BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0162.p (308 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310460|ref|YP_003774552.1| D-3-phosphoglycerate dehydrogen... 584 e-168 gi|300311801|ref|YP_003775893.1| D-isomer specific 2-hydroxyacid... 147 9e-37 gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 82 4e-17 gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogen... 80 3e-16 gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogen... 77 1e-15 >gi|300310460|ref|YP_003774552.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 584 bits (1506), Expect = e-168, Method: Compositional matrix adjust. Identities = 287/308 (93%), Positives = 298/308 (96%) Query: 1 MTFLYKADPVRGAEWAQLFAEKAPELPFHIWPHEGDGSSVRYLAAWTPPEDIAQHFPHLE 60 MTFLYKADPVRGAEWAQLFA+KAPELPF IWPH+GDG+SVRYLAAWTPP+DIA+ FP LE Sbjct: 1 MTFLYKADPVRGAEWAQLFAQKAPELPFQIWPHDGDGASVRYLAAWTPPDDIAERFPKLE 60 Query: 61 ILFSVGAGIDQFDMSTLPPDLPVVRMTEPGIAAGMVQYVTHAVLGLHRDSRIYARQQRDQ 120 ILFSVGAGIDQFDMSTLPP LPVVRMTEPGIAAGMVQYVTHAVLGLHRDSR+YARQQRDQ Sbjct: 61 ILFSVGAGIDQFDMSTLPPALPVVRMTEPGIAAGMVQYVTHAVLGLHRDSRVYARQQRDQ 120 Query: 121 VWRAHRVRPASACRVGVLGLGMLGRAVLQQLHGLGFPCAGWSRSAHQMDGIDCYSGSDGL 180 VWRAHRVRPASACRVGVLGLGMLGRAVLQQLHGLGFPCAGWSRSAHQMDGIDCYSGS GL Sbjct: 121 VWRAHRVRPASACRVGVLGLGMLGRAVLQQLHGLGFPCAGWSRSAHQMDGIDCYSGSAGL 180 Query: 181 RDFLARTDVLVCLVPLTDDTRGLLNRELFAQLPRGAALVNVGRGGHLVEDDLLAALNDGQ 240 R FLARTDVLVCLVPLT++TRGLL R+LFAQLPRGAAL+NVGRGGHLVEDDLLAALNDGQ Sbjct: 181 RAFLARTDVLVCLVPLTEETRGLLCRDLFAQLPRGAALINVGRGGHLVEDDLLAALNDGQ 240 Query: 241 LSAAVLDVCETEPLPQGHPFWTHPDIVLTPHIASMTQPDGAVDAVLENLRRHRAGLPLLG 300 LSAAVLDVCE EPLP GHPFWTHPDIVLTPHIASMTQPDGAVDAVLENLRR+R GLPLLG Sbjct: 241 LSAAVLDVCEVEPLPPGHPFWTHPDIVLTPHIASMTQPDGAVDAVLENLRRYREGLPLLG 300 Query: 301 LIDRQRGY 308 LIDRQRGY Sbjct: 301 LIDRQRGY 308 >gi|300311801|ref|YP_003775893.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 319 Score = 147 bits (372), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 102/255 (40%), Positives = 141/255 (55%) Query: 54 QHFPHLEILFSVGAGIDQFDMSTLPPDLPVVRMTEPGIAAGMVQYVTHAVLGLHRDSRIY 113 + P+L + SV AG+++ DL + R+ +P +AA M + V L LHRD +Y Sbjct: 65 KRMPNLLWVHSVWAGVERLVADLGQSDLQLTRLVDPRLAATMAEAVLAWTLYLHRDMPLY 124 Query: 114 ARQQRDQVWRAHRVRPASACRVGVLGLGMLGRAVLQQLHGLGFPCAGWSRSAHQMDGIDC 173 AR+QR+ W A RV +LGLG LG A L F GWSRSA + +G+ Sbjct: 125 ARRQREHHWEAQPYVLPEQRRVSLLGLGALGEAAAAALRQARFAVNGWSRSAREAEGVQT 184 Query: 174 YSGSDGLRDFLARTDVLVCLVPLTDDTRGLLNRELFAQLPRGAALVNVGRGGHLVEDDLL 233 + G GL LA+TD+LVCL+PLT +TRGL++ A LP A L+N RG + + L Sbjct: 185 FHGPAGLAQMLAQTDILVCLLPLTAETRGLIDAAHLAMLPPQAQLINFARGPIIDDAALK 244 Query: 234 AALNDGQLSAAVLDVCETEPLPQGHPFWTHPDIVLTPHIASMTQPDGAVDAVLENLRRHR 293 AAL+ GQL AVLDV EPLP W+HP + + PH ++ T A V N+RR+R Sbjct: 245 AALDAGQLRHAVLDVFAVEPLPPAAWPWSHPAVTVLPHCSAPTDRQTASAIVAANIRRYR 304 Query: 294 AGLPLLGLIDRQRGY 308 + +D +RGY Sbjct: 305 HSGEIAANVDLRRGY 319 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 82.4 bits (202), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 62/227 (27%), Positives = 96/227 (42%), Gaps = 6/227 (2%) Query: 57 PHLEILFSVGAGIDQFDMSTLPPDLPVVRMTEPGIAAGMVQYVTHAVLGLHRDSRIYARQ 116 P L+I+ ++ G+D FD+ ++ T + + +L R A Sbjct: 65 PQLKIVSTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILASARRVVELAEY 124 Query: 117 QRDQVWRAHRVRPASACRV-----GVLGLGMLGRAVLQQL-HGLGFPCAGWSRSAHQMDG 170 + W+ V G++G+G +G AV ++ HG G P +R Sbjct: 125 VKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNRRPDPEAE 184 Query: 171 IDCYSGSDGLRDFLARTDVLVCLVPLTDDTRGLLNRELFAQLPRGAALVNVGRGGHLVED 230 + + D LA+ D + ++PLT T + FA + R A +N RG + E Sbjct: 185 RELGARYVSKEDLLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFINASRGRIVDEA 244 Query: 231 DLLAALNDGQLSAAVLDVCETEPLPQGHPFWTHPDIVLTPHIASMTQ 277 L+AAL D + A LDV E EPLP P P++V PHI S T Sbjct: 245 ALIAALQDKTIHGAGLDVFEVEPLPLQSPLLKLPNVVALPHIGSATH 291 >gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 310 Score = 79.7 bits (195), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 73/243 (30%), Positives = 116/243 (47%), Gaps = 17/243 (6%) Query: 56 FPHLEILFSVGAGIDQFDMSTLPPDLPVVRMTEPGIAAGMVQ--YVTHAV---LGLHRDS 110 PHL+++ ++GAG + D++ E AG + HA+ L + R+ Sbjct: 64 LPHLQLVCTLGAGFENVDVAHAE-----AHGIEIATGAGTNEDCVADHALGLLLAILRNI 118 Query: 111 RIYARQQRDQVWRAHR-VRPASA-CRVGVLGLGMLGRAVLQQLHGLGFPCAGWSRSAHQM 168 + R RD WR ++P A RVG++G+G +G+ + ++ A +R + Sbjct: 119 PVLDRYTRDGGWRETIPLQPQLAGKRVGIVGMGNIGKKIARRAAAFDAEIAYCNRK--KR 176 Query: 169 DGIDCYSGSDGLRDFLARTDVLVCLVPLTDDTRGLLNRELFAQLPRGAALVNVGRGGHLV 228 D +D + D + D L+ P TR L+N + +L LVN+GRG + Sbjct: 177 DDVDYHYFPD-VAQLAGWADCLIVAAPGGAQTRHLINARVLEELGPQGYLVNIGRGSIVD 235 Query: 229 EDDLLAALNDGQLSAAVLDVCETEPLPQGHPFWTHPDIVLTPHIASMTQPDGAVDAVLEN 288 D L AAL+ G+L+ A LDV E EP P P++VLTPHIA + P+ +V + Sbjct: 236 TDALGAALSSGRLAGAGLDVYEGEPQPPA-ALIALPNVVLTPHIAGWS-PEAIRASVTQF 293 Query: 289 LRR 291 LR Sbjct: 294 LRN 296 >gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 77.4 bits (189), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 52/145 (35%), Positives = 77/145 (53%), Gaps = 9/145 (6%) Query: 135 VGVLGLGMLGRAVLQQLHGLGFPCAG---WSRSAHQMDGIDCYSGSDGLRDFLARTDVLV 191 +G++GLG +GR V LG P +++ A Q G+ L A++DV+ Sbjct: 149 LGLVGLGAIGRRVAAVAAALGMPVLAHDPYAKEAPQ--GVQLVD----LDTLFAQSDVVS 202 Query: 192 CLVPLTDDTRGLLNRELFAQLPRGAALVNVGRGGHLVEDDLLAALNDGQLSAAVLDVCET 251 PLT + + ++N + A++ GA LVN RGG + E L+AAL+ G+L AA LD E Sbjct: 203 LHCPLTAENKHMINAQSLARMKDGAILVNTARGGLIDEQALIAALDSGKLRAAGLDSFEK 262 Query: 252 EPLPQGHPFWTHPDIVLTPHIASMT 276 EP HP + VL+PHI +T Sbjct: 263 EPFTAPHPLQRVGNAVLSPHIGGVT 287 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.141 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,107,469 Number of extensions: 49894 Number of successful extensions: 140 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 135 Number of HSP's successfully gapped: 11 Length of query: 308 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 223 Effective length of database: 1,217,395 Effective search space: 271479085 Effective search space used: 271479085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)