BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0179.p (493 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 924 0.0 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 310 2e-85 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 297 1e-81 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 293 2e-80 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 276 4e-75 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 924 bits (2387), Expect = 0.0, Method: Compositional matrix adjust. Identities = 454/483 (93%), Positives = 466/483 (96%) Query: 11 SPLAHLKRNGRLDHFYIDGQWQRPQGDGVAAVVSPATEERVCEIALGNAADVDQAVQAAR 70 SPL HL RN RLDHFYIDG+WQ PQG AAVV+PATEERVCEIALGNAADVDQAVQAAR Sbjct: 4 SPLTHLTRNARLDHFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAAR 63 Query: 71 RAFPAWAATSPLERAALLGRVHALLVERTELFAQALTLEMGAPITYARTAHVPLAAEHLR 130 RA PAWAATSPLERAALLGRVHALL+ER+ELFAQALTLEMGAPI+YARTAHVPLAAEHLR Sbjct: 64 RAIPAWAATSPLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLR 123 Query: 131 VARDNLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPS 190 VARDNLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPS Sbjct: 124 VARDNLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPS 183 Query: 191 ELSPLSALLFAEIVHEAGIPAGVFNLVNGSGPEVGAAMSSHPQVDMISITGSTRAGVLVA 250 ELSPLSALLFAEIVHEAG+PAGVFNLVNGSG EVGAA+S+HPQVDMISITGSTRAGVLVA Sbjct: 184 ELSPLSALLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVA 243 Query: 251 QAAAVTVKRVAQELGGKSPNVVLPDADLARAIPPGVAAAFRNMGQSCSAPTRLIVPRAAL 310 QAAAVTVKRVAQELGGKSPN+VLPDADL RAIPPGVAAA RNMGQSCSAPTRLIVPRAAL Sbjct: 244 QAAAVTVKRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAAL 303 Query: 311 DRVHELALATLAQMKVGDPTDEATTHGPLANRAQFLRVQQMIEAGMQDGARLLAGGAGRP 370 RVHELALATLAQMKVGDP D ATTHGPLANRAQFLRVQQMIEAG+QDGARLLAGG GRP Sbjct: 304 ARVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRP 363 Query: 371 DGLEQGYYARPTIFSDVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQ 430 DGL QGYYARPTIFS+VHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQ Sbjct: 364 DGLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQ 423 Query: 431 GTDKDQVRAVAARIQSGQVHLNYPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKSILG 490 G DKDQVRAVAARIQSGQVHLNYPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVK+ILG Sbjct: 424 GRDKDQVRAVAARIQSGQVHLNYPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKAILG 483 Query: 491 YYA 493 YYA Sbjct: 484 YYA 486 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 310 bits (793), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 181/471 (38%), Positives = 266/471 (56%), Gaps = 14/471 (2%) Query: 26 YIDGQWQRPQGDGVAAVVSPATEERVCEIALGNAADVDQAVQAARRAFPAWAATSPLERA 85 +++GQW AV +PA E + + L AA+ +A++AA A+PAW + ER+ Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERS 74 Query: 86 ALLGRVHALLVERTELFAQALTLEMGAP-------ITYARTAHVPLAAEHLRVARDNLAD 138 A+L R + L++ T+ A +T E G P I YA + A E R D + Sbjct: 75 AILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTI-- 132 Query: 139 YPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSAL 198 P P +++E IGVCA ITPWN+P IT K GPALAAGC +VLKP+E +P SAL Sbjct: 133 -PSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSAL 191 Query: 199 LFAEIVHEAGIPAGVFNLVNGSGPEVGAAMSSHPQVDMISITGSTRAGVLVAQAAAVTVK 258 A + AGIPAGVF++V G+ +G M+S+P V IS TGST G L+ Q +A ++K Sbjct: 192 ALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIK 251 Query: 259 RVAQELGGKSPNVVLPDADLARAIPPGVAAAFRNMGQSCSAPTRLIVPRAALDRVHELAL 318 +++ ELGG +P +V DADL A+ +A+ +RN GQ+C R+ V D + Sbjct: 252 KLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLV 311 Query: 319 ATLAQMKVGDPTDEATTHGPLANRAQFLRVQQMIEAGMQDGARLLAGGAGRPDGLEQGYY 378 + + KVG T+E T GPL N +V+Q + + GAR+L G G+ L ++ Sbjct: 312 EAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLG--GKRHALGHSFF 369 Query: 379 ARPTIFSDVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGTDKDQVR 438 PT+ +DV M +A+EE FGP+ + + T EE +A+ANDT +GL ++ D ++ Sbjct: 370 -EPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIW 428 Query: 439 AVAARIQSGQVHLNYPAWDPQ-APFGGYKQSGNGREYGVEGMEEYMEVKSI 488 VA ++SG V +N + APFGG KQSG GRE G+++Y+ VK + Sbjct: 429 RVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYL 479 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 297 bits (761), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 178/423 (42%), Positives = 236/423 (55%), Gaps = 12/423 (2%) Query: 75 AWAATSPLERAALLGRVHALLVERTELFAQALTLEMGAPITYARTAHVPLAAEHLRVARD 134 AW+ S L+R LL ++ AL+ T+L A +G R VP A HLR A Sbjct: 59 AWSKLSGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAG 118 Query: 135 --------NLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVV 186 + ++ + VREPIGV I PWN P+ + KV LAAGCTVV Sbjct: 119 WANQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVV 178 Query: 187 LKPSELSPLSALLFAEIVHEAGIPAGVFNLVNGSGPEVGAAMSSHPQVDMISITGSTRAG 246 +KP+E +P SAL A + EAG P GV N+V G G VG A+ HP V IS TGS AG Sbjct: 179 IKPAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAG 238 Query: 247 VLVAQAAAVTVKRVAQELGGKSPNVVLPDADLARAIPPGVAAAFRNMGQSCSAPTRLIVP 306 + + A KRV ELGGKSP +V DA A+ F N GQ C+A +R++V Sbjct: 239 RAIQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQ 298 Query: 307 RAALDRVHELALATLAQMKVGDPTDEATTHGPLANRAQFLRVQQMIEAGMQDGARLLAGG 366 R+ R + A + VGDP+ GP+A +AQF RV + I+ G+ +GA LLAGG Sbjct: 299 RSLAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGG 358 Query: 367 AGRPDGLEQGYYARPTIFSDVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLG 426 RPD +G++ +PTIF+D + M IA+EEIFGPV I+ +DT EEAIA+AND+ YGL Sbjct: 359 VSRPD---KGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLA 415 Query: 427 AHVQGTDKDQVRAVAARIQSGQVHLN-YPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEV 485 A V T+ + VAA ++ G V +N + D P+GG K SG GRE G+ G Y E Sbjct: 416 ATVWTTNLARAHRVAAGVKVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEE 475 Query: 486 KSI 488 K++ Sbjct: 476 KTV 478 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 293 bits (750), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 184/485 (37%), Positives = 262/485 (54%), Gaps = 15/485 (3%) Query: 21 RLDHFYIDGQWQRPQGDGVAAVVSPATEERVCEIALGNAADVDQAVQAARRAFPAWAATS 80 R D+F I G++ P +SP CE+A +A DV+ A+ AA A +W TS Sbjct: 18 RYDNF-IGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKSWGKTS 76 Query: 81 PLERAALLGRVHALLVERTELFAQALTLEMGAPITYARTAHVPLAAEHLR----VARDNL 136 P ERA +L ++ + EL A A TL+ G PI A +PLA +H R R Sbjct: 77 PTERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQE 136 Query: 137 ADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLS 196 I EP+GV I PWN+PI K+ PALAAG VVLKP+E +P S Sbjct: 137 GSICPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPAS 196 Query: 197 ALLFAEIVHEAGIPAGVFNLVNGSGPEVGAAMSSHPQVDMISITGSTRAGVLVAQAAAVT 256 ++ E++ + IP GV N+V G G E G ++S+ ++ I+ TG T G L+ Q A+ Sbjct: 197 IMVLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQN 255 Query: 257 VKRVAQELGGKSPNVVLPDA-----DLARAIPPGVAAAFRNMGQSCSAPTRLIVPRAALD 311 + V ELGGKSPN+ D D G A N G+ C+ P+R++V + + Sbjct: 256 LIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFALNQGEVCTCPSRVLVQESIYE 315 Query: 312 RVHELALATLAQMKVGDPTDEATTHGPLANRAQFLRVQQMIEAGMQDGARLLAGGAGRPD 371 R E AL +A +K G+P D++T G A++ Q ++ I+ G Q+GA++LAGG Sbjct: 316 RFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGREEL 375 Query: 372 G--LEQGYYARPTIFSDVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHV 429 G L GYY +PT+F + M I QEEIFGPV+++ + EEA+AIANDT+YGLGA + Sbjct: 376 GGDLASGYYVKPTVFQG-NNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGAGL 434 Query: 430 QGTDKDQVRAVAARIQSGQVHLN-YPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKSI 488 D + + IQ+G+V N Y + A FGGYKQSG GRE ++ Y + K++ Sbjct: 435 WTRDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKNL 494 Query: 489 LGYYA 493 L Y+ Sbjct: 495 LVSYS 499 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 276 bits (705), Expect = 4e-75, Method: Compositional matrix adjust. Identities = 163/472 (34%), Positives = 250/472 (52%), Gaps = 12/472 (2%) Query: 24 HFYIDGQWQRPQGDGVAAVVSPATEERVCEIALGNAADVDQAVQAARRAFPAWAATSPLE 83 +I+G+W V++PATEE + +IA + AD+D+A++AA + F W S E Sbjct: 6 QLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFE 65 Query: 84 RAALLGRVHALLVERTELFAQALTLEMGAPITYARTAHVPLA------AEHLRVARDNLA 137 R+ ++ R LL ER E A+ +T+E G P+ A+ + A AE R L Sbjct: 66 RSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLV 125 Query: 138 DYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSA 197 P P +++EP+G A TPWN+P+ Q+ K+ ALAAGC++++K E +P S Sbjct: 126 --PARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASP 183 Query: 198 LLFAEIVHEAGIPAGVFNLVNGSGPEVGAAMSSHPQVDMISITGSTRAGVLVAQAAAVTV 257 +AG+PAGV NLV G E+ + HP + IS TGST G +A A + Sbjct: 184 AELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHM 243 Query: 258 KRVAQELGGKSPNVVLPDADLARAIPPGVAAAFRNMGQSCSAPTRLIVPRAALDRVHELA 317 KR ELGG +P +V DAD+ A A FRN GQ C +PTR +V + + + Sbjct: 244 KRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKF 303 Query: 318 LATLAQMKVGDPTDEATTHGPLANRAQFLRVQQMIEAGMQDGARLLAGGAGRPDGLEQGY 377 + Q+KVG+ + T GP+AN+ + ++ +I+ + GA+L GG + +GY Sbjct: 304 VTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGN---KGY 360 Query: 378 YARPTIFSDVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGTDKDQV 437 + +PT+ +++ EE FGP+ I ++T++E I AN YGL A+ Sbjct: 361 FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTK 420 Query: 438 RAVAARIQSGQVHLNYPAWD-PQAPFGGYKQSGNGREYGVEGMEEYMEVKSI 488 A+AAR++SG + +N+ P+ PFGG K SG G E G E +E Y+ K + Sbjct: 421 HALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLV 472 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,490,441 Number of extensions: 62310 Number of successful extensions: 305 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 236 Number of HSP's successfully gapped: 22 Length of query: 493 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 404 Effective length of database: 1,198,455 Effective search space: 484175820 Effective search space used: 484175820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)