGEALSEEQIVAQARK 180 Query: 181 MIEENGFKSIKLKAGALDPEHEVACIKALKKAFPDAPLRIDPNGNWSLETAIRMADLLGD 240 MIEENGF+SIKLKAGAL PEHEVACIKAL+KAFPDAPLRIDPNGNWSL+TAIRMA+LLGD Sbjct: 181 MIEENGFQSIKLKAGALAPEHEVACIKALRKAFPDAPLRIDPNGNWSLQTAIRMAELLGD 240 Query: 241 DLQYYEDPTPDLEGMAELHRRTGLPLATNMVVTDFDELRRSVALNSVQIVLADHHYWGGL 300 DLQYYEDPTP LEGMAELHRRTGLPLATNMVVTDFDELRRSVAL+SVQIVLADHHYWGGL Sbjct: 241 DLQYYEDPTPGLEGMAELHRRTGLPLATNMVVTDFDELRRSVALDSVQIVLADHHYWGGL 300 Query: 301 RDTQQLAKMCDIFGLGVSMHSNSHLGISLMAMSHVAAAVENLSYACDTHYPWQEPDEEVI 360 RDTQQLAKMCD+FGLGVSMHSNSHLGISLMAMSHVAAAVENLSYACDTHYPWQEPDEEVI Sbjct: 301 RDTQQLAKMCDVFGLGVSMHSNSHLGISLMAMSHVAAAVENLSYACDTHYPWQEPDEEVI 360 Query: 361 RGGKLPIRNGCVQITDAPGLGVEVDQEQLRKLHQLYLECGIRQRDDVGQMRKYKPEWKTV 420 +GGKLPIRNGCVQITDAPGLGVEVDQ+QLRKLHQLYLECGIRQRDDVGQMRKY+P+WKTV Sbjct: 361 QGGKLPIRNGCVQITDAPGLGVEVDQDQLRKLHQLYLECGIRQRDDVGQMRKYQPDWKTV 420 Query: 421 KPRF 424 KPRF Sbjct: 421 KPRF 424 >gi|300310489|ref|YP_003774581.1| glucarate dehydratase [Herbaspirillum seropedicae SmR1] Length = 455 Score = 216 bits (550), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 156/435 (35%), Positives = 220/435 (50%), Gaps = 26/435 (5%) Query: 2 KIARVTVTPIAFKDGPLLNASGIHEPYALRSIIEIETDNGYIGLGESYGDAPALKILDLV 61 ++ + V P+A D LLN SG H PY R+++ + G G+GE G + L+ Sbjct: 19 RVTEMRVIPVAGHDSMLLNLSGAHGPYFTRNLVILTDSAGNTGVGEVPGGEGIRQTLEDA 78 Query: 62 KEQLIGLDPFNLNGLRAIVRGVVAAMAPASNAGAELAPGSHASKAVSNAYSAFEVACLDA 121 + ++G N+ G+ R A A G L + + +A +A E A LD Sbjct: 79 RTLVVGQSIGNVQGILNRAR---TAFADRDTGGRGLQ--TFDLRIAIHAVTALEAALLDL 133 Query: 122 QARYLNVPLVDLLG-GAVRKEIPFSAYLFFKYAQH-IDSPY-----APDGWGE-----AL 169 ++L+VP+ LLG G R E+ YLF+ ++ D PY A D W AL Sbjct: 134 LGKFLDVPVAALLGEGQQRDEVEMLGYLFYVADRNKTDLPYLAAPDAQDDWTRLRHEAAL 193 Query: 170 SEEQIVAQARKMIEENGFKSIKLKAGALDPEHEVACIKALKKAFPDAPLRIDPNGNWSLE 229 + E +V A GF KLK G + E E+A + AL + FP A + +DPNG W L+ Sbjct: 194 TPEAVVRLAEAAYARYGFNDFKLKGGVMRGEEEIAAVTALAERFPKARITLDPNGGWLLK 253 Query: 230 TAIRMADLLGDDLQYYEDPTPDLEG------MAELHRRTGLPLATNMVVTDFDELRRSVA 283 AIR+ D L Y EDP +G MAE R TGL ATNMV TD+ ++ +++ Sbjct: 254 DAIRLCRDQHDVLAYAEDPCGAEDGYSGREVMAEFRRATGLQTATNMVATDWRQMGHAIS 313 Query: 284 LNSVQIVLADHHYWGGLRDTQQLAKMCDIFGLGVSMHSNSHLGISLMAMSHVAAAVENLS 343 L SV I LAD H+W ++ + ++A+MC +GL HSN+H +SL +HVAAA Sbjct: 314 LQSVDIPLADPHFW-TMQGSVRVAQMCHEWGLTWGSHSNNHFDVSLAMFTHVAAAAPGKI 372 Query: 344 YACDTHYPWQEPDEEVIRGGKLPIRNGCVQITDAPGLGVEVDQEQLRKLHQLYLECGIRQ 403 A DTH+ WQ D + + L I G VQ+ PGLGVE+D Q+ HQ Y G+ Sbjct: 373 TAIDTHWIWQ--DGQRLTREPLQIVGGKVQVPTKPGLGVELDMAQVEAAHQTYRNMGLGA 430 Query: 404 RDDVGQMRKYKPEWK 418 RDD M+ P WK Sbjct: 431 RDDAVAMQYLIPGWK 445 >gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 398 Score = 72.4 bits (176), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 74/288 (25%), Positives = 121/288 (42%), Gaps = 24/288 (8%) Query: 110 AYSAFEVACLDAQARYLNVPLVDLLGGAVRKEIPFSAYLFF-KYAQHIDSPYAPDGWGEA 168 A A E A DA + +P+ LLGG R+ +P + L A+ ID Sbjct: 114 AKCAIETALFDALGQRTGLPVSQLLGGRSRESLPIAWTLASGDTAKDIDEA--------- 164 Query: 169 LSEEQIVAQARKMIEENGFKSIKLKAGALDPEHEVACIKALKKAFPD-APLRIDPNGNWS 227 E+++AQ R I KLK G + + +VA + A+KKA D +R+D N WS Sbjct: 165 ---ERMLAQRRHNI-------FKLKIGRREVDADVAHVAAIKKALGDRGAVRVDVNMAWS 214 Query: 228 -LETAIRMADLLGDDLQYYEDPTPDLEGMAELHRRTGLPLATNMVVTDFDELRRSVALNS 286 LE +A L+ + E P + +A L R + + + +T + Sbjct: 215 ELEAQRGLAGLVDAGCELVEQPVASPQALARLKGRHAIAIMADESLTGPASAFALARIGG 274 Query: 287 VQIVLADHHYWGGLRDTQQLAKMCDIFGLGVSMHSNSHLGISLMAMSHVAAAVENLSYAC 346 + GGLR QQ+A + D G+ + + I +A +H A L + Sbjct: 275 ADVFAIKIEQSGGLRAAQQVAAIGDAAGIELYGGTMLEGAIGTIASAHAFATFRELQWGT 334 Query: 347 DTHYPWQEPDEEVIRGGKLPIRNGCVQITDAPGLGVEVDQEQLRKLHQ 394 + P +E I L ++ + I PGLG+ +D+E+L+ + Sbjct: 335 ELFGPLLLTEE--ILAEPLQYQDFQLAIPHRPGLGITLDEERLQYFRR 380 >gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 70.1 bits (170), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 97/400 (24%), Positives = 175/400 (43%), Gaps = 57/400 (14%) Query: 33 IIEIETDNGYIGLGESYGDAPALKILDLVKE---QLIGLDPFNL-NGLRAIVRGVVAAMA 88 ++IETD G +G GE + A + V+E LIG DP N+ + + RG Sbjct: 17 FLKIETDEGVVGWGEPIVEGRAHSVAAAVEELSDYLIGKDPRNIEDHWTVLYRG------ 70 Query: 89 PASNAGAELAPGSHASKAVS-NAYSAFEVACLDAQARYLNVPLVDLLGGAVRKEIPFSAY 147 G + A+ +A + + A D + + L VP+ LLGGAVR I ++ Sbjct: 71 -----------GFYRGGAIHMSALAGIDQALWDIKGKDLGVPVHQLLGGAVRNSIRVYSW 119 Query: 148 LFFKYAQHIDSPYAPDGWGEALSEEQIVAQARKMIEENGFKSIKLKAGALDPEHEVACIK 207 + D P A + + VA+ ++ NG + ++ E +A ++ Sbjct: 120 IGG------DRPAD-----TAAAAKSAVARGFTAVKMNGTEELQFIDSHDKIELTLANVQ 168 Query: 208 ALKKAF-PDAPLRIDPNGNWSLETAIRM-ADLLGDDLQYYEDP--TPDLEGMAELHRRTG 263 A+++A P+ + +D +G A + +L L + E+P + + E + E+ T Sbjct: 169 AVREAVGPNVGIGVDFHGRVHKPMAKALIKELEPYKLMFIEEPVLSENAEALKEIAHLTS 228 Query: 264 LPLATNMVVTDFDELRRSVALNSVQIVLADHHYWGGLRDTQQLAKMCDIFGLGVSMHSNS 323 P+A + + +R ++ V I+ D + GG+ +T+++A M + + + +++H Sbjct: 229 TPIALGERLYSRWDFKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCP- 287 Query: 324 HLG-ISLMAMSHVAAAVENL---SYACDTHYPWQEPDEEVIRGGK-LPIRNGCVQITDAP 378 LG I+L + V A N + HY + IR +G V+I P Sbjct: 288 -LGPIALASCLQVDAGSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGP 346 Query: 379 GLGVEVDQEQLRKLHQLYLECGIRQRDDVGQMRKYKPEWK 418 GLG+E+++E +RQR +VG R P W+ Sbjct: 347 GLGIEINEEY------------VRQRAEVGH-RWRNPIWR 373 >gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 64.7 bits (156), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 73/295 (24%), Positives = 126/295 (42%), Gaps = 36/295 (12%) Query: 110 AYSAFEVACLDAQARYLNVPLVDLLGGAVRKEIPFSAYLFF-KYAQHIDSPYAPDGWGEA 168 A A E A DA AR L VPL L GG VR +P + L A+ ID Sbjct: 108 AKCAIETALSDALARRLGVPLSTLFGGRVRDSLPIAWTLASGDTARDID----------- 156 Query: 169 LSEEQIVAQARKMIEENGFKSIKLKAGALDPEHEVACIKALKKAFPD-APLRIDPNGNWS 227 +A KM+ + KLK GA + + A + A+K+A + +R+D N W+ Sbjct: 157 --------EAEKMLAQRRHNLFKLKIGARTVQQDCAHVAAIKRALGERGSVRVDVNQAWT 208 Query: 228 LETAIRMADLLGD---DLQYYEDPTPDLEGMAELHRRTGLPLATNMVV---TDFDELRRS 281 A+ L D DL + +G+ L ++ + + + + D +L R+ Sbjct: 209 ETEAMLGMQWLADAGVDLVEQPIAASNHDGLRRLRQKNRIAIMADESLHGPQDAYQLARA 268 Query: 282 VALNSVQIVLADHHYWGGLRDTQQLAKMCDIFGLGVSMHSNSHL--GISLMAMSHVAAAV 339 A + + + + GGL QQ+A + V ++ + L I MA + + A Sbjct: 269 GAADVFAVKI---NQSGGLAGAQQVAAIAS--AAQVELYGGTMLEGAIGTMASAQLFATF 323 Query: 340 ENLSYACDTHYPWQEPDEEVIRGGKLPIRNGCVQITDAPGLGVEVDQEQLRKLHQ 394 L++ + P +E I L R+ +++ PGLG+ +D++++ L + Sbjct: 324 GTLAWGSELFGPLLLTEE--ILATPLRYRDFALELPTGPGLGITLDEDRVNALRR 376 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,397,683 Number of extensions: 60886 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 5 Length of query: 424 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 336 Effective length of database: 1,203,190 Effective search space: 404271840 Effective search space used: 404271840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)