BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0191.p (371 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309598|ref|YP_003773690.1| acetyl-CoA acyltransferase [Her... 257 1e-69 gi|300309581|ref|YP_003773673.1| acetyl-CoA acetyltransferase [H... 207 1e-54 gi|300313421|ref|YP_003777513.1| 3-oxoadipyl-CoA thiolase [Herba... 187 2e-48 gi|300313287|ref|YP_003777379.1| beta-ketoadipyl-CoA thiolase [H... 182 4e-47 gi|300310259|ref|YP_003774351.1| acetyl-CoA acetyltransferase [H... 157 9e-40 >gi|300309598|ref|YP_003773690.1| acetyl-CoA acyltransferase [Herbaspirillum seropedicae SmR1] Length = 398 Score = 257 bits (656), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 157/365 (43%), Positives = 211/365 (57%), Gaps = 14/365 (3%) Query: 2 MREAVIVSTARTPLTKAHRGEFNVTPGPTLAAFAVRAAVERAR-IDPGQIEDAILGCGYP 60 +++A IV+ RTP+ KA RG F T L A+++AV + +DP IEDAI+GC +P Sbjct: 5 LQDAYIVAATRTPIGKAPRGMFKNTRPDDLLVRAIQSAVAQVPGLDPKLIEDAIVGCSFP 64 Query: 61 EGTTGRNIARQAIVRAGLPISIAGTTVNRFCASGLQAIAMAAGRIVVDGADAMIAGGVES 120 EG G N+AR A++ AGLP +I G T+NR+CASG+ AIAMAA RI V AD MIA G ES Sbjct: 65 EGAQGLNMARNAVLLAGLPNTIGGVTINRYCASGITAIAMAADRIRVGEADVMIAAGAES 124 Query: 121 ISQIRSRSATDSGDSGMDPWI-----VEHKPDLYLPMIETADIVATRYGISREDQDRFSE 175 +S + G P I + + M TA+ VA ++ +SRE QD FS Sbjct: 125 MSMVPMM--------GFHPSININAFKDENVGMAYGMGLTAEKVAQQWKVSREAQDEFSL 176 Query: 176 ASQRKTAEAQEAGRYRDEIVACTTTMQVTDKASGQTSLREVTVEADNCNRPGTTYEALAK 235 AS +K AQEAG + DE+ + + + A+G+ ++ TV D R + ALAK Sbjct: 177 ASHQKAIAAQEAGEFADEMTSFEIVERFPNLATGEIDVKTRTVSLDEGPRADSNLAALAK 236 Query: 236 LAPVMGADKFITAGNSSQLSDGASACVMMEARAAERAGLPVLGAFRGLAVAGCEPDEMGI 295 L PV A +TAGNSSQ SDGA A +++ + ++ L L F AV G P+ MGI Sbjct: 237 LKPVFAAKGSVTAGNSSQTSDGAGALIIVSEKILKQFNLTPLARFVSFAVRGVPPEIMGI 296 Query: 296 GPVFAVPRLLQRHGLRMEDIGLWELNEAFASQALYCQRRLGIPEELLNVNGGAISIGHPF 355 GP A+P L+ GL + I ELNEAFA+QAL LG+ +N GGAI++GHP Sbjct: 297 GPKEAIPAALKAGGLTQDQIDWIELNEAFAAQALAVIGDLGLDPSKVNPMGGAIALGHPL 356 Query: 356 GMTGA 360 G TGA Sbjct: 357 GATGA 361 >gi|300309581|ref|YP_003773673.1| acetyl-CoA acetyltransferase [Herbaspirillum seropedicae SmR1] Length = 391 Score = 207 bits (527), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 137/366 (37%), Positives = 203/366 (55%), Gaps = 20/366 (5%) Query: 2 MREAVIVSTARTPLTKAHRGEFNVTPGPTLAAFAVRAAVERARIDPGQIEDAILGCGYPE 61 M + VIV+ RT + K G + L A ++A + + I P I + ILG Sbjct: 1 MDDVVIVAAQRTAIGK-FGGALSKIAAADLGAQVIKALLAKTGIKPEAISEVILGQVLTA 59 Query: 62 GTTGRNIARQAIVRAGLPISIAGTTVNRFCASGLQAIAMAAGRIVVDGADAMIAGGVESI 121 G G+N ARQ+++++GLP + V + C SGL+A+ +AA I A +IAGG E++ Sbjct: 60 GL-GQNPARQSVIKSGLPDMVPAFVVGKVCGSGLKAVQLAAQAIRCGDAQIVIAGGQENM 118 Query: 122 SQI-----RSRSATDSGDSGM-DPWIVEHKPDLY--LPMIETADIVATRYGISREDQDRF 173 S SR GD+ + D IV+ D+Y M TA+ VA ++ ISRE+QD F Sbjct: 119 SASPHVLNNSRDGFRMGDAKLTDTMIVDGLWDVYNQYHMGITAENVAKKFEISREEQDAF 178 Query: 174 SEASQRKTAEAQEAGRYRDEIVACTTTMQVTDKASGQTSLREVTVEADNCNRPGTTYEAL 233 + ASQ K AQ+AG+++DEIV + K +T V + D + G T E+L Sbjct: 179 AAASQNKAEAAQKAGKFKDEIVP------IEIKGKKET----VVFDTDEFVKHGVTAESL 228 Query: 234 AKLAPVMGADKFITAGNSSQLSDGASACVMMEARAAERAGLPVLGAFRGLAVAGCEPDEM 293 A L P +TAGN+S ++DGA+A V+ A+ A GLPVL + + AG +P M Sbjct: 229 ATLRPAFDKAGTVTAGNASGINDGAAAVVVTSAKLAAELGLPVLAKIKAYSSAGLDPSIM 288 Query: 294 GIGPVFAVPRLLQRHGLRMEDIGLWELNEAFASQALYCQRRLGIPEELLNVNGGAISIGH 353 G+GPV A L++ G +D+ L E+NEAFA+QA+ +++G +NVNGGAI++GH Sbjct: 289 GMGPVPASQLTLKKAGWTPQDLDLMEINEAFAAQAIAVNKQMGWDTSKINVNGGAIALGH 348 Query: 354 PFGMTG 359 P G +G Sbjct: 349 PIGASG 354 >gi|300313421|ref|YP_003777513.1| 3-oxoadipyl-CoA thiolase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 187 bits (474), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 144/377 (38%), Positives = 201/377 (53%), Gaps = 29/377 (7%) Query: 4 EAVIVSTARTPLTKAHRGEFNVTPGPTLAAFAVRAAVERAR-IDPGQIEDAILGCGYPEG 62 EA+I RTP + + G LAA +R+ +ER +D ++ED + GC G Sbjct: 2 EALICDAIRTPFGR-YGGALGAVRADDLAAAPIRSLMERNPGVDWSRVEDILYGCANQAG 60 Query: 63 TTGRNIARQAIVRAGLPISIAGTTVNRFCASGLQAIAMAAGRIVVDGADAMIAGGVESIS 122 RN+AR A + AGLPI++ G+TVNR C S L A+ MAA I MIAGGVES++ Sbjct: 61 EDNRNVARMAGLLAGLPIAVPGSTVNRLCGSSLDAVGMAARAIKSGEVQLMIAGGVESMT 120 Query: 123 QI------------RSRSATDS--GDSGMDPWIVEHKPDLYLPMIETADIVATRYGISRE 168 + RS + D+ G ++P + +P ETA+ VAT + I+R Sbjct: 121 RAPFVMGKAESAFARSAAIFDTTIGWRFVNPLMKAQYGIDSMP--ETAENVATDFQINRA 178 Query: 169 DQDRFSEASQRKTAEAQEAGRYRDEIVACTTTMQVTDKASGQTSLREVTVEADNCNRPGT 228 DQD F+ SQ++ A AQ AG + EI T + D + V D RP T Sbjct: 179 DQDAFALRSQQRWAAAQAAGFFAGEIAPLTIPQKKGDP---------LVVTTDEHPRPDT 229 Query: 229 TYEALAKLAPVMGADKFITAGNSSQLSDGASACVMMEARAAERAGLPVLGAFRGLAVAGC 288 T LAKL V+ D +TAGN+S ++DGA A ++ +AA+ L G+A AG Sbjct: 230 TLATLAKLKGVVRPDGTVTAGNASGVNDGACALLLASPKAADLYRLKPRARVLGMATAGV 289 Query: 289 EPDEMGIGPVFAVPRLLQRHGLRMEDIGLWELNEAFASQALYCQRRLGIPEEL--LNVNG 346 P MG GP AV ++L + GL + + + ELNEAFA+Q L R LG+P++ +N NG Sbjct: 290 APRIMGFGPAPAVRKVLAQVGLTLAQMDVIELNEAFAAQGLAVMRDLGLPDDAAHVNPNG 349 Query: 347 GAISIGHPFGMTGAXSV 363 GAI+IGHP G +GA V Sbjct: 350 GAIAIGHPLGASGARLV 366 >gi|300313287|ref|YP_003777379.1| beta-ketoadipyl-CoA thiolase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 182 bits (462), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 136/377 (36%), Positives = 198/377 (52%), Gaps = 26/377 (6%) Query: 2 MREAVIVSTARTPLTKAHRGEFNVTPGPTLAAFAVRAAVER-ARIDPGQIEDAILGCGYP 60 M++ I RTP+ + + G L A +RA +ER ++D ++D I GC Sbjct: 1 MKDVFICDAIRTPIGR-YGGALKDVRADDLGAVPLRALMERNPQVDWKAVDDVIYGCANQ 59 Query: 61 EGTTGRNIARQAIVRAGLPISIAGTTVNRFCASGLQAIAMAAGRIVVDGADAMIAGGVES 120 G RN+AR +++ AGLP + G+T+NR C SG+ A+ AA I MIAGGVES Sbjct: 60 AGEDNRNVARMSLLLAGLPQEVPGSTINRLCGSGMDALGTAARAIKSGETQLMIAGGVES 119 Query: 121 ISQI-----RSRSATDSGDSGMDPWIVEHKPDLYLP-------MIETADIVATRYGISRE 168 +++ ++ SA + D I + + M ETA+ VA + +SR Sbjct: 120 MTRAPFVMGKADSAFSRQAAIQDTTIGWRFVNALMKQKYGVDAMPETAENVAVDFKVSRA 179 Query: 169 DQDRFSEASQRKTAEAQEAGRYRDEIVACTTTMQVTDKASGQTSLREVTVEADNCNRPGT 228 DQD+F+ SQ K A AQ G EIV + D +TV D R T Sbjct: 180 DQDQFAVRSQAKAAAAQANGTLAQEIVPVVIPQKKGDP---------ITVTQDEHPR-AT 229 Query: 229 TYEALAKLAPVMGADKFITAGNSSQLSDGASACVMMEARAAERAGLPVLGAFRGLAVAGC 288 + EALA+L V+ D +TAGN+S ++DGA A ++ A A E+ L G+A AG Sbjct: 230 SMEALARLKGVVKPDGSVTAGNASGVNDGACALLLASAEAVEKYQLKPRARILGMATAGV 289 Query: 289 EPDEMGIGPVFAVPRLLQRHGLRMEDIGLWELNEAFASQALYCQRRLGIPEE--LLNVNG 346 P MG+GP A ++L + G+ ++ + + ELNEAFASQ L R LG+ ++ +N NG Sbjct: 290 APRIMGMGPAPASKKVLAQLGMTIDQMDVIELNEAFASQGLAVLRELGVADDDARVNPNG 349 Query: 347 GAISIGHPFGMTGAXSV 363 GAI++GHP GM+GA V Sbjct: 350 GAIALGHPLGMSGARLV 366 >gi|300310259|ref|YP_003774351.1| acetyl-CoA acetyltransferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 157 bits (398), Expect = 9e-40, Method: Compositional matrix adjust. Identities = 121/370 (32%), Positives = 182/370 (49%), Gaps = 28/370 (7%) Query: 6 VIVSTARTPLTKAHRGEFNVTPGPTLAAFAVRAAVERARIDPGQI---EDAILGCGYPEG 62 VIVS ART + + +G P P L A A+ AA++RA++ QI E +GC G Sbjct: 7 VIVSAARTAM-GSFQGALADLPAPHLGAVAISAALQRAQLSAAQIAQIEQVWMGCVLQAG 65 Query: 63 TTGRNIARQAIVRAGLPISIAGTTVNRFCASGLQAIAMAAGRIVVDGADAMIAGGVESIS 122 G+ ARQA +RAGLP ++A T+N+ C S +QA+ + I+ A ++AGG+ES+S Sbjct: 66 L-GQAPARQAALRAGLPQTVACATLNKVCGSAMQAVMLGHDSILAGSASMVVAGGMESMS 124 Query: 123 QI-----RSRSATDSG-DSGMDPWIVEHKPDLYLP------MIETADIVATRYGISREDQ 170 ++R G D +D ++ D Y M A+ A+ +G +R Q Sbjct: 125 NAPYLLPKARRGYRLGHDRLLDHMFLDGLEDAYSDQYRGRLMGTFAEDCASEFGFTRAQQ 184 Query: 171 DRFSEASQRKTAEAQEAGRYRDEIVACTTTMQVTDKASGQTSLREVTVEADNCNRPGTTY 230 D F+ S + +A G + E+V D A + S V + D Sbjct: 185 DAFAVQSTLRARQAISGGGFDWEVVP-------VDVAGKKGS---VVISQDE-GPMAVNL 233 Query: 231 EALAKLAPVMGADKFITAGNSSQLSDGASACVMMEARAAERAGLPVLGAFRGLAVAGCEP 290 E +A L P D +TA NSS +SDGA+A ++M AA + GL L G A P Sbjct: 234 ERIATLKPAFSVDGTVTAANSSSISDGAAALLLMRESAALKQGLTPLARILGHASYAGAP 293 Query: 291 DEMGIGPVFAVPRLLQRHGLRMEDIGLWELNEAFASQALYCQRRLGIPEELLNVNGGAIS 350 ++ P+ + ++ + GL L+E+NEAFA + R L I E +NV+GGA + Sbjct: 294 EKFPTAPIGVLRKIFAKTGLDAASTDLFEINEAFAVVPMAASRELDIDMEKINVHGGACA 353 Query: 351 IGHPFGMTGA 360 +GHP G +GA Sbjct: 354 LGHPIGASGA 363 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,153,331 Number of extensions: 48782 Number of successful extensions: 199 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 185 Number of HSP's successfully gapped: 6 Length of query: 371 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 284 Effective length of database: 1,207,925 Effective search space: 343050700 Effective search space used: 343050700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)