BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0189.p (384 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 130 2e-31 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 119 4e-28 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 105 6e-24 gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 67 2e-12 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 66 5e-12 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 130 bits (328), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 108/340 (31%), Positives = 160/340 (47%), Gaps = 13/340 (3%) Query: 42 DMWSQLAEMGVIGALFREEDGGYGGGGFDIAVVFEEIGRGLL-LEPLLGSAILVGEAIAA 100 D+ ++ E+G G E DGG G D VVFEE+ R L +V IA+ Sbjct: 40 DVIAKAGELGFCGLYTPEADGGLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIAS 99 Query: 101 AGTPARKA-LLEQIIAGQTLAALAHEEADSHYAPTHVXHVATRATQSADGGWRLQGHKAV 159 G P+ KA EQ+ AG+ + + E S + A + DG + L G KA Sbjct: 100 WGQPSLKAQWCEQLAAGRKIGSYCLTEPGSGSDAASLKTTA----RLEDGHYVLNGSKAF 155 Query: 160 VVHAEAADVFLVSARTSGETDDPDGISLFLIPADTAGLQLQGSDTIDGGR---VADLLLQ 216 + A + DV +V ART GE G+S ++P + G+ ++ G + Sbjct: 156 ISGAGSTDVLVVMARTGGE--GARGVSAIVVPGNAPGVSYGKKESKMGWNSQPTRTISFD 213 Query: 217 DAALPADALLGSPGDGAALLERILGRGVLALCAEALGAMEVAKEATLDYLRTRKQFGLPI 276 + +PAD LLG G G + L G + + ++GA + A +A Y++ RKQFG PI Sbjct: 214 NVKVPADHLLGQEGQGFIFAMKGLDGGRINIATCSVGAAQAALDAAHAYMKERKQFGRPI 273 Query: 277 GSFQALQHRMADLLLEVEQARSAVINAAAALDADRLTRERALSAAKFSIGRIGTLVAEES 336 FQALQ ++AD+ E+ AR V AA LDA + AK +G V E+ Sbjct: 274 ADFQALQFKLADMQTELVAARQMVRLAATKLDAGSPEATTYCAMAKRLATDLGFRVCNEA 333 Query: 337 IQLHGGIGMTWELPLSHYAKRLVMIDHQLGDEDHHLQRYI 376 +QLHGG G E PL Y + + + HQ+ + + + R I Sbjct: 334 LQLHGGYGYIREYPLERYFRDVRV--HQILEGTNEIMRVI 371 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 119 bits (298), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 112/393 (28%), Positives = 183/393 (46%), Gaps = 37/393 (9%) Query: 6 TEERRMLADSLRRLITEQY-GFEA---RKHLLQSAPGMSRDMWSQLAEMGVIGALFREED 61 + E M+ D++R E+ F A R H + + +LAE+G +G E+ Sbjct: 4 SPEHEMIRDAMRHFAQERLLPFAADWDRNHTFPA------EALKELAELGAMGMCVPEQW 57 Query: 62 GGYGGGGFDIAVVFEEIGRGLLLEPLLGSAILVGEAIAAA-----GTPARK-ALLEQIIA 115 GG G + + EEI G + + + V ++A G+P +K L+ + Sbjct: 58 GGAGMDYMSLVLALEEIAAG---DGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLAR 114 Query: 116 GQTLAALAHEEADSHYAPTHVXHVATRATQSADGGWRLQGHKAVVV---HAEAADVFLVS 172 G+ L E + + + TRA + D + L G K + HA A VF V+ Sbjct: 115 GEMLGCFCLTEP---HTGSDAAAIRTRAERDGDD-FILNGTKQFITSGKHAGVAIVFAVT 170 Query: 173 ARTSGETDDPDGISLFLIPADTAGLQLQGSDTIDGGRVAD---LLLQDAALPADALLGSP 229 R++G+ GIS FL+P DT G + S+ G +D ++L + +PA ALLG Sbjct: 171 DRSAGKK----GISCFLVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKE 226 Query: 230 GDGAALLERILGRGVLALCAEALGAMEVAKEATLDYLRTRKQFGLPIGSFQALQHRMADL 289 G+G + L G + + A+++G A EA + Y R R+ FG+PI QA+ R+AD+ Sbjct: 227 GEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADM 286 Query: 290 LLEVEQARSAVINAAAALDADRLTRERALSAAKFSIGRIGTLVAEESIQLHGGIGMTWEL 349 ++ AR V AA D R + A S AK +A ++IQ+HGG+G T + Sbjct: 287 NTLLDAARLMVWRAAQLKDQGRPCLKEA-SMAKMFASEAAEKIASDAIQIHGGVGYTSDF 345 Query: 350 PLSHYAKRLVMIDHQLGDEDHHLQRYIALGREV 382 P+ + + + G D +QR + +GR + Sbjct: 346 PVERIYRDVRICQIYEGAND--IQRLV-IGRAI 375 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 105 bits (262), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 100/354 (28%), Positives = 161/354 (45%), Gaps = 19/354 (5%) Query: 42 DMWSQLAEMGVIGALFREEDGGYGGGGFDIAVVFEEIGR---GLLLEPLLGSAILVGEAI 98 D+W +L ++GV+G EE GG G G + EEI R + L S + V + Sbjct: 46 DLWKKLGDLGVLGITVSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIK 105 Query: 99 AAAGTPARKALLEQIIAGQTLAALAHEEADSHYAPTHVXHVATRATQSADGGWRLQGHKA 158 ++ L ++I+G + ALA E + A + V + RA + D + L G K Sbjct: 106 RNGNEEQKRKYLPRLISGDFIGALAMSEPN---AGSDVVSMKLRADKKGDR-YVLNGSKM 161 Query: 159 VVVHAEAADVFLVSARTSGETDDPDGISLFLIPADTAGLQL-QGSDTID--GGRVADLLL 215 + + ADV +V A+T E G++ FL+ G + Q D + G +L+ Sbjct: 162 WITNGPDADVLVVYAKTDLEAG-ARGMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVF 220 Query: 216 QDAALPADALLGSPGDGAALLERILGRGVLALCAEALGAMEVAKEATLDYLRTRKQFGLP 275 QD +P + +LG G G +L L L LG M+ + + Y+ RKQFG Sbjct: 221 QDCEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPLGIMQACMDVVVPYVHDRKQFGQA 280 Query: 276 IGSFQALQHRMADLLLEVEQARSAVINAAAALD-ADRLTRERAL----SAAKFSIGRIGT 330 IG FQ +Q ++AD+ + ++ V A D AD + RAL + A T Sbjct: 281 IGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRADSADKVRALRKDAAGAILYSAEKAT 340 Query: 331 LVAEESIQLHGGIGMTWELPLSHYAKRLVMIDHQLGDEDHHLQRYIALGREVPA 384 +A E+IQ GG G E P+ + + +++G ++R + +GRE+ A Sbjct: 341 WMAGEAIQSLGGNGYINEYPVGRLWRDAKL--YEIGAGTSEIRRML-IGRELFA 391 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 67.4 bits (163), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 79/324 (24%), Positives = 136/324 (41%), Gaps = 13/324 (4%) Query: 33 LQSAPGMSRDMWSQLAEMGVIGALFREEDGGYGGGGFDIAVVFEEIGR-GLLLEPLLGSA 91 + A G L + G + AL +E GG G G + +V+ EEI R G G Sbjct: 32 IDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLGLTEASVIMEEINRTGGNSGACHGQM 91 Query: 92 ILVGEAIAAAGTPARKALLEQIIAGQ-TLAALAHEEADSHYAPTHVXHVATRATQSADGG 150 +G + ++ L +I +G+ L ++A E + T + VA + Sbjct: 92 YNMGTLLRHGSPEQKERYLPRIASGELRLQSMAVTEPTTGTDTTRIKTVAVKKGDR---- 147 Query: 151 WRLQGHKAVVVHAEAADVFLVSARTSG---ETDDPDGISLFLIPADTA---GLQLQGSDT 204 + + G K + + +D+ ++ ART+ + +G+S+FL+ A G++++ Sbjct: 148 YVINGQKVWISRVQHSDLMILLARTTPLEQVSRKSEGMSIFLVDLKEAIGHGMEVRPILN 207 Query: 205 IDGGRVADLLLQDAALPADALLGSPGDGAALLERILGRGVLALCAEALGAMEVAKEATLD 264 + +L + +PA+ L+G G G + L + AE +G + Sbjct: 208 MVNHETNELFFDNLEIPAENLIGEEGRGFRYILDGLNAERTLIAAECIGDGYWFIDKATQ 267 Query: 265 YLRTRKQFGLPIGSFQALQHRMADLLLEVEQARSAVINAAAALDADRLTRERALSAAKFS 324 Y R R F PIG Q +Q +A+ +EVE A A A DA + +A + AK+ Sbjct: 268 YARERVVFDRPIGMNQGVQFPLAEAYIEVEAANLMRYQACALFDAQQSCGAQA-NMAKYL 326 Query: 325 IGRIGTLVAEESIQLHGGIGMTWE 348 + A +Q HGG G E Sbjct: 327 AAKAAWEAANVCLQTHGGFGFACE 350 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 65.9 bits (159), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 72/285 (25%), Positives = 124/285 (43%), Gaps = 18/285 (6%) Query: 40 SRDMWSQLAEMGVIGALFREEDGGYGGGGFDIAVVFEEIGRGLLLEP--LLGSAILVGEA 97 +R + +A+ G I AL E GG D A+ EE+ + P LLG+ + + A Sbjct: 46 TRPFYQAMADAGFIKALLPESFGGTALSTLDFALAAEELTAVDVNVPSALLGTGLGL-HA 104 Query: 98 IAAAGTPARKA--LLEQIIAGQTLAALAHEEAD--SHYAPTHVX-HVATRATQSADGGWR 152 + GTP +K L + I G LAALA E ++Y T V T A D W Sbjct: 105 VVHHGTPEQKQRFLPDFIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDE-WV 163 Query: 153 LQGHKAVVVHAEAAD-----VFLVSARTSGETDDPDGISLFLIPADTAGLQLQGSDTIDG 207 + G K + D ++ V RT +++ ++P + G+++ G G Sbjct: 164 INGAKHYTTNGTGWDGKGAHLYSVICRTDPALPPQQSLAVIMVPGNAPGIEIVGLLDTMG 223 Query: 208 GRVA---DLLLQDAALPADALLGSPGDGAALLERILGRGVLALCAEALGAMEVAKEATLD 264 R + + +PA ++G PGDG ++LE + + +G M A + L Sbjct: 224 HRATISPRVHFNNVRVPASNIVGKPGDGISMLEAAFSWTAALIGSACVGVMRTAFDIALR 283 Query: 265 YLRTRKQFG-LPIGSFQALQHRMADLLLEVEQARSAVINAAAALD 308 + ++ ++ G +P+ Q + +AD+ + +E AR + LD Sbjct: 284 FAQSDRRSGTIPVIEHQNVGFMLADIKMRIEAARYLTWKSCHQLD 328 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,132,379 Number of extensions: 45338 Number of successful extensions: 197 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 189 Number of HSP's successfully gapped: 7 Length of query: 384 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 297 Effective length of database: 1,207,925 Effective search space: 358753725 Effective search space used: 358753725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)