BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0193.p (289 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 105 3e-24 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 102 3e-23 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 98 8e-22 gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspiri... 92 5e-20 gi|300313293|ref|YP_003777385.1| enoyl-CoA hydratase/carnithine ... 90 3e-19 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 105 bits (263), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 59/159 (37%), Positives = 91/159 (57%), Gaps = 9/159 (5%) Query: 38 AVRRGLMSAIEEG----EADAGVAAILVTGAGRNFIGGADI-----REFGKPPQSPLLTE 88 A+ GLM+ + E +A + I++TG+ + F GADI ++ + +T Sbjct: 27 ALSDGLMTELGEALLAFDAQEEIGCIIITGSEKAFAAGADISAMAGYDYMDVFKGEFITR 86 Query: 89 LCNRIEACNKPVLAAIHGAALGGGLEVALAAHYRVALSNARLGLPEVQLGLLPGAGGTQR 148 + KPV+AA+ G ALGGG E+A+ + +A NA+ G PE++LG++PGAGGTQR Sbjct: 87 NWETLRRIRKPVIAAVAGYALGGGCELAMMCDFIIAADNAKFGQPEIKLGIVPGAGGTQR 146 Query: 149 TPRLIGAQAALDMMLTGRHLSAKEALSLGLVDQLAEGDS 187 PR + A+D+ LTGR + A+EA GLV ++ D Sbjct: 147 LPRAVSKAKAMDLALTGRMMDAEEAERAGLVSRIVAADK 185 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 102 bits (255), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 65/176 (36%), Positives = 95/176 (53%), Gaps = 7/176 (3%) Query: 14 VHLAREGAILVVTIDNPPVNALGVAVRRGLMSAIEEGEADAGVAAILVTGAGRNFIG-GA 72 + L + G V+T+ NPP N A L ++E AD + ++VTG G F GA Sbjct: 7 LKLEKTGHTAVLTLSNPPANTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKFFSAGA 66 Query: 73 DIREFGKPPQSPL------LTELCNRIEACNKPVLAAIHGAALGGGLEVALAAHYRVALS 126 D++ F ++ E + A +AAI+G A+GGGLE ALA R+A Sbjct: 67 DLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACDIRIAEE 126 Query: 127 NARLGLPEVQLGLLPGAGGTQRTPRLIGAQAALDMMLTGRHLSAKEALSLGLVDQL 182 A++ LPE +GLLP AGGTQ P L+G A M+L G ++A+ AL +GLV+++ Sbjct: 127 QAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEEV 182 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 98.2 bits (243), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 65/179 (36%), Positives = 103/179 (57%), Gaps = 10/179 (5%) Query: 17 AREGA-ILVVTIDNPPV-NALGVAVRRGLMSAIEEGEADAGVAAILVTGAGRNFIGGADI 74 AR G +L +T++ P + NAL R +++A+ E D V ++++G + F GAD+ Sbjct: 13 ARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGNEKAFAAGADL 72 Query: 75 REFGKPPQSPLLTELCNR------IEACNKPVLAAIHGAALGGGLEVALAAHYRVALSNA 128 E + + T+L R I KP+LAA++G ALG G E+ + A +A A Sbjct: 73 NEM--IHKDAIATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHADIAIAARGA 130 Query: 129 RLGLPEVQLGLLPGAGGTQRTPRLIGAQAALDMMLTGRHLSAKEALSLGLVDQLAEGDS 187 ++G PE+ +G LPGAGGTQR R +G A+ M+L+G +SA +AL GLV ++ + D+ Sbjct: 131 KIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDA 189 >gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 92.0 bits (227), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 55/173 (31%), Positives = 89/173 (51%), Gaps = 4/173 (2%) Query: 14 VHLAREGAILVVTID-NPPVNALGVAVRRGLMSAIEEGEADAGVAAILVTGAGRNFIGGA 72 + L++ +L + D NA+ A+ + L A++E E+D+ V AIL TG + F G Sbjct: 5 IQLSKADGVLTIHFDREDKKNAITAAMYQQLADALKEAESDSAVRAILFTGKPKAFSAGN 64 Query: 73 DIREFGKPP---QSPLLTELCNRIEACNKPVLAAIHGAALGGGLEVALAAHYRVALSNAR 129 D+ +F K P Q + + +I +KP++AA+ G A+G G + L A NA+ Sbjct: 65 DLEDFLKNPPKTQDAPVFQFLWQISHASKPIVAAVSGVAVGIGTTLLLHCDLVYAADNAK 124 Query: 130 LGLPEVQLGLLPGAGGTQRTPRLIGAQAALDMMLTGRHLSAKEALSLGLVDQL 182 +P QLGL P A + P + G Q A + +L G A EA +G V+++ Sbjct: 125 FSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMGFVNRV 177 >gi|300313293|ref|YP_003777385.1| enoyl-CoA hydratase/carnithine racemase [Herbaspirillum seropedicae SmR1] Length = 259 Score = 89.7 bits (221), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 64/170 (37%), Positives = 86/170 (50%), Gaps = 9/170 (5%) Query: 16 LAREGAILVVTIDNPPV-NALGVAVRRGLMSAIEEGEADAGVAAILVTGAGRNFIGGADI 74 + + GAI +T++ NAL + R + E GV ++ G G +F G D+ Sbjct: 10 IQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFEN--LPEGVKVAILRGHGEHFCAGLDL 67 Query: 75 REFGKPPQSPLLTE------LCNRIEACNKPVLAAIHGAALGGGLEVALAAHYRVALSNA 128 E + + L +I PV+A +HGA +GGGLE+A AAH RVA + Sbjct: 68 SELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHIRVAEPST 127 Query: 129 RLGLPEVQLGLLPGAGGTQRTPRLIGAQAALDMMLTGRHLSAKEALSLGL 178 GLPE Q GL G GG+ R RLIG DMMLTGR LSA+E G+ Sbjct: 128 FYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGI 177 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 861,786 Number of extensions: 32581 Number of successful extensions: 157 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 153 Number of HSP's successfully gapped: 9 Length of query: 289 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 205 Effective length of database: 1,222,130 Effective search space: 250536650 Effective search space used: 250536650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)