BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0161.p (430 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310459|ref|YP_003774551.1| glycine/D-amino acid oxidase [H... 798 0.0 gi|300309600|ref|YP_003773692.1| glycine/D-amino acid oxidase [H... 457 e-130 gi|300310497|ref|YP_003774589.1| glycine/D-amino acid oxidase [H... 177 1e-45 >gi|300310459|ref|YP_003774551.1| glycine/D-amino acid oxidase [Herbaspirillum seropedicae SmR1] Length = 430 Score = 798 bits (2062), Expect = 0.0, Method: Compositional matrix adjust. Identities = 392/430 (91%), Positives = 414/430 (96%) Query: 1 MKLESYWTDSAPLFISPTRELPTQVDVAIVGGGFTGLSAALAFAKRGASVVVLEAGPHVA 60 MKLESYWTDSAPL+ISPTR+LP QVDVAIVGGGFTGLSAALA A+RGASVVVLEAGPHVA Sbjct: 1 MKLESYWTDSAPLYISPTRDLPAQVDVAIVGGGFTGLSAALALARRGASVVVLEAGPHVA 60 Query: 61 AEASGRNGGHVNNGLAVDYAEVAAKVGVERARAWYHAYDDAVDTVARLVEQEQIACDFLR 120 AEASGRNGGHVNNGLAVDYAE+AAKVGVERARAWYHAYDDAVDTVARLV++EQI CDFLR Sbjct: 61 AEASGRNGGHVNNGLAVDYAELAAKVGVERARAWYHAYDDAVDTVARLVQEEQIDCDFLR 120 Query: 121 HGKLKMATKPYQMDALQRSADRLLADGVDSDVEILDAARVRAEVQSERFHGGLLYKRSGQ 180 HGKLKMAT+PYQMDALQRSADRL+ADGVD DVEILDA RV+AEVQSERFHGGLLYKRSGQ Sbjct: 121 HGKLKMATRPYQMDALQRSADRLIADGVDLDVEILDAQRVQAEVQSERFHGGLLYKRSGQ 180 Query: 181 MHMGRFAWGLATAAERHGAAIHTGTEVRRLERIGDTHAHRVHTARGTITAKQVLLATGAS 240 MHMGRFA+GLA AAER GAAIHTGTEVRRLERIG TH HR+HT RG + A+QVLLATGAS Sbjct: 181 MHMGRFAYGLAMAAERQGAAIHTGTEVRRLERIGQTHGHRLHTTRGAVLARQVLLATGAS 240 Query: 241 RHGAYGSFGWLRRRIVPIGSFIVVTEPLGAERAAAMLARQRTYTTVANIHHYFRMTPDTR 300 RHGAYGSFGWLRRRIVPIGSFIVVTEPLGA+RAA +L QRTYTTVANIHHYFRMTPDTR Sbjct: 241 RHGAYGSFGWLRRRIVPIGSFIVVTEPLGAQRAATLLKHQRTYTTVANIHHYFRMTPDTR 300 Query: 301 LVFGGRARFAISSPQSDAASGEILRQGLHETFPQLGQVRLDYCWGGLVDMTRDRLPQAGE 360 LVFGGRARFAISSPQSDAASGE+LR+GLHETFPQLGQVRLDYCWGGLVD+TRDRLPQAGE Sbjct: 301 LVFGGRARFAISSPQSDAASGEVLRRGLHETFPQLGQVRLDYCWGGLVDVTRDRLPQAGE 360 Query: 361 RDGLYYSMGYSGHGTQMSVHMGQCMAQVMAGDALANPWREREWPAIPGHFGPPWFLPVIG 420 RDGLYYSMGYSGHGTQMSVHMGQCMA+VMAGDALANPWREREWPAIPGHFG PWFLP++G Sbjct: 361 RDGLYYSMGYSGHGTQMSVHMGQCMAKVMAGDALANPWREREWPAIPGHFGAPWFLPLVG 420 Query: 421 LYYQLKDKLA 430 LYY+LKDKLA Sbjct: 421 LYYRLKDKLA 430 >gi|300309600|ref|YP_003773692.1| glycine/D-amino acid oxidase [Herbaspirillum seropedicae SmR1] Length = 425 Score = 457 bits (1176), Expect = e-130, Method: Compositional matrix adjust. Identities = 242/430 (56%), Positives = 301/430 (70%), Gaps = 8/430 (1%) Query: 1 MKLESYWTDSAPLFISPTRELPT-QVDVAIVGGGFTGLSAALAFAKRGASVVVLEAGPHV 59 M+L+SYW D++P F+S T+ LP DV +VGGG TG +AALA A++GA VVV EA V Sbjct: 2 MQLKSYWLDTSPAFVSQTQTLPEGACDVVVVGGGITGSAAALALARKGARVVVCEADI-V 60 Query: 60 AAEASGRNGGHVNNGLAVDYAEVAAKVGVERARAWYHAYDDAVDTVARLVEQEQIACDFL 119 ASGRNGG NNG A DYA ++ ++GVE A Y A+D VDTV RLV +E I C F Sbjct: 61 GGAASGRNGGMCNNGFAQDYATLSQRLGVEWANRLYRAFDAGVDTVERLVREESIDCSFA 120 Query: 120 RHGKLKMATKPYQMDALQRSADRLLADGVDSDVEILDAARVRAEVQSERFHGGLLYKRSG 179 RHGKLK+A KP +D L RS + LLA VD D +L A++R E+ S+R+HGGLL +S Sbjct: 121 RHGKLKLAAKPEHVDKLARSQE-LLARHVDGDTRLLSRAQLRDELGSDRYHGGLLMHKSA 179 Query: 180 QMHMGRFAWGLATAAERHGAAIHTGTEVRRLERIGDTHAHRVHTARGTITAKQVLLATGA 239 MH+GR+ GLA AA+R GA T V +E +A V T+ G + A QVLLA+G Sbjct: 180 GMHVGRYVRGLAQAAQRRGALYLERTPVLGIEAKNGRYA--VRTSAGVLHAGQVLLASGI 237 Query: 240 SRHGAYGSFGWLRRRIVPIGSFIVVTEPLGAERAAAMLARQRTYTTVANIHHYFRMTPDT 299 S+ G FGW+RRRIVP+G+F++VTEPL +L QR YT N +YFR TPD Sbjct: 238 SQ---VGPFGWIRRRIVPVGAFLIVTEPLPGALLKQLLPTQRMYTDTKNFVNYFRATPDQ 294 Query: 300 RLVFGGRARFAISSPQSDAASGEILRQGLHETFPQLGQVRLDYCWGGLVDMTRDRLPQAG 359 RL+FGGRARFA S+PQSDA SGEILR + E FP L Q R+DYCWGG+VDMT DRLP+AG Sbjct: 295 RLLFGGRARFAASNPQSDARSGEILRAQMLEVFPALAQTRIDYCWGGMVDMTTDRLPRAG 354 Query: 360 ERDGLYYSMGYSGHGTQMSVHMGQCMAQVMAGDALANPWREREWPAIPGHFGPPWFLPVI 419 +RDGLYYSMGYSGHGT M+ MG MA++M G A NPW++ +WPAIPGHFG PWFLP + Sbjct: 355 QRDGLYYSMGYSGHGTHMATLMGSMMAEIMDGHAELNPWKDFDWPAIPGHFGKPWFLPFV 414 Query: 420 GLYYQLKDKL 429 G +Y+LKD+L Sbjct: 415 GAWYRLKDRL 424 >gi|300310497|ref|YP_003774589.1| glycine/D-amino acid oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 177 bits (450), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 148/436 (33%), Positives = 211/436 (48%), Gaps = 24/436 (5%) Query: 5 SYWTDSAPLFI-SPTRELPTQVDVAIVGGGFTGLSAALAFAKRGASVVVLEAGPHVAA-E 62 S W +A F +P + + DVAIVG G+TGLS AL A+ G SV VL+A H Sbjct: 12 SLWAATAGEFSGAPPLQASARYDVAIVGAGYTGLSTALHLAQAGVSVCVLDA--HAPGWG 69 Query: 63 ASGRNGGHVNNGLAVDYAEVAAKVGVERARAWYHAYDDAVDTVARLVEQEQIACDFLRHG 122 ASGRNGG V GL D ++ G A A A DTV L+ + I C+ R G Sbjct: 70 ASGRNGGQVIPGLKYDPDQLRVMFGSAVADPLIAAIGSAADTVFDLIARHGIECEAQRAG 129 Query: 123 KLKMATKPYQMDALQRSADRLLADGVDSDVEILDAARVRAEVQSERFHGGLLYKRSGQMH 182 ++ P M AL+ A + +A+G + +++D A V + + + GG +R+G +H Sbjct: 130 WIQPTHSPKMMRALEARARQWMAEG--APAQLMDGAEVTRRIGTGAYVGGWKDERAGSLH 187 Query: 183 MGRFAWGLATAAERHGAAIHTGTEVRRLERIGDTHAHRVHTARGT----ITAKQVLLATG 238 ++ GLA AA+R G IH T V RLER R+H G I A++V+LAT Sbjct: 188 PLKYCRGLAQAAQRLGVVIHGDTRVTRLER--RQGGWRLHGPSGEQGPHIDAERVVLAT- 244 Query: 239 ASRHGAYGSFGW--LRRRIVPIGSFIVVTEPLGAERAAAMLARQRTYTTVANIHHYFRMT 296 Y W LR+ ++ SFIV T PL A++L + + Y+R Sbjct: 245 ----NGYTDDLWPHLRQSVIAANSFIVATRPLPPALGASILPGGEVTSDSRRLLLYYRRD 300 Query: 297 PDTRLVFGGRARFAISSPQSDAASGEILRQGLHETFPQLGQVRLDYCWGGLVDMTRDRLP 356 RL+ GGR F +D A L + + FPQL V +Y W G V +TRD LP Sbjct: 301 AQGRLLMGGRGPFGEPQGPADFAH---LERSVALLFPQLAGVEYEYRWSGRVAITRDFLP 357 Query: 357 QAGER-DGLYYSMGYSGHGTQMSVHMGQCMAQVMAGDA-LANPWREREWPAIPGHFGPPW 414 E GL ++GY+G G M+ MGQ +AQ + G A A P+ IP H + Sbjct: 358 HVHEPAPGLSIALGYNGRGIAMATMMGQRLAQRLIGPAGTAFPFPVSTIAPIPLHGLQRF 417 Query: 415 FLPVIGLYYQLKDKLA 430 ++ +Y + D+ + Sbjct: 418 YIAAGVAWYSVLDRFS 433 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,476,652 Number of extensions: 65946 Number of successful extensions: 294 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 283 Number of HSP's successfully gapped: 4 Length of query: 430 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 342 Effective length of database: 1,203,190 Effective search space: 411490980 Effective search space used: 411490980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)