BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0185.p (303 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312894|ref|YP_003776986.1| LysR family transcription regul... 209 2e-55 gi|300309845|ref|YP_003773937.1| LysR family transcription regul... 156 2e-39 gi|300310443|ref|YP_003774535.1| LysR family transcription regul... 142 4e-35 gi|300310906|ref|YP_003774998.1| LysR family transcription regul... 139 3e-34 gi|300312645|ref|YP_003776737.1| LysR family transcription regul... 134 1e-32 >gi|300312894|ref|YP_003776986.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 299 Score = 209 bits (533), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 115/296 (38%), Positives = 175/296 (59%), Gaps = 5/296 (1%) Query: 1 MRRKIPSTQAMAVFESAARHQSFTKAAQEMAVTQSAVCRQVAALEEFLGLKLFRRTKRGV 60 MRR+IPS ++ FE+AARH SF +AA+E+A+T+ A+ RQ++ LE FLG+ LF R V Sbjct: 1 MRRRIPSNSSLMAFEAAARHGSFARAAEELALTEGAISRQISRLEGFLGVTLFERIGNRV 60 Query: 61 RLTEAGVSYSHQVGQRLDEIERDALHLMSRDGHGDTLELGVMPTFATRWLLPRLRSFLEA 120 RL G Y+ V + L+ ++RD+ HLMS+ +G +L++ V PTFA RWL+PRL F Sbjct: 61 RLLPHGQRYASHVRETLERLDRDSQHLMSQPPNGASLDIAVSPTFAMRWLIPRLPRFAAL 120 Query: 121 HPGVTVNLRPRTRPFLFEETNLDATLYAGQASWPGTEGVFLMRESMVAVASPELIGAGRK 180 HP +TV+L R PFL + LDA ++ +W G + L+ E +V V P L+ AG Sbjct: 121 HPHITVHLSERMEPFLLPGSGLDAAIHFLHPAWTGMQTYSLLDEVLVPVCHPALV-AGTS 179 Query: 181 LTPTELLAYPLLQQSTRPYVWRQWFASFGMQVQNDLVGTQLELFSMLTQAAIVGMGVALI 240 P L A P L + W+++ A G+ + + G + +L ML +AA+ G+GVAL+ Sbjct: 180 GVPA-LDALPRLHRRQNHDAWQRYAAQTGISLSHSAQGPRYDLHGMLIEAALAGLGVALV 238 Query: 241 PRFLIEDELRRGLLVVAVDHDYLSTHSYYLIYPE--RTSERPLLRLFQEWLEEQAK 294 PR +E EL +G LV D + ++ L+ PE + P L+ F +W+ E++ Sbjct: 239 PRLYVETELAQGRLVAPWPQDSRISKTFCLVLPEPLQLGSEP-LKAFAQWILEESS 293 >gi|300309845|ref|YP_003773937.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 294 Score = 156 bits (394), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 96/292 (32%), Positives = 149/292 (51%), Gaps = 3/292 (1%) Query: 5 IPSTQAMAVFESAARHQSFTKAAQEMAVTQSAVCRQVAALEEFLGLKLFRRTKRGVRLTE 64 IP ++ FE+ AR +SF AA E+ +T SA+ QVA LE+ LGL+LF R+ +GV LT Sbjct: 2 IPPLTSLMAFEAIARRKSFNLAAAELHLTPSAISHQVARLEKLLGLRLFERSTKGVELTP 61 Query: 65 AGVSYSHQVGQRLDEIERDALHLMSRDGHGDTLELGVMPTFATRWLLPRLRSFLEAHPGV 124 AG SY ++V L I L R G ++L++ P+FA+ WL+PR+ F + HP + Sbjct: 62 AGQSYLNRVAGALGAINSATDDL--RHGVENSLQIHSSPSFASLWLMPRIGRFTQRHPDI 119 Query: 125 TVNLRPRTRPFLFEETNLDATLYAGQASWPGTEGVFLMRESMVAVASPELIGAGRKLTPT 184 + L FE +D + G WP E ++ E ++ +ASP+ + R TP Sbjct: 120 ALGLSASPEISDFEIGQVDLDIRCGVPHWPDLEIEAVLSERVIPMASPDFLARHRIDTPD 179 Query: 185 ELLAYPLLQQSTRPYVWRQWFASFGMQVQNDLVGTQLELFSMLTQAAIVGMGVALIPRFL 244 +L+ PL+Q W +WFA + ++ + + + + M +AA+ G+GV Sbjct: 180 DLVLAPLIQSRGSLLQWPEWFARYAPDLRPERIALRFDRAMMSIEAAVQGLGVVFESDAF 239 Query: 245 IEDELRRG-LLVVAVDHDYLSTHSYYLIYPERTSERPLLRLFQEWLEEQAKA 295 L G L V D L +Y + P R RP + LF +WL E++ A Sbjct: 240 GAVALASGRLQPVFADGLALEITQHYAVCPARNKSRPEVMLFLQWLREESGA 291 >gi|300310443|ref|YP_003774535.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 295 Score = 142 bits (357), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 97/296 (32%), Positives = 145/296 (48%), Gaps = 18/296 (6%) Query: 5 IPSTQAMAVFESAARHQSFTKAAQEMAVTQSAVCRQVAALEEFLGLKLFRRTKRGVRLTE 64 +P+ QA FE+ AR +SF AA E+ +T SAV QVA LE +LG++LF R+ GVR+T Sbjct: 7 LPNLQA---FEAVARRKSFALAAAELHLTASAVSHQVARLESYLGIRLFERSAHGVRITT 63 Query: 65 AGVSYSHQVGQRLDEIERDALHLMSRDGHGDTLELGVMPTFATRWLLPRLRSFLEAHPGV 124 AG +Y ++ + I ++ + R G +TL + P+ A+ WL+ RL F ++P + Sbjct: 64 AGEAYLARIQGAISAITAASMDV--RQGVSNTLYIHSAPSIASLWLMARLNDFARSYPEI 121 Query: 125 TVNLRPRTRPFLFEETNLDATLYAGQASWPGTEGVFLMRESMVAVASPELIGAGRKLTPT 184 T NL FE +D + G +WPG L E +V +ASP I R P Sbjct: 122 TFNLSAAHSQSNFELGEVDIDIRYGIPNWPGLVVEPLFEERIVPLASPAFIREYRLRKPA 181 Query: 185 ELLAYPLLQQSTRPYVWRQWFASFGMQVQNDLVGTQLELFSMLTQAAIVGMGVAL----- 239 +LL L++ + W WF F + + + M AA G+GVAL Sbjct: 182 DLLGVKLIRSNVSVVQWADWFGEFADVRAPEQFAVRFDRAQMSLDAATQGLGVALESATV 241 Query: 240 IPRFLIEDELRR--GLLVVAVDHDYLSTHSYYLIYPERTSERPLLRLFQEWLEEQA 293 R L E +L+ GL + +++ +YP + RP + F EWL EQA Sbjct: 242 AARHLEEKKLKPIFGL------ERAIKVKAHFAVYPAHHARRPAVAAFLEWLREQA 291 >gi|300310906|ref|YP_003774998.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 300 Score = 139 bits (349), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 98/285 (34%), Positives = 141/285 (49%), Gaps = 6/285 (2%) Query: 9 QAMAVFESAARHQSFTKAAQEMAVTQSAVCRQVAALEEFLGLKLFRRTKRGVRLTEAGVS 68 A+ F +AARHQSFT AA E+ VT A+ RQ+ LE +LG+ LF R R V LTE G Sbjct: 9 NAIRAFLAAARHQSFTLAAHELNVTHGAISRQIKNLEGYLGMPLFERRTRQVLLTERGRQ 68 Query: 69 YSHQVGQRLDEIERDALHLMSRDGHGDTLELGVMPTFATRWLLPRLRSFLEAHPGVTVNL 128 + + L EI +A + RD T+ + V P+FA RWL+P L F+E +PG+ + Sbjct: 69 FFEEASPAL-EILANAARRVMRDAPTRTVVINVRPSFAVRWLIPHLSDFVERYPGIAPQV 127 Query: 129 RPRTRPFLFEETNLDATLYAGQASWPGTEGV-FLMRESMVAVASPELIGAGRKLTPTELL 187 T ++ D + G+ +WP T V M + +V VASP L+ A + L Sbjct: 128 VTSTATVGKAGSSFDIAVRRGETNWPETLKVQRFMEDELVLVASPALLQAMPLEDVSGLA 187 Query: 188 AYPLLQQSTRPYVWRQWFASFGMQVQNDLVGTQLELFSMLTQAAIVGMGVALIPRFLIED 247 + +L +R W W G++ + + QA + G+GVAL P L ++ Sbjct: 188 RHTVLLSRSRRNDWEDWRKLAGLKRSRSQAMLHFDHVHFVLQAVVDGLGVALSPVSLADN 247 Query: 248 ELRRGLLVVAVDHDYLSTHSYYL-IYPERTSERPLLRLFQEWLEE 291 +LR G LV L+ YYL I + SE +LF WL E Sbjct: 248 DLRSGRLVCPFPDLRLTLAPYYLGINAQAGSE---AQLFARWLAE 289 >gi|300312645|ref|YP_003776737.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 287 Score = 134 bits (336), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 99/287 (34%), Positives = 145/287 (50%), Gaps = 6/287 (2%) Query: 9 QAMAVFESAARHQSFTKAAQEMAVTQSAVCRQVAALEEFLGLKLFRRTKRGVRLTEAGVS 68 A+ FE +ARH SFT+AA+E++VTQ+AV QV LE+ LG+ LFRR RG+ LT+ G++ Sbjct: 6 NALRAFEVSARHLSFTRAAEELSVTQTAVSMQVKNLEQRLGVALFRRLPRGLALTDEGLA 65 Query: 69 YSHQVGQRLDEIERDALHLMSRDGHGDTLELGVMPTFATRWLLPRLRSFLEAHPGVTVNL 128 V Q I D L + L +GV+ TFA WL+PRLR F + HP V + L Sbjct: 66 LLPVVAQSFGRIA-DVLAQFEDGRRREVLTVGVVGTFAVGWLMPRLRQFQQQHPFVDLRL 124 Query: 129 RPRTRPFLFEETNLDATLYAGQASWPGTEGVFLMRESMVAVASPELIGAGRKLTPTELLA 188 LD + G +W GT+ L + + +P + A R +P +L A Sbjct: 125 LTNNNRVDLAGEGLDYAIRFGDGAWHGTQAERLFEAPLAPMCAPAI--AARLRSPADLGA 182 Query: 189 YPLLQQSTRPYVWRQWFASFGMQVQNDLVGTQLELFSMLTQAAIVGMGVALIPRFLIEDE 248 LL +S R W +WFA+ G L G + L +AA GVAL+P + E E Sbjct: 183 ETLL-RSYRSDEWGRWFAAAGAACP-PLRGFVFDSSLALAEAAAQEAGVALLPVLMFERE 240 Query: 249 LRRGLLVVAVDHDYLSTHSYYLIYPERTSERPLLRLFQEWLEEQAKA 295 L++G LV + +Y+L + + + F++WL+ A A Sbjct: 241 LQQGRLVCPFGQR-IVLGAYWLTSLKSRPQTAAMAAFRQWLQSHAGA 286 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 959,232 Number of extensions: 40723 Number of successful extensions: 256 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 217 Number of HSP's successfully gapped: 49 Length of query: 303 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 218 Effective length of database: 1,217,395 Effective search space: 265392110 Effective search space used: 265392110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)