BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0183.p (212 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312440|ref|YP_003776532.1| threonine efflux protein [Herba... 66 3e-12 >gi|300312440|ref|YP_003776532.1| threonine efflux protein [Herbaspirillum seropedicae SmR1] Length = 208 Score = 65.9 bits (159), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 62/195 (31%), Positives = 91/195 (46%), Gaps = 8/195 (4%) Query: 18 TMSPGPSFVMVARTSVGGGRANGLLAALGMGIGGVLFAGAALLGLQAVLLAVPSLYLVLK 77 T++PGP + V + GR GL+AA G G V A+LG A++ A P Y L+ Sbjct: 15 TLAPGPDNLYVLTRGIAQGRKAGLVAAAGFCSGLVFHTLLAVLGFAALIKAYPPAYHALQ 74 Query: 78 FGGGVYLAYMGWRIWRGARTPLMADDIALRDASPSVSRRSLWLGLVTQISNPKTAIAYTS 137 + G YLAY+G R R A L + A+ RR W ++ + NPK + + + Sbjct: 75 YAGAAYLAYLGVRTLRSASAGLALAADG-QGAAAVPLRRIYWQSVLANMLNPKVTLFFIA 133 Query: 138 VFAAFLPAETSLP-FKGLTLLLVFLIEAGWYGLVAVALSS----ARPRRWYVQGKHWFDR 192 F+ E + L L LVF+++A + A+AL S A RR H +R Sbjct: 134 FLPQFVQREAGHEALQMLVLALVFIVQA-FAIFSAIALFSGAVGAYFRRRASASVH-LNR 191 Query: 193 TAGAVMMTLGFRLLL 207 AG + LG R+ L Sbjct: 192 LAGCAFIGLGIRMAL 206 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.139 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 551,356 Number of extensions: 19776 Number of successful extensions: 103 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 100 Number of HSP's successfully gapped: 5 Length of query: 212 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 131 Effective length of database: 1,236,335 Effective search space: 161959885 Effective search space used: 161959885 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 128 (53.9 bits)