BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0180.p (406 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspiri... 786 0.0 gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum ... 367 e-103 gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoac... 340 2e-94 >gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspirillum seropedicae SmR1] Length = 403 Score = 786 bits (2030), Expect = 0.0, Method: Compositional matrix adjust. Identities = 378/403 (93%), Positives = 390/403 (96%) Query: 4 MEHTEHSHDSLHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVQELQALGYEVTRGLGGT 63 MEHT H HD+LHQHRAHWAGLRRDLHAHPELRFEEHRTADVVV+ELQ+LGY VTRGLGGT Sbjct: 1 MEHTGHQHDTLHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGT 60 Query: 64 GVVASLPGSDPQRGIVLRADLDALPIVEANDFAHASCSHGIMHACGHDGHTVMLLGAARV 123 GVVASL G+DPQRGIVLRADLDALPIVEANDFAHASC+HG+MHACGHDGHTVMLLGAAR+ Sbjct: 61 GVVASLAGADPQRGIVLRADLDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARL 120 Query: 124 LKQQPTLPGSVHFVFQPGEEGGAGARKMIDDGLFDQFPTEAVFGMHNWPGLPAGQFGLRT 183 LKQ P LPGSVHFVFQPGEEGGAGARKMIDDGLF+QFPTEAVFGMHNWPGLPAGQFGLR Sbjct: 121 LKQGPMLPGSVHFVFQPGEEGGAGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGLRP 180 Query: 184 GPIMAAGSRLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVC 243 GPIMAAGSRLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVC Sbjct: 181 GPIMAAGSRLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVC 240 Query: 244 MFHAGDTDNVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCHALADAYGAKVEVEFFQYYS 303 MFHAGDTDNVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCHALA+AYGAKVEVEFFQYY Sbjct: 241 MFHAGDTDNVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVEFFQYYP 300 Query: 304 ATINTPTETDFCERVIRQTFGDERIRTGIPANMTSEDFGFMLEERPGTYVLIGNAPQDRA 363 ATINTP ETDFCERVIRQTFGD+RIRTGIPANMTSEDFGFMLEERPG YVLIGNAPQDRA Sbjct: 301 ATINTPAETDFCERVIRQTFGDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQDRA 360 Query: 364 SHSLHHPHYDFNDDIIEEGVRYWVALAQSWFGSGLKGGALTSR 406 SHSLHHPHYDFNDDIIE GVRYWVALAQ +FGSGLKG A+TSR Sbjct: 361 SHSLHHPHYDFNDDIIEAGVRYWVALAQGYFGSGLKGEAVTSR 403 >gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum seropedicae SmR1] Length = 402 Score = 367 bits (942), Expect = e-103, Method: Compositional matrix adjust. Identities = 181/380 (47%), Positives = 235/380 (61%), Gaps = 8/380 (2%) Query: 24 LRRDLHAHPELRFEEHRTADVVVQELQALGYEVTRGLGGTGVVASLPGSDPQRGIVLRAD 83 +RR LHAHPELRFEE RTA V L++ G V +GG GVVA+L R I LRAD Sbjct: 20 IRRHLHAHPELRFEEKRTASFVADMLRSYGLTVAENIGGYGVVATLRKGKSTRAIALRAD 79 Query: 84 LDALPIVEANDFAHASCSHGIMHACGHDGHTVMLLGAARVLKQQPTLPGSVHFVFQPGEE 143 +DALP+ E NDF+H S G MHACGHDGHT MLLGAAR L ++ G+VHFVFQP EE Sbjct: 80 MDALPMSEQNDFSHISTCAGKMHACGHDGHTTMLLGAARRLSREVEFDGTVHFVFQPAEE 139 Query: 144 GGAGARKMIDDGLFDQFPTEAVFGMHNWPGLPAGQFGLRTGPIMAAGSRLKITITGKGAH 203 GGAGAR MI+DGLF++FP +A+FG+HNWPGLPAG FGLR GP+MA+ + T+ G+GAH Sbjct: 140 GGAGARLMIEDGLFERFPADAIFGVHNWPGLPAGSFGLRPGPLMASSNTFSATLFGRGAH 199 Query: 204 AAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTDNVIPDRAELRGT 263 AQPH +DP+ A + L Q+I R+ +P AVISV H G DNVIP++A L GT Sbjct: 200 GAQPHRSIDPVLAAAQLTLAWQSIVTRNINPNHRAVISVTQLHTGTADNVIPEQATLSGT 259 Query: 264 IRTLSSTLQQKLQEDIRRMCHALADAYGAKVEVEFFQYYSATINTPTETDFCERVIRQTF 323 +R+ + ++ + + A+ + +V+ F + Y A +N P T + + Sbjct: 260 VRSFDAETLDLIEHRMAAIAEAIGQMFDLRVQFHFERLYPAVVNHPEATQQAMQAMMAAV 319 Query: 324 GDERIRTGIPANMTSEDFGFMLEERPGTYVLIGN--------APQDRASHSLHHPHYDFN 375 G E++ SEDF F L+ +PG Y +GN A S LH P YDFN Sbjct: 320 GAEQVDGDTEPAFASEDFAFYLQHKPGCYAFLGNGVIADGKQAGSQATSRELHSPFYDFN 379 Query: 376 DDIIEEGVRYWVALAQSWFG 395 DDII+ GV YWV+L + + G Sbjct: 380 DDIIDAGVAYWVSLVRQYLG 399 >gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoacylase/carboxypeptidase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 340 bits (871), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 172/383 (44%), Positives = 238/383 (62%), Gaps = 5/383 (1%) Query: 14 LHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVQELQALGYEVTRGLGGTGVVASLPGSD 73 LH + +RR LH HPEL FEE TA +V Q L+ GY VTR +GG G+VA+L Sbjct: 15 LHDIQDEITAIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRHIGGNGLVATLRVGS 74 Query: 74 PQRGIVLRADLDALPIVEANDFAHASCSHGIMHACGHDGHTVMLLGAARVLKQQPTLPGS 133 R I LRAD+DALPI E AS G MHACGHDGHT MLLGAAR L + G+ Sbjct: 75 SARSIGLRADMDALPIQEETGLDWASVKPGAMHACGHDGHTAMLLGAARHLARTRRFDGT 134 Query: 134 VHFVFQPGEEGG--AGARKMIDDGLFDQFPTEAVFGMHNWPGLPAGQFGLRTGPIMAAGS 191 ++ +FQP EE G +GA+KM+ DGLF++FP EAVFG+HN PG+ AG F R+GP MAA Sbjct: 135 LNLIFQPAEEAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAGTFMFRSGPFMAACD 194 Query: 192 RLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTD 251 +KI ITG+G+HAA+PHL +DP+ +A S+V+ QT+ +R+ DP+D AV++V HAG Sbjct: 195 TVKIRITGRGSHAARPHLSVDPVVVAASLVMALQTVVSRNIDPMDSAVVTVGSLHAGKAS 254 Query: 252 NVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCHALADAYGAKVEVEFFQYYSATINTPTE 311 NVIP+ A + ++R+ +++ L++ IR + A +YGA+ E+++ + Y +N+ E Sbjct: 255 NVIPEFATMELSVRSFKPEVRELLEQRIRALVSTHAQSYGAQAEIDYLRGYPVLVNSDAE 314 Query: 312 TDFCERVIRQTFGDERIRTGIPANMTSEDFGFMLEERPGTYVLIGNAPQDRASHSLHHPH 371 TDF V + G E++ SEDF + L +RPG ++ +GN + LH+ Sbjct: 315 TDFARSVAEELVGPEKVIAPFGPIAGSEDFAYFLRQRPGCFLRVGNG---QGKPMLHNAG 371 Query: 372 YDFNDDIIEEGVRYWVALAQSWF 394 YDFND I G YW L + W Sbjct: 372 YDFNDANIPIGAAYWTRLVERWM 394 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,457,008 Number of extensions: 66316 Number of successful extensions: 199 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 3 Length of query: 406 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 319 Effective length of database: 1,207,925 Effective search space: 385328075 Effective search space used: 385328075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)