BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0186.p (397 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 215 5e-57 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 194 1e-50 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 137 1e-33 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 90 4e-19 gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 86 6e-18 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 215 bits (548), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 133/378 (35%), Positives = 207/378 (54%), Gaps = 13/378 (3%) Query: 24 DDERQVRDAAQAYCQDRLAPRVLEAFRHEKMDVSIFREMGELGLLGATIDEQYGGAGLNY 83 +D +R+A A+ +APR E R ++ + +++++G+LG+LG T+ E+YGGAGL Y Sbjct: 13 EDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYGGAGLGY 72 Query: 84 VCYGLIAREVERVDSGYRSMMSVQSSLVMTPINAFGTEAQKQKYLPKLASGEWIGCFGLT 143 + + + E+ R + S+L + I G E QK+KYLP+L SG++IG ++ Sbjct: 73 LAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDFIGALAMS 132 Query: 144 EPNHGSDPGSMITRARKVDGGYSLSGSKMWITNSPVADVFVVWAKDD-EGEIRG---FIL 199 EPN GSD SM RA K Y L+GSKMWITN P ADV VV+AK D E RG F++ Sbjct: 133 EPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLEAGARGMTAFLV 192 Query: 200 DKGSKGLSAPAIHGKVGLRSSITGEIVMDNVFCPEENAFPDV-RGLKGPFTCLNSARYGI 258 +KG KG S K+G+R S TGE+V + PEEN V RG+ + L+ R + Sbjct: 193 EKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLDFERSVL 252 Query: 259 AWGALGAAEDCYERARRYTMERQQFGRPLAANQLVQKKLADMLTEISLGLQGCLRLGRLK 318 + G LG + C + Y +R+QFG+ + QL+Q KLADM + + +G+ Sbjct: 253 SGGPLGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQAC 312 Query: 319 DEGYSGIEQTSIMKRNSCGKALDIARMARDM-------MGGNGISDEFGVARHLVNLEVV 371 D S ++ +++++ G L A A M +GGNG +E+ V R + ++ Sbjct: 313 DRADSA-DKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNGYINEYPVGRLWRDAKLY 371 Query: 372 NTYEGTHDVHALILGRAI 389 GT ++ +++GR + Sbjct: 372 EIGAGTSEIRRMLIGREL 389 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 194 bits (493), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 120/373 (32%), Positives = 195/373 (52%), Gaps = 5/373 (1%) Query: 22 LSDDERQVRDAAQAYCQDRLAPRVLEAFRHEKMDVSIFREMGELGLLGATIDEQYGGAGL 81 LS + +RDA + + Q+RL P + R+ +E+ ELG +G + EQ+GGAG+ Sbjct: 3 LSPEHEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGM 62 Query: 82 NYVCYGLIAREVERVDSGYRSMMSVQSSLVMTPINAFGTEAQKQKYLPKLASGEWIGCFG 141 +Y+ L E+ D +++SVQ+SL +G+ QK+++L LA GE +GCF Sbjct: 63 DYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGCFC 122 Query: 142 LTEPNHGSDPGSMITRARKVDGGYSLSGSKMWITNSPVADVFVVWAKDDEGE----IRGF 197 LTEP+ GSD ++ TRA + + L+G+K +IT+ A V +V+A D I F Sbjct: 123 LTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCF 182 Query: 198 ILDKGSKGLSAPAIHGKVGLRSSITGEIVMDNVFCPEENAF-PDVRGLKGPFTCLNSARY 256 ++ + G K+G +S T +I++DN P + G + + L + R Sbjct: 183 LVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRI 242 Query: 257 GIAWGALGAAEDCYERARRYTMERQQFGRPLAANQLVQKKLADMLTEISLGLQGCLRLGR 316 GIA ++G A +E A Y +R+ FG P+ +Q V +LADM T + R + Sbjct: 243 GIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRAAQ 302 Query: 317 LKDEGYSGIEQTSIMKRNSCGKALDIARMARDMMGGNGISDEFGVARHLVNLEVVNTYEG 376 LKD+G +++ S+ K + A IA A + GG G + +F V R ++ + YEG Sbjct: 303 LKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYEG 362 Query: 377 THDVHALILGRAI 389 +D+ L++GRAI Sbjct: 363 ANDIQRLVIGRAI 375 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 137 bits (346), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 110/378 (29%), Positives = 186/378 (49%), Gaps = 8/378 (2%) Query: 18 LEQQLSDDERQVRDAAQAYCQDRLAPRVLEAFRHEKMDVSIFREMGELGLLGATIDEQYG 77 ++ +L+ D+ + +A+ + +AP + + + + GELG G E G Sbjct: 1 MDFELNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADG 60 Query: 78 GAGLNYVCYGLIAREVERVDSGYRSMMSVQSSLVMTPINAFGTEAQKQKYLPKLASGEWI 137 G GL+ + ++ E+ R + + +++ + +V I ++G + K ++ +LA+G I Sbjct: 61 GLGLSRLDATVVFEELARACTSTAAYLTIHN-MVSWMIASWGQPSLKAQWCEQLAAGRKI 119 Query: 138 GCFGLTEPNHGSDPGSMITRARKVDGGYSLSGSKMWITNSPVADVFVVWAKDDEGEIRG- 196 G + LTEP GSD S+ T AR DG Y L+GSK +I+ + DV VV A+ RG Sbjct: 120 GSYCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGEGARGV 179 Query: 197 --FILDKGSKGLSAPAIHGKVGLRSSITGEIVMDNVFCPEENAF-PDVRGLKGPFTCLNS 253 ++ + G+S K+G S T I DNV P ++ + +G L+ Sbjct: 180 SAIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDG 239 Query: 254 ARYGIAWGALGAAEDCYERARRYTMERQQFGRPLAANQLVQKKLADMLTEISLGLQGCLR 313 R IA ++GAA+ + A Y ER+QFGRP+A Q +Q KLADM TE+ Q +R Sbjct: 240 GRINIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQ-MVR 298 Query: 314 LGRLKDEGYSGIEQT--SIMKRNSCGKALDIARMARDMMGGNGISDEFGVARHLVNLEVV 371 L K + S T ++ KR + + A + GG G E+ + R+ ++ V Sbjct: 299 LAATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVH 358 Query: 372 NTYEGTHDVHALILGRAI 389 EGT+++ +I+ R + Sbjct: 359 QILEGTNEIMRVIISRKL 376 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 89.7 bits (221), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 96/356 (26%), Positives = 153/356 (42%), Gaps = 45/356 (12%) Query: 75 QYGGAGLNYVCYGLIAREVERVDSGYRSMMSVQSSLVMTPINAFGTEAQKQKYLPKLASG 134 ++GG GL +V + + + G+ +M + A G+ A + +YL K+ASG Sbjct: 100 EHGGQGLPHVLDCALYEMLSAANHGW-TMYPGLLHGAYACLAAHGSAALQARYLDKIASG 158 Query: 135 EWIGCFGLTEPNHGSDPGSMITRA-RKVDGGYSLSGSKMWITN-----SP-VADVFVVWA 187 EW+ LTE GSD G + TRA + DG Y LSG K++I+ +P + + + Sbjct: 159 EWLATMCLTEAQAGSDLGQLRTRAVPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARL 218 Query: 188 KDDEGEIRGF-------ILDKGSK-GLSAPAIHGKVGLRSSITGEIVMDNV---FCPEEN 236 D +G +LD GS+ G+ I K+G+ S T + + E Sbjct: 219 PDAPAGSKGLSLFLAPRVLDDGSRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLVGEAG 278 Query: 237 AFPDVRGLKGPFTCLNSARYGIAWGALGAAEDCYERARRYTMERQQFGRP-LAANQLV-- 293 RGL F +N+AR + LG AE Y+RA Y ER+Q P + N L+ Sbjct: 279 -----RGLAAMFVMMNAARLHVGAQGLGLAEAAYQRASAYARERRQSRAPGSSGNDLIVH 333 Query: 294 QKKLADMLTEISLGLQGCLRL----GRLKDEGYSGIEQ-------------TSIMKRNSC 336 + +L E ++G L G L D E T ++K Sbjct: 334 HPAVQKLLMEQRCRIEGARLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVIKAFLT 393 Query: 337 GKALDIARMARDMMGGNGISDEFGVARHLVNLEVVNTYEGTHDVHAL-ILGRAITG 391 + A A + GG+G E G+ + + + + YEGT+++ A+ L R + G Sbjct: 394 DQGFQCASRALQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRKVLG 449 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 85.9 bits (211), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 103/392 (26%), Positives = 167/392 (42%), Gaps = 79/392 (20%) Query: 59 FREMGELGLLGATIDEQYGGAGLNYVCYGLIAREVERVDSGYRSMMSVQSSLVMTP---- 114 +++ E G + D ++GG GL +S Y + S + M P Sbjct: 85 YQQYVEAGWAALSCDPEFGGQGLPLTIN----------NSFYEMLNSSNQAWTMYPGLSH 134 Query: 115 -----INAFGTEAQKQKYLPKLASGEWIGCFGLTEPNHGSDPGSMITRAR-KVDGGYSLS 168 ++A G+E QKQ YLPKL SGEW G LTE + G+D G + ++A + DG Y ++ Sbjct: 135 GAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLRSKAEPQADGSYKIT 194 Query: 169 GSKMWIT---NSPVADVF-VVWAK-DDEGE----IRGFILDK------GSKGLSAP---- 209 G+K++I+ N A++ +V A+ D E I F++ K GS G P Sbjct: 195 GNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPNADGSLGARNPIFCG 254 Query: 210 AIHGKVGLRSSITGEIVMDN-----VFCPEENAFPDVRGLKGPFTCLNSARYGIAWGALG 264 AI K+G+ + T ++ +D + P +GL+ F +N+AR G+ LG Sbjct: 255 AIEEKMGIHGNATCQMNLDGATGWLIGQPH-------KGLQAMFVFMNAARLGVGMQGLG 307 Query: 265 AAEDCYERARRYTMERQQF------------GRPLAANQLVQKKL--ADMLTEISLGLQG 310 E Y+ A Y +R Q P+ + V++ L A E Sbjct: 308 LTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVHPDVRRMLLTAKAFAEGGRAFTS 367 Query: 311 CLRLGRLKDEGYSGIEQ------------TSIMKRNSCGKALDIARMARDMMGGNGISDE 358 + L L E EQ T ++K A + GG+G E Sbjct: 368 YVAL-ELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYGGHGYIAE 426 Query: 359 FGVARHLVNLEVVNTYEGTHDVHAL-ILGRAI 389 +G+ +++ + + YEGT+ + +L +LGR I Sbjct: 427 WGMEQYVRDARINMIYEGTNTIQSLDLLGRKI 458 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,233,954 Number of extensions: 51496 Number of successful extensions: 155 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 136 Number of HSP's successfully gapped: 10 Length of query: 397 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 310 Effective length of database: 1,207,925 Effective search space: 374456750 Effective search space used: 374456750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)