BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0190.p (398 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 125 6e-30 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 116 3e-27 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 104 1e-23 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 96 4e-21 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 74 2e-14 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 125 bits (314), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 97/318 (30%), Positives = 157/318 (49%), Gaps = 15/318 (4%) Query: 12 FRDSVRAFLAEKLPKALSDKVASGKHLSRDEMEQWHAILNERGWLANHWPKEYGGTGWTV 71 RD+VR L + P K+ +R EQ+ L + GWLA P+EYGG+G + Sbjct: 13 LRDAVRD-LCKTFPPEYWRKIDE----ARGYPEQFVDALTKAGWLAALIPQEYGGSGLGL 67 Query: 72 IEKFIFEHECCLAHAPRVVPFGVNMLGPVLIKYGNEAQKQYWLPRILNGE-DWWCQGYSE 130 E + E G L+++G+ QK+ +LPRI +GE +E Sbjct: 68 TEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGSPEQKERYLPRIASGELRLQSMAVTE 127 Query: 131 PGAGSDLAAVKTHAVRDGDHYIVNGQKTWTTLGHYANMIFCLVRTN---REAKKQEGISF 187 P G+D +KT AV+ GD Y++NGQK W + +++++ L RT + ++K EG+S Sbjct: 128 PTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVSRKSEGMSI 187 Query: 188 LLIDMNAP---GVEVRPIITLDGEHEVNEVFFTDVRVPVQNLVGEEDRGWSCAKYLLTYE 244 L+D+ G+EVRPI+ + HE NE+FF ++ +P +NL+GEE RG+ L E Sbjct: 188 FLVDLKEAIGHGMEVRPILNMVN-HETNELFFDNLEIPAENLIGEEGRGFRYILDGLNAE 246 Query: 245 RTNIAG--IGFSVAALQRLKKIAATQRSHGRPLADDPLFAARMARVEIDLENMKTTNLRV 302 RT IA IG + + + A + RP+ + +A I++E + Sbjct: 247 RTLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEVEAANLMRYQA 306 Query: 303 ISAVAGGGAPGAESSMLK 320 + + GA+++M K Sbjct: 307 CALFDAQQSCGAQANMAK 324 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 116 bits (291), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 106/399 (26%), Positives = 171/399 (42%), Gaps = 34/399 (8%) Query: 5 FTPAEEAFRDSVRAFLAEKLPKALSDKVASGKHLSRDEMEQWHAI--LNERGWLANHWPK 62 +P E RD++R F E+L +D R+ A+ L E G + P+ Sbjct: 3 LSPEHEMIRDAMRHFAQERLLPFAAD-------WDRNHTFPAEALKELAELGAMGMCVPE 55 Query: 63 EYGGTGWTVIEKFIFEHECCLAH--APRVVPFGVNMLGPVLIKYGNEAQKQYWLPRILNG 120 ++GG G + + E +V ++ + KYG+ QK+ WL + G Sbjct: 56 QWGGAGMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARG 115 Query: 121 EDWWCQGYSEPGAGSDLAAVKTHAVRDGDHYIVNGQKTWTTLGHYANMIFCLVRTNREAK 180 E C +EP GSD AA++T A RDGD +I+NG K + T G +A + T+R A Sbjct: 116 EMLGCFCLTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAG 175 Query: 181 KQEGISFLLIDMNAPGVEVRPIITLDGEH--EVNEVFFTDVRVPVQNLVGEEDRGWSCAK 238 K+ GIS L+ + PG V G+H + ++ + RVP L+G+E G+ A Sbjct: 176 KK-GISCFLVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIAL 234 Query: 239 YLLTYERTNIAG--IGFSVAALQRLKKIAATQRSHGRPLADDPLFAARMARVEIDLENMK 296 L R IA +G + AA + A + S G P+ + R+A + L+ + Sbjct: 235 SNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAAR 294 Query: 297 TTNLRVISAVAGGGAPGAESSMLKIRGTEIRQEITALTRRAMGPYARPYQWHSLEEGHDG 356 R G E+SM K+ +E ++I + Q H Sbjct: 295 LMVWRAAQLKDQGRPCLKEASMAKMFASEAAEKIA----------SDAIQIH-------- 336 Query: 357 GTLGPQAAAGAAAHYFNNRKLSIFGGSNEIQKNIISKMI 395 G +G + Y + R I+ G+N+IQ+ +I + I Sbjct: 337 GGVGYTSDFPVERIYRDVRICQIYEGANDIQRLVIGRAI 375 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 104 bits (260), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 83/294 (28%), Positives = 142/294 (48%), Gaps = 18/294 (6%) Query: 3 LNFTPAEE--AFRDSVRAFL-AEKLPKALSDKVASGKHLSRDEMEQWHAILNERGWLANH 59 L+F E+ A R++V AF +E P+A A + M+ W L + G L Sbjct: 7 LSFDHGEDIAALREAVAAFAHSEIAPRA-----AEIDRSDQFPMDLWKK-LGDLGVLGIT 60 Query: 60 WPKEYGGTGWTVIEKFIFEHECCLAHAPRVVPFGV--NMLGPVLIKYGNEAQKQYWLPRI 117 +EYGG G + I E A A + +G N+ + + GNE QK+ +LPR+ Sbjct: 61 VSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRL 120 Query: 118 LNGEDWWCQGYSEPGAGSDLAAVKTHAVRDGDHYIVNGQKTWTTLGHYANMIFCLVRTNR 177 ++G+ SEP AGSD+ ++K A + GD Y++NG K W T G A+++ +T+ Sbjct: 121 ISGDFIGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDL 180 Query: 178 EAKKQEGISFLLIDMNAPGVEVRPIIT---LDGEHEVNEVFFTDVRVPVQNLVGEEDRGW 234 EA + G++ L++ G V + + G H E+ F D VP +N++G RG Sbjct: 181 EAGAR-GMTAFLVEKGYKGFSVAQKLDKLGMRGSH-TGELVFQDCEVPEENVLGGVGRGV 238 Query: 235 SCAKYLLTYERTNIAG--IGFSVAALQRLKKIAATQRSHGRPLADDPLFAARMA 286 + L +ER+ ++G +G A + + ++ G+ + + L ++A Sbjct: 239 NVLMSGLDFERSVLSGGPLGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLA 292 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 96.3 bits (238), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 99/404 (24%), Positives = 177/404 (43%), Gaps = 35/404 (8%) Query: 1 MDLNFTPAEEAFRDSVRAFLAEKLPKALSDKVASGKHLSRDEMEQWHAILNERGWLANHW 60 MD + AF+ S R F A ++ + K H D + A E G+ + Sbjct: 1 MDFELNQDQLAFQQSARDFAAGEMAP-FAAKWDEEAHFPLDVI----AKAGELGFCGLYT 55 Query: 61 PKEYGGTGWTVIEKFIFEHECCLAHAPRVVPFGV-NMLGPVLIKYGNEAQKQYWLPRILN 119 P+ GG G + ++ + E A + NM+ ++ +G + K W ++ Sbjct: 56 PEADGGLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLAA 115 Query: 120 GEDWWCQGYSEPGAGSDLAAVKTHAVRDGDHYIVNGQKTWTTLGHYANMIFCLVRTNREA 179 G +EPG+GSD A++KT A + HY++NG K + + +++ + RT E Sbjct: 116 GRKIGSYCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGEG 175 Query: 180 KKQEGISFLLIDMNAPGVEVRPIITLDG--EHEVNEVFFTDVRVPVQNLVGEEDRGWSCA 237 + G+S +++ NAPGV + G + F +V+VP +L+G+E +G+ A Sbjct: 176 AR--GVSAIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFA 233 Query: 238 KYLLTYERTNIA--GIGFSVAALQRLKKIAATQRSHGRPLADDPLFAARMARVEIDLENM 295 L R NIA +G + AAL ++ GRP+AD ++A ++ +L Sbjct: 234 MKGLDGGRINIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAA 293 Query: 296 KTTNLRVISAVAGGGAPGAES--SMLKIRGTEIRQEITALTRRAMGPYARPYQWHSLEEG 353 + +R+ + G+P A + +M K T++ + + G Y ++ LE Sbjct: 294 RQM-VRLAATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREY-PLE-- 349 Query: 354 HDGGTLGPQAAAGAAAHYFNNRKL-SIFGGSNEIQKNIISKMIL 396 YF + ++ I G+NEI + IIS+ +L Sbjct: 350 ----------------RYFRDVRVHQILEGTNEIMRVIISRKLL 377 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 74.3 bits (181), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 66/291 (22%), Positives = 127/291 (43%), Gaps = 20/291 (6%) Query: 1 MDLNFTPAEEAFRDSVRAFLAEKLPKALSDKVASGKHLSR-DEMEQWHAILNERGWLANH 59 +D + +++A + RAF A+ L K K R ++ + + G++ Sbjct: 3 IDFQLSESQKALQAGARAFAADVLRKVGPTITPLVKPDERFYATRPFYQAMADAGFIKAL 62 Query: 60 WPKEYGGTGWTVIEKFIFEHECCLA--HAPRVVPFGVNMLGPVLIKYGNEAQKQYWLPRI 117 P+ +GGT + ++ + E + P + G + ++ +G QKQ +LP Sbjct: 63 LPESFGGTALSTLDFALAAEELTAVDVNVPSAL-LGTGLGLHAVVHHGTPEQKQRFLPDF 121 Query: 118 L-NGEDWWCQGYSEPGAGSDL------AAVKTHAVRDGDHYIVNGQKTWTT-----LGHY 165 + +G ++E G++ V+T A +GD +++NG K +TT G Sbjct: 122 IEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEWVINGAKHYTTNGTGWDGKG 181 Query: 166 ANMIFCLVRTNREAKKQEGISFLLIDMNAPGVEVRPIITLDGEHEV--NEVFFTDVRVPV 223 A++ + RT+ Q+ ++ +++ NAPG+E+ ++ G V F +VRVP Sbjct: 182 AHLYSVICRTDPALPPQQSLAVIMVPGNAPGIEIVGLLDTMGHRATISPRVHFNNVRVPA 241 Query: 224 QNLVGEEDRGWSCAKYLLTYERTNI--AGIGFSVAALQRLKKIAATQRSHG 272 N+VG+ G S + ++ I A +G A + A + R G Sbjct: 242 SNIVGKPGDGISMLEAAFSWTAALIGSACVGVMRTAFDIALRFAQSDRRSG 292 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,309,773 Number of extensions: 57094 Number of successful extensions: 159 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 7 Length of query: 398 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 311 Effective length of database: 1,207,925 Effective search space: 375664675 Effective search space used: 375664675 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)