BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0192.p (402 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase... 177 1e-45 gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase... 76 5e-15 gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase... 70 3e-13 >gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 512 Score = 177 bits (450), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 117/320 (36%), Positives = 163/320 (50%), Gaps = 14/320 (4%) Query: 1 VVGGGTMGAGIAVAVLDAGLPVTMIERDDEALARGRGHVEKVYDAQVAKGRLTQEARDAV 60 V+G GTMGAGIA AG PV + + + A + G + D V+K +LT A Sbjct: 18 VIGSGTMGAGIAQVAAVAGHPVLLYDSREGAAGKAIGAICTNLDKLVSKDKLTPALAQAA 77 Query: 61 LARLQGSTRYEDLSTADVVIEAVFEDLTVKQAVFAELDRVCKPGAVLATNTSYLDIDAIA 120 R+ + E+L AD+VIEA+ E L K +F +L+ + +LATNTS L I IA Sbjct: 78 SQRISVAQSLEELHQADLVIEAIVEQLAAKHELFGKLEAIVSTRCILATNTSSLSITQIA 137 Query: 121 ASISRPADVIGLHFFSPANVMKLLEIVVPRQVTGDVVATAFELAKRLRKVPVRAGVCDGF 180 A + P V+G+HFF+P +M L+EI+ + + A K PV A GF Sbjct: 138 AGLQHPERVVGMHFFNPVPMMALVEIISGLASDAALAQCLHDTALAWGKTPVMARSTPGF 197 Query: 181 IGNRIL-AVYRQAADALMEDGASPYEIDAAVRDF-GFAMGPYQVVDLAGGDIGWATRKR- 237 I NR+ Y + L E GA P IDA +R+ GF MGP++++DL G D+ +A + Sbjct: 198 IVNRLARPYYAEGLRMLHEGGAQPATIDAVMREAGGFRMGPFELMDLIGHDVNFAVTQSV 257 Query: 238 -RAATRDPRARYVHIADRLCERGWFGQKSGRGFYRYPDGPRSGAPDPEVLALIDEERARA 296 A DPR I L G+ G+KSGRGFYRY G +GAP PE + + + Sbjct: 258 FHAYFNDPRFTPSLIQQELVHAGYLGRKSGRGFYRY--GEAAGAPRPET----ERDYPKP 311 Query: 297 GITPRSFSK----EDIQRRY 312 TP+ + E I RY Sbjct: 312 TATPQLYGDHPLVEMIHTRY 331 >gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 805 Score = 75.9 bits (185), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 101/386 (26%), Positives = 168/386 (43%), Gaps = 55/386 (14%) Query: 1 VVGGGTMGAGIAVAVLDAGLPVTMIER------DDEALARGRGHVEKVYDAQVAKGRLTQ 54 V+G G MGA IA ++A +PV + + + + R +++K+ A + Sbjct: 21 VLGAGVMGAQIAAHCINARVPVVLFDLPAKEGPKNGIVQRAIENLKKLSPAPLGN----- 75 Query: 55 EARDAVLARLQGSTRYED----LSTADVVIEAVFEDLTVKQAVFAELDRVCKPGAVLATN 110 DA L ++ YED L D++IEA+ E + K ++ ++ P A+ A+N Sbjct: 76 -KDDAALIQV---ANYEDDLAVLEGCDLIIEAIAERMDWKHDLYKKVAPHIGPKAIFASN 131 Query: 111 TSYLDIDAIAASISR--PADVIGLHFFSPANVMKLLEIVVPRQVTGDVVATAFE--LAKR 166 TS L I+A++ A G+HFF+P M L+E+ +P Q T + E L Sbjct: 132 TSGLSINALSEGFDAGLKARFCGVHFFNPPRYMHLVEL-IPTQSTEPHILDQLEAFLTTT 190 Query: 167 LRKVPVRAGVCDGFIGNRILAVYRQAADALMEDGASPYEIDAAVRD------FGFAM-GP 219 L K VRA F+ NR+ V+ A + A Y + V D G A G Sbjct: 191 LGKGVVRAFDTPNFVANRV-GVFGILATIIE---AEKYGLSVDVVDDLTGAKLGRAKSGT 246 Query: 220 YQVVDLAGGD-IGWATRKRRAATRDPR--ARYVH--IADRLCERGWFGQKSGRGFYR--- 271 ++ D+ G D +G R + +D + Y + +L E+G GQKSG GFY+ Sbjct: 247 FRTADVVGLDTMGHVIRTMQDNLKDDPFFSSYATPPLLAKLVEQGALGQKSGAGFYKKVG 306 Query: 272 ---------YPDGPRSGAPDPEVLALIDEERARAGITPRSFSKEDIQRRYLAAMINEGAN 322 D GA +++A I +E+ + Q ++L A+ + + Sbjct: 307 KDILRIDPAKGDYVPGGAKADDIIARILKEKDPVKRMKALHDSTNPQGQFLWAIFRDAFH 366 Query: 323 --VVH-QRIALRPLDVDVTFLYGYGF 345 VH + +A D+D +G+G+ Sbjct: 367 YIAVHLESVADNARDLDFAMRWGFGW 392 >gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 323 Score = 69.7 bits (169), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 70/239 (29%), Positives = 114/239 (47%), Gaps = 13/239 (5%) Query: 1 VVGGGTMGAGIAVAVLDAGLPVTMIERDDEALARGRGHVEKVYDAQVAKGRLTQEARDAV 60 VVG G +GA A L GL V+ ++ +A + R + + + + A G A A Sbjct: 20 VVGTGVIGASWAAYFLAQGLEVSAVDPMPQAEQKLRAAIARHWPSLQALGL----AEHAS 75 Query: 61 LARLQGSTRYED-LSTADVVIEAVFEDLTVKQAVFAELDRVCKPGAVLATNTSYLDIDAI 119 RL+ + E L AD V E E +K +F +D VLA+++S L + + Sbjct: 76 QERLRFYDQLEAALEGADFVQENGPEREDLKIDLFRRMDAALPAQVVLASSSSGLLMSKV 135 Query: 120 AASISRPADVIGLHFFSPANVMKLLEIVVPRQVTGDVVATAFELAKRLRKVPVRA-GVCD 178 A+ P V+ H F+P +++ L+E++ Q + + V TA + + K P+ Sbjct: 136 QAACRHPQRVVLGHPFNPPHLIPLVEVIGGAQTSPEAVQTALDFYAAIGKKPIHVRKEVK 195 Query: 179 GFIGNRILAVYRQAADALMEDG-ASPYEIDAAVRDFG-----FAMGPYQVVDLAGGDIG 231 G I NR+ A + A L+E G AS +ID A+ +G MGPY + ++GG+ G Sbjct: 196 GHIANRLQAALWREAFHLVEQGVASTEDIDTAIA-YGPGLRWALMGPYLNLHMSGGEGG 253 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,383,660 Number of extensions: 64142 Number of successful extensions: 258 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 252 Number of HSP's successfully gapped: 3 Length of query: 402 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 315 Effective length of database: 1,207,925 Effective search space: 380496375 Effective search space used: 380496375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)